Manganese enhances the expression of the manganese superoxide dismutase in cultured primary chick embryonic myocardial cells

In the present study, the effect of manganese（Mn） on antioxidant status and the expression of the manganese superoxide dismutase（MnSOD） gene in cultured primary myocardial cells collected from the chick embryos was investigated. The hypothesis that Mn supplementation would enhance the expression of MnSOD in cultured primary myocardial cells of chick embryos was tested. Eggs collected from Mn-depleted Arbor Acres laying breeder hens were incubated for 10 days and then myocardial cells were isolated and cultivated for 8 days. The embryonic myocardial cells on day 6 were treated with Mn in the cell culture medium at different time points when the proportion of cells showing spontaneous contraction was over 95% after the 3-day primary culture. A completely randomized design involving a 3 Mn levels（0, 0.5 and 1.0 mmol L^（-1））×3 incubation time points（12, 24 and 48 h） factorial arrangement of treatments（n=6） was used in the current experiment. The results showed that MnSOD activity and m RNA expression level were induced by Mn and increased with incubation time, which supported the hypothesis that Mn would enhance the expression of the MnSOD gene, and thus might protect myocardial cells from oxidative stress during the chick embryonic development.

Forsythiaside A Induced IFN-α and Up-regulated the Correlated Factors in JAK-STAT Signaling Pathway in vitro

[Objective] This study was to investigate the effect of forsythiaside A added in chick embryo kidney cells (CEKs), on the changes in expression of interferon α(IFN-α) and mRNA expression level of correlated factors in JAK-STAT pathway. [Methods] Three levels of forsythiaside (100, 200, 400 μg/ml) were adopted to treat the experimental materials, then the expression levels of IFN-α and correlated factors in JAK-STAT pathway were detected by real-time RT-PCR at the transcriptional level (mRNA) and by Western blot at the translation level(IFN-α protein). [Result] Compared with the control group (untreated group), Application of forsythiaside A not only significantly increased the expression of IFN-α in treated CEKs (P<0.05) , but also up-regulated the expression of STAT1, JAK1, IFNAR1, IFNAR2, IRF1 and IRF7, the correlated factors in JAK-STAT pathway. [Conclusion] Forsythiaside A induced the expression of IFN-α in CEKs, and can positively regulate the JAK-STAT signaling pathway significantly.