The spatial distribution of ammonia-oxidizing Betaproteobacteria （βAOB） was investigated by FISH （fluorescence in situ hybridization） and DGGE （denaturing gradient get electrophoresis） techniques in the sedimen...The spatial distribution of ammonia-oxidizing Betaproteobacteria （βAOB） was investigated by FISH （fluorescence in situ hybridization） and DGGE （denaturing gradient get electrophoresis） techniques in the sediment off the Changjiang River Estuary. Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006. The abundance of βAOB ranged from 1.87× 10^5 to 3.53×10^5 cells/g of sediment. βAOB abundance did not present a negative correlation with salinity, whereas salinity was implicated as the primary factor affecting nitrification rates. The DGGE profiles of PCR-amplified amoA gene fragments revealed that the βAOB community structure of sample S2 separated from other samples at the level of 40% similarity. The variations in composition ofβAOB were significantly correlated with the salinity, temperature, absorption ability of sediments and TOC. The statistical analysis indicates that theβAOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity. Considering the good correlation between βAOB abundance and nitrification estimates, the abundance and diversity of βAOB community could be expected as an indirect index of nitrification activity at the study sea area in summer.展开更多
This study investigated the impact of soil texture and sweetpotato cropping system on soil erosion and nutrient loss in the drought infield of the Three Gorges Reservoir Area of Changjiang River under field conditions...This study investigated the impact of soil texture and sweetpotato cropping system on soil erosion and nutrient loss in the drought infield of the Three Gorges Reservoir Area of Changjiang River under field conditions. A factorial experiment was conducted in the study using five soil textures and two cropping systems. The lost soil during the crop season was recovered by a soil-blocking device and the dry weights for the total lost soil and its nutrient components, such as ammonium nitrogen, effective phosphorus, K^＋ and organic matter were analyzed. We found that soil texture significantly affected the dry weights of the total lost soil, effective phosphorus, K^＋, and organic matter, while sweetpotato cropping systems and interaction between soil texture and sweetpotato cropping system affected the dry weights of the total lost soil, the effective phosphorus and organic matter. Among the five soil textures tested, Da and Huang caused significantly less soil erosion and nutrient loss compared with the other three soil textures; intercropping sweetpotato with corn significantly reduced soil erosion and nutrient loss.展开更多
基金The National Fundamental Project of China under grant No.2006CB400602
文摘The spatial distribution of ammonia-oxidizing Betaproteobacteria （βAOB） was investigated by FISH （fluorescence in situ hybridization） and DGGE （denaturing gradient get electrophoresis） techniques in the sediment off the Changjiang River Estuary. Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006. The abundance of βAOB ranged from 1.87× 10^5 to 3.53×10^5 cells/g of sediment. βAOB abundance did not present a negative correlation with salinity, whereas salinity was implicated as the primary factor affecting nitrification rates. The DGGE profiles of PCR-amplified amoA gene fragments revealed that the βAOB community structure of sample S2 separated from other samples at the level of 40% similarity. The variations in composition ofβAOB were significantly correlated with the salinity, temperature, absorption ability of sediments and TOC. The statistical analysis indicates that theβAOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity. Considering the good correlation between βAOB abundance and nitrification estimates, the abundance and diversity of βAOB community could be expected as an indirect index of nitrification activity at the study sea area in summer.
文摘This study investigated the impact of soil texture and sweetpotato cropping system on soil erosion and nutrient loss in the drought infield of the Three Gorges Reservoir Area of Changjiang River under field conditions. A factorial experiment was conducted in the study using five soil textures and two cropping systems. The lost soil during the crop season was recovered by a soil-blocking device and the dry weights for the total lost soil and its nutrient components, such as ammonium nitrogen, effective phosphorus, K^＋ and organic matter were analyzed. We found that soil texture significantly affected the dry weights of the total lost soil, effective phosphorus, K^＋, and organic matter, while sweetpotato cropping systems and interaction between soil texture and sweetpotato cropping system affected the dry weights of the total lost soil, the effective phosphorus and organic matter. Among the five soil textures tested, Da and Huang caused significantly less soil erosion and nutrient loss compared with the other three soil textures; intercropping sweetpotato with corn significantly reduced soil erosion and nutrient loss.
