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Chromosome G-banding in SituHybridization of RFLP Marker umc58 Linked with the Gene hm1 Dictating Helminthosporium carbonum Susceptibility1 in Maize 被引量:2
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作者 Song Yunchun Ren Nan +1 位作者 Mao Ninghui Liu Lihua 《Wuhan University Journal of Natural Sciences》 CAS 1997年第2期99-103,共5页
The technique of simultaneous G banding and in situ hybridization has been developed in plants for the first time.Using this technique.RFLP marker umc58 closely linked with the hm1 gene dictating Helminthosporium carb... The technique of simultaneous G banding and in situ hybridization has been developed in plants for the first time.Using this technique.RFLP marker umc58 closely linked with the hm1 gene dictating Helminthosporium carbonum susceptibility1 was localized onto 1L3(chromosome 1,long arm,the third band from the centromere to the end of the arm),5L5 and 9L5.Theresults demonstrated that umc58 was a tripli cated sequence.It was deduced that umc58 probably was in a duplicated region that includes a part of Helminthosporium carbonum susceptibility genes(hm1 and hm2),as the hybridization sites of umc58 in chro mosomes 1 and 9 were those at which the genes localize.The techniques of simultaneous G banding and ISH in plants are discussed. 展开更多
关键词 In situ hybridization chromosome G banding MAIZE RFLP markers
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药用植物丹参的核型分析和C-banding研究 被引量:4
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作者 唐晓清 吴健 +2 位作者 余伯阳 王康才 别同德 《江西农业学报》 CAS 2006年第3期75-77,85,共4页
采用传统的核型分析技术,分析了栽培丹参有丝分裂中期染色体的核型和C—banding。结果表明:丹参中期染色体长度为1.30-2.39μm,核型公式是2n=16=4m+2sm+2st,其核型为2A型;单套染色体组显示出19条清晰稳定的C—bands,其中包括1... 采用传统的核型分析技术,分析了栽培丹参有丝分裂中期染色体的核型和C—banding。结果表明:丹参中期染色体长度为1.30-2.39μm,核型公式是2n=16=4m+2sm+2st,其核型为2A型;单套染色体组显示出19条清晰稳定的C—bands,其中包括13条末端带,1条着丝粒带和5条中间带。 展开更多
关键词 丹参 染色体 核型 C—banding
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Using the Maximum Effective Moment Criterion to Interpret Quartz -Fabric Patterns 被引量:1
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作者 ZHENG Yadong ZHANG Jinjiang ZHANG Bo 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2019年第4期799-809,共11页
The Maximum Effective Moment(MEM)criterion predicts that the initial orientation of ductile shear zones and shear bands is^55°relative to the maximum principal stress axis(σ1)and that the kinematic vorticity num... The Maximum Effective Moment(MEM)criterion predicts that the initial orientation of ductile shear zones and shear bands is^55°relative to the maximum principal stress axis(σ1)and that the kinematic vorticity number(Wk)is^0.94.These preferred orientations should be reflected in the pattern of quartz<c>-fabrics in shear zones and shear bands.Common quartz<c>-fabrics in plane strain can be divided into low-temperature(L)and high-temperature(H)fabrics,with each group showing three patterns.A steady flow with a constant value of Wk≈0.94 gives rise to L-1 and H-1 patterns,which are commonly characterized by a single<c>axis girdle normal to the shear zone and a single<c>-point maximum parallel to the shear zone.Once the conjugate set develops,L-1 and H-1 have opening angles of^70°and^110°,respectively.L-2 and H-2 are asymmetric patterns associated with variable deformation partitioning