To rapidly obtain high-quality genomic DNA from Chenopodium quinoa Willd, the genomic DAN in different tissues （leaves, stems and roots） of Chenopodi- um quinoa Willd was extracted by modified CTAB method, SDS metho...To rapidly obtain high-quality genomic DNA from Chenopodium quinoa Willd, the genomic DAN in different tissues （leaves, stems and roots） of Chenopodi- um quinoa Willd was extracted by modified CTAB method, SDS method and high- salt Iow-pH method, respectively. The quality and yield of extracted DNA was deter- mined using agarose gel electrophoresis and UV spectrophotometry. At the same time, the PCR-SSR and SSCP molecular detection was also performed. The results showed that the gel test strips, without obvious decomposition, of all the extraction methods were relatively obvious; the genomic DNA yield extracted by modified CTAB method was highest, followed by that by SDS method, and the genomic DNA extracted by high-salt Iow-pH method was lowest： the genomic DNA yields extracted by different methods from Chenopodium quinoa Wiltd leaves were all high- er than those from roots and stems; the quality of Chenopodium quinoa Willd ge- nomic DNA extracted by modified CTAB method and high-salt Iow-pH method was better, and polyphenols, polysaccharides and other impurities were removed more completely. The PCR-SSR and SSCP detection results showed that the genomic DNA extracted by different methods from different tissues of Chenopodium quinoa Willd all could be better amplified, and high-quality strips could be obtained. So the Chenopodium quinoa Willd genomic DNA extracted by the three methods all can be used for subsequent molecular biology research.展开更多
Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was opti...Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was optimized, as well as the callus proliferation system. Research results showed that the optimal explant for callus induction was stem segment. The average callus induction rate of nine varieties reached 90% in culture medium MS ＋ 0.5 mg/L 2, 4-D. In the callus opti- mization test, treatment VI （MS ＋ 0.5 mg/L 2, 4-D ＋ 0.5 mg/L KT ＋ 0.5 mg/L NAA） and treatment II （MS ＋ 0.5 mg/L 2, 4-D） had close induction rate, but the callus morphology was greatly different. The latter had loose, glossy and yellowish white calluses. Therefore, culture medium MS ＋ 0.5 mg/L 2, 4-D was the optimal for callus induction. And using 2, 4-D together with KT and NAA could significantly increase the proliferation rate of calluses.展开更多
In this paper,based on the study of Chenopodium quinoa Willd.planting,it is concluded that the current situations of large-scale development of C.quinoa Willd.include the following four aspects:first,the research on C...In this paper,based on the study of Chenopodium quinoa Willd.planting,it is concluded that the current situations of large-scale development of C.quinoa Willd.include the following four aspects:first,the research on C.quinoa Willd.varieties needs to be strengthened;second,it is insufficient to master the training environment and cultivation techniques;third,the large-scale planting of C.quinoa Willd.is not enough;fourth,the degree of mechanization of C.quinoa Willd.planting is not enough.In view of the above situation,this paper puts forward the following effective suggestions to strengthen the large-scale development of C.quinoa Willd.:the first is to increase the investment in C.quinoa Willd.variety research;the second is to strengthen the analysis of introduction and screening of C.quinoa Willd.varieties;the third is to study the best planting environment and cultivation techniques of C.quinoa Willd.;the fourth is to increase the scale of mechanized production of C.quinoa Willd.;the fifth is to increase the research and development of C.quinoa Willd.related products and their deep processing technology.展开更多
