Low-pressure discharge is applied for stimulation of germination of two seed lots of Lamb＇s Quarters （Chenopodium album agg.） with different starting germinations （17%, 8%） and in different stages of dormancy. Di...Low-pressure discharge is applied for stimulation of germination of two seed lots of Lamb＇s Quarters （Chenopodium album agg.） with different starting germinations （17%, 8%） and in different stages of dormancy. Different exposition durations with cold plasma treatment were applied. The variable of the ratio cumulative germination was calculated. The Richards＇ equation was used for curve-fitting and simulation of the growth curves. Population parameters, namely Vi - viability, Me - time, Qu - dispersion, and Sk - skewness, counted from the curves described the germination rate well. Significant differences among Qu confirmed the erratic dormancy and gradual germination of Lamb＇s Quarters. No difference in the Me parameter was found between two tested seed lots, and no interspecies characteristics were changed using low-pressure discharge. The results suggested that plasma treatment changed seed germination in Lamb＇s Quarters seeds.展开更多
[Objectives]This study was conducted to identify the microscopic characteristics of Chenopodium album L.[Methods]The microscopic identification method was adopted.[Results]The xylem vessels and fiber bundles of the ro...[Objectives]This study was conducted to identify the microscopic characteristics of Chenopodium album L.[Methods]The microscopic identification method was adopted.[Results]The xylem vessels and fiber bundles of the roots are arranged into 3-4 intermittent ring belts in a concave-convex pattern,alternately with the parenchymal cell ring belts;and the xylem rays vary in width.The cross section of the stems is polygonal;there are parenchyma cells at the corners of the cortex;there are many vascular bundles of varying sizes;and calcium oxalate clusters are common in the medulla and cortex.There are 2 to 4 vascular bundles in the main leaf vein,peltate;and fiber bundles exist below the phloem.The powder is characterized by numerous clusters of calcium oxalate,which are uniform in size,sharp at edges and corners.[Conclusions]This study can provide reference for the identification and quality standard of the crude drug.展开更多
The genus Chenopodium comprises about 150 species, of which Chenopodium quinoa and C. album are important for their nutritional value. Evaluation of variation in qualitative morphological traits of plants and SNPs in ...The genus Chenopodium comprises about 150 species, of which Chenopodium quinoa and C. album are important for their nutritional value. Evaluation of variation in qualitative morphological traits of plants and SNPs in chloroplast rbc L and mat K gene sequences in 19 accessions representing C. quinoa and C. album indicated that the accessions IC-411824 and IC-411825,which have white seeds, belong to C. quinoa rather than C. album. This observation was also supported by a time tree that indicated IC-411824 and IC-411825 to be a sister clade to accessions of C. quinoa with an estimated age of 1.2 Mya. Whereas multiple alignments of rbc L gene sequences from the 19 accessions revealed 1.26% parsimony-informative sites with 0.68%interspecific sequence diversity, alignment of nucleotide sequences of amplicons representing the mat K gene revealed 4.97% parsimony-informative sites and 2.81% interspecific sequence diversity. Validation of SNPs in the cp rbc L and mat K regions of 36 accessions belonging to C. quinoa and C. album was performed by allele-specific PCR with primers carrying a single base change at the 3′ end. We report the first C. quinoa-specific SNP-based primer, R1RQ-AFR,designed from rbc L sequences, that could differentiate quinoa from 64 genera including13 species of the genus Chenopodium. With an estimated age of 10.5–4.1 million years(Myr), the Himalayan chenopods are evolutionarily younger than the Andean chenopods. The results establish the paraphyletic origin of the genus Chenopodium.展开更多
应用正、反相硅胶和葡聚糖凝胶柱色谱等技术对藜(Chenopodium album L.)的黄酮类化学成分进行了分离和纯化.从藜中分离得到2种黄酮类化合物,分别为异鼠李素3-O-芸香糖苷(1)、槲皮素3-O-β-D-呋喃芹糖(1→2)-O-[α-L-鼠李糖(1→6)]-β-D...应用正、反相硅胶和葡聚糖凝胶柱色谱等技术对藜(Chenopodium album L.)的黄酮类化学成分进行了分离和纯化.从藜中分离得到2种黄酮类化合物,分别为异鼠李素3-O-芸香糖苷(1)、槲皮素3-O-β-D-呋喃芹糖(1→2)-O-[α-L-鼠李糖(1→6)]-β-D-葡萄糖苷(2).采用纸片扩散法进行了抑菌试验.结果表明,黎的95%(体积分数)乙醇提取液对大肠埃希菌有明显的抑菌效果,最低抑菌浓度(MIC)为32mg/L.展开更多
基金supported by the Institutional Research Plan No.AV0Z60870520(erá)the research plans MSM 0021620834(Tichy)MSM 4674788501(patenka) that are financed by the Ministry of Education,Youth and Sports of the Czech Republic
文摘Low-pressure discharge is applied for stimulation of germination of two seed lots of Lamb＇s Quarters （Chenopodium album agg.） with different starting germinations （17%, 8%） and in different stages of dormancy. Different exposition durations with cold plasma treatment were applied. The variable of the ratio cumulative germination was calculated. The Richards＇ equation was used for curve-fitting and simulation of the growth curves. Population parameters, namely Vi - viability, Me - time, Qu - dispersion, and Sk - skewness, counted from the curves described the germination rate well. Significant differences among Qu confirmed the erratic dormancy and gradual germination of Lamb＇s Quarters. No difference in the Me parameter was found between two tested seed lots, and no interspecies characteristics were changed using low-pressure discharge. The results suggested that plasma treatment changed seed germination in Lamb＇s Quarters seeds.
