THE ANTICANCER EFFECT AND ANTI－DNA TOPOISOMERASE II EFFECT OF EXTRACTS OF CAMELLIA PTILOPHYLLA CHANG AND CAMELLIA SINENSIS

The cytotoxic effect of extract of camellia ptilophyllachang(ECPC) and extract of camellia sinensis(ECS) onHeLa cell line, poorly differentiated nasopharyngealcarcinoma cell line(CNE2) and gastric cancer cell line(MGC-803 ) in vitro was studied using MIT assay method.The results showed that ECPC and ECS possessed significantcytotoxic effect on above three cell lines. The anticancer testin mice showed that ECPC had marked inhibitory effectagainst Ehrlich solid carcinoma(ESC) with inhibition ratesof 17. 8 48. 3% and with inhibition rates of 28. 3-54. 5% against reticular cell sarcoma(L2), and that ECShad inhibition rates of 31 . 5 -49. 4 % against ESC and 35. 8- 50% against L2. These two extracts had only marginalinhibitory effect against sarcoma- 180. The unknottingactivity of DNA topoisomerase II was inhibited completelyby ECPC and ECS at the concentration of 50 μg/ mlsuggesting that DNA topoisomerase II might be a targetenzyme of these two extracts.

A Primary Investigation on Serum CTX-II Changes in Patients Infected with Brucellosis in Qinghai Plateau, China

Brucellosis is one of the most widespread zoonotic diseases, with the most frequent complication being osteoarticular changes. The aim of this study was to assess the changes of C-terminal telopeptide of type II collagen （CTX-II） in patients infected with brucellosis. A total of 84 brucellosis patients and 43 volunteers were selected and divided into brucellosis vs. control groups. Serum samples were subjected to serological tests for brucellosis,

Changes in the levels of CAM kinase II and synapsin I caused by oxidative stress in the rat brain, and its prevention by vitamin E

To define whether oxidative stress and aging induce abnormal dissociation of neurotransmitter-enclosing synaptic vesicles in rat brain nerve terminals, we assessed the activation of Ca+/calmodulin dependent protein kinase II (CAM kinase II) and changes in the levels of synapsin I, which is a synaptic vesicle-associated protein involved in the modulation of neurotransmitter release. Assessment of young rats subjected to hyperoxia-induced oxidative stress and normal aged rats revealed that synaptic CAM kinase II in the rat brain was markedly activated through oxidative stress and aging. In accordance with the activation of CAM kinase II, the levels of phosphorylated synapsin I increased significantly in nerve terminals. Furthermore, it was found that vitamin E prevents these oxidative stress-induced abnormal processes in rat nerve terminals. These results suggest that oxidative stress and aging facilitate the mobilization of neurotransmitter-enclosing synaptic vesicles from the reserve pool in the nerve terminal, thereby inducing abnormal accumulation of synaptic vesicles in the synapse, and that vitamin E inhibits this process in the brain through its antioxidative action.

Duncan-Chang E-υ模型在ADINA软件中的开发与应用

ADINA软件具有计算速度快、精度高和前后处理简单方便的优点,广泛应用于各类工程领域.然而,ADINA软件中没有岩土工程领域常用的Duncan-Chang模型.以土工数值分析中广泛应用的Duncan-Chang E-υ模型为例,介绍了ADINA软件中进行本构模型二次开发的方法,并给出了模型开发的方法和步骤.基于ADINA软件,以Duncan-Chang E-υ模型对某土石坝工程填筑过程进行了三维有限元计算,根据计算成果对坝体竣工期位移变形规律进行了分析.

IIS环境下Web站点文件安全远程监测系统的设计

IIS Web站点的文件安全是一个令人难以放心的问题.利用微软提供的FSO(File System Object)可编写基于Web的远程文件管理程序,但是由于它的开放性也为安全带来隐患.通过调用自主研制的组件来检测站点文件的变化,并给予报警和处理,可实现Web站点文件状况的远程监控.同时,系统还支持定时检测处理.

