以质膜 Ca2 +通道阻断剂 L a Cl3和钙调素 (Ca M)拮抗剂氯丙嗪 (CPZ)对水稻幼苗预处理以阻碍 Ca2 +· Ca M信使传导后 ,研究了盐胁迫下 ,稻苗保护酶活性的变化。结果表明 :0 .15 mol/ L Na Cl胁迫下 ,L a Cl3和 CPZ预处理显著加剧...以质膜 Ca2 +通道阻断剂 L a Cl3和钙调素 (Ca M)拮抗剂氯丙嗪 (CPZ)对水稻幼苗预处理以阻碍 Ca2 +· Ca M信使传导后 ,研究了盐胁迫下 ,稻苗保护酶活性的变化。结果表明 :0 .15 mol/ L Na Cl胁迫下 ,L a Cl3和 CPZ预处理显著加剧稻苗过氧化氢酶 (CAT)活性和抗坏血酸过氧化物酶 (APX)活性下降 ;对谷胱甘肽还原酶 (GR)活性则无明显影响 ;而超氧化物岐化酶 (SOD)和过氧化物酶 (POD)活性前期无明显变化 ,仅在胁迫后期明显下降。盐胁迫下 ,L a Cl3和 CPZ预处理加剧 SOD、 CAT和 APX活性下降与加剧稻苗 MDA含量积累呈显著或极显著正相关。这暗示 Ca2 + · Ca展开更多
Hydrogen evolution reaction(HER),zinc corrosion,and dendrites growth on zinc metal anode are the major issues limiting the practical applications of zinc-ion batteries.Herein,an in-situ physical/chemical cross-linked ...Hydrogen evolution reaction(HER),zinc corrosion,and dendrites growth on zinc metal anode are the major issues limiting the practical applications of zinc-ion batteries.Herein,an in-situ physical/chemical cross-linked hydrogel electrolyte(carrageenan/polyacrylamide/ZnSO_(4),denoted as CPZ)has been developed to stabilize the zinc anode-electrolyte interface,which can eliminate side reactions and prevent dendrites growth.The in-situ CPZ hydrogel electrolyte improves the reversibility of zinc anode due to eliminating side reactions caused by active water molecules.Furthermore,the electrostatic interaction between the SO_(4)^(-)groups in CPZ and Zn^(2+)can encourage the preferential deposition of zinc atoms on(002)crystal plane,which achieve dendrite-free and homogeneous zinc deposition.The in-situ hydrogel electrolyte offers a streamlined approach to battery manufacturing by allowing for direct integration into the battery.Subsequently,the Zn//Zn half battery with CPZ hydrogel electrolyte can enable an ultra-long cycle over 5500 h at a current density of 0.5 mA cm^(-2),and the Zn//Cu half battery reach an average coulombic efficiency of 99.37%.The Zn//V_(2)O_5-GO full battery with CPZ hydrogel electrolyte demonstrates94.5%of capacity retention after 2100 cycles.This study is expected to open new thought for the development of commercial hydrogel electrolytes for low-cost and long-life zinc-ion batteries.展开更多
A simple, rapid and sensitive method termed as magnetic solid phase extraction （MSPE） combined with high-performance liquid chromatography-ultraviolet detector （HPLC-UV） has been proposed for the determination of ...A simple, rapid and sensitive method termed as magnetic solid phase extraction （MSPE） combined with high-performance liquid chromatography-ultraviolet detector （HPLC-UV） has been proposed for the determination of trace amounts of chlorpromazine （CPZ） in water, urine and plasma samples. The separation and determination was performed on a C18 column under the optimal chromatographic conditions. Several factors influencing the extraction efficiency of CPZ, such as pH, surfactant and adsorbent amounts, ionic strength, extraction time, sample volume and desorption conditions, were studied and optimized. Under the optimal MSPE conditions, the extraction percentage of CPZ was 74%, 27% and 16% in water, urine and plasma samples, respectively. The limits of detection （LODs） of the proposed approach were 0.1, 5.0 and 10ng/mL in water, urine and plasma samples, respectively. The relative standard deviations （RSDs） based on five replicate determinations at 10 ng/mL level of CPZ was 1.2%. Good linear behaviors over the investigated concentration ranges （0.25-300 ng/mL） with good coefficient of determination, R2 〉 0.9998, were obtained. Good spike recoveries with relative errors less than 9.0% were obtained when applying the proposed method to water, urine and plasma samples.展开更多
It was shown that a new sensitive spectrophotometric method for the determination oftrace arsenic with chlorpromazine by the formation of heteropoly arsenomolybdic chlorpromazinecomplex in aqueous phase.