This study was conducted to find a method for rapid determination of fat content in complete quinoa （ Chenopodium quinoa Willd） seeds. The near infrared spectra of 100 quinoa samples were collected, and a mathematic...This study was conducted to find a method for rapid determination of fat content in complete quinoa （ Chenopodium quinoa Willd） seeds. The near infrared spectra of 100 quinoa samples were collected, and a mathematic model was built using the near infrared spectra, so as to perform prediction. The results showed that within the wavelength range of 1 0 000-4 000 cm ^-1 , the quantification model of fat content built by first derivative ＋vector normalization spectral pre-processing had better calibration and prediction effects, and showed a determination coefficient of cross validation （ r cv^ 2 ） of 0.939 3 and a determination coefficient of validation （ rval^2 ） of 0.923 5. The near infrared spectral model of fat could be used for rapid detection of fat contents in quinoa.展开更多
This study was conducted to establish a method for rapid determination of crude starch content in complete quinoa （ Chenopodium quinoa Willd） seeds. The near infrared spectra of 100 quinoa samples were collected, an...This study was conducted to establish a method for rapid determination of crude starch content in complete quinoa （ Chenopodium quinoa Willd） seeds. The near infrared spectra of 100 quinoa samples were collected, and a mathematic model was built using the near infrared spectra within the wavelength range of 1 0 000-4 000 cm^-1 by first derivative ＋vector normalization spectral pre-processing. The results showed that the quantification model of starch content had better calibration and prediction effects, and showed a determination coefficient of cross validation （ r^2 cv ） of 0.914 7 and a determination coefficient of validation （ r^2 val ） of 0.903 1. The determination of starch content in complete quinoa seeds by near infrared spectroscopy is totally feasible.展开更多
[Objective] To explore a rapid determination method for fiber content in grains of quinoa. [Method] Near infrared spectra of 100 quinoa samples were collected. The predicted models for quantitative analysis of fiber c...[Objective] To explore a rapid determination method for fiber content in grains of quinoa. [Method] Near infrared spectra of 100 quinoa samples were collected. The predicted models for quantitative analysis of fiber contents in the grains were built using near infrared transmittance spectroscopy （NITS）. [Result] In the wavelength range of 10 000-4 000 cm-1, the near infrared quantitative model of quinoa crude fiber was set up via first derivative ＋ vector normalization preprocessing and combining with the data from chemical methods. The calibration and prediction effect were best, and then the cross validation determination coefficient （FFcv） and external validation determination coefficient （FFval） of fiber by near in- frared quantitative model were 0.884 8 and 0.876 1, respectively. [Conclusion] the model of NITS about complete grains quinoa fiber can be available for fast detecting quinoa fiber content.展开更多
为研究硼(B)对藜麦生理特性的影响,进行喷施硼肥盆栽试验。选用3个藜麦品种(晋藜1号、晋藜2号、晋藜3号),在拔节期进行叶面喷施0%(CK)、0.1%(B_(1))、0.2%(B_(2))、0.3%(B_(3))的硼酸溶液50 mL 2次。结果表明,相同生长期内,随着施硼量...为研究硼(B)对藜麦生理特性的影响,进行喷施硼肥盆栽试验。选用3个藜麦品种(晋藜1号、晋藜2号、晋藜3号),在拔节期进行叶面喷施0%(CK)、0.1%(B_(1))、0.2%(B_(2))、0.3%(B_(3))的硼酸溶液50 mL 2次。结果表明,相同生长期内,随着施硼量的增加,藜麦叶片SOD活性逐渐下降,POD活性先升高后降低,CAT活性逐渐升高。相同浓度条件下,随生长期延长,3种酶活性均表现为先升高后降低。在灌浆期,3个藜麦品种SOD活性最高;硼浓度为0.3%时,与对照相比,3个藜麦品种叶片SOD活性分别降低21.24%、15.26%、10.33%。整体来看,3个藜麦品种叶片POD活性在抽穗期最高;硼浓度为0.3%时,与对照相比,3个藜麦品种叶片POD活性分别提高-3.99%、-5.21%、5.28%。3个藜麦品种叶片CAT活性在灌浆期最高;硼浓度为0.3%时,与对照相比,3个藜麦品种叶片CAT活性分别提高25.31%、26.19%、29.48%。说明施硼可以调节藜麦抗氧化酶水平,提高藜麦的抗逆性,缓解缺硼对藜麦生长造成的不利影响。总体来看,当硼浓度为0.3%时,3个藜麦品种生理特性良好。实际生产中,应选用0.3%的硼较合适。展开更多
基金Supported by National Natural Science Foundation of China(31301372)Key Project of Science and Technology Plan of Zhejiang Province(2011C12030)Innovation Training Project of Zhejiang Agriculture and Forestry University(201301004)~~
