As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits ...As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD)(P=0.04) and live weight (LW)(P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW)(P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P<0.05) than that of the fine wool (F) group. Moreover, it had a higher expression level (P<0.01) in skin tissues than in other tissues of Chinese Merino ewes. While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos. Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS).展开更多
The aim of our present study was to construct genetic structure and relationships among Chinese fine-wool sheep breeds. 46 individuals from 25 breeds or strains were genotyped based on the Illumina Ovine 50K SNP array...The aim of our present study was to construct genetic structure and relationships among Chinese fine-wool sheep breeds. 46 individuals from 25 breeds or strains were genotyped based on the Illumina Ovine 50K SNP array. Meanwhile, genetic variations among 482 individuals from 9 populations were genotyped with 10 microsatellites. In this study, we found high genetic polymorphisms for the microsatellites, while 7 loci in the Chinese superfine Merino strain （Xinjiang types） （CMS） and 5 loci in Gansu alpine superfine-wool sheep strain （GSS） groups were found deviated from Hardy-Weinberg equilibrium （HWE）. Genetic drift FsT=0.019 （P〈0.001） and high gene flows were detected in all the 7 fine-wool sheep populations. Phylogenetic analysis showed fine-wool sheep populations were clustered in a group independent from the Chinese indigenous breeds such that the 7 fine-wool sheep clustered distinct from Liangshan semifine-wool sheep （LS） and Hu sheep （HY） reflected by different population differentiation analyses. Overall, our findings suggested that all fine-wool sheep populations have close genetic relationship, which is consistent with their breeding progress. These populations, therefore, can be regarded as open-breeding populations with high levels of gene flows. Furthermore, the two superfine-wool strains, viz., CMS and GSS, might be formed by strong artificial selection and with frequent introduction of Australian Merino. Our results can assist in breeding of superfine-wool sheep and provide guidance for the cultivation of new fine-wool sheep breeds with different breeding objectives.展开更多
[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific reference...[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific references for the further study on the functions of KAP6.1 gene and molecular breeding of fine wool sheep. [Method] By using real-time fluorescence quantitative PCR, the expression map of KAP6.1 gene in various tissues was analyzed, and then PCR-SSCP technology, cloning and sequencing were adopted to analyze the polymorphism of KAP6.1 gene in 693 Chinese merino sheep （Xinjiang Junken type）, finally the correlation between KAPS. 1 gene and wool traits was discussed. [ Result] A high -level expression of KAP6.1 gene appeared in sheep skin, very significantly higher than that in muscle, small intestines, ovaries, hearts, lungs, livers, spleens, kidneys and rumen （ P 〈0.01 ）. In addition, there was C159T base substitution in KAF6.1 gene sequence, and analysis of the least squares model showed that the mean wool fleece weight of BB genotype at C159T site was significantly higher than that of AA and AB genotype （ P 〈0.05）, while there was no significant difference in average wool fiber diameter, curvature, length, clean fleece rate and density among hA, BB and AB genotype of KAP6.1 gene at C159T site （P〉0.05）. [Conclusion] KAP6.1 gene could be as the candidate gene of wool yield of sheep, and BB genotype could be used as the important molecular marker of fine wool sheep for high wool yield.展开更多
基金supported by the National Natural Science Foundation of China (31360543 and 31760655)the earmarked fund for the China Agriculture Research System (CARS-39)+1 种基金the Open Project of Key Laboratory of Genetics Breeding and Reproduction of Xinjiang Cashmere and Wool Sheep, China (2016D03017)the Postdoctoral Science Foundation, China (2017M623287)
文摘As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD)(P=0.04) and live weight (LW)(P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW)(P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P<0.05) than that of the fine wool (F) group. Moreover, it had a higher expression level (P<0.01) in skin tissues than in other tissues of Chinese Merino ewes. While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos. Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS).
基金sponsored by the Earmarked Fund for Modern China Wool & Cashmere Technology Research System (CARS-40-03)the National Natural Science Foundation for Young Scholars of China (31402057)Project support was provided by the ASTIP (Agricultural Science and Technology Innovation Program) for Genetic Resource and Breeding of Fine-Wool Sheep, Chinese Academy of Agricultural Sciences
文摘The aim of our present study was to construct genetic structure and relationships among Chinese fine-wool sheep breeds. 46 individuals from 25 breeds or strains were genotyped based on the Illumina Ovine 50K SNP array. Meanwhile, genetic variations among 482 individuals from 9 populations were genotyped with 10 microsatellites. In this study, we found high genetic polymorphisms for the microsatellites, while 7 loci in the Chinese superfine Merino strain （Xinjiang types） （CMS） and 5 loci in Gansu alpine superfine-wool sheep strain （GSS） groups were found deviated from Hardy-Weinberg equilibrium （HWE）. Genetic drift FsT=0.019 （P〈0.001） and high gene flows were detected in all the 7 fine-wool sheep populations. Phylogenetic analysis showed fine-wool sheep populations were clustered in a group independent from the Chinese indigenous breeds such that the 7 fine-wool sheep clustered distinct from Liangshan semifine-wool sheep （LS） and Hu sheep （HY） reflected by different population differentiation analyses. Overall, our findings suggested that all fine-wool sheep populations have close genetic relationship, which is consistent with their breeding progress. These populations, therefore, can be regarded as open-breeding populations with high levels of gene flows. Furthermore, the two superfine-wool strains, viz., CMS and GSS, might be formed by strong artificial selection and with frequent introduction of Australian Merino. Our results can assist in breeding of superfine-wool sheep and provide guidance for the cultivation of new fine-wool sheep breeds with different breeding objectives.
基金supported by Genetically Modified Organisms Breeding Major Projects ( 2009ZX08009-160B) Science and Technology Guidance Plan of Xinjiang Academy of Agricultural and Reclamation Sciences (YYD2010-8)+1 种基金 Science and Technology Key Project of Bintuan Agriculture (2009GG17,2011BA006) Doctoral Foundation of Bintuan (2011BB015)
文摘[ Objective] The study aimed to analyze the expression and polymorphism of KAP6.1 gene in various tissues of sheep, as well as the correlation between KAP6.1 gene and wool traits, so as to provide scientific references for the further study on the functions of KAP6.1 gene and molecular breeding of fine wool sheep. [Method] By using real-time fluorescence quantitative PCR, the expression map of KAP6.1 gene in various tissues was analyzed, and then PCR-SSCP technology, cloning and sequencing were adopted to analyze the polymorphism of KAP6.1 gene in 693 Chinese merino sheep （Xinjiang Junken type）, finally the correlation between KAPS. 1 gene and wool traits was discussed. [ Result] A high -level expression of KAP6.1 gene appeared in sheep skin, very significantly higher than that in muscle, small intestines, ovaries, hearts, lungs, livers, spleens, kidneys and rumen （ P 〈0.01 ）. In addition, there was C159T base substitution in KAF6.1 gene sequence, and analysis of the least squares model showed that the mean wool fleece weight of BB genotype at C159T site was significantly higher than that of AA and AB genotype （ P 〈0.05）, while there was no significant difference in average wool fiber diameter, curvature, length, clean fleece rate and density among hA, BB and AB genotype of KAP6.1 gene at C159T site （P〉0.05）. [Conclusion] KAP6.1 gene could be as the candidate gene of wool yield of sheep, and BB genotype could be used as the important molecular marker of fine wool sheep for high wool yield.
