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Synthesis and Characterization of Nonionic Waterborne Polyurethane and Application to Wool Fabric Finishing
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作者 殷映婷 FENG Mao +1 位作者 姚金波 牛家嵘 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2024年第2期497-505,共9页
A nonionic waterborne polyurethane(WPU) was synthesized by the self-emulsification method using polyether diol(N220),isophorone diisocyanate(IPDI),trimethylolpropane poly(ethylene glycol monomethyl ether)(N120),1,4-bu... A nonionic waterborne polyurethane(WPU) was synthesized by the self-emulsification method using polyether diol(N220),isophorone diisocyanate(IPDI),trimethylolpropane poly(ethylene glycol monomethyl ether)(N120),1,4-butanediol(BDO) and trimethylolpropane(TMP) as the main materials.The effects of the NCO/OH ratio on the emulsion and film properties of NWPU were explored.The experimental results show that the NWPU prepared at an NCO/OH ratio of 1.1 has good emulsion stability and easy film formation,and the resultant film was elastic,soft,and transparent.The sample was used for wool finishing and the application performance was evaluated.When the NWPU dosage reached 40 g·L^(-1),the fabric area felt shrinkage rate reduced from 8.97% to 4.75%,the pilling rating raised from grade 2-3 to grade 4,and the whiteness value only decreased by 3.87%. 展开更多
关键词 waterborne polyurethane NONIONIC wool finishing ANTI-PILLING anti-shrinking
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Enhancing Thermal Performance of Building Envelopes Using Hemp Wool and Wood Wool with Phase Change Materials
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作者 Salma Kouzzi Mouniba Redah +2 位作者 Souad Morsli Mohammed El Ganaoui Mohammed Lhassane Lahlaouti 《Fluid Dynamics & Materials Processing》 EI 2024年第12期2741-2755,共15页
This study investigates the potential for enhancing the thermal performance of external walls insulation in warmer climates through the combination of phase change materials(PCMs)and bio-based materials,specifically h... This study investigates the potential for enhancing the thermal performance of external walls insulation in warmer climates through the combination of phase change materials(PCMs)and bio-based materials,specifically hemp wool and wood wool.Experimental tests using the heat flow method(HFM),and numerical simulations with ANSYS Fluent software were conducted to assess the dynamic thermal distribution and fluid-mechanical aspects of phase change materials(PCMs)within composite walls.The results demonstrate a notable reduction in peak indoor temperatures,achieving a 58%reduction with hemp wool with a close 40%reduction with wood wool when combined with PCMs.Fluid-mechanical analysis indicates that PCMs act as efficient indoor temperature regulators by storing excess heat during hot periods and releasing it later during phase transitions.Furthermore,the homogeneous distribution of the liquid fraction and natural convection during phase change contribute significantly to the improvement in heat transfer rates,resulting in a 96%reduction compared to hemp wool and wood wool without PCMs. 展开更多
关键词 Bio-based materials phase change material(PCM) heat flow method thermal performance external wall insulation hemp wool wood wool ANSYS Fluent software dynamic thermal distribution fluid-mechanical analysis
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Will quick-drying wool be the next outdoor winner?
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作者 Zhong Mengxia 《China Textile》 2024年第6期56-57,共2页