and vorticity values of0<Wk<0.94.In contrast,L-3 and H-3 are symmetric patterns associated with 100%deformation partitioning and Wk=0.The opening angle in quartz<c>-fabrics is implicitly linked to the temperature during deformation.The opening angle is^70°at low temperature and^110°at high temperature.However,a linear correction between the opening angle and the temperature cannot be established.During deformation partitioning,synthetic shear bands form earlier than antithetic bands and are more easily developed.This may result in opening angles of<70°for low-temperature fabrics and of>110°for high-temperature fabrics.The following criteria can be used to recognize reworked shear zones that have experienced multiple orogenic phases and changes in the stress state:1)the initial Wk is larger or smaller than^0.94;2)the change in Wk is abrupt,rather than progressive;3)inconsistent shear senses are inferred for the different phases of deformation;and4)a negative value of Wk is found in reworked shear zones. 展开更多
关键词 deformation localization deformation partitioning shear bands vorticity Wk quartz CPO patterns
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Structural Changes of 2V Chromosome of Haynaldia villosa Induced by Gametocidal Chromosome 3C of Aegilops triuncialis 被引量:1
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作者 CHEN Quan-zhan CAO Ai-zhong +2 位作者 QI Zeng-jun ZHANG Wei CHEN Pei-du 《Agricultural Sciences in China》 CAS CSCD 2008年第7期804-811,共8页
Haynaldia villosa (2n=2X= 14, VV), a relative of wheat, plays important roles in wheat improvement mainly owing to its disease resistance. Powdery mildew resistance gene Pm21 has been successfully transferred into w... Haynaldia villosa (2n=2X= 14, VV), a relative of wheat, plays important roles in wheat improvement mainly owing to its disease resistance. Powdery mildew resistance gene Pm21 has been successfully transferred into wheat by Cytogenetic Institute, Nanjing Agricultural University, China, and is widely used in the current wheat breeding programs. In this research, our objective is to further transfer and utilize the beneficial genes such as eye-spot resistance, yellow rust resistance, and gene of the tufted bristles on the glume ridge (a remarkable morphology) mapped on 2V of Haynaldia villosa. A disomic addition line with gametocidal chromosome 3C ofAegilops triuncialis added in Norin-26 was crossed to the wheat-H, villosa disomic substitution 2V(2D) and the hybrid F1 was then self-crossed. Chromosome C-banding, genomic in situ hybridization (GISH), and meiotic analysis in combination with molecular markers were applied to detect the chromosome variations derived from hybrids Fz and F3. To date, four translocations including one small segmental translocation T6BS·6BL-2VS, two whole arm translocations (preliminarily designed as T3DS·2VL and T2VS.7DL) and one intercalary translocation T2VS·2VL-W-2VL, one deletion Del. 2VS·2VL-, one monotelosomic Mt2VS, and one isochromosome 2VS·2VS line have been developed and characterized. One wheat SSR marker Xwmc25.120 tagging 2VS and one wheat STS marker NAU/STSBCD135-1 (2BL) tagging 2VL were successfully used to confirm the alien chromosome segments involved in the seven lines. The tufted bristles on the glume ridge appeared in lines T2VS-7DL, Mt2VS, 2VS-2VS as well as the parent DS2V(2D), whereas in T3DS·2VL, this trait did not appear. The gene controlling the tufted bristles was located on 2VS. Gametocidal chromosome 3C ofAegilops triuncialis could successfully induce chromosome 2V structural changes. 展开更多
