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祛瘀护膜法调控PI3K/Akt通路对反流性食管炎大鼠E-钙黏蛋白表达和食管黏膜修复的影响
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作者 胡绍江 吴婷婷 +5 位作者 朱惠萍 梁国强 丁姮月 杨欣 王敏 孙宏文 《现代中西医结合杂志》 CAS 2024年第15期2066-2077,共12页
目的基于磷脂酰肌醇3-激酶(PI3K)/丝氨酸-苏氨酸蛋白激酶(Akt)信号通路调控E-钙黏蛋白(E-cadherin)的表达,探讨祛瘀护膜法对反流性食管炎(RE)大鼠食管黏膜损伤的影响,进一步明确其作用机制。方法取68只雄性SD大鼠,随机选择10只大鼠作为... 目的基于磷脂酰肌醇3-激酶(PI3K)/丝氨酸-苏氨酸蛋白激酶(Akt)信号通路调控E-钙黏蛋白(E-cadherin)的表达,探讨祛瘀护膜法对反流性食管炎(RE)大鼠食管黏膜损伤的影响,进一步明确其作用机制。方法取68只雄性SD大鼠,随机选择10只大鼠作为假手术组,剩余大鼠采用4.2 mm幽门夹+2/3胃底结扎术方法建立RE模型。将56只造模成功的大鼠随机分为7组,每组8只。雷贝拉唑组给予雷贝拉唑0.0018 g/kg灌胃,铝镁加组给予铝镁加混悬液0.0135 g/kg灌胃,雷贝拉唑+祛瘀护膜剂组给予雷贝拉唑0.0018 g/kg+祛瘀护膜剂1.62 g/kg灌胃,雷贝拉唑+加味祛瘀护膜剂组给予雷贝拉唑0.0018 g/kg+加味祛瘀护膜剂2.97 g/kg灌胃,祛瘀护膜剂组给予祛瘀护膜剂1.62 g/kg灌胃,加味祛瘀护膜剂组给予加味祛瘀护膜剂2.97 g/kg灌胃,假手术组和模型组给予蒸馏水+淀粉灌胃,均1次/d,连续灌胃14 d。观察大鼠一般情况,末次灌胃结束后取出全胃计算胃内色素残留率,HE染色观察食管组织病理形态,透射电镜观察食管组织Cajal间质细胞结构,RT-PCR法检测食管组织中E-cadherin、锌指转录因子(Slug)mRNA表达情况,Western blot法检测食管组织中E-cadherin、半胱氨酸蛋白酶-1(Caspase-1)、磷酸化PI3K(p-PI3K)、磷酸化Akt(p-Akt)、第10号染色体缺失的磷酸酶和张力蛋白同源基因(PTEN)、磷酸肌醇依赖性蛋白激酶-1(PDK1)蛋白表达情况,免疫组织化学法检测食管组织中E-cadherin、Caspase-1、PTEN、PDK1阳性表达情况,ELISA法检测血清中Caspase-1、白细胞介素-1β(IL-1β)、白细胞介素-17(IL-17)、白细胞介素-18(IL-18)、白细胞介素-23(IL-23)水平。结果与假手术组比较,模型组大鼠胃内色素残留率明显升高(P<0.05);食管组织可见炎性细胞浸润,黏膜层轻度增厚,Cajal间质细胞数量减少;食管组织中E-cadherin mRNA相对表达量和E-cadherin、PTEN蛋白相对表达量及E-cadherin、PTEN阳性表达面积率均明显降低(P均<0.05),Slug mRNA相对表达量和Caspase-1、p-PI3K、p-Akt、PDK1蛋白相对表达量及Caspase-1、PDK1阳性表达面积率均明显升高(P均<0.05);血清Caspase-1、IL-1β、IL-17、IL-18、IL-23水平均明显升高(P均<0.05)。与模型组比较,各药物组大鼠胃内色素残留率均明显降低(P均<0.05);食管组织损伤明显减轻;雷贝拉唑+祛瘀护膜剂组、雷贝拉唑+加味祛瘀护膜剂组食管组织中E-cadherin mRNA相对表达量和E-cadherin、PTEN蛋白相对表达量及E-cadherin、PTEN阳性表达面积率均明显升高(P均<0.05),Slug mRNA相对表达量和Caspase-1、p-PI3K、p-Akt、PDK1蛋白相对表达量及Caspase-1、PDK1阳性表达面积率均明显降低(P均<0.05);血清Caspase-1、IL-1β、IL-17、IL-18、IL-23水平均明显降低(P均<0.05)。与雷贝拉唑组比较,雷贝拉唑+祛瘀护膜剂组、雷贝拉唑+加味祛瘀护膜剂组食管组织中E-cadherin mRNA相对表达量和E-cadherin、PTEN蛋白相对表达量及E-cadherin、PTEN阳性表达面积率均明显升高(P均<0.05),Slug mRNA相对表达量和Caspase-1、p-PI3K、p-Akt蛋白相对表达量及Caspase-1、PDK1阳性表达面积率均明显降低(P均<0.05);雷贝拉唑+加味祛瘀护膜剂组食管组织中PDK1蛋白相对表达量和血清Caspase-1、IL-1β、IL-17、IL-18、IL-23水平均明显降低(P均<0.05)。雷贝拉唑+加味祛瘀护膜剂组食管组织中p-PI3K、p-Akt蛋白相对表达量和血清Caspase-1、IL-1β、IL-17、IL-18、IL-23水平均明显低于雷贝拉唑+祛瘀护膜剂组(P均<0.05)。结论祛瘀护膜法可通过抑制PI3K/Akt通路过度激活作用于E-cadherin、Slug转录因子,在下调Caspase-1、p-PI3K、p-Akt、PDK1蛋白表达的同时上调E-cadherin、PTEN蛋白的表达,进而降低血清中Caspase-1、IL-1β、IL-17、IL-18、IL-23水平,减轻上皮组织细胞的炎性病变和(或)抑制细胞的转移、浸润,促进食管黏膜损伤修复,其联合西药治疗效果更佳,且优化组方作用更明显。 展开更多
关键词 反流性食管炎 食管黏膜 祛瘀护膜法 磷脂酰肌醇3-激酶 丝氨酸-苏氨酸蛋白激酶 e-钙黏蛋白
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Metallurgical performance evaluation of space-weathered Chang’e-5 lunar soil
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作者 Chen Li Wenhui Ma +1 位作者 Yang Li Kuixian Wei 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第6期1241-1248,共8页
Space metallurgy is an interdisciplinary field that combines planetary space science and metallurgical engineering.It involves systematic and theoretical engineering technology for utilizing planetary resources in sit... Space metallurgy is an interdisciplinary field that combines planetary space science and metallurgical engineering.It involves systematic and theoretical engineering technology for utilizing planetary resources in situ.However,space metallurgy on the Moon is challenging because the lunar surface has experienced space weathering due to the lack of atmosphere and magnetic field,making the mi-crostructure of lunar soil differ from that of minerals on the Earth.In this study,scanning electron microscopy and transmission electron microscopy analyses were performed on Chang’e-5 powder lunar soil samples.The microstructural characteristics of the lunar soil may drastically change its metallurgical performance.The main special structure of lunar soil minerals include the nanophase iron formed by the impact of micrometeorites,the amorphous layer caused by solar wind injection,and radiation tracks modified by high-energy particle rays inside mineral crystals.The nanophase iron presents a wide distribution,which may have a great impact on the electromagnetic prop-erties of lunar soil.Hydrogen ions injected by solar wind may promote the hydrogen reduction process.The widely distributed amorph-ous layer and impact glass can promote the melting and diffusion process of lunar soil.Therefore,although high-energy events on the lun-ar surface transform the lunar soil,they also increase the chemical activity of the lunar soil.This is a property that earth samples and tradi-tional simulated lunar soil lack.The application of space metallurgy requires comprehensive consideration of the unique physical and chemical properties of lunar soil. 展开更多