With the improvement of people's living standards and the strengthening of health awareness,outdoor sports and its supporting clothing have become a fashionable lifestyle.Consumers are paying more and more attenti... With the improvement of people's living standards and the strengthening of health awareness,outdoor sports and its supporting clothing have become a fashionable lifestyle.Consumers are paying more and more attention to the comfort and fashionable appearance of clothing,and this change in demand is blurring the lines of application of professional clothing and equipment,and outdoor clothing is no longer limited to specific sports scenarios. 展开更多
关键词 wool strengthening SUPPORTING
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Sustainable art exhibition RETURN visualizes wool's biodegradable properties
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作者 Zhong Mengxia 《China Textile》 2024年第2期26-27,共2页
In early April,The Woolmark Company,the authoritative wool textiles organization,presented RETURN,a sustainable art exhibition at R130,a buyer shop during Shanghai Fashion Week,where three pieces of naturally degraded... In early April,The Woolmark Company,the authoritative wool textiles organization,presented RETURN,a sustainable art exhibition at R130,a buyer shop during Shanghai Fashion Week,where three pieces of naturally degraded wool fabrics were on display for the first time in China.The exhibition visualised the biodegradation process of this natural fabric,leading people to experience the natural charm of wool through art and natural beauty. 展开更多
关键词 wool EXHIBITION URN
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彭波半细毛羊冷冻保存睾丸组织支持细胞分离、纯化及鉴定
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作者 刘海霞 王健 +4 位作者 平措班旦 朱爱文 德庆卓嘎 王军 格桑加措 《中国畜牧兽医》 北大核心 2025年第1期249-257,共9页
[目的]睾丸支持细胞(Sertoli cells, SCs)位于生精小管内上皮,为不同分化阶段雄性生殖细胞发育提供物理构架和能量物质支持,对精子发生过程产生至关重要的作用。试验旨在研究新鲜绵羊睾丸组织样品获取不便的情况下,从冷冻保存的彭波半... [目的]睾丸支持细胞(Sertoli cells, SCs)位于生精小管内上皮,为不同分化阶段雄性生殖细胞发育提供物理构架和能量物质支持,对精子发生过程产生至关重要的作用。试验旨在研究新鲜绵羊睾丸组织样品获取不便的情况下,从冷冻保存的彭波半细毛羊睾丸组织分离支持细胞的技术方法。[方法]将冷冻保存液预处理后冻存的彭波半细毛羊睾丸组织进行复苏,采用苏木精-伊红(HE)染色检测复苏后睾丸组织形态,利用组合酶消化法、差速贴壁法进行睾丸组织支持细胞分离纯化培养,观察细胞生长规律,绘制生长曲线,采用RT-PCR和免疫荧光染色(IF)技术进行睾丸支持细胞特异性基因鉴定。[结果]冻存复苏睾丸组织曲细精索及睾丸间质保存完好。组合酶消化后能获得满足试验需要的生精上皮细胞混悬液,分离培养2~4 h后支持细胞贴壁,形态呈梭形或不规则多边形,培养3~4 d后汇合,其中培养1~2 d生长速度缓慢,培养3~6 d进入对数期,增殖速度加快,培养7~8 d后增殖速度下降。RT-PCR检测显示,GNDF、WT1、ABP、SOX9基因均在彭波半细毛羊睾丸支持细胞中特异性表达;免疫荧光染色显示,特异性抗体GATA4、Vimentin免疫阳性。[结论]经冻存液预处理的冷冻保存睾丸组织复苏后,可分离出满足试验条件的原代支持细胞,应用于科学试验研究。 展开更多
关键词 彭波半细毛羊 冷冻保存 睾丸支持细胞 分离鉴定 细胞培养
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ZIF-67/羊毛织物制备及对活性蓝222吸附性能
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作者 高文超 刘秀明 +1 位作者 李秋瑾 房宽峻 《针织工业》 北大核心 2025年第1期54-58,共5页
ZIF-67是Co2+和2-甲基咪唑桥连形成的纳米材料,用于污染物的吸附分离,但粉末状ZIF-67存在静电作用强、在液相中难分离和回收等问题。为解决以上问题,采用羊毛织物作为基底材料负载ZIF-67颗粒,用于印染废水中活性染料的吸附。通过原位生... ZIF-67是Co2+和2-甲基咪唑桥连形成的纳米材料,用于污染物的吸附分离,但粉末状ZIF-67存在静电作用强、在液相中难分离和回收等问题。为解决以上问题,采用羊毛织物作为基底材料负载ZIF-67颗粒,用于印染废水中活性染料的吸附。通过原位生长法成功制备ZIF-67/羊毛织物(ZW),进而探究ZW对活性蓝222的吸附性能。结果表明,当延长吸附时间、增加ZW用量、降低Na Cl浓度和p H值时,ZW均可表现出对活性蓝222较好的吸附性能。动力学试验测得ZW对活性蓝222的吸附量为3.30 mg/g。其准二级动力学、Langmuir型吸附等温线和热力学模型等说明活性蓝222以单分子层沉积的形式吸附在ZW表面。 展开更多
关键词 ZIF-67 羊毛织物 活性染料 吸附 吸附模型
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蛋白酶高效催化体系构建及其对羊毛角蛋白的降解
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作者 段志欣 王乐 +2 位作者 刘丹 安欣冉 卢建军 《毛纺科技》 北大核心 2025年第1期48-54,共7页
角蛋白内二硫键结构严重阻碍蛋白酶对其高效降解,为提高酶对角蛋白的降解效率,分别构建了由蛋白酶Savinase 16L与有机膦化合物LKZ-610、β-巯基乙醇、亚硫酸氢钠、L-半胱氨酸组成的4种催化体系。以羊毛鳞片角蛋白为研究对象,采用5,5’-... 角蛋白内二硫键结构严重阻碍蛋白酶对其高效降解,为提高酶对角蛋白的降解效率,分别构建了由蛋白酶Savinase 16L与有机膦化合物LKZ-610、β-巯基乙醇、亚硫酸氢钠、L-半胱氨酸组成的4种催化体系。以羊毛鳞片角蛋白为研究对象,采用5,5’-二硫代双(2-硝基苯甲酸)(DTNB)模拟反应、羊毛表面巯基测定及阿尔瓦登反应研究不同二硫键还原剂与二硫键的反应性,通过紫外可见分光光度计、扫描电子显微镜及X射线衍射仪等分析了催化体系对羊毛鳞片角蛋白的降解行为。结果表明:所选取的4种还原剂均可断开二硫键,其中LKZ-610可瞬时打开模拟物DTNB中98.12%的二硫键,其处理后的羊毛表面巯基含量达13.64μmol/g。对比不同催化体系对羊毛鳞片角蛋白的降解效果,优选出由LKZ-610与蛋白酶Savinase 16L组成的高效催化体系,其可在50 min内完全降解羊毛鳞片层,处理后羊毛织物质量损失率达35.81%,结晶指数由20.67%显著降低至4.25%。 展开更多
