Chicken is one of the most popular meat products in the world. Salmonella Typhimurium is a common foodbome pathogens associated with the processing of poultry. An optical Surface Plasmon Resonance (SPR) biosensor wa...Chicken is one of the most popular meat products in the world. Salmonella Typhimurium is a common foodbome pathogens associated with the processing of poultry. An optical Surface Plasmon Resonance (SPR) biosensor was sensitive to the presence of Salmonella Typhimurium in chicken carcass. The Spreeta biosensor kits were used to detect Salmonella Typhimurium on chicken carcass successfully. A taste sensor like electronic tongue or biosensors was used to basically "taste" the object and differentiated one object from the other with different taste sensor signatures. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with dif- ferent antisera or other molecular species. The selectivity of the SPR biosensor was assayed using a series of antibody con- centrations and dilution series of the organism. The SPR biosensor showed promising to detect the existence of Salmonella Typhimurium at 1 x 106 CFU/ml. Initial results show that the SPR biosensor has the potential for its application in pathogenic bacteria monitoring. However, more tests need to be done to confirm the detection limitation.展开更多
The study evaluated the growth performance and carcass quality of COBB500 broilers fed two experimental rations incorporating feed and mango fine ED<sub>10</sub> for a batch of 83 birds, ED<sub>20<...The study evaluated the growth performance and carcass quality of COBB500 broilers fed two experimental rations incorporating feed and mango fine ED<sub>10</sub> for a batch of 83 birds, ED<sub>20</sub> (batch of 83 birds) in comparison with a commercial control ration CCD (84 birds). For this purpose, 250 day-old chicks of this breed were purchased in Korhogo and transported to Tengréla in Ivory Coast for rearing. The results show that substituting mango provender ans and mango seed flour for maize resulted in rations with costs per kgMS of 215.35FCFA, 2016.93FCFA and 320FCFA for the ED<sub>10</sub>, ED<sub>20</sub> and CCD rations respectively. The production costs per kg of chicken (FCPkgLW) of the CCD ration were significantly higher than those of the ED10 and ED<sub>20</sub> (p 0.05). ADGs were significantly different and decreasing from CCD, ED<sub>20</sub> to ED<sub>10</sub> (p 0.05). Average weights at 42nd day for ED<sub>20</sub> and CCD were homogeneous (p > 0.05) and significantly higher than for ED<sub>10</sub> (p 0.05). By day 49th the average weight of ED<sub>20</sub> had reached and exceeded that of CCD by day 42nd. PSLW, HCW and CCW were significantly different and decreasing from CCW, ED<sub>20</sub> to ED<sub>10</sub> (p 0.05). Average hot (HCY) and cooled (CCY) carcass yields of ED<sub>20</sub> and CCD rations were comparable (p > 0.05) and significantly higher than those of ED<sub>10</sub> ration (p = 0.009) and 0.003). Average drumstick and neck weights were significantly higher and lower for CCD, ED<sub>20</sub> and ED<sub>10</sub> (p 0.05). For all other parts of the cut, the average weights of the CCD ration were significantly higher (p 0.05) than those of the ED<sub>20</sub> and ED<sub>10</sub> rations, which were homogeneous (p 0.05). The incorporation of mango provender and mango seed flour in broiler rations offers the prospect of reduced production costs, less competition between man and animals over cereals, and lean meat production that is more appreciated than that of chickens fed conventional feed. As it stands, the ED<sub>20</sub> ration can be recommended as a growth diet for broilers. It would be necessary to determine the levels of anti-nutritional substances in mango feeds and to assess the in vivo digestibility of mango ingredients and the diets incorporating them.展开更多
The selection of meat quality has received considerable focus in chicken breeding. This study was aimed at investigating the effect of CAPN1 gene on meat quality traits in chicken populations. Primer pairs for 3 UTR i...The selection of meat quality has received considerable focus in chicken breeding. This study was aimed at investigating the effect of CAPN1 gene on meat quality traits in chicken populations. Primer pairs for 3 UTR in CAPN1 were designed from database of chicken genomic sequence. Polymorphisms were detected using PCR-SSCP and DNA sequencing. A mutation at position 9 950 nt (G/A, locus A) was found among individuals in each population. The allele and genotype frequencies significantly differed among eight lines with higher frequencies of allele A2 and genotype AIA2 (P〈0.01). The least square analysis showed that there was significant difference (P 〈 0.05) in muscle fiber density and some carcass traits among genotypes and that the breast muscle fiber density (BFD) of birds ofAIA1 genotype was significantly higher (P〈0.05) than that of birds ofA2A2 genotype. It was concluded that the CAPN1 gene was the major gene affecting the muscle fiber traits of chicken or was linked with the major gene. These results were useful for studying the molecular mechanism that influences meat traits and were used as the base of molecular-assisted selection to meat quality traits. So, this site may be a potential marker affecting the muscle traits of chickens.展开更多
