Modern technology-based exploration of mechanism of traditional Chinese medicine in prevention and treatment of gastric cancer

This review comments on the article“To explore the mechanism of Yigong San anti-gastric cancer and immune regulation”.We are interested that the article applied network pharmacology and bioinformatics techniques to elucidate the mechanism of action of Yigong Sang,a traditional Chinese medicine(TCM),in the treatment of gastric cancer(GC).The mechanism of action of Yigong Sang in the treatment of GC has not yet been elucidated because it is composed of multiple Chinese medicines with multiple components and multiple targets.The emergence of network pharmacology and bioinformatics analysis helps explain the mechanism of action of TCM in preventing and treating GC,and provides a possibility for TCM to transform from empirical to evidence-based medicine.This is of great significance for the application of TCM in oncology,new drug development,formula optimization,and the improvement of clinical efficacy.

Inhibition of hepatitis B virus via selective apoptosis modulation by Chinese patent medicine Liuweiwuling Tablet

BACKGROUND Liuweiwuling Tablet(LWWL)is a Chinese patent medicine approved for the treatment of chronic inflammation caused by hepatitis B virus(HBV)infection.Previous studies have indicated an anti-HBV effect of LWWL,specifically in terms of antigen inhibition,but the underlying mechanism remains unclear.AIM To investigate the potential mechanism of action of LWWL against HBV.METHODS In vitro experiments utilized three HBV-replicating and three non-HBV-replicating cell lines.The in vivo experiment involved a hydrodynamic injectionmediated mouse model with HBV replication.Transcriptomics and metabolomics were used to investigate the underlying mechanisms of action of LWWL.RESULTS In HepG2.1403F cells,LWWL(0.8 mg/mL)exhibited inhibitory effects on HBV DNA,hepatitis B surface antigen and pregenomic RNA(pgRNA)at rates of 51.36%,24.74%and 50.74%,respectively.The inhibition rates of LWWL(0.8mg/mL)on pgRNA/covalently closed circular DNA in HepG2.1403F,HepG2.2.15 and HepG2.A64 cells were 47.78%,39.51%and 46.74%,respectively.Integration of transcriptomics and metabolomics showed that the anti-HBV effect of LWWL was primarily linked to pathways related to apoptosis(PI3K-AKT,CASP8-CASP3 and P53 pathways).Apoptosis flow analysis revealed that the apoptosis rate in the LWWL-treated group was significantly higher than in the control group(CG)among HBV-replicating cell lines,including HepG2.2.15(2.92%±1.01%vs 6.68%±2.04%,P<0.05),HepG2.A64(4.89%±1.28%vs 8.52%±0.50%,P<0.05)and HepG2.1403F(3.76%±1.40%vs 7.57%±1.35%,P<0.05)(CG vs LWWL-treated group).However,there were no significant differences in apoptosis rates between the non-HBV-replicating HepG2 cells(5.04%±0.74%vs 5.51%±1.57%,P>0.05),L02 cells(5.49%±0.80%vs 5.48%±1.01%,P>0.05)and LX2 cells(6.29%±1.54%vs 6.29%±0.88%,P>0.05).TUNEL staining revealed a significantly higher apoptosis rate in the LWWL-treated group than in the CG in the HBVreplicating mouse model,while no noticeable difference in apoptosis rates between the two groups was observed in the non-HBV-replicating mouse model.CONCLUSION Preliminary results suggest that LWWL exerts a potent inhibitory effect on wild-type and drug-resistant HBV,potentially involving selective regulation of apoptosis.These findings offer novel insights into the anti-HBV activities of LWWL and present a novel mechanism for the development of anti-HBV medications.

Research on Technology Development of Traditional Chinese Medicine Extraction Based on Patent Information

Objective To understand the development status of traditional Chinese medicine extraction technology,so as to provide a reference for enterprises and scientific research institutions to determine the direction of technological innovation.Besides,the government can formulate scientific and technological innovation strategies.Methods Based on patent information,statistical methods were applied to analyze the development of traditional Chinese medicine extraction technology.Then,the core technology was identified by constructing a patent citation network.Finally,the main path analysis method was applied to discover the technological evolution path.Results and Conclusion The extraction technology of traditional Chinese medicine is still in the growth stage.At present,its R&D is based on enterprises,supplemented by scientific research institutions.The core technologies are ultrasonic extraction and enzymatic extraction.The main path represented by enzymatic extraction technology reveals the evolution process of traditional Chinese medicine extraction technology.Based on this,relevant suggestions are put forward.Firstly,technological innovation cooperation should be strengthened to explore new models of achievement transformation and cooperation.Secondly,key enterprises of technology clusters should be cultivated to accelerate breakthroughs in core technologies.Thirdly,combined technologies can achieve high-efficiency enzyme-assisted extraction and open up new ways of extracting traditional Chinese medicine.Fourthly,traditional techniques should be improved to develop innovative modern techniques.