关键词 space metallurgy change-5 lunar soil space weathering metallurgical performance
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miR-30e-5p通过靶向PIK3CD介导PI3K/AKT/mTOR信号通路对肝癌细胞侵袭和迁移的影响
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作者 郝云鹤 邹瑞 +1 位作者 赵晓彪 李铎 《中国现代普通外科进展》 CAS 2024年第1期19-24,共6页
目的:探讨mi R-30e-5p通过靶向磷酸肌醇-3-激酶催化亚基δ肽(PIK3CD)介导磷酸肌醇3激酶(PI3K)/蛋白激酶B(AKT)/哺乳动物雷帕霉素靶蛋白(mTOR)信号通路抑制肝癌细胞的侵袭、迁移的机制。方法:将Hep G2通过转染相应质粒后分为对照组、miR-... 目的:探讨mi R-30e-5p通过靶向磷酸肌醇-3-激酶催化亚基δ肽(PIK3CD)介导磷酸肌醇3激酶(PI3K)/蛋白激酶B(AKT)/哺乳动物雷帕霉素靶蛋白(mTOR)信号通路抑制肝癌细胞的侵袭、迁移的机制。方法:将Hep G2通过转染相应质粒后分为对照组、miR-30e-5p mimics组、PIK3CD敲低组、阴性对照组、miR-30e-5p mimics+PIK3CD过表达组,实时荧光定量PCR和免疫印迹检测细胞miR-30e-5p、PIK3CD与PI3K/AKT/mTOR信号通路表达;CCK-8法检测各组细胞增殖率;细胞划痕实验和Transwell侵袭实验分别检测各组细胞迁移、侵袭情况;以免疫印迹实验检测各组细胞基质金属蛋白酶(MMP)2、MMP9及E-cadherin、N-cadherin、Vimentin蛋白表达。采用双荧光素酶报告实验检测miR-30e-5p对PIK3CD的靶向调节。结果:与对照组相比,mi R-30e-5p mimics组、PIK3CD敲低组增殖率、迁移率、侵袭数、PIK3CD蛋白与mRNA表达、N-cadherin、MMP2、MMP9、磷酸化PI3K、磷酸化AKT、磷酸化m TOR蛋白表达降低(P<0.05),E-cadherin表达升高(P<0.05)。过表达PIK3CD减弱了miR-30e-5p mimics对肝癌细胞增殖、迁移、侵袭的抑制作用,并升高PI3K/AKT/mTOR通过相关蛋白表达。miR-30e-5p可靶向下调PIK3CD表达。结论:上调miR-30e-5p可通过降低PIK3CD表达而阻止PI3K/AKT/mTOR信号激活,从而抑制肝癌细胞增殖、迁移和侵袭。 展开更多
关键词 miR-30e-5p PIK3CD PI3K/AKT/MTOR 肝肿瘤 侵袭 迁移
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The formation and evolutionary characteristics of organic matter and pyrites in the continental shales of the 3^(rd)submember of Chang 7 Member,Yanchang formation,Ordos Basin,China
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作者 Ruikang Bian 《Energy Geoscience》 EI 2024年第2期31-39,共9页
Through microscopic analyses(e.g.,organic macerals,thin section observation,scanning electron microscope(SEM)imaging of fresh bedding planes via argon ion milling,and energy spectrum tests)combined with Rock-Eval anal... Through microscopic analyses(e.g.,organic macerals,thin section observation,scanning electron microscope(SEM)imaging of fresh bedding planes via argon ion milling,and energy spectrum tests)combined with Rock-Eval analyses,this study systematically investigated the organic matter and pyrites in the continental shales in the 3^(rd)submember of the Chang 7 Member(Chang 7^(3)submember)in the Yanchang Formation,Ordos Basin and determined their types and the formation and evolutionary characteristics.The results are as follows.The organic matter of the continental shales in the Chang 7^(3)submember is dominated by amorphous bituminites and migrabitumens,which have come into being since the early diagenetic stage and middle diagenetic stage A,respectively.The formation and transformation of organic matter is a prerequisite for the formation of pyrites.The Ordos Basin was a continental freshwater lacustrine basin and lacked sulphates in waters during the deposition of the Chang 7 Member.Therefore,the syndiagenetic stage did not witness the formation of large quantities of pyrites.Since the basin entered early diagenetic stage A,large quantities of sulfur ions were released as the primary organic matter got converted into bituminites and,accordingly,pyrites started to form.However,this stage featured poorer fluid and spatial conditions compared with the syndepositional stage due to withdraw of water,the partial formation of bituminites,and a certain degree of compaction.As a result,large quantities of pyrrhotite failed to transition into typical spherical framboidal pyrites but grew into euhedral monocrystal aggregates.In addition,pyrites are still visible in the migrabitumens in both microfractures and inorganic pores of mudstones and shales,indicating that the pyrite formation period can extend until the middle diagenetic stage A. 展开更多