为了探究脂肪量和肥胖相关(fat mass and obesity associated,FTO)基因多态性及其对鸡屠体性状和生长性状的影响,试验采用DNA测序检测鸡FTO基因编码区的单核苷酸多态性(SNP),并采用一般线性模型中的LSD法分析FTO基因多态性对和盈黑鸡屠...为了探究脂肪量和肥胖相关(fat mass and obesity associated,FTO)基因多态性及其对鸡屠体性状和生长性状的影响,试验采用DNA测序检测鸡FTO基因编码区的单核苷酸多态性(SNP),并采用一般线性模型中的LSD法分析FTO基因多态性对和盈黑鸡屠体性状和生长性状的影响。结果表明:在FTO基因第5外显子和第7外显子上各检测到一个SNP位点,分别为g.57337C>A和g.64757T>G突变。g.57337C>A突变形成AA、AB和BB 3种基因型,g.64757T>G突变形成TT、TG和GG 3种基因型。g.57337C>A位点对和盈黑鸡屠体重、宰前活重、头重、胸肌重、腿肌重上、肝脏重、心脏重7个屠体性状有显著或极显著影响(P<0.05或P<0.01),对8,10,16周龄体重影响显著(P<0.05)。g.64757T>G位点对和盈黑鸡宰前活重、全净膛重、半净膛重、屠体重和翅重5个屠体性状有显著影响(P<0.05),对16周龄体重影响显著(P<0.05)。说明FTO基因g.57337C>A和g.64757T>G位点可作为和盈黑鸡屠体性状和生长性状遗传改良的重要候选分子标记。展开更多
Growth performance, carcass characteristics and meat quality of male chickens were evaluated using eight-week-old 360 cockerels from three exotic (Koekoek, Kuroiler and Sasso-R) and one Ethiopian indigenous (Horro) ge...Growth performance, carcass characteristics and meat quality of male chickens were evaluated using eight-week-old 360 cockerels from three exotic (Koekoek, Kuroiler and Sasso-R) and one Ethiopian indigenous (Horro) genotypes. Cockerels in each breed (90) were randomly allocated into three replications of 30 birds each and housed in deep litter pens for the experiment that lasted for 8 to</span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">16 weeks. The experiment was arranged in a Completely Randomized Design. Seven birds per breed were slaughtered at the end to determine carcass yield, chemical composition and quality attributes of meat. </span><span style="font-family:Verdana;">The Kuroiler breed has higher body weight</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> body weight change (</span><span style="font-family:Verdana;">1621.8 </span><span style="font-family:Verdana;">g)</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> average daily gain (</span><span style="font-family:Verdana;">28.9 g/day),</span><span style="font-family:Verdana;"> daily feed consumption (</span><span style="font-family:Verdana;">127.4 g/day) and better feed conversion ratio (4.40) than other breed</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;">. The Kuroiler breed has heavier </span><span style="font-family:Verdana;">slaughter weight (</span><span style="font-family:Verdana;">2716.3 </span><span style="font-family:Verdana;">g)</span><span style="font-family:Verdana;">,</span><span> </span><span style="font-family:Verdana;">dressed carcass weight (2201.0 g), eviscerated carcass weight (1905.1 g), breast weight (537.7 g), thigh weight (303.1 g) and drumstick weight (274.8 g) than other breeds. Total edible offal was higher </span><span style="font-family:Verdana;">for </span><span style="font-family:Verdana;">Kuroiler (324.2 g) and Sasso-R (306.1 g), intermediate in Koekoek (204.4 g) and low in Horro (152.4 g) breed. The relative proportion of breast and drumstick (% slaughter weight) were lower in Koekoek and Sasso-R (17.3 and 8.9) breeds, respectively. Abdominal fat weight (13.7</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">g and 1.02%) was lowest in Horro. </span><span style="font-family:Verdana;">Sasso-R showed higher percent edible offal weight (</span><span style="font-family:Verdana;">13.4%) than</span><span style="font-family:Verdana;"> Horro breed (</span><span style="font-family:Verdana;">11.4%). The DM content in breast and thigh meat was higher for Horro breed than the rest. The CP content in the breast and thigh meat was lower for the Kuroiler and Koekoek breeds, respectively. Higher EE in the thigh meat was recorded for Sasso-R (8.9%), whereas, EE content in the breast meat was similar (3.4%</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">-</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">3.7%) among the breeds. F</span><span style="font-family:Verdana;">lavor</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> tenderness</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> juiciness and general acceptability of breast and thigh meat were not affected by breed. </span><span style="font-family:Verdana;">The highest net return was observed in the Kuroiler breed. </span><span style="font-family:Verdana;">Based on the results obtained</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> it is suggested that </span><span style="font-family:Verdana;">the Kuroiler breed can be used for cockerel meat production in Ethiopia where intensive broiler production is not well developed.展开更多