文摘To rapidly obtain high-quality genomic DNA from Chenopodium quinoa Willd, the genomic DAN in different tissues （leaves, stems and roots） of Chenopodi- um quinoa Willd was extracted by modified CTAB method, SDS method and high- salt Iow-pH method, respectively. The quality and yield of extracted DNA was deter- mined using agarose gel electrophoresis and UV spectrophotometry. At the same time, the PCR-SSR and SSCP molecular detection was also performed. The results showed that the gel test strips, without obvious decomposition, of all the extraction methods were relatively obvious; the genomic DNA yield extracted by modified CTAB method was highest, followed by that by SDS method, and the genomic DNA extracted by high-salt Iow-pH method was lowest： the genomic DNA yields extracted by different methods from Chenopodium quinoa Wiltd leaves were all high- er than those from roots and stems; the quality of Chenopodium quinoa Willd ge- nomic DNA extracted by modified CTAB method and high-salt Iow-pH method was better, and polyphenols, polysaccharides and other impurities were removed more completely. The PCR-SSR and SSCP detection results showed that the genomic DNA extracted by different methods from different tissues of Chenopodium quinoa Willd all could be better amplified, and high-quality strips could be obtained. So the Chenopodium quinoa Willd genomic DNA extracted by the three methods all can be used for subsequent molecular biology research.
基金Supported by National Natural Sciences Foundation of China(31301372)the Major Science and Technology Project of Zhejiang Province(2011C12030)the Haixi Prefecture science and technology project of Qinghai Province(2012-Y01)~~
文摘Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was optimized, as well as the callus proliferation system. Research results showed that the optimal explant for callus induction was stem segment. The average callus induction rate of nine varieties reached 90% in culture medium MS ＋ 0.5 mg/L 2, 4-D. In the callus opti- mization test, treatment VI （MS ＋ 0.5 mg/L 2, 4-D ＋ 0.5 mg/L KT ＋ 0.5 mg/L NAA） and treatment II （MS ＋ 0.5 mg/L 2, 4-D） had close induction rate, but the callus morphology was greatly different. The latter had loose, glossy and yellowish white calluses. Therefore, culture medium MS ＋ 0.5 mg/L 2, 4-D was the optimal for callus induction. And using 2, 4-D together with KT and NAA could significantly increase the proliferation rate of calluses.
文摘In this paper,based on the study of Chenopodium quinoa Willd.planting,it is concluded that the current situations of large-scale development of C.quinoa Willd.include the following four aspects:first,the research on C.quinoa Willd.varieties needs to be strengthened;second,it is insufficient to master the training environment and cultivation techniques;third,the large-scale planting of C.quinoa Willd.is not enough;fourth,the degree of mechanization of C.quinoa Willd.planting is not enough.In view of the above situation,this paper puts forward the following effective suggestions to strengthen the large-scale development of C.quinoa Willd.:the first is to increase the investment in C.quinoa Willd.variety research;the second is to strengthen the analysis of introduction and screening of C.quinoa Willd.varieties;the third is to study the best planting environment and cultivation techniques of C.quinoa Willd.;the fourth is to increase the scale of mechanized production of C.quinoa Willd.;the fifth is to increase the research and development of C.quinoa Willd.related products and their deep processing technology.
基金Supported by Special Fund for the Protection and Utilization of Crop Germplasm Resources of the Ministry of Agriculture(2017NWB036-20)Key Project of Shanxi Academy of Agricultural Sciences(YGG17064)Key Research Plan Project of Shanxi Province(201603D21102)
文摘This study was conducted to find a method for rapid determination of fat content in complete quinoa （ Chenopodium quinoa Willd） seeds. The near infrared spectra of 100 quinoa samples were collected, and a mathematic model was built using the near infrared spectra, so as to perform prediction. The results showed that within the wavelength range of 1 0 000-4 000 cm ^-1 , the quantification model of fat content built by first derivative ＋vector normalization spectral pre-processing had better calibration and prediction effects, and showed a determination coefficient of cross validation （ r cv^ 2 ） of 0.939 3 and a determination coefficient of validation （ rval^2 ） of 0.923 5. The near infrared spectral model of fat could be used for rapid detection of fat contents in quinoa.
