Explants,namely the seeds (Cymbidium faberi) and buds (Cymbidium goeringii) of local Hunan orchids were disinfected with 75% ethanol, 0.1% mercuric chloride,and 10% sodium hypochlorite in six different disinfection tr...Explants,namely the seeds (Cymbidium faberi) and buds (Cymbidium goeringii) of local Hunan orchids were disinfected with 75% ethanol, 0.1% mercuric chloride,and 10% sodium hypochlorite in six different disinfection treatments. Results showed that The treatment with 75% ethanol (contact time: 45 s) and 0.1% mercuric chloride (contact time: 8 min) proved to be the most effective one in disinfecting the explants of Cymbidium faberi seeds.The combination of 75% ethanol (contact time: 45 s) and 0.1% mercuric chloride (contact time: 5 min) provided the optimal disinfection effects when disinfecting Cymbidium goeringii buds.In general, the disinfection effect of mercuric chloride was found to be superior to that of sodium hypochlorite,but care should be taken with the disinfection time to avoid damage to the explants.展开更多
文摘Explants,namely the seeds (Cymbidium faberi) and buds (Cymbidium goeringii) of local Hunan orchids were disinfected with 75% ethanol, 0.1% mercuric chloride,and 10% sodium hypochlorite in six different disinfection treatments. Results showed that The treatment with 75% ethanol (contact time: 45 s) and 0.1% mercuric chloride (contact time: 8 min) proved to be the most effective one in disinfecting the explants of Cymbidium faberi seeds.The combination of 75% ethanol (contact time: 45 s) and 0.1% mercuric chloride (contact time: 5 min) provided the optimal disinfection effects when disinfecting Cymbidium goeringii buds.In general, the disinfection effect of mercuric chloride was found to be superior to that of sodium hypochlorite,but care should be taken with the disinfection time to avoid damage to the explants.