文摘Chicken is one of the most popular meat products in the world. Salmonella Typhimurium is a common foodbome pathogens associated with the processing of poultry. An optical Surface Plasmon Resonance (SPR) biosensor was sensitive to the presence of Salmonella Typhimurium in chicken carcass. The Spreeta biosensor kits were used to detect Salmonella Typhimurium on chicken carcass successfully. A taste sensor like electronic tongue or biosensors was used to basically "taste" the object and differentiated one object from the other with different taste sensor signatures. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with dif- ferent antisera or other molecular species. The selectivity of the SPR biosensor was assayed using a series of antibody con- centrations and dilution series of the organism. The SPR biosensor showed promising to detect the existence of Salmonella Typhimurium at 1 x 106 CFU/ml. Initial results show that the SPR biosensor has the potential for its application in pathogenic bacteria monitoring. However, more tests need to be done to confirm the detection limitation.
文摘The study evaluated the growth performance and carcass quality of COBB500 broilers fed two experimental rations incorporating feed and mango fine ED<sub>10</sub> for a batch of 83 birds, ED<sub>20</sub> (batch of 83 birds) in comparison with a commercial control ration CCD (84 birds). For this purpose, 250 day-old chicks of this breed were purchased in Korhogo and transported to Tengréla in Ivory Coast for rearing. The results show that substituting mango provender ans and mango seed flour for maize resulted in rations with costs per kgMS of 215.35FCFA, 2016.93FCFA and 320FCFA for the ED<sub>10</sub>, ED<sub>20</sub> and CCD rations respectively. The production costs per kg of chicken (FCPkgLW) of the CCD ration were significantly higher than those of the ED10 and ED<sub>20</sub> (p 0.05). ADGs were significantly different and decreasing from CCD, ED<sub>20</sub> to ED<sub>10</sub> (p 0.05). Average weights at 42nd day for ED<sub>20</sub> and CCD were homogeneous (p > 0.05) and significantly higher than for ED<sub>10</sub> (p 0.05). By day 49th the average weight of ED<sub>20</sub> had reached and exceeded that of CCD by day 42nd. PSLW, HCW and CCW were significantly different and decreasing from CCW, ED<sub>20</sub> to ED<sub>10</sub> (p 0.05). Average hot (HCY) and cooled (CCY) carcass yields of ED<sub>20</sub> and CCD rations were comparable (p > 0.05) and significantly higher than those of ED<sub>10</sub> ration (p = 0.009) and 0.003). Average drumstick and neck weights were significantly higher and lower for CCD, ED<sub>20</sub> and ED<sub>10</sub> (p 0.05). For all other parts of the cut, the average weights of the CCD ration were significantly higher (p 0.05) than those of the ED<sub>20</sub> and ED<sub>10</sub> rations, which were homogeneous (p 0.05). The incorporation of mango provender and mango seed flour in broiler rations offers the prospect of reduced production costs, less competition between man and animals over cereals, and lean meat production that is more appreciated than that of chickens fed conventional feed. As it stands, the ED<sub>20</sub> ration can be recommended as a growth diet for broilers. It would be necessary to determine the levels of anti-nutritional substances in mango feeds and to assess the in vivo digestibility of mango ingredients and the diets incorporating them.
文摘The selection of meat quality has received considerable focus in chicken breeding. This study was aimed at investigating the effect of CAPN1 gene on meat quality traits in chicken populations. Primer pairs for 3 UTR in CAPN1 were designed from database of chicken genomic sequence. Polymorphisms were detected using PCR-SSCP and DNA sequencing. A mutation at position 9 950 nt (G/A, locus A) was found among individuals in each population. The allele and genotype frequencies significantly differed among eight lines with higher frequencies of allele A2 and genotype AIA2 (P〈0.01). The least square analysis showed that there was significant difference (P 〈 0.05) in muscle fiber density and some carcass traits among genotypes and that the breast muscle fiber density (BFD) of birds ofAIA1 genotype was significantly higher (P〈0.05) than that of birds ofA2A2 genotype. It was concluded that the CAPN1 gene was the major gene affecting the muscle fiber traits of chicken or was linked with the major gene. These results were useful for studying the molecular mechanism that influences meat traits and were used as the base of molecular-assisted selection to meat quality traits. So, this site may be a potential marker affecting the muscle traits of chickens.
