目的研究ATP结合盒亚家族A成员8(ABCA8)在结直肠癌中的作用。方法下载癌症基因组图谱数据库(The Cancer Genome Atlas,TCGA)中的结直肠癌数据,利用limma R包研究ABCA8在TCGA数据集中的表达情况。随即利用结直肠癌的临床生存状态数据研...目的研究ATP结合盒亚家族A成员8(ABCA8)在结直肠癌中的作用。方法下载癌症基因组图谱数据库(The Cancer Genome Atlas,TCGA)中的结直肠癌数据,利用limma R包研究ABCA8在TCGA数据集中的表达情况。随即利用结直肠癌的临床生存状态数据研究该基因是否和结直肠癌的生存预后相关。利用基因相关性分析研究ABCA8在结直肠癌中和其他基因之间是否具有相关性。利用R包estimate包和CIBERSORT R包得到结直肠癌中每个样本的肿瘤微环境打分以及运行肿瘤免疫浸润分析,并且对ABCA8基因表达量高低的两个分组之间进行免疫细胞以及肿瘤微环境的表达进行分析。利用免疫检查点相关基因分析ABCA8和其之间的关联。采用Western Blot验证收集的结直肠癌患者癌、癌旁以及正常组织中的ABCA8的表达情况,并且同时验证人类蛋白图谱数据库(The Human Protein Atlas,HPA)中ABCA8基因在结直肠癌以及正常肠道组织中的表达水平变化。结果通过差异分析发现ABCA8在结直肠癌中低表达。通过相关性分析一共得到了多个与ABCA8密切相关的基因。通过肿瘤微环境评分发现依据ABCA8表达水平不同分成的高、低表达两组中,基质评分,免疫评分和肿瘤微环境评分均有差异。利用生物信息学分析还发现ABCA8基因和免疫细胞以及免疫检查点相关基因之间具有明显的关联。通过Western Blot和HPA数据库中发现正常肠组织中ABCA8的表达水平高于结直肠癌组织。结论ABCA8在结直肠癌和正常肠道组织中具有明显差异,可以作为一种新的诊断生物标志物。展开更多
Based on the Late Paleozoic geological background and the latest exploration achievements of the Ordos Basin and North China platform, it is concluded that during the sedimentary period of Permian He 8 Member, the are...Based on the Late Paleozoic geological background and the latest exploration achievements of the Ordos Basin and North China platform, it is concluded that during the sedimentary period of Permian He 8 Member, the area in concern had multiple material sources, multiple river systems, flat terrain, shallow sedimentary water, widely distributed fluvial facies sand body and no continuous lake area, so alluvial river sedimentary system developed in the whole region. Based on stratigraphic correlation and division, and a large number of drilling and outcrop data, a comprehensive analysis of lithofacies and sedimentary facies types and distribution was carried out to reconstruct the ancient geographic pattern of the He 8 Member sedimentary period. The results of paleogeography restoration show that the area of Ordos Basin was the "runoff area" in the sedimentary slope in the western part of the North China platform during the sedimentary period of He 8 Member, the whole region was mainly alluvial plain sedimentation featuring alternate fluvial facies, flood plain facies and flood-plain lake facies. According to the results of flume deposition simulation experiment, a new sedimentary model of "alluvial river & flood-plain lake" is established, which reveals the genesis of large area gravel sand body in He 8 Member of this area and provides geological basis for the exploration of tight gas in the south of the basin.展开更多
文摘目的研究ATP结合盒亚家族A成员8(ABCA8)在结直肠癌中的作用。方法下载癌症基因组图谱数据库(The Cancer Genome Atlas,TCGA)中的结直肠癌数据,利用limma R包研究ABCA8在TCGA数据集中的表达情况。随即利用结直肠癌的临床生存状态数据研究该基因是否和结直肠癌的生存预后相关。利用基因相关性分析研究ABCA8在结直肠癌中和其他基因之间是否具有相关性。利用R包estimate包和CIBERSORT R包得到结直肠癌中每个样本的肿瘤微环境打分以及运行肿瘤免疫浸润分析,并且对ABCA8基因表达量高低的两个分组之间进行免疫细胞以及肿瘤微环境的表达进行分析。利用免疫检查点相关基因分析ABCA8和其之间的关联。采用Western Blot验证收集的结直肠癌患者癌、癌旁以及正常组织中的ABCA8的表达情况,并且同时验证人类蛋白图谱数据库(The Human Protein Atlas,HPA)中ABCA8基因在结直肠癌以及正常肠道组织中的表达水平变化。结果通过差异分析发现ABCA8在结直肠癌中低表达。通过相关性分析一共得到了多个与ABCA8密切相关的基因。通过肿瘤微环境评分发现依据ABCA8表达水平不同分成的高、低表达两组中,基质评分,免疫评分和肿瘤微环境评分均有差异。利用生物信息学分析还发现ABCA8基因和免疫细胞以及免疫检查点相关基因之间具有明显的关联。通过Western Blot和HPA数据库中发现正常肠组织中ABCA8的表达水平高于结直肠癌组织。结论ABCA8在结直肠癌和正常肠道组织中具有明显差异,可以作为一种新的诊断生物标志物。
基金Supported by the China National Science and Technology Major Project(2011ZX05044,2011ZX05007-004)
文摘Based on the Late Paleozoic geological background and the latest exploration achievements of the Ordos Basin and North China platform, it is concluded that during the sedimentary period of Permian He 8 Member, the area in concern had multiple material sources, multiple river systems, flat terrain, shallow sedimentary water, widely distributed fluvial facies sand body and no continuous lake area, so alluvial river sedimentary system developed in the whole region. Based on stratigraphic correlation and division, and a large number of drilling and outcrop data, a comprehensive analysis of lithofacies and sedimentary facies types and distribution was carried out to reconstruct the ancient geographic pattern of the He 8 Member sedimentary period. The results of paleogeography restoration show that the area of Ordos Basin was the "runoff area" in the sedimentary slope in the western part of the North China platform during the sedimentary period of He 8 Member, the whole region was mainly alluvial plain sedimentation featuring alternate fluvial facies, flood plain facies and flood-plain lake facies. According to the results of flume deposition simulation experiment, a new sedimentary model of "alluvial river & flood-plain lake" is established, which reveals the genesis of large area gravel sand body in He 8 Member of this area and provides geological basis for the exploration of tight gas in the south of the basin.
