In vitro toxicologic study of chitin short fiber reinforced polycaprolactone composite

Objective: To investigate the cytotoxicity and cytocompatibility of chitin fiber reinforced polycaprolactone composite in vitro in order to provide useful scientific basis for clinical application. Methods: Cell morphology observation, MTT and DNA assay were used to evaluate the influence of the composite on the morphology, growth and proliferation of cultured L-929 cells. Results: The composite did not impair the morphology of cultured cells in vitro. MTT and DNA assay demonstrated that the growth and proliferation of the cultured cells were not significantly inhibited by the composite. Conclusion : The composites have fine cytocompatibility and are safe for clinical use of reconstruction of chest wall defects.

甲壳素短纤维增强聚己内酯复合材料的制备及生物学评价

用共混法制备甲壳素短纤维增强聚己内酯复合材料,并对纯聚己内酯和甲壳素短纤维增强聚己内酯复合材料进行体外细胞毒性和生物相容性评价,为临床应用提供有价值的实验依据。对该两种材料进行体外细胞毒性试验、急性全身毒性试验、溶血试验、热源试验和过敏试验。结果显示受试材料最终细胞毒性级为0级,对细胞生长和增殖无明显抑制作用,材料中不存在潜在致敏性物质,浸提液无溶血反应和急性全身毒性反应,无热源反应,表明该复合材料具有良好的细胞和组织相容性,其作为胸壁缺损修补材料应用于临床具有可行性和安全性。

甲壳素短纤维增强聚己内酯复合材料体外降解实验研究

将纯聚己内酯(PCL)、甲壳素短纤维增强PCL复合材料和聚DL-乳酸分别置于生理盐水溶液中,进行体外降解实验研究。于2、4、8、12、16、24周分别取材,测试降解液pH值、试样失重率及力学性能的变化,并行扫描电镜观察。结果显示甲壳素短纤维增强PCL初始强度大于纯PCL,在降解过程中,浸泡液pH值呈弱酸性和中性,失重速率快于纯PCL,力学性能先高后低,强度和模量值24周与初始值相差不大。通过以上体外降解实验可以得出结论:PCL树脂复合甲壳素纤维后,加快降解速度,提高力学性能,缓冲材料局部pH值的下降,是一种很有前途的胸壁缺损修补及骨科内固定材料。