AIM: To investigate the influence of chitosan nanofiber scaffold on the production and infectivity of porcine endogenous retrovirus (PERV) expressed by porcine hepatocytes. METHODS: Freshly isolated porcine hepatocyte...AIM: To investigate the influence of chitosan nanofiber scaffold on the production and infectivity of porcine endogenous retrovirus (PERV) expressed by porcine hepatocytes. METHODS: Freshly isolated porcine hepatocytes were cultured with or without chitosan nanofiber scaffold (defined as Nano group and Hep group) for 7 d. The daily collection of culture medium was used to detect reverse transcriptase (RT) activity with RT activity assaykits and PERV RNA by reverse transcription-polymerase chain reaction (PCR) and real time PCR with the PERV specific primers. And Western blotting was performed with the lysates of daily retrieved cells to determine the PERV protein gag p30. Besides, the in-vitro infectivity of the supernatant was tested by incubating the human embryo kidney 293 (HEK293) cells. RESULTS: The similar changing trends between two groups were observed in real time PCR, RT activity assay and Western blotting. Two peaks of PERV expression at 10H and Day 2 were found and followed by a regular decline. No significant difference was found between two groups except the significantly high level of PERV RNA at Day 6 and PERV protein at Day 5 in Nano group than that in Hep group. And in the in-vitro infection experiment, no HEK293 cell was infected by the supernatant. CONCLUSION: Chitosan nanofiber scaffold might prolong the PERV secreting time in pig hepatocytes but would not obviously influence its productive amount and infectivity, so it could be applied in the bioartificial liver without the increased risk of the virus transmission.展开更多
Currently,the gradual depletion of fossil resources and the large amount of plastic waste are causing serious harm to the land and marine ecology.The rapid development of wearable smart fibers is accompanied by rapid ...Currently,the gradual depletion of fossil resources and the large amount of plastic waste are causing serious harm to the land and marine ecology.The rapid development of wearable smart fibers is accompanied by rapid growth in the material demand for fibers,and the development of green and high-performance biomass-based fibers has become an important research topic to reduce the dependence on synthetic fiber materials and the harm to the environment.Here,chitosan is first prepared from the waste material by chemical methods.Then the chitosan-based self-powered induction fibers are prepared by electrospinning core wire technique.Chitosan-based self-powered sensing fiber is ultra-light and flexible,which can achieve about 2500 collisions without damaging the surface.Chitosan-based self-powered sensing fiber can also be used in smart home sensing applications to control home appliance switches with a light touch,which has a great application prospect in smart home and wearable fields.展开更多
基金Supported by The Natural Science Foundation of Jiangsu Province, No. BK2006008foundation of Medical Center of Jiangsu Province, No.ZX200605
文摘AIM: To investigate the influence of chitosan nanofiber scaffold on the production and infectivity of porcine endogenous retrovirus (PERV) expressed by porcine hepatocytes. METHODS: Freshly isolated porcine hepatocytes were cultured with or without chitosan nanofiber scaffold (defined as Nano group and Hep group) for 7 d. The daily collection of culture medium was used to detect reverse transcriptase (RT) activity with RT activity assaykits and PERV RNA by reverse transcription-polymerase chain reaction (PCR) and real time PCR with the PERV specific primers. And Western blotting was performed with the lysates of daily retrieved cells to determine the PERV protein gag p30. Besides, the in-vitro infectivity of the supernatant was tested by incubating the human embryo kidney 293 (HEK293) cells. RESULTS: The similar changing trends between two groups were observed in real time PCR, RT activity assay and Western blotting. Two peaks of PERV expression at 10H and Day 2 were found and followed by a regular decline. No significant difference was found between two groups except the significantly high level of PERV RNA at Day 6 and PERV protein at Day 5 in Nano group than that in Hep group. And in the in-vitro infection experiment, no HEK293 cell was infected by the supernatant. CONCLUSION: Chitosan nanofiber scaffold might prolong the PERV secreting time in pig hepatocytes but would not obviously influence its productive amount and infectivity, so it could be applied in the bioartificial liver without the increased risk of the virus transmission.
基金The authors are grateful for the support received from National Natural Science Foundation of China(Grant No.22109012)Natural Science Foundation of the Beijing Municipality(Grant No.2212052)the Fundamental Research Funds for the Central Universities(Grant No.E1E46805).
文摘Currently,the gradual depletion of fossil resources and the large amount of plastic waste are causing serious harm to the land and marine ecology.The rapid development of wearable smart fibers is accompanied by rapid growth in the material demand for fibers,and the development of green and high-performance biomass-based fibers has become an important research topic to reduce the dependence on synthetic fiber materials and the harm to the environment.Here,chitosan is first prepared from the waste material by chemical methods.Then the chitosan-based self-powered induction fibers are prepared by electrospinning core wire technique.Chitosan-based self-powered sensing fiber is ultra-light and flexible,which can achieve about 2500 collisions without damaging the surface.Chitosan-based self-powered sensing fiber can also be used in smart home sensing applications to control home appliance switches with a light touch,which has a great application prospect in smart home and wearable fields.