关键词 Triticum aestivum Haynaldia villosa gametocidal chromosome 3C chromosome aberration C-banding genomic in situ hybridization molecular marker
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Genomic insights into hybridization in a localized region of sympatry between pewee sister species(Contopus sordidulus × C. virens) and their chromosomal patterns of differentiation
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作者 Joseph D.Manthey Mark B.Robbins 《Chinese Birds》 CSCD 2016年第1期48-55,共8页
Background: The Great Plains of the United States includes a large number of hybrid and contact zones between bird species. The amount of gene flow between sister species in these zones ranges from very rare hybridiza... Background: The Great Plains of the United States includes a large number of hybrid and contact zones between bird species. The amount of gene flow between sister species in these zones ranges from very rare hybridization events to widespread and prevalent introgression. Some of these avian systems have been studied extensively, while others have been indeterminate of whether hybridization exists in areas of sympatry. Using genomic-level approaches allows investigation of genomic patterns of hybridization and gene flow between species—or lack thereof.Methods: We investigated a narrow zone of sympatry in Nebraska, USA between pewee species(Contopus sordidulus and C. virens), for which no hybridization has been confirmed. We used thousands of single nucleotide polymorphisms to identify potential hybridization and investigate genomic patterns of differentiation between these two species.Results: We found evidence of multiple hybrid individuals in the contact zone. Little genomic variation was fixed between species, but a large proportion had differentiated allele frequencies between species. There was a positive relationship between genetic differentiation and chromosome size.Conclusions: We provided the first conclusive evidence of hybridization between C. sordidulus and C. virens, in a region where secondary contact likely occurred due to human disturbance and habitat modification. The genomic patterns of differentiation affirm that these species split in the relatively recent past. Finally, the relationship of chromosome size and genetic differentiation may have resulted from differential rates of chromosomal recombination in songbirds and genetic differentiation between species largely due to genetic drift(possibly in concert with selection). 展开更多
关键词 Contact ZONE Hybrid ZONE HYBRIDIZATION CHROMOSOMAL patterns Next-generation sequencing Contopus North AMERICA
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Molecular Profile of Human Serine Palmitoyltransferase-1 Proximate of Chromosome 9 Disease Susceptibility Gene Cluster in Inflammatory Cancer Cell Lines
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作者 Tokunbo Yerokun Tamra Neblett Chénel Johnson 《Journal of Cancer Therapy》 2014年第10期885-901,共17页