文摘为了探究脂肪量和肥胖相关(fat mass and obesity associated,FTO)基因多态性及其对鸡屠体性状和生长性状的影响,试验采用DNA测序检测鸡FTO基因编码区的单核苷酸多态性(SNP),并采用一般线性模型中的LSD法分析FTO基因多态性对和盈黑鸡屠体性状和生长性状的影响。结果表明:在FTO基因第5外显子和第7外显子上各检测到一个SNP位点,分别为g.57337C>A和g.64757T>G突变。g.57337C>A突变形成AA、AB和BB 3种基因型,g.64757T>G突变形成TT、TG和GG 3种基因型。g.57337C>A位点对和盈黑鸡屠体重、宰前活重、头重、胸肌重、腿肌重上、肝脏重、心脏重7个屠体性状有显著或极显著影响(P<0.05或P<0.01),对8,10,16周龄体重影响显著(P<0.05)。g.64757T>G位点对和盈黑鸡宰前活重、全净膛重、半净膛重、屠体重和翅重5个屠体性状有显著影响(P<0.05),对16周龄体重影响显著(P<0.05)。说明FTO基因g.57337C>A和g.64757T>G位点可作为和盈黑鸡屠体性状和生长性状遗传改良的重要候选分子标记。
文摘Growth performance, carcass characteristics and meat quality of male chickens were evaluated using eight-week-old 360 cockerels from three exotic (Koekoek, Kuroiler and Sasso-R) and one Ethiopian indigenous (Horro) genotypes. Cockerels in each breed (90) were randomly allocated into three replications of 30 birds each and housed in deep litter pens for the experiment that lasted for 8 to</span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">16 weeks. The experiment was arranged in a Completely Randomized Design. Seven birds per breed were slaughtered at the end to determine carcass yield, chemical composition and quality attributes of meat. </span><span style="font-family:Verdana;">The Kuroiler breed has higher body weight</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> body weight change (</span><span style="font-family:Verdana;">1621.8 </span><span style="font-family:Verdana;">g)</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> average daily gain (</span><span style="font-family:Verdana;">28.9 g/day),</span><span style="font-family:Verdana;"> daily feed consumption (</span><span style="font-family:Verdana;">127.4 g/day) and better feed conversion ratio (4.40) than other breed</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;">. The Kuroiler breed has heavier </span><span style="font-family:Verdana;">slaughter weight (</span><span style="font-family:Verdana;">2716.3 </span><span style="font-family:Verdana;">g)</span><span style="font-family:Verdana;">,</span><span> </span><span style="font-family:Verdana;">dressed carcass weight (2201.0 g), eviscerated carcass weight (1905.1 g), breast weight (537.7 g), thigh weight (303.1 g) and drumstick weight (274.8 g) than other breeds. Total edible offal was higher </span><span style="font-family:Verdana;">for </span><span style="font-family:Verdana;">Kuroiler (324.2 g) and Sasso-R (306.1 g), intermediate in Koekoek (204.4 g) and low in Horro (152.4 g) breed. The relative proportion of breast and drumstick (% slaughter weight) were lower in Koekoek and Sasso-R (17.3 and 8.9) breeds, respectively. Abdominal fat weight (13.7</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">g and 1.02%) was lowest in Horro. </span><span style="font-family:Verdana;">Sasso-R showed higher percent edible offal weight (</span><span style="font-family:Verdana;">13.4%) than</span><span style="font-family:Verdana;"> Horro breed (</span><span style="font-family:Verdana;">11.4%). The DM content in breast and thigh meat was higher for Horro breed than the rest. The CP content in the breast and thigh meat was lower for the Kuroiler and Koekoek breeds, respectively. Higher EE in the thigh meat was recorded for Sasso-R (8.9%), whereas, EE content in the breast meat was similar (3.4%</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">-</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">3.7%) among the breeds. F</span><span style="font-family:Verdana;">lavor</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> tenderness</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> juiciness and general acceptability of breast and thigh meat were not affected by breed. </span><span style="font-family:Verdana;">The highest net return was observed in the Kuroiler breed. </span><span style="font-family:Verdana;">Based on the results obtained</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> it is suggested that </span><span style="font-family:Verdana;">the Kuroiler breed can be used for cockerel meat production in Ethiopia where intensive broiler production is not well developed.