Objective:To observe the effectiveness and safety of one-time endodontics in the treatment of chronic apical periodontitis with sinus tract in pediatric deciduous teeth.Methods:109 cases of children with chronic apica...Objective:To observe the effectiveness and safety of one-time endodontics in the treatment of chronic apical periodontitis with sinus tract in pediatric deciduous teeth.Methods:109 cases of children with chronic apical periodontitis with sinus tract in the deciduous teeth treated in our hospital from January 2022 to December 2023 were selected and grouped by the randomized numerical table method,with 54 cases in the experimental group and 55 cases in the control group.The experimental group was treated with one-time endodontics and the control group was treated with conventional endodontics.Results:After the treatment,the total effective rate of treatment was higher in the experimental group than in the control group(P<0.05);the incidence of adverse events was lower in the experimental group than in the control group(P<0.05);the satisfaction of the children's family members was higher in the experimental group than in the control group(P<0.05);the pain duration was lower in the experimental group than in the control group(P<0.05).Conclusion:In the experimental group,children with chronic apical periodontitis with sinus tract of the deciduous teeth were given one-time endodontic treatment,and the results of its implementation were relatively good.展开更多
AIM: To investigate the effect of stem cells from human exfoliated deciduous teeth(SHED) transplanted for bone regeneration in the dog mandibular defect.METHODS: In this prospective comparative study, SHEDs had been i...AIM: To investigate the effect of stem cells from human exfoliated deciduous teeth(SHED) transplanted for bone regeneration in the dog mandibular defect.METHODS: In this prospective comparative study, SHEDs had been isolated 5 years ago from human exfoliated deciduous teeth. The undifferentiated stem cells were seeded into mandibular bone through-andthrough defects of 4 dogs. Similar defects in control group were filled with cell-free collagen scaffold. After 12 wk, biopsies were taken and morphometric analysis was performed. The percentage of new bone formation and foreign body reaction were measured in each case. The data were subject to statistical analysis using the Mann-Whitney U and Kruskalwalis statistical tests. Differences at P < 0.05 was considered as significant level.RESULTS: There were no significant differences between control and SHED-seeded groups in connective tissue(P = 0.248), woven bone(P = 0.248) and compact bone(P = 0.082). There were not any side effects in transplanted SHED group such as teratoma or malignancy and abnormalities in this period.CONCLUSION: SHEDs which had been isolated and characterized 5 years ago and stored with cryopreservation banking were capable of proliferation and osteogenesis after 5 years, and no immune response was observed after three months of seeded SHEDs.展开更多
BACKGROUND Autoimmune hepatitis is a serious autoimmune liver disease that threatens human health worldwide,which emphasizes the urgent need to identify novel treatments.Stem cells from human exfoliated deciduous teet...BACKGROUND Autoimmune hepatitis is a serious autoimmune liver disease that threatens human health worldwide,which emphasizes the urgent need to identify novel treatments.Stem cells from human exfoliated deciduous teeth(SHED),which are easy to obtain in a non-invasive manner,show pronounced proliferative and immunomodulatory capacities.AIM To investigate the protective effects of SHED