期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
Application value of ATP based bioluminescence tumor chemosensitivity assay in the chemotherapy for hydrothorax caused by non-small cell lung cancer 被引量:1
1
作者 Kaijian Le Yuming Jia +1 位作者 Jing Wang Maoqiong Jiang 《The Chinese-German Journal of Clinical Oncology》 CAS 2013年第5期210-212,共3页
Objective: The aim of the study was to investigate the clinical value and application of ATP based bioluminescence tumor chemosensitivity assay (ATP-TCA) in the chemotherapy for hydrothorax caused by non-small cell... Objective: The aim of the study was to investigate the clinical value and application of ATP based bioluminescence tumor chemosensitivity assay (ATP-TCA) in the chemotherapy for hydrothorax caused by non-small cell lung cancer (NSCLC). Methods: Hydrothorax specimens from 120 NSCLC patients were analyzed by ATP-TCA and the most sensitive chemotherapeutic drugs were used in NSCLC patients (treatment group). At the same time, 56 NSCLC patients with hydrethorax were admitted in our Hospital (Department of Oncology, The No. 2 People's Hospital of Yibin, China) and given chemotherapy without guidance of the ATP-TCA (control group). Before the third chemotherapeutic cycle, clinical outcomes were analyzed in the two groups. Results: Effective rate of hydrothorax in treatment group was 67%, while 46% in control group (P 〈 0.05). In refractory hydrothorax patients, they were 69% and 40% (P 〈 0.05), respectively.In vitro results correlated well with clinical outcomes (P 〈 0.01). Conclusion: Effective rate of chemotherapy for hydrothorax in NSCLC is higher in treatment group than that in control group. ATP-TCA is especially helpful for refractory hydrothorax. 展开更多
关键词 ATP based bioluminescence tumor chemosensitivity assay (ATP-TCA) non-small cell lung cancer (NSCLC)hydrothorax
下载PDF
Orotate phosphoribosyl transferase mRNA expression and the response of cholangiocarcinoma to 5-fluorouracil
2
作者 Chariya Hahnvajanawong Jariya Chaiyagool +6 位作者 Wunchana Seubwai Vajarabhongsa Bhudhisawasdi Nisana Namwat Narong Khuntikeo Banchob Sripa Ake Pugkhem Wichittra Tassaneeyakul 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第30期3955-3961,共7页
AIM:To determine whether expression of certain enzymes related to 5-fluorouracil(5-FU)metabolism predicts 5-FU chemosensitivity in cholangiocarcinoma(CCA).METHODS:The histoculture drug response assay(HDRA)was performe... AIM:To determine whether expression of certain enzymes related to 5-fluorouracil(5-FU)metabolism predicts 5-FU chemosensitivity in cholangiocarcinoma(CCA).METHODS:The histoculture drug response assay(HDRA)was performed using surgically resected CCA tissues.Tumor cell viability was determined morphologically with hematoxylin and eosin-and terminal deoxynucleotide transferase-mediated dUTP nick-end labeling-stained tissues.The mRNA expression of thymidine phosphorylase(TP),orotate phosphoribosyl transferase(OPRT),thymidylate synthase(TS),and dihydropyrimidine dehydrogenase(DPD)was determined with realtime reverse transcriptase-polymerase chain reaction.The levels of gene expression and the sensitivity to 5-FU were evaluated.RESULTS:Twenty-three CCA tissues were obtained from patients who had been diagnosed with intrahepatic CCA and who underwent surgical resection at Srinagarind Hospital,Khon Kaen University from 2007 to 2009.HDRA was used to determine the response of these CCA tissues to 5-FU.Based on the dose-response curve,200μg/mL 5-FU was selected as the test concentration.The percentage of inhibition index at the median point was selected as the cut-off point to differentiate the responding and non-responding tumors to 5-FU.When the relationship between TP,OPRT,TS and DPD mRNA expression levels and the sensitivity of CCA tissues to 5-FU was examined,only OPRT mRNA expression was significantly correlated with the response to 5-FU.The mean expression level of OPRT was significantly higher in the responder group compared to the non-responder group(0.41±0.25 vs 0.22±0.12,P<0.05).CONCLUSION:OPRT mRNA expression may be a useful predictor of 5-FU chemosensitivity of CCA.Whether OPRT mRNA could be used to predict the success of 5-FU chemotherapy in CCA patients requires confirmation in patients. 展开更多
关键词 Histoculture drug response assay 5-fluorouracil Cholangiocarcinoma Orotate phosphoribosyl transferase chemosensitivity
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部