期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
The EST Analysis of A Suppressive Subtraction cDNA Library of Chinese Wild Vitis pseudoreticulata Inoculated with Uncinula necator 被引量:5
1
作者 SHI Jiang-li, WANG Yue-jin, ZHU Zi-guo and ZHANG Chao-hong Key Laboratory of Horticultural Plant Germplasm Utilization in Northwest China, Ministry of Agriculture/Shaanxi Key Laboratory of Molecular Biology for Agriculture/College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China 《Agricultural Sciences in China》 CSCD 2010年第2期233-241,共9页
Uncinula necator is a worldwide serious fungus disease causing annual heavy lost on grapevine production. In order to get more informations on defense related EST sequences and help breeding program, we constructed an... Uncinula necator is a worldwide serious fungus disease causing annual heavy lost on grapevine production. In order to get more informations on defense related EST sequences and help breeding program, we constructed and characterized a suppression subtractive hybridization cDNA library with artificially inoculated leaves and control Chinese wild Vitis pseudoreticulata clone Baihe-35-1, which is highly resistant to powdery mildew. In the library, the length of 58 EST fragments known as putative functions varied from 130 to 800 bp, and 60% of the ESTs exhibited high similarity to known sequences in database of GenBank with BLASTX analysis. These genes were involved in stress/defense response, detoxification, signal transduction, disease defense, and etc., and 14 ESTs remained unknown or hypothetical proteins, which may be new genes. The experiment provided an important basis for studying the disease-resistance mechanism and obtaining the genes for the aim of improving grapevine powdery mildew resistance. 展开更多
关键词 chinese wild vitis pseudoreticulata disease resistance SSH cDNA library EST sequence
下载PDF
Detecting RAPD Markers Linked to Ripe Rot Resistance Genes in Chinese Wild Vitis
2
作者 WANG Yue-jin, XU Yan, ZHANG Jian-xia, ZHOU Peng and WAN Yi-zhen( College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100 , P . R . China National Key Laboratory of Biotechnology for Tropical Crops , CATAS, Haikou 571101 , P.R.China) 《Agricultural Sciences in China》 CAS CSCD 2002年第5期572-576,共5页
With F1 individuals of the cross combination 88-110 of 83-4-96 ( V. quinquangularis Rehd.) X Muscat Rose ( V. vinifera L.), the RAPD marker OPC15-1300 linked to ripe rot ( Gloeosporium fructi-genum Berk.) resistance g... With F1 individuals of the cross combination 88-110 of 83-4-96 ( V. quinquangularis Rehd.) X Muscat Rose ( V. vinifera L.), the RAPD marker OPC15-1300 linked to ripe rot ( Gloeosporium fructi-genum Berk.) resistance genes in Chinese wild Vitis was gained using bulked segregation analysis (BS A). And it was found that OPC15-1300 could be hereditary from the resistant parent (83-4-96) after the marker was tested in 50 Fj plants of the cross combination 88-110, 32 accessions of 8 Chinese wild Vitis species and 14 cultivars of V. vinifera L. Also, it has provided a solid basis for molecular marker-assisted selection (MAS) and for possibly cloning disease resistance genes in the future. 展开更多
关键词 chinese wild vitis Ripe rot resistance RAPD marker
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部