on concanavalin A(ConA)-induced hepatitis in mice,and to elucidate the associated regulatory mechanisms.METHODS We used a ConA-induced acute hepatitis mouse model and an in vitro co-culture system to study the protective effects of SHED on ConA-induced autoimmune hepatitis,as well as the associated underlying mechanisms.RESULTS SHED infusion could prevent aberrant histopathological liver architecture caused by ConA-induced infiltration of CD3+,CD4+,tumor necrosis-alpha+,and interferon-gamma+inflammatory cells.Alanine aminotransferase and aspartate aminotransferase were significantly elevated in hepatitis mice.SHED infusion could therefore block ConA-induced alanine aminotransferase and aspartate aminotransferase elevations.Mechanistically,ConA upregulated tumor necrosisalpha and interferon-gamma expression,which was activated by the nuclear factor-kappa B pathway to induce hepatocyte apoptosis,resulting in acute liver injury.SHED administration protected hepatocytes from ConA-induced apoptosis.CONCLUSION SHED alleviates ConA-induced acute liver injury via inhibition of hepatocyte apoptosis mediated by the nuclear factor-kappa B pathway.Our findings could provide a potential treatment strategy for hepatitis.展开更多
目的比较Vitapex糊剂与氢氧化钙糊剂根管填充治疗乳牙牙髓炎患儿的效果差异。方法选取2022年8月至2023年6月电子科技大学医学院附属妇女儿童医院·成都市妇女儿童中心医院口腔门诊诊治的130例(130颗牙)乳牙牙髓炎患儿作为研究对象,...目的比较Vitapex糊剂与氢氧化钙糊剂根管填充治疗乳牙牙髓炎患儿的效果差异。方法选取2022年8月至2023年6月电子科技大学医学院附属妇女儿童医院·成都市妇女儿童中心医院口腔门诊诊治的130例(130颗牙)乳牙牙髓炎患儿作为研究对象,根据就诊单双号将其分为试验组和对照组(每组65例),试验组患儿根管填充治疗采用Vitapex糊剂、对照组患儿根管填充治疗采用氢氧化钙糊剂,对比两组患儿治疗前后疼痛程度、龈沟液中炎症因子水平、牙龈情况及咀嚼效率、咀嚼功能、临床效果的差异。结果治疗后3、7、14 d,两组患儿的疼痛明显缓解,试验组患儿治疗后3、7 d的视觉模拟疼痛量表(VAS)评分均低于对照组(3.32±0.89 vs 3.94±0.94,1.64±0.60 vs 1.90±0.57,P<0.05);治疗后7 d两组患儿炎症程度均明显缓解,患儿的IL-1β、IL-8、TNF-α、IL-6水平均明显降低,试验组患儿低于对照组(14.30±2.77 vs 18.32±4.13,25.41±4.80 vs 28.32±5.02,2.84±0.77 vs 3.36±0.95,13.04±2.20 vs 15.46±2.89,P<0.05);治疗7 d后,两组患儿咬合时间明显缩短、咬合力明显提高,试验组患儿的咬合力提高更明显(133.2±14.8 vs 121.8±15.0,P<0.05);治疗2个月后,试验组痊愈率为64.62%、显效率为33.85%、有效率为1.54%,对照组痊愈率为44.62%、显效率为49.23%、有效率为6.15%,试验组疗效明显优于对照组(P<0.05)。结论Vitapex糊剂与氢氧化钙糊剂根管填充治疗乳牙牙髓炎患儿,均能缓解炎症反应程度及疼痛程度,但是前者具有更好的消炎止痛效果,总体疗效更好。展开更多
目的探讨氟化钠护齿剂对幼儿园儿童乳牙患龋率和龋面变化的影响。方法选取2017年1月本地区10所公办幼儿园共330名儿童作为研究对象,随机分为三组,每组各110名儿童,A组给予氟化钠护齿剂(含5%氟化钠)0.2 m L/次,1次/年;B组给予入氟化钠护...目的探讨氟化钠护齿剂对幼儿园儿童乳牙患龋率和龋面变化的影响。方法选取2017年1月本地区10所公办幼儿园共330名儿童作为研究对象,随机分为三组,每组各110名儿童,A组给予氟化钠护齿剂(含5%氟化钠)0.2 m L/次,1次/年;B组给予入氟化钠护齿剂0.1 m L/次,1次/年;C组儿童作为空白对照组,记录涂氟后三组儿童2年内乳牙患龋率、龋面均、新增龋面变化、致龋菌检出情况以及涂氟后尿氟浓度变化。结果A组与B组干预后第2年龋齿发生率分别为37.27%、39.09%低于C组51.82%(P<0.05);A组、B组第1年、第2年龋均值低于C组(P<0.05);A组与B组儿童涂氟后第1年与第2年乳牙颊舌面、邻面、牙合面新生龋面均值低于C组(P<0.05);A组与B组儿童干预第2年变形链球菌、远缘链球菌、乳杆菌检出率低于C组(P<0.05);A组、B组儿童涂氟后6 h、12 h尿氟浓度高于C组儿童(P<0.05);三组儿童涂氟后24 h、48 h尿氟浓度比较差异无统计学意义(P>0.05);A组与B组儿童涂氟后龋齿发生率、龋均值、新生龋面、致龋菌检出率比较差异无统计学意义(P>0.05)。结论氟化钠的应用可降低幼儿园儿童龋齿发生率,减少新生龋面,降低致龋菌检出率,无需涂布过多剂量的氟化钠即可达到较好的防龋效果,安全性较高。展开更多
文摘Objective:To observe the effectiveness and safety of one-time endodontics in the treatment of chronic apical periodontitis with sinus tract in pediatric deciduous teeth.Methods:109 cases of children with chronic apical periodontitis with sinus tract in the deciduous teeth treated in our hospital from January 2022 to December 2023 were selected and grouped by the randomized numerical table method,with 54 cases in the experimental group and 55 cases in the control group.The experimental group was treated with one-time endodontics and the control group was treated with conventional endodontics.Results:After the treatment,the total effective rate of treatment was higher in the experimental group than in the control group(P<0.05);the incidence of adverse events was lower in the experimental group than in the control group(P<0.05);the satisfaction of the children's family members was higher in the experimental group than in the control group(P<0.05);the pain duration was lower in the experimental group than in the control group(P<0.05).Conclusion:In the experimental group,children with chronic apical periodontitis with sinus tract of the deciduous teeth were given one-time endodontic treatment,and the results of its implementation were relatively good.