慢性荨麻疹患者血清PAF、抗-CCD IgE水平变化及临床意义分析

目的探讨慢性荨麻疹患者血清血小板活化因子(platelet activating factor,PAF)、抗交叉反应性糖类决定簇免疫球蛋白E(anti-cross reactive carbohydrate determinants immunoglobulin E,抗-CCD IgE)水平变化及临床意义,为临床诊断及病情程度、预后评估提供参考依据。方法选取2021年5月—2023年5月广州市皮肤病防治所收治的100例慢性荨麻疹患者,根据荨麻疹活动评分分为轻度组(n=41)、中度组(n=35)和重度组(n=24)。采集患者空腹静脉血,通过酶联免疫吸附法测定血清PAF水平,通过斑点免疫印迹法测定抗-CCD IgE、过敏原特异性免疫球蛋白E(specific immunoglobulin E,sIgE)。比较不同病情程度慢性荨麻疹患者血清PAF水平,分析慢性荨麻疹患者血清PAF水平与荨麻疹活动评分相关性,比较不同临床特征慢性荨麻疹患者抗-CCD IgE阳性率。结果中度组、重度组患者血清PAF水平高于轻度组,重度组患者血清PAF水平高于中度组,差异有统计学意义(P<0.05)。Pearson相关分析结果显示,慢性荨麻疹患者血清PAF水平与荨麻疹活动评分呈正相关性(r=0.491,P<0.001)。年龄≥45岁、重度病情、sIgE阳性患者抗-CCD IgE阳性率高于年龄<45岁、轻度病情、sIgE阴性患者,差异有统计学意义(P<0.05)。抗-CCD IgE阳性患者中过敏原阳性最高的为尘螨组合(66.67%),其次为蟑螂、螃蟹、普通豚草,分别为55.56%、44.44%和44.44%。结论慢性荨麻疹患者血清PAF水平与病情密切相关,且抗-CCD IgE水平与sIgE水平有一定关系,抗-CCD IgE阳性表达情况对sIgE试验存在一定干扰,分析试验结果时应考虑抗-CCD IgE的影响。

Biochemical changes in phenols,flavonoids,tannins,vitamin E,β-carotene and antioxidant activity during soaking of three white sorghum varieties

Objective:To investigate the changes in total phenols,flavonoids,tannins,vitamin E, β-carotene and antioxidant activity during soaking of three white sorghum varieties.Methods: The changes in total phenols,total ilavonoids,tannins,phenolic acids compounds,flavonoid components,vitamin E,P-carotene and antioxidant activity during soaking of sorghum grains were determined.Results:Total phenols,total flavonoids,tannins,vitamin E,P-carotene and antioxidant activity in raw sorghum were ranged from 109.21 to 116.70,45.91 to 54.69,1.39 to 21.79 mg/100 g,1.74 to 5.25,0.54 to 1.19 mg/kg and 21.72%to 27.69%and 25.29%to 31.97%,respectively. The above measured compounds were significantly decreased after soaking.p-Hydroxybenzoic acid,vanillic acid,syringic acid and cinnamic acid represent the major phenolic acids in Dorado variety.While ferulic acid,p-coumaric acid,gallic acid and caffeic acid represent the major phenolic acids in Shandaweel-6.On the other hand,protocatechuic acid represents the major phenolic acids in Giza-15.Regarding flavonoids components,Dorado was the highest variety in kampferol and naringenin while Shandaweel-6 was the highest variety in luteolin, apigenin,hypersoid,quercelin and christen.Finally,Giza-15 was the highest variety in catechin. Phenolic acids,flavonoid compounds and antioxidant activities were decreased after soaking. Conclusions:Sorghum varieties have moderate quantities from total phenols,total flavonoids, tannins,phenolic acids compounds,flavonoid components,vitamin E,P-carotene and antioxidant activity which decreased after soaking.

