A promising microalgal strain isolated from fresh water,which can grow both autotrophically on inorganic carbon under lighting and heterotrophically on organic carbon without lighting,was identified as Chlorella sp.US...A promising microalgal strain isolated from fresh water,which can grow both autotrophically on inorganic carbon under lighting and heterotrophically on organic carbon without lighting,was identified as Chlorella sp.USTB-01 with the phylogenetic analysis based on 18S ribosomal ribonucleic acid(rRNA)gene sequences.In the heterotrophic batch culture,more than 20.0 g·L^(-1)of cell dry weight concentration(DWC)of Chlorella sp.USTB-01 was obtained at day 5,and which was used directly to seed the autotrophic culture.A novel fermentor-helical combined photobioreactor was established and used to cultivate Chlorella sp.USTB-01 for the fixation of carbon dioxide(CO_(2)).It showed that the autotrophic growth of Chlorella sp.USTB-01 in the combined photobioreactor was more effective than that in the fermentor alone and the maximum DWC of 2.5 g·L^(-1)was obtained at day 6.The highest CO_(2)fixation of 95%appeared on day 1 in the exponential growth phases of Chlorella sp.USTB-01 and 49.8%protein was found in the harvested microalgal cells.展开更多
基金This research was supported by PetroChina Innovation Foundation(2009D-5006-04-02)the Fundamental Research Funds for the Central Universities and the Metallurgical Foundation of University of Science and Technology Beijing.
文摘A promising microalgal strain isolated from fresh water,which can grow both autotrophically on inorganic carbon under lighting and heterotrophically on organic carbon without lighting,was identified as Chlorella sp.USTB-01 with the phylogenetic analysis based on 18S ribosomal ribonucleic acid(rRNA)gene sequences.In the heterotrophic batch culture,more than 20.0 g·L^(-1)of cell dry weight concentration(DWC)of Chlorella sp.USTB-01 was obtained at day 5,and which was used directly to seed the autotrophic culture.A novel fermentor-helical combined photobioreactor was established and used to cultivate Chlorella sp.USTB-01 for the fixation of carbon dioxide(CO_(2)).It showed that the autotrophic growth of Chlorella sp.USTB-01 in the combined photobioreactor was more effective than that in the fermentor alone and the maximum DWC of 2.5 g·L^(-1)was obtained at day 6.The highest CO_(2)fixation of 95%appeared on day 1 in the exponential growth phases of Chlorella sp.USTB-01 and 49.8%protein was found in the harvested microalgal cells.