文摘AIM: To investigate the effect of stem cells from human exfoliated deciduous teeth(SHED) transplanted for bone regeneration in the dog mandibular defect.METHODS: In this prospective comparative study, SHEDs had been isolated 5 years ago from human exfoliated deciduous teeth. The undifferentiated stem cells were seeded into mandibular bone through-andthrough defects of 4 dogs. Similar defects in control group were filled with cell-free collagen scaffold. After 12 wk, biopsies were taken and morphometric analysis was performed. The percentage of new bone formation and foreign body reaction were measured in each case. The data were subject to statistical analysis using the Mann-Whitney U and Kruskalwalis statistical tests. Differences at P < 0.05 was considered as significant level.RESULTS: There were no significant differences between control and SHED-seeded groups in connective tissue(P = 0.248), woven bone(P = 0.248) and compact bone(P = 0.082). There were not any side effects in transplanted SHED group such as teratoma or malignancy and abnormalities in this period.CONCLUSION: SHEDs which had been isolated and characterized 5 years ago and stored with cryopreservation banking were capable of proliferation and osteogenesis after 5 years, and no immune response was observed after three months of seeded SHEDs.
基金Supported by The National Natural Science Foundation of China,No.81970940 and No.81600865Beijing Natural Science Foundation,No.7182182and the National Science and Technology Major Project of the Ministry of Science and Technology of China,No.2018ZX10302207.
文摘BACKGROUND Autoimmune hepatitis is a serious autoimmune liver disease that threatens human health worldwide,which emphasizes the urgent need to identify novel treatments.Stem cells from human exfoliated deciduous teeth(SHED),which are easy to obtain in a non-invasive manner,show pronounced proliferative and immunomodulatory capacities.AIM To investigate the protective effects of SHED on concanavalin A(ConA)-induced hepatitis in mice,and to elucidate the associated regulatory mechanisms.METHODS We used a ConA-induced acute hepatitis mouse model and an in vitro co-culture system to study the protective effects of SHED on ConA-induced autoimmune hepatitis,as well as the associated underlying mechanisms.RESULTS SHED infusion could prevent aberrant histopathological liver architecture caused by ConA-induced infiltration of CD3+,CD4+,tumor necrosis-alpha+,and interferon-gamma+inflammatory cells.Alanine aminotransferase and aspartate aminotransferase were significantly elevated in hepatitis mice.SHED infusion could therefore block ConA-induced alanine aminotransferase and aspartate aminotransferase elevations.Mechanistically,ConA upregulated tumor necrosisalpha and interferon-gamma expression,which was activated by the nuclear factor-kappa B pathway to induce hepatocyte apoptosis,resulting in acute liver injury.SHED administration protected hepatocytes from ConA-induced apoptosis.CONCLUSION SHED alleviates ConA-induced acute liver injury via inhibition of hepatocyte apoptosis mediated by the nuclear factor-kappa B pathway.Our findings could provide a potential treatment strategy for hepatitis.
