AIM: To evaluate human pancreatic carcinoma cell line(PANC-1) cells apoptosis and Bcl-2 and Bax expression induced by Yin Chen Hao Decoction(YCHD).METHODS: The cell growth inhibitory rate was determined by MTT assay. ...AIM: To evaluate human pancreatic carcinoma cell line(PANC-1) cells apoptosis and Bcl-2 and Bax expression induced by Yin Chen Hao Decoction(YCHD).METHODS: The cell growth inhibitory rate was determined by MTT assay. Apoptosis of PANC-1 cells before and after treatment with YCHD was determined by TUNEL staining. Expression of the apoptosisassociated genes, Bcl-2 and Bax, was detected by immunohistochemical staining and reverse transcription-PCR.RESULTS: YCHD inhibited the growth of PANC-1 cells. Following treatment with YCHD for 24-96 h, the apoptotic rate of PANC-1 cells increased with time. In addition, the positive rate of Bcl-2 protein expression decreased in a time-dependent manner, whereas the positive rate of Bax protein expression increased in a time-dependent manner. Following treatment of with YCHD for 24-96 h, expression of BAX m RNA increased gradually and BCL-2 m RNA reduced gradually with time.CONCLUSION: YCHD induces apoptosis of PANC-1 cells mediated in part via up-regulation of BAX and down-regulation of BCL-2.展开更多
AIM To evaluate the therapeutic mechanism of Yinchenhao Chengqi (YCHCQ) decoction (containing mainly Herba Artemisia capillaris) combined with endoscopic sphincterotomy (EST) for endotoxemia (ETM) in acute cholangitis...AIM To evaluate the therapeutic mechanism of Yinchenhao Chengqi (YCHCQ) decoction (containing mainly Herba Artemisia capillaris) combined with endoscopic sphincterotomy (EST) for endotoxemia (ETM) in acute cholangitis.METHODS Twenty-one cases of acute cholangitis with ETM were divided randomly into two groups: group A, 10 patients treated with YCHCQ decoction combined with EST, group B, 11 patients treated with EST. The incidence rate of ETM, plasmic ET, serum superoxide dismutase (SOD) activity, malonyldialdehyde (MDA), complement C3 and C-reactive protein (CRP) were studied respectively.RESULTS The ET level of group A (35.92ng/L±8.30ng/L) was significantly reduced after 7 days of treatment (P＜0.05) in contrast to that of group B (47.8ng/L±11.62ng/L), so did the level of MDA and CRP. But the SOD activity and C3 level in group A increased significantly (P＜0.05).CONCLUSION YCHCQ decoction combined with EST had a beneficial effect for ETM in acute cholangitis.INTRODUCTIONEndotoxemia (ETM) is one of the most important physiopathologic causes of acute cholangitis and it is the trigger of cytokines and inflammatory factors. In recent studies it has been found that Yinchenhao Chengqi (YCHCQ) decoction has a beneficial effect on ETM in acute cholangitis. With the development of endoscopic surgery, endoscopic sphincterotomy (EST) has become an effective replacement for some operations in the treatment of acute cholangitis[1]. The effect of YCHCQ decoction combined with EST on ET, oxygen free radical and complement C3 was observed in order to find out its therapeutic mechanism.展开更多
目的:观察加味茵陈蒿汤对雌激素致肝内胆汁淤积症大鼠肝脏水通道蛋白8(AQP8)m RNA和蛋白表达的影响,探讨加味茵陈蒿汤治疗妊娠肝内胆汁淤积症可能的分子机制。方法:50只SD雌性大鼠,体质量180-200 g,随机分为正常组(N,n=10)和模型组(M’,...目的:观察加味茵陈蒿汤对雌激素致肝内胆汁淤积症大鼠肝脏水通道蛋白8(AQP8)m RNA和蛋白表达的影响,探讨加味茵陈蒿汤治疗妊娠肝内胆汁淤积症可能的分子机制。方法:50只SD雌性大鼠,体质量180-200 g,随机分为正常组(N,n=10)和模型组(M’,n=40)。M’组大鼠皮下注射17-α乙炔雌二醇5 mg·kg-1·d-1,连续5天,建立肝内胆汁淤积症模型,N组大鼠给予相同体积的丙二醇皮下注射。5天后,M’组大鼠随机分为模型组(M,n=10)、加味茵陈蒿汤高剂量(Zg,n=10)、中剂量(Zz,n=10)和低剂量组(Zd,n=10),分别给予生理盐水和高、中、低剂量的加味茵陈蒿汤灌胃,连续7天。7天后处死大鼠,留取肝脏组织,置于液氮中保存,分别运用实时定量PCR法及蛋白免疫印迹法检测AQP8 m RNA与蛋白表达情况。结果:与正常组(N)比较,模型组(M)大鼠肝脏组织AQP8 m RNA与蛋白表达水平降低(P<0.01)。与模型组(M)比较,高、中和低剂量的加味茵陈蒿汤均可增加大鼠肝脏组织AQP8 m RNA与蛋白表达水平(P<0.05)。此外,研究还发现,随着加味茵陈蒿汤给药剂量的增加,其上调AQP8 m RNA和蛋白表达的作用增强。结论:加味茵陈蒿汤治疗妊娠肝内胆汁淤积症的分子机制可能是通过增加肝脏组织AQP8 m RNA与蛋白表达水平实现的。展开更多
