Drosophila dEAAT2, a member of the excitatory amino-acid transporter(EAAT) family, has been described as mediating the high-affinity transport of taurine, which is a free amino-acid abundant in both insects and mammal...Drosophila dEAAT2, a member of the excitatory amino-acid transporter(EAAT) family, has been described as mediating the high-affinity transport of taurine, which is a free amino-acid abundant in both insects and mammals. However, the role of taurine and its transporter in hearing is not clear. Here, we report that dEAAT2 is required for the larval startle response to sound stimuli. d EAAT2 was found to be enriched in the distal region of chordotonal neurons where sound transduction occurs. The Ca2+imaging and electrophysiological results showed that disrupted dEAAT2 expression significantly reduced the response of chordotonal neurons to sound.More importantly, expressing d EAAT2 in the chordotonal neurons rescued these mutant phenotypes. Taken together,these findings indicate a critical role for Drosophila dEAAT2 in sound transduction by chordotonal neurons.展开更多
Chordotonal neurons are responsible for sound sensation in Drosophila.However,little is known about how they respond to sound with high sensitivity.Using genetic labeling,we found one of the Drosophila axonemal dynein...Chordotonal neurons are responsible for sound sensation in Drosophila.However,little is known about how they respond to sound with high sensitivity.Using genetic labeling,we found one of the Drosophila axonemal dynein heavy chains,CG9492(DNAH5),was specifically expressed in larval chordotonal neurons and showed a distribution restricted to proximal cilia.While DNAH5 mutation did not affect the cilium morphology or the trafficking of Inactive,a candidate auditory transduction channel,larvae with DNAH5 mutation had reduced startle responses to sound at low and medium intensities.Calcium imaging confirmed that DNAH5 functioned autonomously in chordotonal neurons for larval sound sensation.Furthermore,disrupting DNAH5 resulted in a decrease of spike firing responses to low-level sound in chordotonal neurons.Intriguingly,DNAH5 mutant larvae displayed an altered frequency tuning curve of the auditory organs.All together,our findings support a critical role of DNAH5 in tuning the frequency selectivity and the sound sensitivity of larval auditory neurons.展开更多
基金supported by funds from The Ministry of Science and Technology of China (2017YFA0103900 and 2016YFA0502800)The National Natural Science Foundation of China (31571083)+6 种基金supported by The National Natural Science Foundation of China (81470701)The National Natural Science Foundation of China (81771882)The Program for Professor of Special Appointment (Eastern Scholar of Shanghai TP2014008)The Shanghai Rising-Star Program (14QA1400800)a grant from the Young 1000 Talent Program of China to ZYThe Fundamental Research (Discipline Layout) Foundation from Shenzhen Committee of Science, Technology and Innovation (JCYJ20170817111912585) to FC
文摘Drosophila dEAAT2, a member of the excitatory amino-acid transporter(EAAT) family, has been described as mediating the high-affinity transport of taurine, which is a free amino-acid abundant in both insects and mammals. However, the role of taurine and its transporter in hearing is not clear. Here, we report that dEAAT2 is required for the larval startle response to sound stimuli. d EAAT2 was found to be enriched in the distal region of chordotonal neurons where sound transduction occurs. The Ca2+imaging and electrophysiological results showed that disrupted dEAAT2 expression significantly reduced the response of chordotonal neurons to sound.More importantly, expressing d EAAT2 in the chordotonal neurons rescued these mutant phenotypes. Taken together,these findings indicate a critical role for Drosophila dEAAT2 in sound transduction by chordotonal neurons.
基金the National Key R&D Program of China Project(2017YFA0103900 and 2016YFA0502800)the National Natural Science Foundation of China(31571083 and 31970931)+2 种基金the Program for Professor of Special Appointment(Eastern Scholar of Shanghai,TP2014008)the Shanghai Municipal Science and Technology Major Project(2017SHZDZX01 and 2018SHZDZX01)ZJLab,and the Shanghai Rising-Star Program(14QA1400800)。
文摘Chordotonal neurons are responsible for sound sensation in Drosophila.However,little is known about how they respond to sound with high sensitivity.Using genetic labeling,we found one of the Drosophila axonemal dynein heavy chains,CG9492(DNAH5),was specifically expressed in larval chordotonal neurons and showed a distribution restricted to proximal cilia.While DNAH5 mutation did not affect the cilium morphology or the trafficking of Inactive,a candidate auditory transduction channel,larvae with DNAH5 mutation had reduced startle responses to sound at low and medium intensities.Calcium imaging confirmed that DNAH5 functioned autonomously in chordotonal neurons for larval sound sensation.Furthermore,disrupting DNAH5 resulted in a decrease of spike firing responses to low-level sound in chordotonal neurons.Intriguingly,DNAH5 mutant larvae displayed an altered frequency tuning curve of the auditory organs.All together,our findings support a critical role of DNAH5 in tuning the frequency selectivity and the sound sensitivity of larval auditory neurons.