关键词 蛋白酶 羊毛 二硫键 角蛋白 降解 还原
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Differential Expression of Immune Genes between Body Side Skin and Groin Skin of Aohan Fine Wool Sheep
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作者 赵金山 李和刚 +2 位作者 刘开东 柳楠 李金泉 《Agricultural Science & Technology》 CAS 2012年第12期2475-2479,共5页
[Objective] To get major genes for wool traits regulation from immune genes. [Methods] Microarray technology was used to detect differentially expressed immune genes between body side skin (more wool growing) and gr... [Objective] To get major genes for wool traits regulation from immune genes. [Methods] Microarray technology was used to detect differentially expressed immune genes between body side skin (more wool growing) and groin skin (no wool growing) of Aohan fine wool sheep. [Results] 46 immune genes (fold change 〉2.0) were identified and classified, and then 6 of which were selected for QPCR confir- mation. The degree of consistency of the QPCR and microarray results was 66.67%, [Conclusion] Immune privilege may participate in wool growth regulation. 展开更多
关键词 Hair follicle Immune gene Differential expression Aohan fine wool sheep
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生物基偶联玫瑰红纳米光敏剂及其光动力抗菌织物
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作者 陈彧彦 吕子豪 +2 位作者 郑梦姣 光晓翠 李蔚 《印染》 北大核心 2025年第1期43-48,共6页
为解决光敏剂在蛋白质纤维应用上效能不足的问题,通过偶联壳聚糖(CS)和孟加拉玫瑰红(RB)制得一种高效的纳米光动力抗菌剂CS-RB,探究了其微观结构及光动力特性,并研究了其吸附织物后的光动力抗菌性能。结果表明:CS-RB生成^(1)O_(2)的能... 为解决光敏剂在蛋白质纤维应用上效能不足的问题,通过偶联壳聚糖(CS)和孟加拉玫瑰红(RB)制得一种高效的纳米光动力抗菌剂CS-RB,探究了其微观结构及光动力特性,并研究了其吸附织物后的光动力抗菌性能。结果表明:CS-RB生成^(1)O_(2)的能力提升2.98倍,对金黄葡萄球菌的抑菌率达99.99%,对大肠埃希菌的抑菌率提升了77.52个百分点。负载CS-RB的蛋白质纤维对金黄葡萄球菌和大肠埃希菌的抑菌率分别达到99.99%和99.90%,且具备优良的服用性能。此外,CS-RB对不同基材织物也显示出良好的光动力抗菌效能(99.99%) 展开更多
关键词 光动力抗菌 壳聚糖 孟加拉玫瑰红 纳米光敏剂 羊毛织物
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Wool/PTT纤维混纺制品的一浴染色技术研究 被引量:1
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作者 郑今欢 刘欣 +1 位作者 P.Rex Brady 王训该 《上海毛麻科技》 2010年第4期26-37,共12页
研究PTT短纤和羊毛混纺制品的染色技术以及混纺纤维的细度和拉伸性能。包括Terasil分散染料对PTT纤维织物的染色性能和对羊毛的沾色性;Lanasol活性染料对羊毛织物的染色性能和对PTT的沾色性;Terasil分散染料和毛用活性染料在同浴染色中... 研究PTT短纤和羊毛混纺制品的染色技术以及混纺纤维的细度和拉伸性能。包括Terasil分散染料对PTT纤维织物的染色性能和对羊毛的沾色性;Lanasol活性染料对羊毛织物的染色性能和对PTT的沾色性;Terasil分散染料和毛用活性染料在同浴染色中的相容性;pH值、温度、升温速度等工艺条件对PTT/羊毛混纺制品一浴染色中两种纤维同色性的影响。通过羊毛的碱溶率测试(按照澳大利亚标准)和FTIR图谱研究了不同染色温度和水处理温度下羊毛纤维的损伤程度。结果表明:细度相近的PTT短纤维与羊毛混纺可以提高羊毛制品的强度、抗变形性能而不失其柔韧性;PTT纤维属于易染型聚酯纤维,大部分Terasil分散染料在110℃左右即可达到其最大上染百分率;Terasil分散染料与Lanasol活性染料在小于120℃的弱酸浴中同浴染色具有很好的相容性;所研制的合适的染色工艺条件可以减轻Terasil分散染料对羊毛的沾色程度,使PTT和羊毛获得稳定的同色效果且耐洗色牢度优良,同时对羊毛纤维的损伤程度远低于常规的PET/羊毛混纺制品染色工艺。 展开更多
关键词 羊毛 PTT 一浴染色 相容性 沾色 羊毛的碱溶率
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Modal/Wool混纺精纺纱的实践 被引量:2
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作者 秦卫兵 《上海纺织科技》 北大核心 2004年第1期15-15,共1页
介绍新型绿色纤维Modal和毛混纺的生产工艺 ,对该生产工艺进行了研究与分析 。
关键词 MODAL纤维 毛纤维 混纺 精纺纱 生产工艺
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3D细胞培养技术及其在毛囊发育研究中的应用
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作者 孔辰 田云 +1 位作者 高红瑞 蔡蓓 《畜牧兽医学报》 北大核心 2025年第1期15-25,共11页
羊毛是重要的畜牧产品,由毛囊发育而来。毛囊是皮肤组织衍生的微器官,由毛囊干细胞、毛乳头、毛母质、外根鞘和内根鞘等20多种细胞组成,结构及细胞间通讯复杂。因此,提高羊毛产量和品质的前提是充分了解各种细胞相互作用下的毛囊发育特... 羊毛是重要的畜牧产品,由毛囊发育而来。毛囊是皮肤组织衍生的微器官,由毛囊干细胞、毛乳头、毛母质、外根鞘和内根鞘等20多种细胞组成,结构及细胞间通讯复杂。因此,提高羊毛产量和品质的前提是充分了解各种细胞相互作用下的毛囊发育特征。目前,细胞培养技术主要分为2D细胞培养和3D细胞培养。传统的2D细胞培养技术培养毛囊相关细胞时,与体内生长状况差距较大,毛发诱导能力逐渐丧失。3D细胞培养是将细胞在模拟组织和器官特异性的3D结构中进行培养,通过模拟天然环境,允许细胞聚集成为一定的形态,并且促进细胞-细胞的交流、细胞-细胞外基质的交流,从而恢复一定的在体功能,弥补了传统2D细胞培养的缺陷。本文简单介绍了3D细胞培养模型及种类、构建方法及在毛囊发育和生命科学相关研究中的应用,并展望了3D细胞培养技术在毛囊发育研究和其它领域的发展前景。 展开更多