关键词 Organic matter Pyrite Formation and evolution Continental shale chang 7^(3)submember Ordos Basin
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Lunar Penetrating Radar onboard the Chang'e-3 mission 被引量:15
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作者 Guang-You Fang Bin Zhou +11 位作者 Yi-Cai Ji Qun-Ying Zhang Shao-Xiang Shen Yu-Xi Li Hong-Fei Guan Chuan-Jun Tang Yun-Ze Gao Wei Lu Sheng-Bo Ye Hai-Dong Han Jin Zheng Shu-Zhi Wang 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2014年第12期1607-1622,共16页
Lunar Penetrating Radar (LPR) is one of the important scientific instru- ments onboard the Chang'e-3 spacecraft. Its scientific goals are the mapping of lunar regolith and detection of subsurface geologic structure... Lunar Penetrating Radar (LPR) is one of the important scientific instru- ments onboard the Chang'e-3 spacecraft. Its scientific goals are the mapping of lunar regolith and detection of subsurface geologic structures. This paper describes the goals of the mission, as well as the basic principles, design, composition and achievements of the LPR. Finally, experiments on a glacier and the lunar surface are analyzed. 展开更多
关键词 chang'e-3 mission -- moon rover -- Lunar Penetrating Radar
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Development and calibration of the Moon-based EUV camera for Chang'e-3 被引量:4
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作者 Bo Chen Ke-Fei Song +31 位作者 Zhao-Hui Li Qing-Wen Wu Qi-Liang Ni Xiao-Dong Wang Jin-Jiang Xie Shi-Jie Liu Ling-Ping He Fei He Xiao-Guang Wang Bin Chen Hong-Ji Zhang Xiao-Dong Wang Hai-Feng Wang Xin Zheng Shu-Lin E Yong-Cheng Wang Tao Yu Liang Sun Jin-Ling Wang Zhi Wang Liang Yang Qing-Long Hu Ke Qiao Zhong-Su Wang Xian-Wei Yang Hai-Ming Bao Wen-Guang Liu Zhe Li Ya Chen Yang Gao Hui Sun Wen-Chang Chen 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2014年第12期1654-1663,共10页
The process of development and calibration for the first Moon-based ex- treme ultraviolet (EUV) camera to observe Earth's plasmasphere is introduced and the design, test and calibration results are presented. The E... The process of development and calibration for the first Moon-based ex- treme ultraviolet (EUV) camera to observe Earth's plasmasphere is introduced and the design, test and calibration results are presented. The EUV camera is composed of a multilayer film mirror, a thin film filter, a photon-counting imaging detector, a mech- anism that can adjust the direction in two dimensions, a protective cover, an electronic unit and a thermal control unit. The center wavelength of the EUV camera is 30.2 nm with a bandwidth of 4.6nm. The field of view is 14.7° with an angular resolution of 0.08°, and the sensitivity of the camera is 0.11 count s-1 Rayleigh-1. The geomet- ric calibration, the absolute photometric calibration and the relative photometric cal- ibration are carried out under different temperatures before launch to obtain a matrix that can correct geometric distortion and a matrix for relative photometric correction, which are used for in-orbit correction of the images to ensure their accuracy. 展开更多
关键词 chang'e-3 -- EUV camera: development: calibration -- Earth's plas-masphere -- lunar exploration
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Data processing and preliminary results of the Chang'e-3 VIS/NIR Imaging Spectrometer in-situ analysis 被引量:3
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作者 Bin Liu Chun-Lai Li +7 位作者 Guang-Liang Zhang Rui Xu Jian-Jun Liu Xin Ren Xu Tan Xiao-Xia Zhang Wei Zuo Wei-Bin Wen 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2014年第12期1578-1594,共17页
