[Objectives]This study was conducted to establish TLC methods for Scutellariae Radix,Peucedani Radix and Puerariae Radix in Chaige Changyuan Mixture.[Methods]The preparation methods of test solutions,the development c...[Objectives]This study was conducted to establish TLC methods for Scutellariae Radix,Peucedani Radix and Puerariae Radix in Chaige Changyuan Mixture.[Methods]The preparation methods of test solutions,the development conditions,the color development inspection method and other factors were investigated for thin-layer chromatography.The three main medicines of Scutellariae Radix,Peucedani Radix and Puerariae Radix were qualitatively identified.[Results]The TLC spots of Scutellariae Radix,Peucedani Radix and Puerariae Radix could be identified well by TLC,and corresponding negative control had no interference.[Conclusions]The method is simple,available and reproducible,and can be used for the quality control of Chaige Changyuan Mixture.展开更多
基金Supported by First-class Discipline Construction Project in Guizhou Province(Chinese Pharmacy)(GNYL[2017]008):Subsidiary Subject(GNYL[2017]008-7)Scientific Research Project of Guizhou University of Traditional Chinese Medicine(3040-04020001406)。
文摘[Objectives]This study was conducted to establish TLC methods for Scutellariae Radix,Peucedani Radix and Puerariae Radix in Chaige Changyuan Mixture.[Methods]The preparation methods of test solutions,the development conditions,the color development inspection method and other factors were investigated for thin-layer chromatography.The three main medicines of Scutellariae Radix,Peucedani Radix and Puerariae Radix were qualitatively identified.[Results]The TLC spots of Scutellariae Radix,Peucedani Radix and Puerariae Radix could be identified well by TLC,and corresponding negative control had no interference.[Conclusions]The method is simple,available and reproducible,and can be used for the quality control of Chaige Changyuan Mixture.