Uncinula necator is a worldwide serious fungus disease causing annual heavy lost on grapevine production. In order to get more informations on defense related EST sequences and help breeding program, we constructed an...Uncinula necator is a worldwide serious fungus disease causing annual heavy lost on grapevine production. In order to get more informations on defense related EST sequences and help breeding program, we constructed and characterized a suppression subtractive hybridization cDNA library with artificially inoculated leaves and control Chinese wild Vitis pseudoreticulata clone Baihe-35-1, which is highly resistant to powdery mildew. In the library, the length of 58 EST fragments known as putative functions varied from 130 to 800 bp, and 60% of the ESTs exhibited high similarity to known sequences in database of GenBank with BLASTX analysis. These genes were involved in stress/defense response, detoxification, signal transduction, disease defense, and etc., and 14 ESTs remained unknown or hypothetical proteins, which may be new genes. The experiment provided an important basis for studying the disease-resistance mechanism and obtaining the genes for the aim of improving grapevine powdery mildew resistance.展开更多
With F1 individuals of the cross combination 88-110 of 83-4-96 ( V. quinquangularis Rehd.) X Muscat Rose ( V. vinifera L.), the RAPD marker OPC15-1300 linked to ripe rot ( Gloeosporium fructi-genum Berk.) resistance g...With F1 individuals of the cross combination 88-110 of 83-4-96 ( V. quinquangularis Rehd.) X Muscat Rose ( V. vinifera L.), the RAPD marker OPC15-1300 linked to ripe rot ( Gloeosporium fructi-genum Berk.) resistance genes in Chinese wild Vitis was gained using bulked segregation analysis (BS A). And it was found that OPC15-1300 could be hereditary from the resistant parent (83-4-96) after the marker was tested in 50 Fj plants of the cross combination 88-110, 32 accessions of 8 Chinese wild Vitis species and 14 cultivars of V. vinifera L. Also, it has provided a solid basis for molecular marker-assisted selection (MAS) and for possibly cloning disease resistance genes in the future.展开更多
基金funded by the National Natural Science Foundation of China(30571280, 30771493)the Transcentury Talent-Training Program of Ministry of Education of China and the National 863 Project of China(2007AA10Z182)
文摘Uncinula necator is a worldwide serious fungus disease causing annual heavy lost on grapevine production. In order to get more informations on defense related EST sequences and help breeding program, we constructed and characterized a suppression subtractive hybridization cDNA library with artificially inoculated leaves and control Chinese wild Vitis pseudoreticulata clone Baihe-35-1, which is highly resistant to powdery mildew. In the library, the length of 58 EST fragments known as putative functions varied from 130 to 800 bp, and 60% of the ESTs exhibited high similarity to known sequences in database of GenBank with BLASTX analysis. These genes were involved in stress/defense response, detoxification, signal transduction, disease defense, and etc., and 14 ESTs remained unknown or hypothetical proteins, which may be new genes. The experiment provided an important basis for studying the disease-resistance mechanism and obtaining the genes for the aim of improving grapevine powdery mildew resistance.
文摘With F1 individuals of the cross combination 88-110 of 83-4-96 ( V. quinquangularis Rehd.) X Muscat Rose ( V. vinifera L.), the RAPD marker OPC15-1300 linked to ripe rot ( Gloeosporium fructi-genum Berk.) resistance genes in Chinese wild Vitis was gained using bulked segregation analysis (BS A). And it was found that OPC15-1300 could be hereditary from the resistant parent (83-4-96) after the marker was tested in 50 Fj plants of the cross combination 88-110, 32 accessions of 8 Chinese wild Vitis species and 14 cultivars of V. vinifera L. Also, it has provided a solid basis for molecular marker-assisted selection (MAS) and for possibly cloning disease resistance genes in the future.