Study on Driving Technology Innovation Using Patent Management Mechanism

In order to improve the cigarette enterprise technology innovation and patent management status, the macro work should start from the four aspects of patent ideology introduction, patent organization design, perfecting patent system and implementation of specific measures, and attention should be attached to strengthen the cooperation of the cigarette enterprise management departments. On the other hand, Jn the microscopic work level, it should build the culture atmosphere of the entire tobacco industry respect intellectual property rights, actively cultivate the na- tional patent agents and patent engineers, and gradually promote the patent work to develop in the direction of specialization, self-independence, so as to construct a scientific mechanism for patent management, and enhance the core competitiveness of the tobacco industry.

Arrangement and analysis of processing technology of Anguo traditional Chinese medicine: Taking Chinese patent medicine making process as an example

Processing of traditional Chinese medicine is a traditional pharmaceutical technology in China.The processing technology and methods in different places have their own characteristics.Anguo County,known as Qizhou in ancient times for its well-developed pharmaceutical industry,is known as"millennium medicine capital"and"the world's first pharmaceutical market".It is one of China's famous distribution centers of medicinal materials.Its traditional medicinal material processing and processing technology has high popularity.However,Influenced by inheritance mode and mechanical modernization,A large number of processing techniques of traditional Chinese medicine are in danger of being lost.This article focused on the traditional manufacturing process of Anguo's Chinese patent medicine.Relying on the literature,we collected and organized the Chinese patent medicine of Anguo,analyzed its varieties,sources of prescriptions and processing technology,which improves the understanding of Anguo's traditional Chinese medicine processing technology and its cultural connotation and provides a research basis for its inheritance and development.

Integration of multi-omics in investigations on the mechanisms of action of Chinese herbal medicine interventions in metabolic diseases

The rapid development of bioinformatics has provided novel approaches and methods for exploring the mechanisms of disease treatment via Chinese herbal medicines.Compound Chinese herbal medicines formulas have complex compositions and are characterized by their multiple constituents and diverse array of biological targets.Therefore,the mechanisms of action of most compound Chinese herbal medicines formulas cannot be adequately explained using a single pathway.Omics technologies describe high-throughput-based analytical and detection techniques,which include transcriptomics,proteomics,and metabolomics and provide multilayer parameter information that can be integrated to characterize the overall relationships involved in the therapeutic effects of Chinese herbal medicines formulas.Through their combination with network biology and drug effect networks,omics technologies also enable investigations into the mechanisms of disease treatment in traditional Chinese medicine.The integration of multiple omics technologies is in line with the concept of holism in traditional Chinese medicine and provides an approach for combining modern science and technology with traditional Chinese medicine theories.In recent years,omics technologies have been widely used to elucidate the mechanisms of action of Chinese herbal medicines.The latest studies employing multi-omics integration for investigating the mechanisms of action of Chinese herbal medicines interventions in metabolic diseases have devoted greater attention to in-depth explorations of disease pathogenesis.This paper provides a review of the following multi-omics technologies,which are used in research on the treatment of common metabolic diseases(e.g.,type-2 diabetes mellitus,nonalcoholic fatty liver disease):network pharmacology combined with metabolomics,16S rRNA sequencing combined with transcriptomics,16S rRNA sequencing combined with metabolomics,and 16S rRNA sequencing combined with network pharmacology and metabolomics.

新质生产力赋能中医药传承创新发展的策略与思考

文章探讨了新质生产力在推动中医药传承创新发展方面的策略与思考。科技创新交叉融合突破所产生的新质生产力,为中医药领域带来了前所未有的发展机遇。文章探讨了新质生产力在医药领域的潜力和发展方向,强调了其在推动中医药传承创新中的重要作用;分析了现代信息技术对中医药传承创新发展的影响,重点介绍了中药材大数据云服务平台、人工智能赋能、先进设备智能制造、大数据超算以及中医药循证研究证据库等方面的应用;展望了人工智能大模型在医药领域的发展方向,为未来中医药传承创新发展提供了新的视角和思路。

五育融合视域下中成药学课程教学的实践

中成药学涉及中医学、中药学等多门学科的交叉渗透和综合运用。文章通过分析中成药学实施“五育融合”的现状,探求该课程五育融合的契合点,并结合课程教学目标提出“五育融合”的教学策略,激发学生五育,发展其自身的内驱力,实现德智体美劳的渗透融合,有利于培养出适应中成药行业发展需求的应用型、复合型人才,也为相关专业课程教学改革与创新提供思路。

兽用中药制剂工艺的传承与创新

为了推动兽用中药制剂工艺的传承与创新发展,本文在总结中兽医方剂传承基础上,研究兽用中药粉碎与超微粉碎、提取等现代工艺发展,剖析中药制剂剂型定位及生产投料存在的问题,探索兽用中药制剂工艺传承创新导向和途径,对《中国兽药典》制剂通则提出修改建议,为兽用中药制剂研究和2025年版《中国兽药典》编撰提供参考。

