Effects of Calcium Signal on the Negative Phototropism of Chorophytum comosum var.variegata Root

[Objective] The aim was to explore the effects of Ca2＋ on the negative phototropism of Chorophytum comosum root.[Method] C.comosum were cultivated in different concentration of CaCl2 solution with unilateral light illuminated.[Rusult] The Ca2＋ in nutrient solution showed significant effect on the growth and tropic reaction of C.comosum;low concentration of Ca2＋ promoted root growth,while high concentrations of Ca2＋ inhibited the root growth.When the concentration of Ca2＋ was 0.4 mmol/L,the phenomenon of the negative phototropism and the root growth were the most obvious.[Conclusion] The root negative phototropism could be concluded to the comprehensive effect of the endogenous and exogenous Ca2＋.

白纹草的组织培养和快速繁殖技术研究

为提高白纹草(Chorophytum bichetii)种苗质量和繁殖效率,以其顶芽或茎段为外植体材料,比较不同实验时间外植体的灭菌效果,筛选诱导丛生芽和继代繁殖的最佳激素配比,建立白纹草离体快繁的技术体系。结果表明,3月取材,外植体的灭菌效果最好,顶芽和嫩茎段的灭菌率分别为74.7%和82.7%;以MS为基本培养基,附加6-BA 2 mg/L+IBA 0.2 mg/L为最佳诱导丛生芽的培养基;以6-BA 2 mg/L+KT 0.2 mg/L为最佳继代增殖培养基,增殖系数达5.1;使用较高浓度6-BA时会发生较严重的玻璃化现象。以1/2MS+NAA 0.1 mg/L为最佳生根培养基,生根率100%;根长0.5 cm左右时最适宜移栽。用泥炭和珍珠岩按2:1混合的基质移栽,移栽成活率达99%以上,并且种苗健壮。在保证繁殖系数前提下尽量降低细胞分裂素浓度,并在继代中不断进行筛选剔除变异株。该技术体系能够满足规模化生产的要求。