Background: Over 1100 genes have been annotated for human chromosome 9, including disease genes implicated in inflammation, atherosclerosis, cancer and neurodegeneration. The serine palmitoyltransferase-1, SPTLC1, gen... Background: Over 1100 genes have been annotated for human chromosome 9, including disease genes implicated in inflammation, atherosclerosis, cancer and neurodegeneration. The serine palmitoyltransferase-1, SPTLC1, gene is at the 9q22.2 cytogenetic band, a high G+C content region with common genetic alterations sufficient to modify cellular behavior. The sequence is highly conserved among diverse species from bacteria to humans, including a recently discovered 126 nucleotide alternate open reading frame, AltORF. The protein encoded by the reading frames has domains of biological interest and considerable overlapping molecular functions associated with cellular behavior and cancer progression. Methods: Here we examined molecular features of SPTLC1 in a group of inflammation associated cancer cell lines SKN-SH, MDA-PCa, Glioma LN18, PC3 and 647V. Subcellular localization of SPTLC1 was assessed by immunofluorescence microscopy and recombinant green fluorescent protein expression. In addition, PCR, DNA sequencing and bioinformatics analysis were used for molecular profiling of the SPTLC1 genomic and reverse transcribed cDNA fragments. Results: SPTLC1 is detected in all cell lines examined, with intense peri-nuclear staining, consistent with localization in the cytoplasm. Genomic DNA sample, but not the cD NA of SKN cells could be amplified with an AltORF primer set. The PC3 and MDA-PCa cancer cell lines which are both of prostate origin, show differences in SPTLC1 PCR amplification. Similar levels of SPTLC1 AltORF transcripts were detected by quantitative RT-PCR in all cell lines, except the PC3 cell line with low transcript level whose cDNA did not generate nucleotide base sequence information. Conclusions: This is the first reported transcriptional expression of the SPTLC1 AltORF for the inflammation associated human cancer cell lines. Interestingly, it is proximate of oncogenic cancer susceptibility genes and distal of tumor suppressor genes, the high content of short nucleotide repeats in the SPTLC1 AltORF sequence suggesting the region may be genetically unstable. This nominal functional genomics report on the human SPTLC1 AltORF will contribute to compiling a more detailed SPTLC1 gene ontology and is expected to help shed more insight into unique molecular attributes of SPTLC1 in the context of cancer cell behavior, malignant progression and the design of treatment for inflammation associated cancers. 展开更多
关键词 CYTOGENETIC Band Functional Genomics HUMAN chromosome 9 MICROSATELLITE Instability SERINE Palmitoyltransferase-1
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Dense Fractal Networks, Trends, Noises and Switches in Homeostasis Regulation of Shannon Entropy for Chromosomes’ Activity in Living Cells for Medical Diagnostics
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作者 Nikolay E. Galich 《Applied Mathematics》 2013年第11期30-41,共12页