基金Supported by Traditional Chinese Medicine Administration of Zhejiang Province,China,No.2014ZA068
文摘AIM: To evaluate human pancreatic carcinoma cell line(PANC-1) cells apoptosis and Bcl-2 and Bax expression induced by Yin Chen Hao Decoction(YCHD).METHODS: The cell growth inhibitory rate was determined by MTT assay. Apoptosis of PANC-1 cells before and after treatment with YCHD was determined by TUNEL staining. Expression of the apoptosisassociated genes, Bcl-2 and Bax, was detected by immunohistochemical staining and reverse transcription-PCR.RESULTS: YCHD inhibited the growth of PANC-1 cells. Following treatment with YCHD for 24-96 h, the apoptotic rate of PANC-1 cells increased with time. In addition, the positive rate of Bcl-2 protein expression decreased in a time-dependent manner, whereas the positive rate of Bax protein expression increased in a time-dependent manner. Following treatment of with YCHD for 24-96 h, expression of BAX m RNA increased gradually and BCL-2 m RNA reduced gradually with time.CONCLUSION: YCHD induces apoptosis of PANC-1 cells mediated in part via up-regulation of BAX and down-regulation of BCL-2.
文摘AIM To evaluate the therapeutic mechanism of Yinchenhao Chengqi (YCHCQ) decoction (containing mainly Herba Artemisia capillaris) combined with endoscopic sphincterotomy (EST) for endotoxemia (ETM) in acute cholangitis.METHODS Twenty-one cases of acute cholangitis with ETM were divided randomly into two groups: group A, 10 patients treated with YCHCQ decoction combined with EST, group B, 11 patients treated with EST. The incidence rate of ETM, plasmic ET, serum superoxide dismutase (SOD) activity, malonyldialdehyde (MDA), complement C3 and C-reactive protein (CRP) were studied respectively.RESULTS The ET level of group A (35.92ng/L±8.30ng/L) was significantly reduced after 7 days of treatment (P＜0.05) in contrast to that of group B (47.8ng/L±11.62ng/L), so did the level of MDA and CRP. But the SOD activity and C3 level in group A increased significantly (P＜0.05).CONCLUSION YCHCQ decoction combined with EST had a beneficial effect for ETM in acute cholangitis.INTRODUCTIONEndotoxemia (ETM) is one of the most important physiopathologic causes of acute cholangitis and it is the trigger of cytokines and inflammatory factors. In recent studies it has been found that Yinchenhao Chengqi (YCHCQ) decoction has a beneficial effect on ETM in acute cholangitis. With the development of endoscopic surgery, endoscopic sphincterotomy (EST) has become an effective replacement for some operations in the treatment of acute cholangitis[1]. The effect of YCHCQ decoction combined with EST on ET, oxygen free radical and complement C3 was observed in order to find out its therapeutic mechanism.
文摘目的:观察加味茵陈蒿汤对雌激素致肝内胆汁淤积症大鼠肝脏水通道蛋白8(AQP8)m RNA和蛋白表达的影响,探讨加味茵陈蒿汤治疗妊娠肝内胆汁淤积症可能的分子机制。方法:50只SD雌性大鼠,体质量180-200 g,随机分为正常组(N,n=10)和模型组(M’,n=40)。M’组大鼠皮下注射17-α乙炔雌二醇5 mg·kg-1·d-1,连续5天,建立肝内胆汁淤积症模型,N组大鼠给予相同体积的丙二醇皮下注射。5天后,M’组大鼠随机分为模型组(M,n=10)、加味茵陈蒿汤高剂量(Zg,n=10)、中剂量(Zz,n=10)和低剂量组(Zd,n=10),分别给予生理盐水和高、中、低剂量的加味茵陈蒿汤灌胃,连续7天。7天后处死大鼠,留取肝脏组织,置于液氮中保存,分别运用实时定量PCR法及蛋白免疫印迹法检测AQP8 m RNA与蛋白表达情况。结果:与正常组(N)比较,模型组(M)大鼠肝脏组织AQP8 m RNA与蛋白表达水平降低(P<0.01)。与模型组(M)比较,高、中和低剂量的加味茵陈蒿汤均可增加大鼠肝脏组织AQP8 m RNA与蛋白表达水平(P<0.05)。此外,研究还发现,随着加味茵陈蒿汤给药剂量的增加,其上调AQP8 m RNA和蛋白表达的作用增强。结论:加味茵陈蒿汤治疗妊娠肝内胆汁淤积症的分子机制可能是通过增加肝脏组织AQP8 m RNA与蛋白表达水平实现的。