关键词 3D细胞培养 毛囊 细胞外基质
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羊绒洗毛废水中羊绒脂的回收及其皂化工艺
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作者 王娅萱 徐鹏 +2 位作者 薛彦伦 梁栋明 项梅 《毛纺科技》 北大核心 2025年第1期61-67,共7页
为解决羊绒初加工工序中洗毛产生的废水污染问题,实现资源有效利用,通过对比实验采用絮凝、萃取工艺,得到粗制羊绒脂,以粗制羊绒脂为原材料利用冷、热制皂法制备羊绒脂皂。并以精制工艺、制皂溶液比例、温度、时间等为评价指标分析冷、... 为解决羊绒初加工工序中洗毛产生的废水污染问题,实现资源有效利用,通过对比实验采用絮凝、萃取工艺,得到粗制羊绒脂,以粗制羊绒脂为原材料利用冷、热制皂法制备羊绒脂皂。并以精制工艺、制皂溶液比例、温度、时间等为评价指标分析冷、热制皂情况。结果表明:絮凝工艺在洗毛废水pH值为8,投加2.5 g/L聚合硫酸铝铁絮凝剂进行絮凝,回收工艺以萃取法回收羊绒脂,羊绒脂回收率为73.8%;精制后的羊绒脂在热制皂工艺下皂化率为85.9%,综合评价发现热制皂性能整体优于冷制皂,热制羊绒脂皂的起泡能力为7.6 mL、泡沫稳定性1.6 mL、硬度6 mm、INS值165,可作为日化用品使用。 展开更多
关键词 洗毛废水 絮凝 制皂 资源回收
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Enzymatic Treatment of Wool 被引量:11
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作者 樊增禄 朱泉 戴瑾瑾 《Journal of Donghua University(English Edition)》 EI CAS 2001年第2期112-115,共4页
Three proteases were applied to treatments of wool. The shrinkage, dyeing behavior, some physical and mechanical properties of treated wool were studied. Peroxymonosulfate(PMS)and lipase were used to pretreat wool bef... Three proteases were applied to treatments of wool. The shrinkage, dyeing behavior, some physical and mechanical properties of treated wool were studied. Peroxymonosulfate(PMS)and lipase were used to pretreat wool before the treatment of it by protease. The results show that the shrink resistance of wool fabric is not obtained when it is treated only by the protease, but it is remarkably improved when the wool fabric is treated first by PMS or a lipase afterwards by protease The rate of dyeing is increased when the wool is treated by protease or combination of oxidant/lipase and protease. Whiteness, luster and softness of treated wool are improved, while the strength and elasticity are reduced. 展开更多
关键词 wool modification ENZYME
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Studies on Wool-Eating Ailment in Guizhou Semi-Fine Wool Sheep 被引量:5
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作者 SHEN Xiao-yun 《Agricultural Sciences in China》 CAS CSCD 2011年第10期1618-1623,共6页
The clinical symptoms of a disorder which is known locally as "wool-eating ailment" in Guizhou semi-fine wool sheep in the Weining County of Guizhou Province, China, are poor weight gain, lost appetite, pica, emacia... The clinical symptoms of a disorder which is known locally as "wool-eating ailment" in Guizhou semi-fine wool sheep in the Weining County of Guizhou Province, China, are poor weight gain, lost appetite, pica, emaciation, and wool-eating. The mineral composition of forage, and samples of blood, liver and wool from Guizhou semi-fine wool sheep in affected ranches were compared with those from the unaffected areas. The mean concentration of iron in forage from affected pasture was significantly higher than that from the unaffected pasture. The mean concentration of sulfur in forage from the affected and unaffected pasture was similar and within normal ranges. The mean concentration of sulfur in blood, liver and wool from the affected Guizhou semi-fine wool sheep was significantly lower than that from the unaffected sheep while the content of iron in blood, liver and wool from the affected sheep was significantly higher than that from the unaffected sheep. Oral administration of calcium sulfate prevented and cured the disease. We concluded that the disease of Guizhou semi-fine wool sheep in the Weining County is due to S deficiency caused by the high Fe in forages. 展开更多