We analyze correlations and patterns of oxidative activity of 3D DNA at DNA fluorescence in complete sets of chromosomes in neutrophils of peripheral blood. Fluorescence of DNA is registered by method of flow cytometr... We analyze correlations and patterns of oxidative activity of 3D DNA at DNA fluorescence in complete sets of chromosomes in neutrophils of peripheral blood. Fluorescence of DNA is registered by method of flow cytometry with nanometer spatial resolution. Experimental data present fluorescence of many ten thousands of cells, from different parts of body in each population, in various blood samples. Data is presented in histograms as frequency distributions of flashes in the dependence on their intensity. Normalized frequency distribution of information in these histograms is used as probabilistic measure for definition of Shannon entropy. Data analysis shows that for this measure of Shannon entropy common sum of entropy, i.e. total entropy E, for any histogram is invariant and has identical trends of changes all values of E (r) = lnr at reduction of rank r of histogram. This invariance reflects informational homeostasis of chromosomes activity inside cells in multi-scale networks of entropy, for varied ranks r. Shannon entropy in multi-scale DNA networks has much more dense packing of correlations than in “small world” networks. As the rule, networks of entropy differ by the mix of normal D 2 and abnormal D > 2 fractal dimensions for varied ranks r, the new types of fractal patterns and hinges for various topology (fractal dimension) at different states of health. We show that all distributions of information entropy are divided on three classes, which associated in diagnostics with a good health or dominants of autoimmune or inflammatory diseases. This classification based on switching of stability at transcritical bifurcation in homeostasis regulation. We defined many ways for homeostasis regulation, coincidences and switching patterns in branching sequences, the averages of H&ouml;lder for deviations of entropy from homeostasis at different states of health, with various saturation levels the noises of entropy at activity of all chromosomes in support regulation of homeostasis. 展开更多
关键词 Abnormal Fractals DNA ACTIVITY and Shannon Information ENTROPY FRACTAL patterns and Fragmentation Informational HOMEOSTASIS Saturations of CHROMOSOMAL Correlations Multi-Scale FRACTAL NETWORKS of Shannon ENTROPY
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胎儿末端染色体非平衡易位遗传方式的CNV-seq联合G显带核型分析 被引量:2
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作者 侯雅勤 时盼来 +3 位作者 代鹏 陈铎 白莹 孔祥东 《郑州大学学报(医学版)》 CAS 北大核心 2024年第1期50-55,共6页
目的:通过拷贝数变异检测(CNV-seq)联合G显带核型分析对产前诊断和流产的胎儿末端染色体非平衡易位发生频率以及遗传方式进行分析。方法:选取2018年6月至2021年12月在郑州大学第一附属医院经CNV-Seq判定为末端染色体非平衡易位的病例,... 目的:通过拷贝数变异检测(CNV-seq)联合G显带核型分析对产前诊断和流产的胎儿末端染色体非平衡易位发生频率以及遗传方式进行分析。方法:选取2018年6月至2021年12月在郑州大学第一附属医院经CNV-Seq判定为末端染色体非平衡易位的病例,采用外周血G显带核型分析或FISH检测对胎儿父母进行溯源分析。结果:17248例产前诊断和流产病例中,88例检出末端染色体非平衡易位,检出率为0.51%。其中59例行父母G显带核型分析或FISH检测,32例(54.24%)是由于父母为平衡易位导致,27例(45.76%)为新发变异。结论:诊断为末端染色体非平衡易位的病例,父母行G显带核型分析或FISH检测可提高染色体平衡易位携带者的检出率。 展开更多
关键词 拷贝数变异检测 末端染色体非平衡易位 G显带核型分析
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R-Band of Northeastern Sika Deer (Cervus nippon hortulorum) Chromosomes
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作者 JIANG De-mei CAI Zhi-hua +4 位作者 TAO Hong-mei HAN LI JIANG Ji WEN Xlng-fu FAN Ru-quan 《Animal Husbandry and Feed Science》 CAS 2009年第11期1-3,共3页