文摘目的比较Vitapex糊剂与氢氧化钙糊剂根管填充治疗乳牙牙髓炎患儿的效果差异。方法选取2022年8月至2023年6月电子科技大学医学院附属妇女儿童医院·成都市妇女儿童中心医院口腔门诊诊治的130例(130颗牙)乳牙牙髓炎患儿作为研究对象,根据就诊单双号将其分为试验组和对照组(每组65例),试验组患儿根管填充治疗采用Vitapex糊剂、对照组患儿根管填充治疗采用氢氧化钙糊剂,对比两组患儿治疗前后疼痛程度、龈沟液中炎症因子水平、牙龈情况及咀嚼效率、咀嚼功能、临床效果的差异。结果治疗后3、7、14 d,两组患儿的疼痛明显缓解,试验组患儿治疗后3、7 d的视觉模拟疼痛量表(VAS)评分均低于对照组(3.32±0.89 vs 3.94±0.94,1.64±0.60 vs 1.90±0.57,P<0.05);治疗后7 d两组患儿炎症程度均明显缓解,患儿的IL-1β、IL-8、TNF-α、IL-6水平均明显降低,试验组患儿低于对照组(14.30±2.77 vs 18.32±4.13,25.41±4.80 vs 28.32±5.02,2.84±0.77 vs 3.36±0.95,13.04±2.20 vs 15.46±2.89,P<0.05);治疗7 d后,两组患儿咬合时间明显缩短、咬合力明显提高,试验组患儿的咬合力提高更明显(133.2±14.8 vs 121.8±15.0,P<0.05);治疗2个月后,试验组痊愈率为64.62%、显效率为33.85%、有效率为1.54%,对照组痊愈率为44.62%、显效率为49.23%、有效率为6.15%,试验组疗效明显优于对照组(P<0.05)。结论Vitapex糊剂与氢氧化钙糊剂根管填充治疗乳牙牙髓炎患儿,均能缓解炎症反应程度及疼痛程度,但是前者具有更好的消炎止痛效果,总体疗效更好。
文摘目的探讨氟化钠护齿剂对幼儿园儿童乳牙患龋率和龋面变化的影响。方法选取2017年1月本地区10所公办幼儿园共330名儿童作为研究对象,随机分为三组,每组各110名儿童,A组给予氟化钠护齿剂(含5%氟化钠)0.2 m L/次,1次/年;B组给予入氟化钠护齿剂0.1 m L/次,1次/年;C组儿童作为空白对照组,记录涂氟后三组儿童2年内乳牙患龋率、龋面均、新增龋面变化、致龋菌检出情况以及涂氟后尿氟浓度变化。结果A组与B组干预后第2年龋齿发生率分别为37.27%、39.09%低于C组51.82%(P<0.05);A组、B组第1年、第2年龋均值低于C组(P<0.05);A组与B组儿童涂氟后第1年与第2年乳牙颊舌面、邻面、牙合面新生龋面均值低于C组(P<0.05);A组与B组儿童干预第2年变形链球菌、远缘链球菌、乳杆菌检出率低于C组(P<0.05);A组、B组儿童涂氟后6 h、12 h尿氟浓度高于C组儿童(P<0.05);三组儿童涂氟后24 h、48 h尿氟浓度比较差异无统计学意义(P>0.05);A组与B组儿童涂氟后龋齿发生率、龋均值、新生龋面、致龋菌检出率比较差异无统计学意义(P>0.05)。结论氟化钠的应用可降低幼儿园儿童龋齿发生率,减少新生龋面,降低致龋菌检出率,无需涂布过多剂量的氟化钠即可达到较好的防龋效果,安全性较高。