The Chang'e-3 Visible and Near-infrared Imaging Spectrometer (VNIS) is one of the four payloads on the Yutu rover. After traversing the landing site during the first two lunar days, four different areas are detecte... The Chang'e-3 Visible and Near-infrared Imaging Spectrometer (VNIS) is one of the four payloads on the Yutu rover. After traversing the landing site during the first two lunar days, four different areas are detected, and Level 2A and 2B ra- diance data have been released to the scientific community. The released data have been processed by dark current subtraction, correction for the effect of temperature, radiometric calibration and geometric calibration. We emphasize approaches for re- flectance analysis and mineral identification for in-situ analysis with VNIS. Then the preliminary spectral and mineralogical results from the landing site are derived. After comparing spectral data from VNIS with data collected by the Ma instrument and samples of mare that were returned from the Apollo program, all the reflectance data have been found to have similar absorption features near 1000 nm except lunar sample 71061. In addition, there is also a weak absorption feature between 1750-2400nm on VNIS, but the slopes of VNIS and Ma reflectance at longer wavelengths are lower than data taken from samples of lunar mare. Spectral parameters such as Band Centers and Integrated Band Depth Ratios are used to analyze mineralogical features. The results show that detection points E and N205 are mixtures of high-Ca pyroxene and olivine, and the composition of olivineat point N205 is higher than that at point E, but the compositions of detection points S3 and N203 are mainly olivine-rich. Since there are no obvious absorption features near 1250 nm, plagioclase is not directly identified at the landing site. 展开更多
关键词 chang'e-3 -- VNIS -- in-situ analysis -- data processing
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黄芩素对人乳腺癌细胞系MDA-MB-231侵袭、迁移、上皮间充质转化的调控作用及其机制
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作者 陈林 梁秋果 +1 位作者 吉杨丹 王恒 《山东医药》 CAS 2024年第6期10-13,共4页
目的观察黄岑素对人乳腺癌细胞系MDA-MB-231的侵袭、迁移及上皮间充质转化(EMT)的调控作用,探讨其可能作用机制。方法取对数生长期MDA-MB-231细胞分为一组、二组、三组及对照组,一组、二组、三组分别加入2.5、5、10μmol/L的黄岑素,对... 目的观察黄岑素对人乳腺癌细胞系MDA-MB-231的侵袭、迁移及上皮间充质转化(EMT)的调控作用,探讨其可能作用机制。方法取对数生长期MDA-MB-231细胞分为一组、二组、三组及对照组,一组、二组、三组分别加入2.5、5、10μmol/L的黄岑素,对照组不做任何处理。培养48 h时采用划痕修复实验观察四组细胞迁移能力、采用Transwell侵袭实验观察四组细胞侵袭能力,采用Western Blotting法检测细胞EMT标志物波形蛋白(vimentin)及E-钙黏蛋白(E-cadherin)、整合素αv、β3、磷酸化黏着斑激酶(p-FAK)、磷酸化磷脂酰肌醇3激酶(整合素p-PI3K)。结果与对照组相比,黄岑素组细胞迁移率降低、侵袭细胞数少,细胞E-cadherin相对表达量高,vimentin、整合素αv、整合素β3、p-FAK、p-PI3K蛋白相对表达量低,且呈剂量依赖性(P均<0.05)。结论黄芩素抑制MDA-MB-231细胞的侵袭、迁移及EMT。黄岑素可能通过抑制整合素αv、整合素β3表达,进一步抑制p-FAK、p-PI3K蛋白表达,抑制MDA-MB-231的侵袭、迁移及EMT。 展开更多
关键词 黄芩素 乳腺癌 细胞侵袭 细胞迁移 上皮间质转化 波形蛋白 e-钙黏蛋白 整合素αv、整合素β3 黏着斑激酶 磷脂酰肌醇3激酶
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Preface: The Chang'e-3 lander and rover mission to the Moon 被引量:1
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作者 Wing-Huen Ip Jun Yan +1 位作者 Chun-Lai Li Zi-Yuan Ouyang 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2014年第12期1511-1513,共3页
The Chang'e-3 (CE-3) lander and rover mission to the Moon was an in- termediate step in China's lunar exploration program, which will be followed by a sample return mission. The lander was equipped with a number o... The Chang'e-3 (CE-3) lander and rover mission to the Moon was an in- termediate step in China's lunar exploration program, which will be followed by a sample return mission. The lander was equipped with a number of remote-sensing instruments including a pair of cameras (Landing Camera and Terrain Camera) for recording the landing process and surveying terrain, an extreme ultraviolet camera for monitoring activities in the Earth's plasmasphere, and a first-ever Moon-based ultravi- olet telescope for astronomical observations. The Yutu rover successfully carried out close-up observations with the Panoramic Camera, mineralogical investigations with the VIS-NIR Imaging Spectrometer, study of elemental abundances with the Active Particle-induced X-ray Spectrometer, and pioneering measurements of the lunar sub- surface with Lunar Penetrating Radar. This special issue provides a collection of key information on the instrumental designs, calibration methods and data processing pro- cedures used by these experiments with a perspective of facilitating further analyses of scientific data from CE-3 in preparation for future missions. 展开更多
关键词 chang'e-3 - Moon lander - rover - radar - lunar telescope