Objeclive The aim of this study was to investigate R-band of Cervus nippon hortulorum chromosomes and to provide references for genetic variation and gene location of Cervus nippon hortulorum. [Metbod] Cell division w... Objeclive The aim of this study was to investigate R-band of Cervus nippon hortulorum chromosomes and to provide references for genetic variation and gene location of Cervus nippon hortulorum. [Metbod] Cell division was synchronized by the pepripheral blood lymphocyte culture and the excessive dosage of thymine deoxyribonucleoside, and R-band of Cervus nippon hortulorum chromosomes was also analyzed by RBG-banding technique. Result The number of haploid chromosome banding increased to 400. The R-band of No. 1, No. 2, No. 3, No. 4, chromosome X and Y were almost just opposite to the high-resolution G band of them. The terminal of chromosomes except No. 21, No. 24 and No. 28 were all pos- itive deeply stained. E Conclusion] R-band of Cervus nippon hortulorum chromosomes can be manifested by RBG-binding technique. 展开更多
关键词 Cervus nippon hortulorum chromosome R-band
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奶牛小肠上皮细胞系细胞染色体核型与G-带分析
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作者 王雪莹 孟素丹 +5 位作者 何雷 赵淑娟 王聪慧 李日顺 钱伟锋 张才 《河南科技大学学报(自然科学版)》 CAS 北大核心 2024年第4期73-78,M0007,共7页
为了鉴定奶牛小肠上皮细胞系(BIECs-21)的生物学遗传稳定性,体外贴壁培养BIECs-21细胞,制备染色体标本和G-带标本,分析BIECs-21细胞的染色体核型和G-带。结果表明:BIECs-21细胞染色体数为2n=60,包括常染色体29对和性染色体(X和Y染色体)... 为了鉴定奶牛小肠上皮细胞系(BIECs-21)的生物学遗传稳定性,体外贴壁培养BIECs-21细胞,制备染色体标本和G-带标本,分析BIECs-21细胞的染色体核型和G-带。结果表明:BIECs-21细胞染色体数为2n=60,包括常染色体29对和性染色体(X和Y染色体)1对。其中,29对常染色体均为端部着丝点染色体;性染色体中,X染色体是较大的中着丝粒染色体,Y染色体是较小的中着丝粒染色体。除X、Y性染色体外,BIECs-21细胞常染色体的G-带带纹的数量和位置无显著差异。BIECs-21细胞与荷斯坦牛的正常染色体核型相比,染色体数目及形态结构均没有明显变化,证明该细胞系遗传特性稳定,可以用于牛肠道相关疾病分子机制的研究。 展开更多
关键词 BIECs-21细胞 贴壁培养 染色体核型分析 G-带分析
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染色体微阵列分析在580例胎儿超声异常产前诊断中的应用价值
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作者 林堃 黄洁香 +3 位作者 胡聪颖 黄敏君 林荔 陈明桥 《福建医科大学学报》 2024年第3期206-212,共7页
目的探讨染色体微阵列分析技术(CMA)在不同种类胎儿超声异常产前诊断中的应用价值。方法回顾性分析2020年1月—2023年12月因胎儿超声检查异常在产前诊断中心同时行G显带染色体核型分析和CMA检测的580例羊水样本的检测结果,随访CMA阳性... 目的探讨染色体微阵列分析技术(CMA)在不同种类胎儿超声异常产前诊断中的应用价值。方法回顾性分析2020年1月—2023年12月因胎儿超声检查异常在产前诊断中心同时行G显带染色体核型分析和CMA检测的580例羊水样本的检测结果,随访CMA阳性病例的妊娠结局。结果580例中,CMA检测阳性90例,阳性率15.5%,较染色体核型分析的阳性率(34例,5.9%)提高了9.6%(χ^(2)=28.3,P<0.001)。580例中,超声结构异常167例,其中CMA检测阳性25例(阳性率15.0%),腹部异常以及呼吸、骨骼、神经和心血管等系统异常的阳性率分别为33.3%、29.4%、16.7%、16.7%和14.3%;超声非结构异常413例,其中CMA检测阳性65例(阳性率15.7%),多发软指标异常、孤立软指标中的脉络丛囊肿和颈项透明层(NT)增厚阳性率依次为23.9%、20.0%和18.6%。随访90例CMA阳性病例的妊娠结局,其中正常活产42例,引产36例,胎停2例,出生后异常5例,未出生5例。结论在胎儿超声异常的产前诊断中,CMA技术可弥补染色体核型分析技术的局限性;超声检查发现胎儿存在孤立/多发结构异常或超声软指标异常时,推荐行CMA检测;临床意义不明的拷贝数变异需要结合本地随访结局数据并查阅最新文献进行遗传咨询。 展开更多
关键词 染色体微阵列分析 超声异常 G显带染色体核型分析
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论云冈第13窟窟顶图像的来源与传播
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作者 王韦韬 《艺术探索》 CSSCI 2024年第4期78-85,共8页
云冈第13窟窟顶图像由依附于主尊身光上方的弧状纹样带与两条交缠的龙组成。窟顶依附于主尊身光上缘的弧状纹样带,最早可追溯至犍陀罗、秣菟罗地区佛教造像中尊像身后的树木,被称为“华盖式圣树”。此窟窟顶纹样带内部忍冬枝藤所结果实... 云冈第13窟窟顶图像由依附于主尊身光上方的弧状纹样带与两条交缠的龙组成。窟顶依附于主尊身光上缘的弧状纹样带,最早可追溯至犍陀罗、秣菟罗地区佛教造像中尊像身后的树木,被称为“华盖式圣树”。此窟窟顶纹样带内部忍冬枝藤所结果实或代表了兜率天中“果如颇黎色”的七宝行树,与同样位于窟顶的交龙(或为护树、为树布雨的龙王)共同构成了兜率天胜景。豫北北魏背屏式造像侧面纹饰及龙门石窟北魏晚期圆楣龛圆楣、部分造像龛主尊内层身光顶部的忍冬纹都与云冈第13窟窟顶图像存在不同程度的亲缘关系。 展开更多
关键词 圣树 弧状纹样带 忍冬纹
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400例性发育异常患者遗传学病因检测与特征分析
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作者 孙夏瑜 武坚锐 +4 位作者 卢洪涌 叶婷 郭荣 孙畅 薛慧琴 《检验医学与临床》 CAS 2024年第23期3537-3540,共4页