基金Supported by General Project of Guangxi Science and Technology Department(2020GXNSFAA259043)Yao Medicine Quality Standard Project(MZY2017001)+1 种基金First-class Discipline in Guangxi of Traditional Chinese Pharmacology(GJKY[2018]12)Fund of Guangxi University of Chinese Medicine(05018028F2)。
文摘[Objectives]This study was conducted to identify the microscopic characteristics of Chenopodium album L.[Methods]The microscopic identification method was adopted.[Results]The xylem vessels and fiber bundles of the roots are arranged into 3-4 intermittent ring belts in a concave-convex pattern,alternately with the parenchymal cell ring belts;and the xylem rays vary in width.The cross section of the stems is polygonal;there are parenchyma cells at the corners of the cortex;there are many vascular bundles of varying sizes;and calcium oxalate clusters are common in the medulla and cortex.There are 2 to 4 vascular bundles in the main leaf vein,peltate;and fiber bundles exist below the phloem.The powder is characterized by numerous clusters of calcium oxalate,which are uniform in size,sharp at edges and corners.[Conclusions]This study can provide reference for the identification and quality standard of the crude drug.
基金Financial support received from Department of Biotechnology,Government of India vide grant No.BT/PR-8953/BCE/08/533/2007project sanctioned against grant No.BT/04/NE/2009financial support from Department of Science&Technology,Government of India in the form of a research fellowship under the INSPIRE program
文摘The genus Chenopodium comprises about 150 species, of which Chenopodium quinoa and C. album are important for their nutritional value. Evaluation of variation in qualitative morphological traits of plants and SNPs in chloroplast rbc L and mat K gene sequences in 19 accessions representing C. quinoa and C. album indicated that the accessions IC-411824 and IC-411825,which have white seeds, belong to C. quinoa rather than C. album. This observation was also supported by a time tree that indicated IC-411824 and IC-411825 to be a sister clade to accessions of C. quinoa with an estimated age of 1.2 Mya. Whereas multiple alignments of rbc L gene sequences from the 19 accessions revealed 1.26% parsimony-informative sites with 0.68%interspecific sequence diversity, alignment of nucleotide sequences of amplicons representing the mat K gene revealed 4.97% parsimony-informative sites and 2.81% interspecific sequence diversity. Validation of SNPs in the cp rbc L and mat K regions of 36 accessions belonging to C. quinoa and C. album was performed by allele-specific PCR with primers carrying a single base change at the 3′ end. We report the first C. quinoa-specific SNP-based primer, R1RQ-AFR,designed from rbc L sequences, that could differentiate quinoa from 64 genera including13 species of the genus Chenopodium. With an estimated age of 10.5–4.1 million years(Myr), the Himalayan chenopods are evolutionarily younger than the Andean chenopods. The results establish the paraphyletic origin of the genus Chenopodium.
文摘应用正、反相硅胶和葡聚糖凝胶柱色谱等技术对藜(Chenopodium album L.)的黄酮类化学成分进行了分离和纯化.从藜中分离得到2种黄酮类化合物,分别为异鼠李素3-O-芸香糖苷(1)、槲皮素3-O-β-D-呋喃芹糖(1→2)-O-[α-L-鼠李糖(1→6)]-β-D-葡萄糖苷(2).采用纸片扩散法进行了抑菌试验.结果表明,黎的95%(体积分数)乙醇提取液对大肠埃希菌有明显的抑菌效果,最低抑菌浓度(MIC)为32mg/L.