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Correlations between plasmapause evolutions and auroral signatures during substorms observed by Chang'e-3 EUV Camera 被引量:2
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作者 XiaoXin Zhang Fei He +2 位作者 Bo Chen Chao Shen HuaNing Wang 《Earth and Planetary Physics》 2017年第1期35-43,共9页
The plasmapause locations determined from the Chang'e-3(CE-3) Extreme Ultraviolet Camera(EUVC) images and the auroral boundaries determined from the Defense Meteorological Satellite Program(DMSP) Special Sensor Ul... The plasmapause locations determined from the Chang'e-3(CE-3) Extreme Ultraviolet Camera(EUVC) images and the auroral boundaries determined from the Defense Meteorological Satellite Program(DMSP) Special Sensor Ultraviolet Spectrographic Imager(SSUSI) images are used to investigate the plasmaspheric evolutions during substorms. The most important finding is a nightside pointing plasmaspheric plume observed at 23:05 UT on 21 April 2014 under quiet solar wind and geomagnetic conditions, which drifted from the dusk sector. High correlations between the plasmapause evolutions and the auroral signatures exist during substorms. After substorm onset, the plasmapause erosion and the equatorward expansion of the auroral oval occur almost simultaneously in both MLT and UT, and then both the erosion and the expansion propagate westward and eastward. It is suggested that the plasmaspheric erosion and its MLT propagations are induced by the enhanced earthward plasma convection during substorm period, and the substorm dipolarization causes pitch-angle scattering of plasma sheet electrons and the resulting precipitation excites aurora emissions at the same time. 展开更多
关键词 PLASMASPHERE change-3 EUVC minimum L Algorithm SUBSTORM
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Deployment Reliability Test and Assessment for Landing Gear of Chang' E-3 Probe 被引量:1
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作者 吴琼 杨建中 +2 位作者 傅惠民 徐青华 满剑锋 《Journal of Donghua University(English Edition)》 EI CAS 2014年第6期782-784,800,共4页
Chang'E-3 probe incorporates four landing gears to assure the soft-landing,which are stowed while launching and deployed after separated from rocket. Deployment reliability is quite crucial for this mission. The d... Chang'E-3 probe incorporates four landing gears to assure the soft-landing,which are stowed while launching and deployed after separated from rocket. Deployment reliability is quite crucial for this mission. The deployment reliability test( DRT) method and assessment method were developed in this paper. Then DRT was conducted and the deployment reliability estimate of Chang'E-3probe was used to verify the proposed methods. 展开更多
关键词 change-3 landing gear DEPLOYMENT RELIABILITY test ASSESSMENT
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自然杀伤细胞来源外泌体的miR-30e-3p抑制人食管鳞状细胞癌细胞增殖和侵袭
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作者 孙明月 李洪霖 冯保荣 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2023年第4期295-302,共8页
目的 研究自然杀伤(NK)细胞来源外泌体的miR-30e-3p对食管鳞状细胞癌(ESCC)细胞增殖、凋亡和侵袭的影响。方法 从健康供体的外周血中分离和扩增NK细胞,并分离NK细胞外泌体(EXO)进行鉴定。NK细胞外泌体与NEC细胞进行共培养,随机分为Exo1... 目的 研究自然杀伤(NK)细胞来源外泌体的miR-30e-3p对食管鳞状细胞癌(ESCC)细胞增殖、凋亡和侵袭的影响。方法 从健康供体的外周血中分离和扩增NK细胞,并分离NK细胞外泌体(EXO)进行鉴定。NK细胞外泌体与NEC细胞进行共培养,随机分为Exo1和Exo2组。采用透射电子显微镜观察外泌体形态及大小。Western blot法检测外泌体凋亡相关基因2相互作用蛋白X(ALIX)、抗肿瘤易感基因101(TSG101)、 CD81、钙联蛋白(calnexin)表达。将miR-30e-3p模拟物(miR-30e-3p mimic)、 miR-30e-3p抑制物(miR-30e-3p inhibitor)及阴性对照(miR-NC)转入NK细胞,并分离相应的外泌体与NEC细胞进行共培养。CCK-8法检测细胞增殖,流式细胞术检测细胞周期,异硫氰酸荧光素标记的膜联素Ⅴ/碘化丙啶双染法检测细胞凋亡,Transwell^(TM)侵袭实验检测细胞侵袭能力。实时定量PCR检测NCE细胞和外泌体中miR-23b、 miR-422a、 miR-133b、 miR-124、 miR-30e-3p和miR-99a的表达。结果 NK细胞中CD56+CD3+细胞比例为(0.071±0.008)%,CD56+CD16+细胞比例为(90.6±10.6)%。从NK细胞分离的外泌体直径为30~150 nm,且表达ALIX、 TSG101、 CD81,不表达calnexin。NK细胞来源外泌体抑制NEC细胞增殖和侵袭、增加细胞凋亡,降低S期细胞比例并增加G1期细胞比例。过表达miR-30e-3p的NK细胞来源外泌体抑制NEC细胞增殖和侵袭,并阻滞细胞周期和促进细胞凋亡,敲低miR-30e-3p的NK细胞来源外泌体则相反。结论NK细胞来源外泌体miR-30e-3p阻断人ESCC细胞的细胞周期,抑制其增殖和侵袭并诱导其凋亡。 展开更多
关键词 食管鳞状细胞癌 自然杀伤(NK)细胞 外泌体 miR-30e-3p