中医药产业技术创新联盟的构建动机及运行机制研究——以江西省为例

基于中医药在改善民生、转变经济发展方式、弘扬中华文化、建设生态文明等国家大局中日益凸显的作用,结合产业技术创新联盟在推动产业发展、突破关键共性技术这一显著特点,提出中医药产业技术创新联盟的内涵,分析联盟构建动机。以江西省为例,从全生命周期角度提出中医药产业技术创新联盟的运行机制,保障联盟的稳定发展。

4种治疗糖尿病周围神经病变补气活血类中成药的卫生技术评估

目的为临床选择糖尿病周围神经病变(DPN)治疗用补气活血类中成药提供参考。方法参考《医疗机构中成药遴选专家共识(第一版)》,结合临床实际,制订药物卫生技术评估细则,参考药品说明书、临床指南和文献,对4种中成药的安全性、有效性、经济性、适宜性、创新性、可及性、传承性进行评分,并计算总分。结果通心络胶囊、木丹颗粒、糖脉康颗粒、芪蛭降糖胶囊的评分分别为74.5分、72分、64.5分、57分。结论临床在选择治疗DPN的补气活血类中成药时,相对于糖脉康颗粒、芪蛭降糖胶囊,建议优先使用木丹颗粒、通心络胶囊。

中医药科技创新基础研究重点方向思考

本文围绕用现代科学解读中医药学原理、发挥中医药防病治病的独特优势与作用、提高中药全链条创新能力,提出了中医药科技创新基础研究的重点方向。对每个重点方向的来源依据、研究进展、研究重点及相关科学问题进行阐释,为中医药现代化基础研究提供参考。

数字技术在中医药科技创新中的应用及未来发展趋势

数字化是中医药发展的必然选择,是新形势下中医药科技创新的新动能。随着我国数字经济的不断发展,数字化对中医药科技创新更加重要。系统梳理了当前中医药领域数字化转型发展的现状,从数字技术在中医药科技创新中的应用现状、未来发展趋势、面临的挑战与对策建议三大方面论述了数字技术在中医药科技创新中的地位与作用。在当下和今后很长一段时间,数字化将持续大力推动中医药科技创新,促进中医药科学技术快速发展。

中医药院校研究生思想政治教育路径探析

开展正确、规范且符合时代特色与专业特色的研究生思想政治教育,是高校在培养具备良好政治素养的高学历人才过程中不可松懈的环节.中医药蕴含着中华传统文化的精髓,中医药研究生的思想政治教育也有别于其他专业,具有独特性.将中医药文化中的哲学思想与当代民族文化精神及社会主义核心价值观巧妙衔接,对于创新具有浓厚中医药特色的中医药研究生思政教育机制具有重要意义.系统剖析当前中医药研究生思想政治教育中面临的挑战与存在的不足,并结合多年来在工作中的实践经验,探索出中医药研究生思想政治教育在机制创新上的可行性路径.

基于政策工具的我国中医药科技创新政策文本分析

目的:分析我国中医药科技创新政策的侧重点与不足,为后续政策优化提供参考。方法:检索2007年以来国家层面发布的中医药科技创新政策文本,采用二维分析框架对其进行量化分析。结果:27份政策中,X维度供给型、环境型、需求型政策工具使用比例分别为48.98%、39.29%、11.73%;Y维度推动中医药科技创新和成果转化占比最高(29.03%);推动中西医结合发展使用最少(1.08%)。结论:政策工具结构性失衡,政策目标分布差异性显著,政策目标内部政策工具使用失衡。建议:优化政策工具内部结构,完善政策目标结构,推动政策工具在中医药科技创新政策目标上的动态组合运用。

多组学整合策略在中药抗抑郁机制研究中的应用

抑郁症是一种常见的精神和心理疾病,发病机制复杂,中医辨证论治治疗抑郁症具有因人而异、因病而异的独特优势。随着分子生物学技术快速发展,多组学技术在中药有效成分抗抑郁效果的分析中得到广泛应用。文章系统介绍了多组学的核心技术,重点综述了近年来基因组学、蛋白质组学、转录组学、代谢组学和多组学整合技术在中药抗抑郁作用机制研究中的思路、方法和进展,为推进多组学技术在中药治疗抑郁症中的应用提供参考。

