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杨树叶绿体3′rps12基因,rps7基因和ndhB基因片段的克隆及序列分析 被引量:7
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作者 周奕华 侯丙凯 +4 位作者 石东乔 肖宇红 石锐 胡赞民 陈正华 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2001年第5期606-616,共11页
通过烟草叶绿体相关序列设计引物 ,从杨树的叶绿体基因组中克隆出 2个相邻的DNA片段 .经分析发现 ,这 2个DNA片段包含核糖体蛋白 3′rps12、rps7基因和NADH脱氢酶第二亚基ndhB基因片段 .利用DNAMAN等软件 ,将扩增到的杨树叶绿体DNA片段... 通过烟草叶绿体相关序列设计引物 ,从杨树的叶绿体基因组中克隆出 2个相邻的DNA片段 .经分析发现 ,这 2个DNA片段包含核糖体蛋白 3′rps12、rps7基因和NADH脱氢酶第二亚基ndhB基因片段 .利用DNAMAN等软件 ,将扩增到的杨树叶绿体DNA片段与烟草、拟南芥、玉米和黑松的相关序列进行比较 ,证实所扩增的片段具有较高的保守性 ,尤其是在这 3个基因的编码区 ,同源性均在 90 %以上 .插入或缺失常发生在基因间隔区 ,同源性在 80 %左右 ;对其编码区所推导的氨基酸序列进行了比较 ,同源性均在 92 %以上 .首次克隆了杨树叶绿体的部分DNA序列 ,详细报道并分析了杨树 3′rps12、rps7基因和ndhB基因片段及其边界序列信息 .所报告的基因序列均已登录GenBank . 展开更多
关键词 杨树 叶绿体 3′rps12基因 rps7基因 ndhB基因 基因克隆 序列分析
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静电场对植物光合器官结构和功能变化的影响 被引量:16
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作者 马福荣 许守民 +1 位作者 温尚斌 史晓琨 《生物物理学报》 CAS CSCD 北大核心 1994年第3期469-473,共5页
静电场促进水萝卜对矿质元素的吸收,叶片光合色素的含量及光合、呼吸活性增加,使叶片光合细胞的数目、细胞中叶绿体数目、叶绿体中基粒及类囊体片尾数都趋增加。结果表明,先合结构及活性的改善与静电场促进植物的离子吸收有关,从而... 静电场促进水萝卜对矿质元素的吸收,叶片光合色素的含量及光合、呼吸活性增加,使叶片光合细胞的数目、细胞中叶绿体数目、叶绿体中基粒及类囊体片尾数都趋增加。结果表明,先合结构及活性的改善与静电场促进植物的离子吸收有关,从而改善了植物的营养状况。 展开更多
关键词 静电场 矿质元素 光合活性 叶绿体 结构
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Chlorophyll Fluorescence as an Indicator of Cellular Damage by Glyphosate Herbicide in Raphanus sativus L.Plants 被引量:3
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作者 Fabia Barbosa Silva Alan Carlos Costa +1 位作者 Rodolfo Rodrigo Pereira Alves Clarice Aparecida Megguer 《American Journal of Plant Sciences》 2014年第16期2509-2519,共11页
The fodder radish is an important alternative late summer crop in central-western Brazil. The fodder radish has a short growing cycle, is highly productive and possesses qualities that enhance its cultivation potentia... The fodder radish is an important alternative late summer crop in central-western Brazil. The fodder radish has a short growing cycle, is highly productive and possesses qualities that enhance its cultivation potential as an alternative in Brazil’s Biodiesel Program. However, drift of herbicides, such as glyphosate, may hinder the development of fodder radishes. Studies of chlorophyll fluorescence can provide measurements that help assess the effect of herbicide, being good indicator of the damage to photosynthetic apparatus promoted by herbicide. This study was carried out to analyze the effects of glyphosate by measuring chlorophyll a fluorescence, cellular membrane stability and chloroplast pigment contents in fodder radish plants. This experiment used a randomized block design in which the treatments consisted of the following 6 different doses: control, 0, 150, 300, 450, 600, 750 and 900 g i.a. ha-1 of the recommended 480 g&middotL-1 dose of glyphosate acid equivalent. The plants were sprayed 30 days after emergence and were assessed every 48 hours. The characteristics evaluated, including chlorophyll a fluorescence, chloroplast pigments and the rate of electrolyte leakage, indicated that radish plants are sensitive to glyphosate because the treatments resulted in reduced levels of photosynthetic efficiency and increased damage to cellular membranes, which led to a reduction in the chloroplast pigment content. 展开更多
关键词 Pigments chloroplastidic EPSPS CHLOROPHYLL Fluorescence
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Green Fertilization Enhances the Photosynthetic Performance and the Growth of Leguminous Trees for Restoration Plantation in Central Amazon 被引量:1
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作者 Roberto Kirmayr Jaquetti Jose Francisco de Carvalho Goncalves +3 位作者 Joao Baptista Silva Ferraz Marciel J.Ferreira Ulysses Moreira dos Santos Junior Claudivan Feitosa de Lacerda 《American Journal of Plant Sciences》 2014年第16期2497-2508,共12页
The leguminous tree species Dipteryx odorata (Aubl.) Willd. and Inga edulis Mart. were grown in the field to reforest a degraded area. To investigate the photosynthetic responses and the initial growth of Amazonian fo... The leguminous tree species Dipteryx odorata (Aubl.) Willd. and Inga edulis Mart. were grown in the field to reforest a degraded area. To investigate the photosynthetic responses and the initial growth of Amazonian forest species under fertilization, the study species were subjected to different treatments: T1 = unfertilized-control, T2 = chemical fertilization, T3 = green fertilization and T4 = green and chemical fertilization. D. odorata showed the highest absolute growth rates in height under treatments T4 (10.2 cm&middotmonth-1) and T2 (12.2 cm&middotmonth-1). I. edulis exhibited the best performance for this same parameter in T4 (23.0 cm&middotmonth-1). The highest photosynthesis values (Pn) for both D. odorata (13.1 μmol&middotm-2&middots-1) and I. edulis (20.6 μmol&middotm-2&middots-1) were found in T4. The two species also showed the highest performance index values (PIABS) under T4 (D. odorata = 2.9 and I. edulis = 5.2). Our results suggest that the combination of green and chemical fertilization was the most effective of the four treatments evaluated for improving the photosynthetic performance and the growth of both species during the initial establishment of the species in a degraded area. 展开更多
关键词 PHOTOSYNTHESIS Chlorophyll a Fluorescence chloroplastid Pigment Contents Dipteryx odorata Inga edulis
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