Vitamin E ameliorates aflatoxin-induced biochemical changes in the testis of mice

Aim: To assess the effect of aflatoxin on biochemical changes in the testis of mice and the possibility of ameliorationby vitamin E treatment. Methods: Adult male albino mice were orally administered with 25 or 50μg of aflatoxin/animal/day (750 or 1500 μg/kg body weight) for 45 days. The testis was isolated and processed for biochemical anal-ysis. Results; There was a significant, dose-dependent reduction in DNA, RNA, protein, sialic acid contents andthe activities of succinic dehydrogenase, adenosine triphosphatase and alkaline phosphatase in the testis of aflatoxin-treated mice as compared to the vehicle control. However, the acid phosphatase activity was significantly increased inthe aflatoxin-treated mice. Vitamin E (2 mg/animal/day) treatment significantly ameliorated the aflatoxin-inducedchanges, except the acid and alkaline phosphatase activities in the high dose group. Conclusion; Vitamin E treat-ment ameliorates the aflatoxin-induced changes in the testis of mice. (Asian J Androl 2001 Dec; 3: 305 - 309)

Distribution Prediction of Suitable Growth Area for Eucommia ulmoides in China under Climatic Change Background

[ Objective] The research aimed to study distribution prediction of suitable growth area for Eucommia ulmoides in China under climatic change background. [ Method] By using the maximum entropy model and many kinds of climate change scenarios, we predicted current and future distribution pattems of suitable growth area for Eucommia ulmoides in China and its change process. [ Result ] At present, highly suitable growth area of E. ulmoides mainly distributed in Sichuan, Shaanxi and Chongqing, Under climate change background, total suitable growth areas in future three decades all drastically reduced when compared with that at present. It was noteworthy that moderately and highly suitable growth areas of wild E. ulmoides all disappeared, and junction between Shaanxi and Gansu and Taibai Mountain would be stable suitable growth area of wild E. ulmoides. [ Condusioa] The research could provide useful reference data for investigation, protection and sustainable development of the wild E. ulmoides resources.

Assessment of Ecological Risk to Land Use in Liaoning Province Based on Climate Change

Based on the climatic data and other statistics of climatic data of 54 meteorological stations from 1956 to 2005 in Liangning Province,the annual and seasonal light,heat and water and the like major elements of climatic resources are diagnosed and analyzed by using the method of linear climate trend rate. The results show that warming trend is 0.25 ℃/10 a,the precipitation decrease gradually by 2.2 mm/a and the decreasing trend of solar radiation and hours of sunshine is not dramatic. The overall climate change trend is warming and drying,featuring "significantly increased temperature,the decreased precipitation and sunshine". These features are significant in autumn,thus leading to the trend of moving west and retreating east of the phenology boundaries. The results of scenario analysis on ecological security show that the ecological risk is spreading rapidly from the northwest to the east and to the south and form the southeastern and southern coastal line to the inland;different ecological land-use system have different ecological fragility. Specifically,the bare land and low coverage grassland have highest ecological vulnerability,while the forest and paddy filed have lowest ecological vulnerability. Therefore,suggestions on the ecological land use are put forward in terms of maintaining the overall ecological land use balance,saving water and adapting to climate change.

Effect of Decadal Changes in Air-Sea Interaction on the Climate Mean State over the Tropical Pacific

Collaboration of interannual variabilities and the climate mean state determines the type of E1 Nifio. Recent studies highlight the impact of a La Nifia-like mean state change, which acts to suppress the convection and low-level convergence over the central Pacific, on the predominance of central Pacific （CP） E1 Nifio in the most recent decade. However, how interannual variabilities affect the climate mean state has been less thoroughly investigated. Using a linear shallow-water model, the ef- fect of decadal changes of air-sea interaction on the two types of El Nifio and the climate mean state over the tropical Pacific is examined. It is demonstrated that the predominance of the eastem Pacific （EP） and CP E1 Nino is dominated mainly by relationships between anomalous wind stresses and sea surface temperature （SST）. Furthermore, changes between air-sea interactions from 1980-98 to 1999-2011 prompted the generation of the La Ninalike pattern, which is similar to the background change in the most recent decade.