A simple method has been proposed for the determination of clozapine （CLZ） and chlorpromazine （CPZ） in human urine by dispersive liquid-liquid microextraction （DLLME） in combination with high-performance liquid ...A simple method has been proposed for the determination of clozapine （CLZ） and chlorpromazine （CPZ） in human urine by dispersive liquid-liquid microextraction （DLLME） in combination with high-performance liquid chromatography-ultraviolet detector （HPLC-UV）. All important variables influencing the extraction efficiency, such as pH, types of the extraction solvent and the disperser solvent and their volume, ionic strength and centrifugation time were investigated and optimized. Under the optimal conditions, the limit of detection （LODs） and quantification （LOQs） of the method were 13 and 39 ng/mL for CLZ, and 2 and 6 ng/mL for CPZ, respectively. The relative standard deviations （RSDs） of the targets were less than 5.1% （C=0.100 μg/mL, n=9）. Good linear behaviors over the tested concentration ranges were obtained with the values of R20.999 for the targets. The absolute extraction efficiencies of CLZ and CPZ from the spiked blank urine samples were 98.3% and 97.8%, respectively. The applicability of the technique was validated by analyzing urine samples and the mean recoveries for spiked urine samples ranged from 93.3% to 105.0%. The method was successfully applied for the determination of CLZ and CPZ in real human urine.展开更多
Cadmium (Cd), one of the most dangerous heavy metals, has a very similar ionic radius to calcium (Ca). The interference of cadmium in calcium homeostasis may play an important role in cadmium toxicity. Recent reports ...Cadmium (Cd), one of the most dangerous heavy metals, has a very similar ionic radius to calcium (Ca). The interference of cadmium in calcium homeostasis may play an important role in cadmium toxicity. Recent reports indicate that calmodulin (CaM) inhibitors such as trifluoperazine and chlorpromazine (CPZ) could protect rodents against cadmium toxicity. It was also reported that pretreatment of mice with zinc (Zn) could reduce the adverse effects induced by cadmium. The aim of this study is to determine whether Cd changes the balance of other essential metals such as Zn and copper (Cu) in rat tissues, and whether CPZ can reverse these changes which are induced by cadmium intoxication. Adult male Sprague Dawley (SD) rats were injected intraperitoneally (ip) with cadmium chloride (CdCl 2) (0.2, 0.4, 0.8 mg Cd/kg body weight) alone and 0.4mg Cd/kg in association with CPZ (5 mg/kg) daily for a week. The control animals were injected with normal saline only. The results showed that the cadmium content in the liver, kidney and testis increased significantly with a dose response relationship. Cadmium treatment markedly increased the Zn and Ca content in some of the tissues. Hepatic and renal metallothionein (MT) increased significantly after cadmium intoxication. CPZ treatment, however, reduced cadmium content in liver, but not blood and kidney. CPZ seemed to decrease the content of MT in liver and significantly increase the amounts of MT in kidney. These data suggest that the intervention of cadmium with tissue essential metals may play a role in cadmium toxicity in rats, and calmodulin inhibitors to some extent can reduce the adverse effect of cadmium by decreasing the cadmium load in tissues and reversing the unbalance of essential metals.展开更多
文摘以质膜 Ca2 +通道阻断剂 L a Cl3和钙调素 (Ca M)拮抗剂氯丙嗪 (CPZ)对水稻幼苗预处理以阻碍 Ca2 +· Ca M信使传导后 ,研究了盐胁迫下 ,稻苗保护酶活性的变化。结果表明 :0 .15 mol/ L Na Cl胁迫下 ,L a Cl3和 CPZ预处理显著加剧稻苗过氧化氢酶 (CAT)活性和抗坏血酸过氧化物酶 (APX)活性下降 ;对谷胱甘肽还原酶 (GR)活性则无明显影响 ;而超氧化物岐化酶 (SOD)和过氧化物酶 (POD)活性前期无明显变化 ,仅在胁迫后期明显下降。盐胁迫下 ,L a Cl3和 CPZ预处理加剧 SOD、 CAT和 APX活性下降与加剧稻苗 MDA含量积累呈显著或极显著正相关。这暗示 Ca2 + · Ca
基金supported by the Key Program of Natural Science Foundation of Gansu Province (23JRRA789)the Major Science and Technology Project of Gansu Province (22ZD6GA008)。
文摘Hydrogen evolution reaction(HER),zinc corrosion,and dendrites growth on zinc metal anode are the major issues limiting the practical applications of zinc-ion batteries.Herein,an in-situ physical/chemical cross-linked hydrogel electrolyte(carrageenan/polyacrylamide/ZnSO_(4),denoted as CPZ)has been developed to stabilize the zinc anode-electrolyte interface,which can eliminate side reactions and prevent dendrites growth.The in-situ CPZ hydrogel electrolyte improves the reversibility of zinc anode due to eliminating side reactions caused by active water molecules.Furthermore,the electrostatic interaction between the SO_(4)^(-)groups in CPZ and Zn^(2+)can encourage the preferential deposition of zinc atoms on(002)crystal plane,which achieve dendrite-free and homogeneous zinc deposition.The in-situ hydrogel electrolyte offers a streamlined approach to battery manufacturing by allowing for direct integration into the battery.Subsequently,the Zn//Zn half battery with CPZ hydrogel electrolyte can enable an ultra-long cycle over 5500 h at a current density of 0.5 mA cm^(-2),and the Zn//Cu half battery reach an average coulombic efficiency of 99.37%.The Zn//V_(2)O_5-GO full battery with CPZ hydrogel electrolyte demonstrates94.5%of capacity retention after 2100 cycles.This study is expected to open new thought for the development of commercial hydrogel electrolytes for low-cost and long-life zinc-ion batteries.