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Visualization of Landscape Changes in a 3D Environment Using the Storytelling Approach—The Example of the City of Pristina
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作者 Festina Sadiku 《Journal of Geographic Information System》 2023年第5期482-514,共33页
This study proposes a detailed concept of how landscape changes can be transferred and communicated in a 3D environment using the storytelling approach. In 2018, Mocnik and Fairbairn argued that maps are good for repr... This study proposes a detailed concept of how landscape changes can be transferred and communicated in a 3D environment using the storytelling approach. In 2018, Mocnik and Fairbairn argued that maps are good for representing a geographic space but texts have a better benefit than maps for telling a story [1]. A framework is presented on how a landscape change can be retrieved out of textual descriptions. A prototype of a 3D model with a projection on top of it was developed. The case study tells the story of the landscape change in the city of Pristina. The key element of the visualization is a timeline. Several media like cadastral maps, orthophotos, texts, graphics, and background sound are used and combined in an animated light show and the visualization was evaluated within a user study. 展开更多
关键词 Landscape change VISUALIZATION STORYTELLING 3D Models Pristina
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基于机器学习的汾渭平原PM_(2.5)和O_(3)变化特征及影响因素
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作者 李焕 苏慧 +2 位作者 张婷 赵竹子 王璐瑶 《环境化学》 CAS CSCD 北大核心 2024年第5期1585-1598,共14页
本文以2017—2021年汾渭平原典型城市西安大气PM_(2.5)和O_(3)浓度数据为基础,运用机器学习方法分析了PM_(2.5)和O_(3)的变化特征和趋势,讨论了污染气体(NO_(2)、SO_(2)、CO和HCHO)与气象因素(温度、相对湿度、风速、大气压力、边界层... 本文以2017—2021年汾渭平原典型城市西安大气PM_(2.5)和O_(3)浓度数据为基础,运用机器学习方法分析了PM_(2.5)和O_(3)的变化特征和趋势,讨论了污染气体(NO_(2)、SO_(2)、CO和HCHO)与气象因素(温度、相对湿度、风速、大气压力、边界层高度和太阳辐射)对PM_(2.5)和O_(3)浓度变化的交互影响.Theil-Sen趋势分析发现2017—2021年西安市PM_(2.5)和O_(3)分别以每年6.03%和2.06%的速度下降.单因素广义加性模型(GAM)中,NO_(2)、SO_(2)和CO对PM_(2.5)浓度变化影响的模型解释率较高,温度、太阳辐射和大气压对O_(3)浓度变化影响的模型解释率较高.多因素GAM模型中,PM_(2.5)和O_(3)均呈现非线性变化,模型的解释方差分别为84.9%和75.0%,拟合程度较高.通过等高线图分析了多个气象因素和多种污染气体两两交互作用分别对PM_(2.5)和O_(3)浓度的影响,其中温度和污染气体(NO_(2)、SO_(2)、CO和O_(3))对PM_(2.5)浓度的影响更大;温度和太阳辐射对O_(3)的影响更大.NO_(2)和CO分别与气象条件两两相互作用时,PM_(2.5)浓度随NO_(2)和CO的增高而增高,O_(3)浓度则与NO_(2)和CO的变化趋势相反.结合本地污染物源清单,建议加强控制工业源和移动源排放,有助于降低PM_(2.5)和O_(3)的浓度. 展开更多
关键词 PM2.5 O_(3) 机器学习 变化趋势 影响因素
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Nomenclature for lunar features at the Chang'e-3 landing site
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作者 Zhoubin Zhang Chunlai Li +1 位作者 Wei Zuo Xingguo Zeng 《Acta Geochimica》 EI CAS CSCD 2017年第2期213-223,共11页
Nomenclatures for lunar features always accompany the progresses of human lunar exploration,which has an important dual meaning in culture and science. The naming of lunar features not only can commemorate the outstan... Nomenclatures for lunar features always accompany the progresses of human lunar exploration,which has an important dual meaning in culture and science. The naming of lunar features not only can commemorate the outstanding contributions of academics,masters in various fields, and popularize the traditional cultures of ethnic groups all over the world, but also have a critical function of providing accurate indicative information on features with special morphology, origin, nature and scientific value. However, nomenclature for features at the Chang'e-3 landing site, which has a more arbitrary form without many constrains posed by a uniformed system, is unlike the features for other morphological units.This paper originated from the actual needs for the description of scientific exploration activities, interpretation of scientific research and dissemination of scientific results. Some prominent morphological units with great scientific importance and identification purpose were chosen from the images taken by the terrain camera, panorama cameras and landing camera onboard the Chang'e lander and Yutu rover. A nomenclature system was established under the three enclosures, four quadrants and twenty-eight lunar lodges' system of the Chinese ancient sky division method. Finally, a standard feature names set waspublished after some necessary approval procedures by the International Astronomical Union. 展开更多