Response of fuzzy clustering on different threshold determination algorithms in spectral change vector analysis over Western Himalaya, India

Abstract： Change detection is a standard tool to extract and analyze the earth＇s surface features from remotely sensed data. Among the different change detection techniques, change vector analysis （CVA） have an exceptional advantage of discriminating change in terms of change magnitude and vector direction from multispectral bands. The estimation of precise threshold is one of the most crucial task in CVA to separate the change pixels from unchanged pixels because overall assessment of change detection method is highly dependent on selected threshold value. In recent years, integration of fuzzy clustering and remotely sensed data have become appropriate and realistic choice for change detection applications. The novelty of the proposed model lies within use of fuzzy maximum likelihood classification （FMLC） as fuzzy clustering in CVA. The FMLC based CVA is implemented using diverse threshold determination algorithms such as double-window flexible pace search （DFPS）, interactive trial and error （T＆E）, and 3x3-pixel kernel window （PKW）. Unlike existing CVA techniques, addition of fuzzy clustering in CVA permits each pixel to have multiple class categories and offers ease in threshold determination process. In present work, the comparative analysis has highlighted the performance of FMLC based CVA overimproved SCVA both in terms of accuracy assessment and operational complexity. Among all the examined threshold searching algorithms, FMLC based CVA using DFPS algorithm is found to be the most efficient method.

Biochemical and Morphological Changes in the Lenses of Selenium and/or Vitamin E Deficient Rats

The activities of glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R),superoalde dismutase (SOD) and the contents of malondialdehyde (MDA) and free radicals were measured, and the morphological changes were observed in the lens of control rats, selenium-deficient (SeD) and/or vitamin E deficient (VED) rats. The activities of GSH-Px in the lens of SeD rats decreased significantly. The GSH-Px activities of lens were positively related to erythrocytes selenium level. There was a free radical at g = 2.0015 in the rat lens of all groupe, but the content of free radicals in the lens of SeD group was significantly higher than that of the control group. The free radical content of lens was negatively related to erythrocytes selenium level, as well as the GSH-Px activities in the lens. In vitro, ultraviolet radiation caused the generation of another kind of free radical (g = 2.0097) in the lens of all groups, but the amount of the free radical in the lens of the SeD group was also significantly higher than that of the control group. The activities of SoD and GSSG-R in VED rat lens were significantly decreased. The amount of MDA in the lens of SeD and/or VED rats were significantly increased. The results showed that the decrease of antioaldative capability in the lenses of SeD and/or VED rats accelerated the lipid peroxidation and generation of free radicals. Although only early morphological changes in SeD and/or VED rat lens were observed, it is considered that selenium and vitamin E deficiency may be involved in the occurrence of cataract

Chang'e 4 Lands at the Far Side of the Moon

Chang’e 4 began its travel to the moon at 02:23 Beijing time on December 8,2018 atop a LM-3B launch vehicle from the Xichang Satellite Launch Center.After separated from the carrier rocket,Chang’e 4 entered the Earth-moon transfer orbit with perigee of 200 km and apogee of 420 000 km.

Chang'e 3 Lander Completed Mechanical Test

Aground firing test of the Chang'e 3 lander was conducted recently to check the mechanical performance of the spacecraft. The test was vital before the propulsion system test of the Chang'e 3 lander and was also a very important precondition to verify whether the propulsion system design could meet the requirements for in-orbit operation.

Correlation analysis of human papillomavirus E6/E7 mRNA detection with diagnosis,prognosis and recurrence risk in patients with cervical epithelioma

