A novel choline oxidase electrode was constructed by entrapping choline oxidase into polyaniline polyacrylonitrile composite film. The enzyme film was prepared by in situ electropolyme rization of aniline into p...A novel choline oxidase electrode was constructed by entrapping choline oxidase into polyaniline polyacrylonitrile composite film. The enzyme film was prepared by in situ electropolyme rization of aniline into porous polyacrylonitrile coated platinum electrode in the presence of choline oxidase. The enzyme electrode exhibited sensitive and stable electrochemical response to choline. The kinetics analysis showed that the mass transport is partially rate limiting. The influences of pH, applied potential and temperature on the response of the enzyme electrode were also described.展开更多
文摘A novel choline oxidase electrode was constructed by entrapping choline oxidase into polyaniline polyacrylonitrile composite film. The enzyme film was prepared by in situ electropolyme rization of aniline into porous polyacrylonitrile coated platinum electrode in the presence of choline oxidase. The enzyme electrode exhibited sensitive and stable electrochemical response to choline. The kinetics analysis showed that the mass transport is partially rate limiting. The influences of pH, applied potential and temperature on the response of the enzyme electrode were also described.