基金Supported by Special Fund for the Protection and Utilization of Crop Germplasm Resources of the Ministry of Agriculture(2017NWB036-20)Key Project of Shanxi Academy of Agricultural Sciences(YGG17064)Key Research Plan Project of Shanxi Province(201603D21102)
文摘This study was conducted to establish a method for rapid determination of crude starch content in complete quinoa （ Chenopodium quinoa Willd） seeds. The near infrared spectra of 100 quinoa samples were collected, and a mathematic model was built using the near infrared spectra within the wavelength range of 1 0 000-4 000 cm^-1 by first derivative ＋vector normalization spectral pre-processing. The results showed that the quantification model of starch content had better calibration and prediction effects, and showed a determination coefficient of cross validation （ r^2 cv ） of 0.914 7 and a determination coefficient of validation （ r^2 val ） of 0.903 1. The determination of starch content in complete quinoa seeds by near infrared spectroscopy is totally feasible.
基金Supported by the Collection and Arrangement of Crop Germplasm Resources in Shanxi Province(2016zzcx-17)the Special Fund for the Protection and Utilization of Crop Germplasm Resources of the Ministry of Agriculture(2015NWB030-07)+1 种基金the Project of the National Science and Technology Infrastructure of the Ministry of Science and Technology and the Ministry of Finance(NICGR2015-026)the Special Fund for Seed Industry of Shanxi Province(2016zyzx41)~~
文摘[Objective] To explore a rapid determination method for fiber content in grains of quinoa. [Method] Near infrared spectra of 100 quinoa samples were collected. The predicted models for quantitative analysis of fiber contents in the grains were built using near infrared transmittance spectroscopy （NITS）. [Result] In the wavelength range of 10 000-4 000 cm-1, the near infrared quantitative model of quinoa crude fiber was set up via first derivative ＋ vector normalization preprocessing and combining with the data from chemical methods. The calibration and prediction effect were best, and then the cross validation determination coefficient （FFcv） and external validation determination coefficient （FFval） of fiber by near in- frared quantitative model were 0.884 8 and 0.876 1, respectively. [Conclusion] the model of NITS about complete grains quinoa fiber can be available for fast detecting quinoa fiber content.
文摘为研究硼(B)对藜麦生理特性的影响,进行喷施硼肥盆栽试验。选用3个藜麦品种(晋藜1号、晋藜2号、晋藜3号),在拔节期进行叶面喷施0%(CK)、0.1%(B_(1))、0.2%(B_(2))、0.3%(B_(3))的硼酸溶液50 mL 2次。结果表明,相同生长期内,随着施硼量的增加,藜麦叶片SOD活性逐渐下降,POD活性先升高后降低,CAT活性逐渐升高。相同浓度条件下,随生长期延长,3种酶活性均表现为先升高后降低。在灌浆期,3个藜麦品种SOD活性最高;硼浓度为0.3%时,与对照相比,3个藜麦品种叶片SOD活性分别降低21.24%、15.26%、10.33%。整体来看,3个藜麦品种叶片POD活性在抽穗期最高;硼浓度为0.3%时,与对照相比,3个藜麦品种叶片POD活性分别提高-3.99%、-5.21%、5.28%。3个藜麦品种叶片CAT活性在灌浆期最高;硼浓度为0.3%时,与对照相比,3个藜麦品种叶片CAT活性分别提高25.31%、26.19%、29.48%。说明施硼可以调节藜麦抗氧化酶水平,提高藜麦的抗逆性,缓解缺硼对藜麦生长造成的不利影响。总体来看,当硼浓度为0.3%时,3个藜麦品种生理特性良好。实际生产中,应选用0.3%的硼较合适。