关键词 Guizhou semi-fine wool sheep SULFUR IRON calcium sulfate wool-eating
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Status and development of mineral wool made from molten blast furnace slag 被引量:16
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作者 XIAO Yongli LIU Yin LI Yongqian 《Baosteel Technical Research》 CAS 2011年第2期3-8,共6页
This study describes the characteristics of mineral wool and its applications,and also introduces the traditional process of mineral wool made from molten blast furnace (BF) slag. Compared with high energy consumpti... This study describes the characteristics of mineral wool and its applications,and also introduces the traditional process of mineral wool made from molten blast furnace (BF) slag. Compared with high energy consumption of the traditional process,the production of mineral wool by using molten BF slag will be able to take full advantage of the sensible heat of molten slag, and also reduce production costs. However, there are also further issues to resolve such as how to obtain the required amount of molten BF slag and how to make it homogeneous. Based on the physical and chemical properties of the molten BF slag,the investigation into the relationship between temperature and viscosity under different acidity coefficients of the slag and silicon mixture was conducted. Combined with the crystallization and phase diagram of slag wool, its heat resistance, water resistance, durability and corrosion resistance were analyzed. Finally, trends of the technology development are proposed. 展开更多
关键词 mineral wool molten blast furnace (BF) slag fibering characteristics
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Effect of Testing Conditions on Fibre-Bundle Tensile Properties Part Ⅰ: Sample Preparation, Bundle Mass and Fibre Alignment of Wool Bundles 被引量:3
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作者 于伟东 严灏景 +1 位作者 Ron Postle Yang Shouren 《Journal of Donghua University(English Edition)》 EI CAS 2002年第2期74-80,共7页
Due to the effects of samples and testing conditions on fibre-bundle tensile behaviour, it is necessary to investigate the relationships between experimental factors and tensile properties for the fibre-bundle tensile... Due to the effects of samples and testing conditions on fibre-bundle tensile behaviour, it is necessary to investigate the relationships between experimental factors and tensile properties for the fibre-bundle tensile tester (TENSOR). The effects of bundle sample preparation, fibre bundle mass and fibre alignment have been tested. The experimental results indicated that (1) the low damage in combing and no free-end fibres in the cut bundle are most important for the sample preparation; (2) the reasonable bundle mass is 400700 tex, but the tensile properties measured should be modified with the bundle mass because a small amount of bundle mass causes the scatter results, while the larger is the bundle mass, the more difficult to comb fibres parallel and to clamp fibre evenly; and (3) the fibre irregular arrangement forms a slack bundle resulting in interaction between fibres, which will affect the reproducibility and accuracy of the tensile testing. 展开更多
关键词 FIBRE bundle wool tensile property modulus TENSOR
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Oil Content Conversion between Ether-based and Ethanol-based Methods and Establishment of Admissible Error for Imported Wool
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作者 罗丽疆 徐新龙 +6 位作者 刘东 哈森 刘佳丽 徐静 叶尔买克 戴莉 王海珍 《Agricultural Science & Technology》 CAS 2016年第1期23-25,33,共4页
