As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits ...As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD)(P=0.04) and live weight (LW)(P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW)(P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P<0.05) than that of the fine wool (F) group. Moreover, it had a higher expression level (P<0.01) in skin tissues than in other tissues of Chinese Merino ewes. While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos. Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS).展开更多
Zircon dating,geochemical and Nd-Sr isotopic analyses have been determined for samples from two granitic intrusions in the Talate mining district,Chinese Altay.Our data suggest that these intrusions were emplaced from...Zircon dating,geochemical and Nd-Sr isotopic analyses have been determined for samples from two granitic intrusions in the Talate mining district,Chinese Altay.Our data suggest that these intrusions were emplaced from 462.5 Ma to 457.8 Ma.These rocks have strong affinity to peralumious S-type granite and are characterized by prominent negative Eu anomalies(δEu=0.20–0.35),strong depletion in Ba,Sr,P,Ti,Nb,Ta and positive anomalies in Rb,Th,U,K,La,Nd,Zr,Hf.Nd-Sr isotopic compositions of the whole rock show negativeεNd(t)values(-1.21 to-0.08)and Mesoproterozoic Nd model ages(T2 DM=1.20–1.30 Ga).Their precursor magmas were likely derived from the partial dehydration melting of Mesoproterozoic mica-rich pelitic sources and mixed with minor mantle-derived components,under relatively low P(≤1 kbar)and high T(746–796℃)conditions.A ridge subduction model may account for the early Paleozoic geodynamic process with mantle-derived magmas caused by Ordovician ridge subduction and the opening of a slab window underplated and/or intraplated in the middle–upper crust,which triggered extensive partial melting of the shallow crust to generate diverse igneous rocks,and provided the heat for the crustal melting and juvenile materials for crustal growth.展开更多
基金supported by the National Natural Science Foundation of China (31360543 and 31760655)the earmarked fund for the China Agriculture Research System (CARS-39)+1 种基金the Open Project of Key Laboratory of Genetics Breeding and Reproduction of Xinjiang Cashmere and Wool Sheep, China (2016D03017)the Postdoctoral Science Foundation, China (2017M623287)
文摘As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD)(P=0.04) and live weight (LW)(P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW)(P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P<0.05) than that of the fine wool (F) group. Moreover, it had a higher expression level (P<0.01) in skin tissues than in other tissues of Chinese Merino ewes. While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos. Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS).
基金financially supported the National Key S&T Support Program of China (2006BAB07B02-01, 02)the Fundamental Research Funds for Scientific Institution (JYYWF20180104)Geological investigation (DD20190398)
文摘Zircon dating,geochemical and Nd-Sr isotopic analyses have been determined for samples from two granitic intrusions in the Talate mining district,Chinese Altay.Our data suggest that these intrusions were emplaced from 462.5 Ma to 457.8 Ma.These rocks have strong affinity to peralumious S-type granite and are characterized by prominent negative Eu anomalies(δEu=0.20–0.35),strong depletion in Ba,Sr,P,Ti,Nb,Ta and positive anomalies in Rb,Th,U,K,La,Nd,Zr,Hf.Nd-Sr isotopic compositions of the whole rock show negativeεNd(t)values(-1.21 to-0.08)and Mesoproterozoic Nd model ages(T2 DM=1.20–1.30 Ga).Their precursor magmas were likely derived from the partial dehydration melting of Mesoproterozoic mica-rich pelitic sources and mixed with minor mantle-derived components,under relatively low P(≤1 kbar)and high T(746–796℃)conditions.A ridge subduction model may account for the early Paleozoic geodynamic process with mantle-derived magmas caused by Ordovician ridge subduction and the opening of a slab window underplated and/or intraplated in the middle–upper crust,which triggered extensive partial melting of the shallow crust to generate diverse igneous rocks,and provided the heat for the crustal melting and juvenile materials for crustal growth.
