We developed a technique of generating nonthermal atmospheric plasma-activated solution that had broad-spectrum antibacterial properties. Plasma-activated phosphate-buffered saline (PBS) causes rapid inactivation of b...We developed a technique of generating nonthermal atmospheric plasma-activated solution that had broad-spectrum antibacterial properties. Plasma-activated phosphate-buffered saline (PBS) causes rapid inactivation of bacteria following generation of oxidative stress. However, dose optimization requires understanding of cellular mechanisms. The objective of this study was to explore genome-wise response to develop gene expression profile of Escherichia coli using DNA microarray following exposure to plasma-activated PBS solution. Upon exposure to plasma-treated PBS solution, E. coli cells had differentially expressed genes involved in oxidative stress, and cell envelope and membrane associated porin and transporters. The genes involved in house-keeping and metabolism, energy generation, motility and virulence were conversely downregulated. This is the first report which demonstrates a severe oxidative stress induced in E. coli cells in response to an exposure to nonequilibrium nonthermal dielectric-barrier discharge plasma-activated PBS solution, and the genes that are responsive to reactive oxygen species appeared to play a role in cellular stress. Such studies are important to identify targets of inactivation, and to understand plasma-treated solution and bacterial cell interactions.展开更多
目的评价集成式微酸性次氯酸溶液对口腔综合治疗台水路系统(dental unit water lines,DUWLs)的持续消毒效果。方法以储水瓶定期人工配置含氯消毒液消毒DUWLs为对照组,以集成式微酸性次氯酸溶液(非电解,有效氯浓度为10 mg/L)持续消毒DUWL...目的评价集成式微酸性次氯酸溶液对口腔综合治疗台水路系统(dental unit water lines,DUWLs)的持续消毒效果。方法以储水瓶定期人工配置含氯消毒液消毒DUWLs为对照组,以集成式微酸性次氯酸溶液(非电解,有效氯浓度为10 mg/L)持续消毒DUWLs为实验组,采集口腔科4个功能区50台口腔综合治疗台的水源水、管路水(椅位前水)、椅位水(漱口、高速、三用枪)及末端水出水口水样,对比2种消毒方式细菌菌落计数。结果实验组的水路水(椅位前水)、椅位水的细菌微生物计数检测合格率为100.0%,高于对照组的60%,差异有统计学意义(P<0.05);实验组诊疗椅的高速水、三用枪水、漱口水的细菌微生物计数检测合格率(100.0%)高于对照组(43.8%),差异有统计学意义(P<0.05);结论口腔综合治疗台水路中存在微生物污染,使用微酸性次氯酸溶液(非电解,有效氯浓度为10 mg/L)持续消毒的有效性、高效性、稳定性和持续性效果明显,但对致病菌和生物膜的消毒效果还需进行更深入的研究。展开更多
文摘We developed a technique of generating nonthermal atmospheric plasma-activated solution that had broad-spectrum antibacterial properties. Plasma-activated phosphate-buffered saline (PBS) causes rapid inactivation of bacteria following generation of oxidative stress. However, dose optimization requires understanding of cellular mechanisms. The objective of this study was to explore genome-wise response to develop gene expression profile of Escherichia coli using DNA microarray following exposure to plasma-activated PBS solution. Upon exposure to plasma-treated PBS solution, E. coli cells had differentially expressed genes involved in oxidative stress, and cell envelope and membrane associated porin and transporters. The genes involved in house-keeping and metabolism, energy generation, motility and virulence were conversely downregulated. This is the first report which demonstrates a severe oxidative stress induced in E. coli cells in response to an exposure to nonequilibrium nonthermal dielectric-barrier discharge plasma-activated PBS solution, and the genes that are responsive to reactive oxygen species appeared to play a role in cellular stress. Such studies are important to identify targets of inactivation, and to understand plasma-treated solution and bacterial cell interactions.
文摘目的评价集成式微酸性次氯酸溶液对口腔综合治疗台水路系统(dental unit water lines,DUWLs)的持续消毒效果。方法以储水瓶定期人工配置含氯消毒液消毒DUWLs为对照组,以集成式微酸性次氯酸溶液(非电解,有效氯浓度为10 mg/L)持续消毒DUWLs为实验组,采集口腔科4个功能区50台口腔综合治疗台的水源水、管路水(椅位前水)、椅位水(漱口、高速、三用枪)及末端水出水口水样,对比2种消毒方式细菌菌落计数。结果实验组的水路水(椅位前水)、椅位水的细菌微生物计数检测合格率为100.0%,高于对照组的60%,差异有统计学意义(P<0.05);实验组诊疗椅的高速水、三用枪水、漱口水的细菌微生物计数检测合格率(100.0%)高于对照组(43.8%),差异有统计学意义(P<0.05);结论口腔综合治疗台水路中存在微生物污染,使用微酸性次氯酸溶液(非电解,有效氯浓度为10 mg/L)持续消毒的有效性、高效性、稳定性和持续性效果明显,但对致病菌和生物膜的消毒效果还需进行更深入的研究。