Due to various reasons, the inspection methods often need to be changed, and the detection reagents often need to be replaced. In this study, a comparative experiment was conducted between the ethanol-based and ether-... Due to various reasons, the inspection methods often need to be changed, and the detection reagents often need to be replaced. In this study, a comparative experiment was conducted between the ethanol-based and ether-based determination methods for oil content in imported wool. The determination results obtained from the two methods were treated as abscissa and ordinate respectively,and their linear relationship was analyzed. According to the linear regression analysis, the conversion equation of determination result between the two methods was obtained. In addition, the repeatability admissible error and reproducibility admissible error were established through analyzing the comparative experimental results by scientific software. This study will bring new ideas for further researches in this field, and provide reference for solving the similar problems in actual inspection work. 展开更多
关键词 wool Oil content Determination method Exchange Admissible error
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Technology status and development of mineral wool made of blast furnace slag 被引量:4
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作者 XIAO Yongli,LI Yongqian and LIU Yin Research Institute,Baoshan Iron & Steel Co.,Ltd.,Shanghai 201900,China 《Baosteel Technical Research》 CAS 2010年第S1期137-,共1页
Under the trend of low-carbon economy,the technique of producing mineral wool insulation material from molten blast furnace slag are of great significance both to Insulation materials industry and metallurgical indust... Under the trend of low-carbon economy,the technique of producing mineral wool insulation material from molten blast furnace slag are of great significance both to Insulation materials industry and metallurgical industry on the aspects of energy conservation and emissions reduction.This paper presents characteristics and use of mineral wool made of blast furnace slag,and also introduces mineral wool production process and key techniques.It also put forward a number of issues need to be addressed in the process.The inherent mechanism affecting the performance of the of mineral wool is analyzed.And it points out the target and future direction of R & D in Baoshan Iron and Steel in mineral wool technology field. 展开更多
关键词 blast furnace slag mineral wool insulation material
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Identification of SNPs and expression patterns of FZD3 gene and its effect on wool traits in Chinese Merino sheep(Xinjiang Type) 被引量:3
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作者 ZHAO Bing-ru FU Xue-feng +9 位作者 TIAN Ke-chuan HUANG Xi-xia DI Jiang BAI Yan XU Xin-ming TIAN Yue-zhen WU Wei-wei ABLAT Sulayman ZENG Wei-dan HANIKEZI Tulafu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第10期2351-2360,共10页
As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits ... As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD)(P=0.04) and live weight (LW)(P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW)(P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P<0.05) than that of the fine wool (F) group. Moreover, it had a higher expression level (P<0.01) in skin tissues than in other tissues of Chinese Merino ewes. While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos. Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS). 展开更多
关键词 Chinese MERINO SHEEP (Xinjiang Type) FZD3 single NUCLEOTIDE polymorphism (SNP) expression pattern association analysis wool TRAITS
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