BACKGROUND Cervical intraepithelial neoplasia(CIN)is an important precursor of cervical cancer.Early detection and treatment can reduce the incidence of cervical cancer.AIM To investigate the detection rate of human papillomavirus(HPV)E6/E7 mRNA in cervical tissue of patients with different types of epithelial cell neoplasia(CIN)and its relationship with CIN progression and diagnosis.METHODS One hundred women with HPV infection detected by cervical exfoliation cytology between January 2022 and January 2023 were retrospectively selected.These patients were graded CIN based on colposcopy and cervical pathology.The positive expression rates of HPV E6/E7 mRNA and HPV[polymerase chain reaction(PCR)-reverse dot crossing]were compared among all groups.Patients with HPV E6/E7 mRNA expression in the grade 1 CIN group were followed up for 1 yr.The relationship between atypical squamous epithelium and high malignant epithelial neoplasia was investigated by univariate and multivariate analysis.RESULTS The diagnostic sensitivity,specificity,and sensitivity of PCR-reverse point hybrid ization technology for secondary CIN were 70.41%,70.66%,and 0.714,respectively.Sensitivity and specificity for secondary CIN were 752%and 7853%,respectively,the area under the curve value was 0.789.Logistic Multifactorial model analysis revealed that the HPV positive rates and the HPV E6/E7 mRNA positive rates were independent risk factors of CIN grade I(P<0.05).In CIN grade I patients with positive for HPV E6/E7 mRNA,in its orientation to grade CIN patients,in its orientation to grade CIN patients,at 69.2%,compared with patients negative for HPV E6/E7 mRNA(30.8%),significant difference(P<0.05).CONCLUSION HPV E6/E7 mRNA and HPV(PCR-reverse dot hybrid)positive expression have a close relationship with CINgrade disease progression and is an independent risk factor for high-grade CIN lesions.

Factors for the Major Changes in the English Language

本文阐述了引起英语变迁的历史因素-罗马人,条顿人,诺尔曼人的四次入侵;以及音变因素-e音的丢失和元音大转移。此外,印刷业的发展加速了拼写的标准化,十六、十七世纪英国文艺复兴极大地丰富了英语词汇。

Chang'e 5 Undergoing Thermal Test

Chang’e 5 probe has entered its critical phase of AIT.Units,except the lander,are undergoing thermal test.The flight model of Chang’e 5 is composed of an orbiter,a return capsule,a lander and an ascent unit.The thermal tests on the ascent unit,the lander-as-

坦布苏病毒E蛋白结构域Ⅰ/Ⅱ蛋白免疫保护效果

为了探讨原核表达的坦布苏病毒E蛋白结构域Ⅰ/Ⅱ蛋白对于坦布苏病毒感染的免疫保护作用,利用IPTG诱导重组大肠杆菌表达坦布苏病毒E蛋白结构域Ⅰ/Ⅱ蛋白,Anti-FLAG M2 Affinity Gel纯化后进行SDS-PAGE和Western blot鉴定。经SDS-PAGE和Western blot鉴定,纯化后坦布苏病毒E蛋白结构域Ⅰ/Ⅱ蛋白仅有单一目的条带。免疫组小鼠血清ELISA效价均达4.67±0.11(lg10)。攻毒保护试验结果表明,免疫组小鼠体质量在攻毒9 d后高于对照组,荧光定量RT-PCR检测结果显示,攻毒后免疫组小鼠脑、肝脏、脾脏组织中坦布苏病毒核酸含量均低于对照组。表明使用原核表达的坦布苏病毒E蛋白结构域Ⅰ/Ⅱ蛋白免疫可诱导产生高水平抗体,并提供良好的免疫保护力,为开发有效的坦布苏病毒亚单位疫苗提供依据。

eNOS参与铁过载诱导的血管内皮细胞线粒体损伤

目的探讨e NOS及ADMA/DDAHⅡ介导的铁过载对HUVECs细胞线粒体的损伤作用。方法常规培养HUVECs细胞,随机分为正常对照(Ctrl)组、右旋糖酐铁(Iron)组、L-精氨酸(L-Arg)组。48 h后,MTT法检测细胞存活率;HPLC法检测ADMA含量及DDAHⅡ活性;Western blot法检测e NOS表达;比色法检测培养液LDH活性、NO含量、细胞MDA含量以及m PTP开放;流式细胞仪检测心肌细胞ROS含量、线粒体膜电位及细胞凋亡。结果 Iron处理48 h后,HUVECs细胞存活率明显降低,培养液ADMA及LDH活性升高,NO含量减少;细胞e NOS表达下调、DDAHⅡ活性降低;MDA含量与ROS生成明显增加,线粒体膜电位减小,m PTP大量开放,细胞凋亡增加;ADMA生理性对抗剂L-Arg则可明显减弱Iron的上述损伤作用。结论 e NOS参与铁过载诱导的HUVECs细胞线粒体损伤,ADMA/DDAHⅡ机制也可能发挥了作用。