It is urgent to find a technology accurately to better diagnose and treat to brain tumor.Eu-doped Gd2 O3 nanorods(Eu-Gd2 O3 NRs)with paramagnetic and fluorescent properties were conjugated with doxorubicin(Dox)and chl...It is urgent to find a technology accurately to better diagnose and treat to brain tumor.Eu-doped Gd2 O3 nanorods(Eu-Gd2 O3 NRs)with paramagnetic and fluorescent properties were conjugated with doxorubicin(Dox)and chlorotoxin(CTX)via PEGylation,hydrazone bond and sulfur bond(named as CTXNRs-Dox),and these NRs could release more Dox in lower pH environment.The results of cell experiments indicated that CTX-NRs-Dox had obvious targeting and toxic effects on U251 cells,as well as good fluorescence imaging behavior.The orthotopic glioma-transplanted mice models were constructed via the intracranial injection of glioma cells(U87 MG).The result of experiments after the tail-vein injection of the prepared NRs suggested that CTX-NRs-Dox could target to brain tumors via the long-time blood circulation,leading to their obvious contrast enhancement of MR imaging of the intracranial tumor and their significant inhibitory effect on the growth and metastasis of brain tumors.A mechanism of synergistic effect of CTX-NRs-Dox on targeting and inhabiting the brain tumor was proposed.Our research suggested that CTX-NRs-Dox had potential application prospect in the detection and treatment of glioma.展开更多
Precisely locating tumors always proves to be difficult.To find a molecule that can specifically bind to tumor cells is the key.Recently,chlorotoxin(CTX) has been proved to be able to bind to many kinds of tumor cells...Precisely locating tumors always proves to be difficult.To find a molecule that can specifically bind to tumor cells is the key.Recently,chlorotoxin(CTX) has been proved to be able to bind to many kinds of tumor cells.The CTX receptor on the cell surface has been demonstrated to be matrix metalloproteinase-2(MMP-2).Many researchers have combined CTX with other molecules,including 131I,Cy5.5,iron oxide nanoparticles coated by polyethylene glycol(NP-PEG),and so on,and thus synthesized various types of probes that can be detected by γ-camera,single photon emission computed tomography(SPECT) or magnetic resonance imaging(MRI).With these methods,the binding degree of CTX could be assessed.These studies demonstrated that CTX has a highly specific binding ability,high stability,and security.CTX could also inhibit or kill the tumor cells.A nonviral nanovector has been developed for gene therapy.As a result,it gradually develops into a new method of diagnosis and targeted therapy of tumors.This article reviews the current progress on CTX including the origin,chemical construction,the mechanism of binding with tumor cells,and the application to tumor imaging diagnosis and therapy.展开更多
基金supported by the National Natural Science Foundation of China (Nos.51273122,51872190)Sichuan Science and Technology Project (No.2018JY0535)supported by the Fundamental of Research Funds for the Central University (Nos.SCU2017A001,2018SCUH0024)
文摘It is urgent to find a technology accurately to better diagnose and treat to brain tumor.Eu-doped Gd2 O3 nanorods(Eu-Gd2 O3 NRs)with paramagnetic and fluorescent properties were conjugated with doxorubicin(Dox)and chlorotoxin(CTX)via PEGylation,hydrazone bond and sulfur bond(named as CTXNRs-Dox),and these NRs could release more Dox in lower pH environment.The results of cell experiments indicated that CTX-NRs-Dox had obvious targeting and toxic effects on U251 cells,as well as good fluorescence imaging behavior.The orthotopic glioma-transplanted mice models were constructed via the intracranial injection of glioma cells(U87 MG).The result of experiments after the tail-vein injection of the prepared NRs suggested that CTX-NRs-Dox could target to brain tumors via the long-time blood circulation,leading to their obvious contrast enhancement of MR imaging of the intracranial tumor and their significant inhibitory effect on the growth and metastasis of brain tumors.A mechanism of synergistic effect of CTX-NRs-Dox on targeting and inhabiting the brain tumor was proposed.Our research suggested that CTX-NRs-Dox had potential application prospect in the detection and treatment of glioma.
文摘Precisely locating tumors always proves to be difficult.To find a molecule that can specifically bind to tumor cells is the key.Recently,chlorotoxin(CTX) has been proved to be able to bind to many kinds of tumor cells.The CTX receptor on the cell surface has been demonstrated to be matrix metalloproteinase-2(MMP-2).Many researchers have combined CTX with other molecules,including 131I,Cy5.5,iron oxide nanoparticles coated by polyethylene glycol(NP-PEG),and so on,and thus synthesized various types of probes that can be detected by γ-camera,single photon emission computed tomography(SPECT) or magnetic resonance imaging(MRI).With these methods,the binding degree of CTX could be assessed.These studies demonstrated that CTX has a highly specific binding ability,high stability,and security.CTX could also inhibit or kill the tumor cells.A nonviral nanovector has been developed for gene therapy.As a result,it gradually develops into a new method of diagnosis and targeted therapy of tumors.This article reviews the current progress on CTX including the origin,chemical construction,the mechanism of binding with tumor cells,and the application to tumor imaging diagnosis and therapy.