关键词 MOON chang'e-3 Landing site Lunarfeature nomenclature
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Na_(2)CO_(3)·10H_(2)O-Na_(2)HPO_(4)·12H_(2)O/SiO_(2)复合定形相变材料的制备及应用 被引量:1
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作者 王璇 李再超 +2 位作者 吴亚楠 唐炳涛 张宇昂 《精细化工》 EI CAS CSCD 北大核心 2024年第3期623-629,656,共8页
以Na_(2)CO_(3)·10H_(2)O(SCD)、Na_(2)HPO_(4)·12H_(2)O(DHPD)为相变主体制备了共晶体系,通过绘制凝固点变化图与DSC测试共同确定在m(SCD)∶m(DHPD)=4∶6时形成共晶,FTIR和XRD结果显示,2种水合盐间没有发生化学反应,但其晶... 以Na_(2)CO_(3)·10H_(2)O(SCD)、Na_(2)HPO_(4)·12H_(2)O(DHPD)为相变主体制备了共晶体系,通过绘制凝固点变化图与DSC测试共同确定在m(SCD)∶m(DHPD)=4∶6时形成共晶,FTIR和XRD结果显示,2种水合盐间没有发生化学反应,但其晶型结构发生改变。通过添加质量分数为2%的Na2SiO3·9H_(2)O作为成核剂降低体系的过冷度,且经历50次相变循环体系未出现相分离,相变焓值仅下降0.25%。进一步使用质量分数为25%的气相SiO_(2)作为支撑材料,采用浸渍法制备了相变前后形状稳定的共晶水合盐/SiO_(2)定形相变材料(SSPCM)。所得SSPCM的相变温度为24.08℃,相变焓值为146.6J/g,过冷度为0.55℃,热导率为0.4571W/(m·K)。同保温泡沫相比,其可将模拟房内部中心温度的升温时间延长了1.81倍,降温时间延长了0.39倍。 展开更多
关键词 Na_(2)CO_(3)·10H_(2)O Na_(2)HPO_(4)·12H_(2)O 共晶水合盐 复合相变材料 建筑节能 功能材料
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Organic matter transformation ratio, hydrocarbon expulsion efficiency and shale oil enrichment type in Chang 7_(3) shale of Upper Triassic Yanchang Formation in Ordos Basin, NW China
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作者 ZHAO Wenzhi BIAN Congsheng +4 位作者 LI Yongxin LIU Wei DONG Jin WANG Kun ZENG Xu 《Petroleum Exploration and Development》 2023年第1期14-26,共13页
The major enrichment type of shale oil in the Chang 7_(3) shale of Upper Triassic Yanchang Formation in the Ordos Basin is unknown.This paper analyzes the organic matter transformation ratio,hydrocarbon expulsion effi... The major enrichment type of shale oil in the Chang 7_(3) shale of Upper Triassic Yanchang Formation in the Ordos Basin is unknown.This paper analyzes the organic matter transformation ratio,hydrocarbon expulsion efficiency and roof/floor sealing conditions of the Chang 7_(3) shale,and evaluates the major enrichment type of shale oil in this interval.The average organic matter transformation ratio of the Chang 7_(3) shale is about 45%;in other words,more than 50%of the organic matters have not transformed to hydrocarbons,and the lower the maturity,the greater the proportion of untransformed organic matters.The cumulative hydrocarbon expulsion efficiency of the transformed hydrocarbon is 27.5% on average,and the total proportion of untransformed organic matters plus retained hydrocarbons is greater than 70%.The relative hydrocarbon expulsion efficiency of the Chang 7_(3) shale is 60%on average,that is,about 40% of hydrocarbons retain in the shale.The Chang 7_(3) shale corresponds to Chang 7_(1+2) and Chang 8 sandstones as the roof and floor,respectively,and is further overlaid by Chang 6 shale,where extensive low porosity and low permeability–tight oil reservoirs have formed in the parts with relatively good porosity and permeability.Moreover,the Chang 7_(3) shale is tested to be in a negative pressure system(the pressure coefficient of 0.80–0.85).Therefore,the roof/floor sealing conditions of the Chang 7_(3) shale are poor.The retained hydrocarbons appear mostly in absorbed status,with low mobility.It is concluded that the medium–high mature shale oil is not the major enrichment type of shale oil in the Chang 7_(3) shale,but there may be enrichment opportunity for shale oil with good mobility in the areas where the sealing conditions are good without faults and fractures and oil reservoirs are formed off Chang 7_(1+2),Chang 6 and Chang 8.Furthermore,low–medium mature shale oil is believed to have great potential and is the major enrichment type of shale oil in the Chang 7_(3) shale.It is recommended to prepare relevant in-situ conversion technologies by pilot test and figure out the resource availability and distribution. 展开更多
关键词 organic matter transformation ratio hydrocarbon expulsion efficiency sealing condition continental shale oil major enrichment type chang 7_(3)shale Triassic Ordos Basin