贵州太子参生产全程机械化技术现状及对策

太子参是贵州的道地药材,经过20年的发展,形成了集约化、规范化的种植模式,种植面积全国第一。但贵州太子参多种植于丘陵山地,受地形地貌和发展基础的影响,生产作业费时费力、效率低、机械化难度大。通过查阅文献及实地考察,结合贵州太子参的典型种植农艺与初加工工艺,简述了太子参全链条生产技术,分析了耕、种、管、收、初加工5个环节的机械化技术现状,指出太子参生产机械化存在的主要问题。基于此,提出太子参生产全程机械化对策建议,包括推行标准化种植,推动农机农艺融合及种植区宜机化改造;加大山地太子参块根播种、收获、去尾、分级等关键技术的研发力度,加强农机技术型人才培养,加快轻简型太子参播种机和收获机的研制,升级改造已有初加工机械设备,形成适度的初加工生产线。分析结果可为贵州太子参可持续性发展提供参考。

治疗白癜风中药复方专利的用药规律及多途径作用机制研究

目的探讨治疗白癜风中药复方专利的用药规律及作用机制,为白癜风中药新药开发与专利申报工作提供思路。方法借助中国专利公布公告网站、国家知识基础设施数据库、中国知识产权网(CNIPR)专利信息服务平台、佰腾(Baiten)专利平台分别检索建库至2023年9月30日治疗白癜风的中药复方专利,采用Microsoft Excel软件、中医传承计算平台(TCMICS)V3.0建立数据库并进行内服、外用专利药物频数统计、关联规则及聚类分析,并进一步通过中药系统药理数据库与分析平台(TCMSP)、中医药证候关联数据库(SymMap)、人类基因综合分析数据库(GeneCards)、蛋白质资源数据库(UniProt)、STRING、药物综合数据库(PDB)等数据库及RStudio4.2.2、Cytoscape3.9.1等软件探讨内服专利核心高频药物组合治疗白癜风的作用靶点及机制,利用AutoDock Vina软件进行分子对接验证。结果纳入335项内服专利,189项外用专利。内服与外用专利用药规律较为相似,高频药物均为蒺藜、补骨脂、当归、何首乌、红花、白芷等;关键功效均以补虚、活血化瘀为主;皆以性温、寒、平,味甘、苦、辛,归肝、脾、心、肾经为主。内服专利中高频药对有川芎-当归、黄芪-当归等,外用专利中高频药对有乌梅-补骨脂、菟丝子-补骨脂等;内服与外用专利的聚类核心药物均有补骨脂、蒺藜、当归、何首乌、白芷、红花等高频药物。内服专利的治疗关键靶点为白蛋白、蛋白激酶B1、胱天蛋白酶3等基因,主要活性成分可能为槲皮素、山柰酚、木犀草素等,治疗机制可能与糖基化终产物/糖基化终产物受体、肿瘤坏死因子信号通路、磷脂酰肌醇3-激酶/蛋白激酶B等信号通路等相关,并参与血脂与动脉粥样硬化、人类巨细胞病毒感染等过程。分子对接结果证实中药成分与关键受体蛋白具有对接活性。结论治疗白癜风的内服与外用中药复方专利在用药、性味、归经方面具有相似性,提示临床可从补益肝肾、活血化瘀等方面入手改善患者的白蛋白、白细胞介素、肿瘤坏死因子等通路及指标。本研究也为治疗白癜风的新药研发、专利保护及临床遣方用药提供了参考。

中药安全性评价理论创新与技术突破:病证毒理学

目的提出病证毒理学理论及方法体系,皆在促进病证毒理学在中药新药开发、中药临床安全性评价以及中药安全精准用药对策制定方面的科学、合理和高效运用,促进中医药事业健康可持续发展。方法结合病证毒理学实践运用情况,全面定义病证毒理学的外延和内涵,并系统梳理基于病证毒理学的中药安全性研究新理论、新方法和新发现。结果中药毒性主要分为固有毒性和特异质毒性2种类型,由于特异质毒性具有偶发性,易感性和难预测等特点,常规毒理学模式和方法难以评价,为此本团队传承创新中医“有故无殒”毒效思想,2016年首次提出病证毒理学理论和方法,皆在突破常规毒理学模式和方法局限性,科学厘清遗传、体质、疾病和证候等机体因素对中药安全性的影响规律和主次关系,解决中药特异质毒性评价难题。近年来,本团队在不断完善和优化病证毒理学理论和方法体系基础上,以临床真实世界和体内外病证易感模型为主要评价模式,开展了何首乌、补骨脂和淫羊藿等中药及及其相关制剂特异质毒性的易感人发现识别、成因机制解析及风险防控对策研究,破解了系列传统“无毒”中药安全性评价及风险防控难题。结论病证毒理学不仅可以用于特异质毒性中药的易感人群识别、成因机制解析以及风险防控对策研究,还可以用于固有毒性中药的“病(证)-量-时-毒-效”关系以及安全治疗窗研究。病证毒理学创新发展了中药毒理学理论和方法,为中药安全精准用药提供了奠基性理论和技术支撑。

我国中医药科技创新面临的挑战及发展举措

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