[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific reference...[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific references for the further study on the functions of KAP6.1 gene and molecular breeding of fine wool sheep. [Method] By using real-time fluorescence quantitative PCR, the expression map of KAP6.1 gene in various tissues was analyzed, and then PCR-SSCP technology, cloning and sequencing were adopted to analyze the polymorphism of KAP6.1 gene in 693 Chinese merino sheep （Xinjiang Junken type）, finally the correlation between KAPS. 1 gene and wool traits was discussed. [ Result] A high -level expression of KAP6.1 gene appeared in sheep skin, very significantly higher than that in muscle, small intestines, ovaries, hearts, lungs, livers, spleens, kidneys and rumen （ P 〈0.01 ）. In addition, there was C159T base substitution in KAF6.1 gene sequence, and analysis of the least squares model showed that the mean wool fleece weight of BB genotype at C159T site was significantly higher than that of AA and AB genotype （ P 〈0.05）, while there was no significant difference in average wool fiber diameter, curvature, length, clean fleece rate and density among hA, BB and AB genotype of KAP6.1 gene at C159T site （P〉0.05）. [Conclusion] KAP6.1 gene could be as the candidate gene of wool yield of sheep, and BB genotype could be used as the important molecular marker of fine wool sheep for high wool yield.展开更多
基金supported by Genetically Modified Organisms Breeding Major Projects ( 2009ZX08009-160B) Science and Technology Guidance Plan of Xinjiang Academy of Agricultural and Reclamation Sciences (YYD2010-8)+1 种基金 Science and Technology Key Project of Bintuan Agriculture (2009GG17,2011BA006) Doctoral Foundation of Bintuan (2011BB015)
文摘[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific references for the further study on the functions of KAP6.1 gene and molecular breeding of fine wool sheep. [Method] By using real-time fluorescence quantitative PCR, the expression map of KAP6.1 gene in various tissues was analyzed, and then PCR-SSCP technology, cloning and sequencing were adopted to analyze the polymorphism of KAP6.1 gene in 693 Chinese merino sheep （Xinjiang Junken type）, finally the correlation between KAPS. 1 gene and wool traits was discussed. [ Result] A high -level expression of KAP6.1 gene appeared in sheep skin, very significantly higher than that in muscle, small intestines, ovaries, hearts, lungs, livers, spleens, kidneys and rumen （ P 〈0.01 ）. In addition, there was C159T base substitution in KAF6.1 gene sequence, and analysis of the least squares model showed that the mean wool fleece weight of BB genotype at C159T site was significantly higher than that of AA and AB genotype （ P 〈0.05）, while there was no significant difference in average wool fiber diameter, curvature, length, clean fleece rate and density among hA, BB and AB genotype of KAP6.1 gene at C159T site （P〉0.05）. [Conclusion] KAP6.1 gene could be as the candidate gene of wool yield of sheep, and BB genotype could be used as the important molecular marker of fine wool sheep for high wool yield.