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太阳盐基@Ti_(3)C_(2)T_(x)相变材料的制备与性能评价
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作者 张浩楠 刘汉涛 《当代化工》 CAS 2024年第8期1855-1859,共5页
以硝酸钾、硝酸钠组成的二元硝酸盐为基盐,采用熔融共混法制备了太阳盐@Ti_(3)C_(2)T_(x)复合相变材料,通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)对复合相变材料的微观形貌、结晶结构进行了表征,以X射线光电子能谱(XPS)来分析材料... 以硝酸钾、硝酸钠组成的二元硝酸盐为基盐,采用熔融共混法制备了太阳盐@Ti_(3)C_(2)T_(x)复合相变材料,通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)对复合相变材料的微观形貌、结晶结构进行了表征,以X射线光电子能谱(XPS)来分析材料的物相及晶体结构,利用差示扫描量热仪(DSC)对复合相变材料的熔点、凝固温度以及比热容进行了测量,利用热常数分析仪进行了导热系数的测量。结果表明:Ti_(3)C_(2)T_(x)可以均匀地分散到太阳盐的内部结构中,当Ti_(3)C_(2)T_(x)纳米片质量分数为1.5%时,熔盐纳米流体的比热容可以达到2.13 J·g^(-1)·K^(-1),提高了47%,提高了整体的相变储热材料的蓄热能力;室温下热导率可以达到1.3205 W·m^(-1)·K^(-1),提高了37%,可提高相变材料的充放热效率。 展开更多
关键词 Ti_(3)C_(2)T_(x) 太阳盐 相变 热传导 太阳能 纳米材料
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An Improved Coupled Level Set and Continuous Moment-of-Fluid Method for Simulating Multiphase Flows with Phase Change
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作者 Zhouteng Ye Cody Estebe +8 位作者 Yang Liu Mehdi Vahab Zeyu Huang Mark Sussman Alireza Moradikazerouni Kourosh Shoele Yongsheng Lian Mitsuhiro Ohta M.Yousuff Hussaini 《Communications on Applied Mathematics and Computation》 EI 2024年第2期1034-1069,共36页
An improved algorithm for computing multiphase flows is presented in which the multimaterial Moment-of-Fluid(MOF)algorithm for multiphase flows,initially described by Li et al.(2015),is enhanced addressing existing MO... An improved algorithm for computing multiphase flows is presented in which the multimaterial Moment-of-Fluid(MOF)algorithm for multiphase flows,initially described by Li et al.(2015),is enhanced addressing existing MOF difficulties in computing solutions to problems in which surface tension forces are crucial for understanding salient flow mechanisms.The Continuous MOF(CMOF)method is motivated in this article.The CMOF reconstruction method inherently removes the"checkerboard instability"that persists when using the MOF method on surface tension driven multiphase(multimaterial)flows.The CMOF reconstruction algorithm is accelerated by coupling the CMOF method to the level set method and coupling the CMOF method to a decision tree machine learning(ML)algorithm.Multiphase flow examples are shown in the two-dimensional(2D),three-dimensional(3D)axisymmetric"RZ",and 3D coordinate systems.Examples include two material and three material multiphase flows:bubble formation,the impingement of a liquid jet on a gas bubble in a cryogenic fuel tank,freezing,and liquid lens dynamics. 展开更多
关键词 Moment-of-Fluid(MOF) Surface tension Two phase flow Phase change Deforming boundaries with change(s)in topology Two-dimensional(2D) Three-dimensional(3D)axisymmetric 3D
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Reliability Design for the Landing Gear of Chang'E-3 Probe
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作者 杨建中 吴琼 +1 位作者 满剑锋 朱汪 《Journal of Donghua University(English Edition)》 EI CAS 2015年第6期1006-1010,共5页
Landing gear is one of the important components of Chang'E-3 Probe.The device was used to absorb the impact energy of Chang'E-3 Probe during the landing process.After landing on the moon,it can support the lan... Landing gear is one of the important components of Chang'E-3 Probe.The device was used to absorb the impact energy of Chang'E-3 Probe during the landing process.After landing on the moon,it can support the lander steadily for a long time.In order to assure the reliability of the landing gear and deal with the extremely severe landing conditions,many reliability measures were adopted in the design procedure,such as selection of cushion materials,optimization of mechanism configuration,design of deployment mode,allocation of buffer force and control of its variation.The successful landing on the moon of Chang'E-3 Probe has completely verified the high reliability of the landing gear. 展开更多
关键词 landing gear change-3 Probe RELIABILITY design material CONFIGURATION
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