Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands...Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands and 12 specific ISSR bands were scored,and a cluster dendrogram was constructed.The 90 wild strains of Agaricus were separated into two major groups,the first consisting of wild strains of A.bisporus and the second consisting of other Agaricus strains.Forty-one wild strains of A.bisporus collected in Sichuan and Tibet formed four groups according to the geographical location of the collection area.White A.bisporus strains from Xinjiang and Tibet(Xinjiang Wild,AgX04 and AgX042)exhibited unique band patterns resulting in lower similarity coefficient values(10%～13%)when compared with brown or light-brown A.bisporus strains.展开更多
依据苞片是覆瓦状排列或是卷筒状排列而把姜花属Hedychium分为两个亚属的分类法近年颇受质疑。本文利用30对多态性好的随机引物,对中国姜花属的19种1变种共22份材料进行SRAP分子标记分析。其中最有效的28对引物共扩出152条带,当中135条...依据苞片是覆瓦状排列或是卷筒状排列而把姜花属Hedychium分为两个亚属的分类法近年颇受质疑。本文利用30对多态性好的随机引物,对中国姜花属的19种1变种共22份材料进行SRAP分子标记分析。其中最有效的28对引物共扩出152条带,当中135条为多态性带,占88.8%。平均每对4.8条,多态性条带里最多的为引物组合F13R1,达到13条。聚类分析表明:(1)中国姜花属植物可分为三个类群:第I群植株较矮小,主要分布于石灰岩地区;第II群与第I群每苞片均具2朵以上的小花,但植株较高大且基本上不分布于石灰岩地区;第III群每苞片仅具1朵小花。此结果与Wood(2000)用ITS分析得出的结果基本一致。(2)支持吴德邻和Larsen(2000)把H.emeienseZ.Y.Zhu归并为峨眉姜花H.flavescens Carey ex Roscoe的观点。(3)盘珠姜花H.panzhuumZ.Y.Zhu与黄姜花H.flavum Roxb.是同一种植物,即盘珠姜花是黄姜花的晚出同名。(4)土壤基质可能是导致姜花属物种分化的重要因素。作者认为根据每苞片有花多少的特征,在系统学上可把姜花属分为两个类群:A类群,每苞片仅有1朵小花;B类群,每苞片有2朵以上的小花。展开更多
基金Supported by National Fund of Natural Sciences(No.30671456,30771503)Key Science & Technology Programsof Fujian Province(No.2006S1001)+1 种基金Fujian Fund of Natural Sciences(No.B0620004)Key S&T InnovationItems of Fujian Academy of Agricultural Sciences(2007)
文摘Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands and 12 specific ISSR bands were scored,and a cluster dendrogram was constructed.The 90 wild strains of Agaricus were separated into two major groups,the first consisting of wild strains of A.bisporus and the second consisting of other Agaricus strains.Forty-one wild strains of A.bisporus collected in Sichuan and Tibet formed four groups according to the geographical location of the collection area.White A.bisporus strains from Xinjiang and Tibet(Xinjiang Wild,AgX04 and AgX042)exhibited unique band patterns resulting in lower similarity coefficient values(10%～13%)when compared with brown or light-brown A.bisporus strains.
文摘依据苞片是覆瓦状排列或是卷筒状排列而把姜花属Hedychium分为两个亚属的分类法近年颇受质疑。本文利用30对多态性好的随机引物,对中国姜花属的19种1变种共22份材料进行SRAP分子标记分析。其中最有效的28对引物共扩出152条带,当中135条为多态性带,占88.8%。平均每对4.8条,多态性条带里最多的为引物组合F13R1,达到13条。聚类分析表明:(1)中国姜花属植物可分为三个类群:第I群植株较矮小,主要分布于石灰岩地区;第II群与第I群每苞片均具2朵以上的小花,但植株较高大且基本上不分布于石灰岩地区;第III群每苞片仅具1朵小花。此结果与Wood(2000)用ITS分析得出的结果基本一致。(2)支持吴德邻和Larsen(2000)把H.emeienseZ.Y.Zhu归并为峨眉姜花H.flavescens Carey ex Roscoe的观点。(3)盘珠姜花H.panzhuumZ.Y.Zhu与黄姜花H.flavum Roxb.是同一种植物,即盘珠姜花是黄姜花的晚出同名。(4)土壤基质可能是导致姜花属物种分化的重要因素。作者认为根据每苞片有花多少的特征,在系统学上可把姜花属分为两个类群:A类群,每苞片仅有1朵小花;B类群,每苞片有2朵以上的小花。
