It was rarely reported about strawberry vein banding virus(SVBV)genome sequence in China and most countries worldwide.In this work,we determined the complete genome sequences of two SVBV isolates in China,designated S...It was rarely reported about strawberry vein banding virus(SVBV)genome sequence in China and most countries worldwide.In this work,we determined the complete genome sequences of two SVBV isolates in China,designated SVBV-AH and SVBV-BJ,that were obtained from naturally infected strawberry samples from Anhui province and Beijing city of China,respectively.The complete genomes of SVBV-AH and SVBV-BJ were 7,862 nucleotides(nts)and 7,863 nts long,respectively,and both constituted with seven genes typical of the caulimoviruses.Alignment of complete nucleotide sequences showed that SVBV-AH and SVBV-BJ shared a significant nucleotide sequence identity of 97.7%of each other and had 85.7%and 86.0%sequence identity related to SVBV from the United States(SVBV-US),respectively.Phylogenetic trees,based on the alignment of complete nucleotide sequences and amino acid sequences of Coat Protein(CP),both showed that SVBV-AH and SVBV-BJ clustered into one branch with all the other SVBV isolates,and other species of caulimoviruses clustered into another tree branch.It illustrated that all the SVBV isolates had an extremely high relationship but had a distant relationship with other species of caulimoviruses.We further confirmed that SVBV-AH infectious clone could cause similar symptoms to SVBVinfected in strawberry under natural conditions.Taken together,our study provided valuable information to elucidate the origin and dissemination of SVBV Chinese isolates,meanwhile providing the necessary vector for studying the gene functions of strawberry.展开更多
在日光温室的后墙上,采用管道无土栽培方式进行蔬菜或草莓生产,可以提高温室空间利用率和作物种植量,但可能会出现因为管道和植物的挡光而减少后墙蓄热、降低冬季温室温度的问题。为此,通过冬季连续31d的温度监测,在3种典型气象(晴天、...在日光温室的后墙上,采用管道无土栽培方式进行蔬菜或草莓生产,可以提高温室空间利用率和作物种植量,但可能会出现因为管道和植物的挡光而减少后墙蓄热、降低冬季温室温度的问题。为此,通过冬季连续31d的温度监测,在3种典型气象(晴天、阴天、雪天)条件下,对比分析了有后墙立体基质栽培的日光温室(solargreenhouse with equipment,ESG)和无后墙立体栽培的日光温室(solar greenhouse with no equipment,NSG)温度环境的变化。监测结果表明,ESG的月平均气温较NSG高0.84℃,其中最大日温差为2.22℃,最小日温差为0.14℃。晴天条件下,ESG的日平均冠层温度和1.5m高度处的空气温度分别是12.72和13.04℃,NSG分别是10.68和11.04℃;ESG的冠层温度最低值是4.68℃,而NSG最低值是4.10℃。可见,ESG较NSG的气温要略高一些;阴天和雪天条件下,2种温室内的温度环境无显著差别。因此,利用日光温室后墙进行立体基质栽培草莓,不但没有降低反而提高了冬季温室内的温度,是一种可行、值得推广应用的温室高效栽培技术。展开更多
基金the National Natural Science Foundation of China(Nos.32072386 and 31801700)the Key Research and Development Project of Anhui Province(202004a06020013)the Anhui Postdoctoral Fund(2019B360).
文摘It was rarely reported about strawberry vein banding virus(SVBV)genome sequence in China and most countries worldwide.In this work,we determined the complete genome sequences of two SVBV isolates in China,designated SVBV-AH and SVBV-BJ,that were obtained from naturally infected strawberry samples from Anhui province and Beijing city of China,respectively.The complete genomes of SVBV-AH and SVBV-BJ were 7,862 nucleotides(nts)and 7,863 nts long,respectively,and both constituted with seven genes typical of the caulimoviruses.Alignment of complete nucleotide sequences showed that SVBV-AH and SVBV-BJ shared a significant nucleotide sequence identity of 97.7%of each other and had 85.7%and 86.0%sequence identity related to SVBV from the United States(SVBV-US),respectively.Phylogenetic trees,based on the alignment of complete nucleotide sequences and amino acid sequences of Coat Protein(CP),both showed that SVBV-AH and SVBV-BJ clustered into one branch with all the other SVBV isolates,and other species of caulimoviruses clustered into another tree branch.It illustrated that all the SVBV isolates had an extremely high relationship but had a distant relationship with other species of caulimoviruses.We further confirmed that SVBV-AH infectious clone could cause similar symptoms to SVBVinfected in strawberry under natural conditions.Taken together,our study provided valuable information to elucidate the origin and dissemination of SVBV Chinese isolates,meanwhile providing the necessary vector for studying the gene functions of strawberry.
文摘在日光温室的后墙上,采用管道无土栽培方式进行蔬菜或草莓生产,可以提高温室空间利用率和作物种植量,但可能会出现因为管道和植物的挡光而减少后墙蓄热、降低冬季温室温度的问题。为此,通过冬季连续31d的温度监测,在3种典型气象(晴天、阴天、雪天)条件下,对比分析了有后墙立体基质栽培的日光温室(solargreenhouse with equipment,ESG)和无后墙立体栽培的日光温室(solar greenhouse with no equipment,NSG)温度环境的变化。监测结果表明,ESG的月平均气温较NSG高0.84℃,其中最大日温差为2.22℃,最小日温差为0.14℃。晴天条件下,ESG的日平均冠层温度和1.5m高度处的空气温度分别是12.72和13.04℃,NSG分别是10.68和11.04℃;ESG的冠层温度最低值是4.68℃,而NSG最低值是4.10℃。可见,ESG较NSG的气温要略高一些;阴天和雪天条件下,2种温室内的温度环境无显著差别。因此,利用日光温室后墙进行立体基质栽培草莓,不但没有降低反而提高了冬季温室内的温度,是一种可行、值得推广应用的温室高效栽培技术。