文摘A simple, rapid and sensitive method termed as magnetic solid phase extraction （MSPE） combined with high-performance liquid chromatography-ultraviolet detector （HPLC-UV） has been proposed for the determination of trace amounts of chlorpromazine （CPZ） in water, urine and plasma samples. The separation and determination was performed on a C18 column under the optimal chromatographic conditions. Several factors influencing the extraction efficiency of CPZ, such as pH, surfactant and adsorbent amounts, ionic strength, extraction time, sample volume and desorption conditions, were studied and optimized. Under the optimal MSPE conditions, the extraction percentage of CPZ was 74%, 27% and 16% in water, urine and plasma samples, respectively. The limits of detection （LODs） of the proposed approach were 0.1, 5.0 and 10ng/mL in water, urine and plasma samples, respectively. The relative standard deviations （RSDs） based on five replicate determinations at 10 ng/mL level of CPZ was 1.2%. Good linear behaviors over the investigated concentration ranges （0.25-300 ng/mL） with good coefficient of determination, R2 〉 0.9998, were obtained. Good spike recoveries with relative errors less than 9.0% were obtained when applying the proposed method to water, urine and plasma samples.
文摘It was shown that a new sensitive spectrophotometric method for the determination oftrace arsenic with chlorpromazine by the formation of heteropoly arsenomolybdic chlorpromazinecomplex in aqueous phase.
基金supported by Science Research Funds of Medical Course, HUST
文摘A simple method has been proposed for the determination of clozapine （CLZ） and chlorpromazine （CPZ） in human urine by dispersive liquid-liquid microextraction （DLLME） in combination with high-performance liquid chromatography-ultraviolet detector （HPLC-UV）. All important variables influencing the extraction efficiency, such as pH, types of the extraction solvent and the disperser solvent and their volume, ionic strength and centrifugation time were investigated and optimized. Under the optimal conditions, the limit of detection （LODs） and quantification （LOQs） of the method were 13 and 39 ng/mL for CLZ, and 2 and 6 ng/mL for CPZ, respectively. The relative standard deviations （RSDs） of the targets were less than 5.1% （C=0.100 μg/mL, n=9）. Good linear behaviors over the tested concentration ranges were obtained with the values of R20.999 for the targets. The absolute extraction efficiencies of CLZ and CPZ from the spiked blank urine samples were 98.3% and 97.8%, respectively. The applicability of the technique was validated by analyzing urine samples and the mean recoveries for spiked urine samples ranged from 93.3% to 105.0%. The method was successfully applied for the determination of CLZ and CPZ in real human urine.
基金ThisprojectwasgrantedbyNationalNaturalScienceFoundationofChina (No :396 4 0 0 0 6 )
文摘Cadmium (Cd), one of the most dangerous heavy metals, has a very similar ionic radius to calcium (Ca). The interference of cadmium in calcium homeostasis may play an important role in cadmium toxicity. Recent reports indicate that calmodulin (CaM) inhibitors such as trifluoperazine and chlorpromazine (CPZ) could protect rodents against cadmium toxicity. It was also reported that pretreatment of mice with zinc (Zn) could reduce the adverse effects induced by cadmium. The aim of this study is to determine whether Cd changes the balance of other essential metals such as Zn and copper (Cu) in rat tissues, and whether CPZ can reverse these changes which are induced by cadmium intoxication. Adult male Sprague Dawley (SD) rats were injected intraperitoneally (ip) with cadmium chloride (CdCl 2) (0.2, 0.4, 0.8 mg Cd/kg body weight) alone and 0.4mg Cd/kg in association with CPZ (5 mg/kg) daily for a week. The control animals were injected with normal saline only. The results showed that the cadmium content in the liver, kidney and testis increased significantly with a dose response relationship. Cadmium treatment markedly increased the Zn and Ca content in some of the tissues. Hepatic and renal metallothionein (MT) increased significantly after cadmium intoxication. CPZ treatment, however, reduced cadmium content in liver, but not blood and kidney. CPZ seemed to decrease the content of MT in liver and significantly increase the amounts of MT in kidney. These data suggest that the intervention of cadmium with tissue essential metals may play a role in cadmium toxicity in rats, and calmodulin inhibitors to some extent can reduce the adverse effect of cadmium by decreasing the cadmium load in tissues and reversing the unbalance of essential metals.