目的应用多种遗传学技术检测诊断400例性发育异常(DSD)患者的遗传学病因,并总结分析其临床特征。方法选取2018年1月至2023年3月该院收治的400例确诊为DSD的患者作为研究对象,运用G-显带染色体核型分析、荧光原位杂交(FISH)、多重链接探... 目的应用多种遗传学技术检测诊断400例性发育异常(DSD)患者的遗传学病因,并总结分析其临床特征。方法选取2018年1月至2023年3月该院收治的400例确诊为DSD的患者作为研究对象,运用G-显带染色体核型分析、荧光原位杂交(FISH)、多重链接探针扩增技术(MLPA)、高通量测序的低深度全基因组测序(CNV-seq)、全外显子组测序技术(WES)、全基因组测序(WGS)等技术进行检测和定位,查找患者遗传学病因。结果经G-显带染色体核型分析,以及FISH、MLPA对核型分析结果进行验证,明确诊断45,X特纳综合征及其变异型136例(34.00%),47,XXY克氏综合征及其变异型187例(46.75%);经G-显带染色体核型分析、CNV-seq、WES、WGS、一代测序技术诊断46,XX DSD 17例(4.25%)、46,XY DSD 35例(8.75%);经G-显带染色体核型分析和FISH、MLPA验证诊断性染色体核型异常DSD 25例(6.25%)。结论联合应用G-显带染色体核型、FISH、CNV-seq、WES等多种遗传学方法,能准确发现DSD的遗传学病因,建立基因型与表现型之间的良好关系。 展开更多
关键词 性发育异常 遗传学病因 G-显带染色体核型分析 低深度全基因组测序 全外显子组测序技术
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分子诊断技术在先天性泌尿系统发育异常胎儿中的应用
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作者 黎昱 宋婷婷 +3 位作者 郑娇 徐盈 李佳 杨红 《空军军医大学学报》 CAS 2024年第6期661-665,共5页
目的探讨联合采用G显带核型分析和染色体微阵列(CMA)分析技术在产前超声筛查提示先天性泌尿系统发育异常(CAKUT)胎儿遗传学检测中的应用价值,分析CAKUT胎儿的发病机制及遗传背景。方法回顾性分析2017年1月至2022年12月在空军军医大学西... 目的探讨联合采用G显带核型分析和染色体微阵列(CMA)分析技术在产前超声筛查提示先天性泌尿系统发育异常(CAKUT)胎儿遗传学检测中的应用价值,分析CAKUT胎儿的发病机制及遗传背景。方法回顾性分析2017年1月至2022年12月在空军军医大学西京医院妇产科因CAKUT进行遗传咨询并进行了侵入性产前诊断的519例胎儿样本,联合采用G显带核型分析和CMA对采集得到的样本进行检测,对超声结果、遗传学检测结果进行分析,对妊娠结局进行随访。结果519例样本中8例检出染色体异常,均为数目异常(3例为常染色体数目异常,5例为性染色体数目异常),检出可能致病性拷贝数变异(CNVs)11例,检出致病性CNVs 8例,总阳性检出率为5.2%。CNVs中最常见的为17q12微缺失综合征,共检出4例。结论CAKUT是产前超声中一类常见的结构畸形,采用G显带核型分析和CMA的联合应用可以提高染色体异常的检出率,为遗传咨询、妊娠结局和再生育提供准确的依据。 展开更多
关键词 先天性泌尿系统发育异常 产前诊断 G显带核型分析 染色体微阵列分析 17q12微缺失综合征
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单核苷酸微阵列芯片技术联合染色体核型分析在产前诊断中的应用
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作者 黄霜 陈素琴 《广州医科大学学报》 2024年第2期30-35,共6页
目的:评估G显带染色体核型分析与单核苷酸多态性微阵列芯片(single nucleotide polymorphism array,SNParray)同时检测在羊水产前诊断中的应用,为临床诊断与遗传咨询提供依据。方法:选取837例具有产前诊断指征而行产前诊断的孕妇,知情... 目的:评估G显带染色体核型分析与单核苷酸多态性微阵列芯片(single nucleotide polymorphism array,SNParray)同时检测在羊水产前诊断中的应用,为临床诊断与遗传咨询提供依据。方法:选取837例具有产前诊断指征而行产前诊断的孕妇,知情同意后采集羊水行染色体G显带核型分析和SNParray检测,分析检测结果。结果:核型分析、SNParray技术和联合应用的异常检出率分别为11.11%、13.14%和14.70%。依据不同的指征分组,染色体核型分析和SNParray的异常率,无创产前检测(NIPT)高危组最高,胎儿超声异常组次之。SNParray全部检出核型分析发现的59例胎儿染色体数目异常;SNParray在30例核型分析漏诊的胎儿中检测出拷贝数异常(CNV);此外,SNParray能够识别未知片段的来源。SNParray检出的10例嵌合体,全部与核型分析结果一致。核型分析异常而SNParray漏检的有10例,其中8例为平衡异位或倒位,2例为嵌合体。联合分析检出异常的123例胎儿中,经过遗传咨询,79例家属选择终止妊娠,44例家属选择继续妊娠;选择继续妊娠的,44例顺利出生;这些出生的病例均随访至婴儿出生后1年,暂未发现与产前诊断结果不符的病例。结论:G显带染色体核型分析与SNParray技术各有优缺点,联合应用可明显提高产前诊断中染色体异常的检出率,从而为临床诊断与遗传咨询提供更好的指导。 展开更多
关键词 产前诊断 G显带染色体核型分析 单核苷酸微阵列芯片技术 羊水
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带夹装置在高精度刺绣中的应用与性能优化
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作者 章海明 《纺织报告》 2024年第8期9-11,共3页
高精度刺绣在现代纺织工业中占据重要地位,但传统带夹装置难以精确调节夹持力,导致绣带移位和刺绣质量下降。文章提出了一种新型带夹装置,通过改进结构和功能,提升刺绣精度和生产效率。该装置利用压簧和螺帽的调节机制,实现绣带的良好... 高精度刺绣在现代纺织工业中占据重要地位,但传统带夹装置难以精确调节夹持力,导致绣带移位和刺绣质量下降。文章提出了一种新型带夹装置,通过改进结构和功能,提升刺绣精度和生产效率。该装置利用压簧和螺帽的调节机制,实现绣带的良好固定和夹持力的精确调节,适用于不同厚度和材质的绣带。实验结果表明,使用新型带夹装置后,刺绣精度、绣带稳定性、生产效率、操作便捷性等指标均显著优于未使用装置的情况,减少了图案偏移和失真,提升了整体质量。性能优化措施包括选择高强度材料、改进结构设计和定期维护,延长了装置的使用寿命,提高了装置的工作效率,对提升纺织工业技术水平和产品质量具有重要意义。 展开更多
关键词 带夹装置 高精度刺绣 现代纺织 绣带移位 图案跑偏
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鹌鹑的核型及G带分析 被引量:8
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作者 徐琪 陈国宏 +4 位作者 张学余 王偕根 嵇宝华 李碧春 吴信生 《遗传》 CAS CSCD 北大核心 2004年第6期865-869,共5页
分别采用外周血淋巴细胞培养法和胰酶处理法 ,对鹌鹑染色体的核型和G带进行了研究。结果表明 ,鹌鹑染色体数目为 2n =78,有 10对大染色体 (包括Z、W ) ,2 9对小染色体。染色体形态分别为 :NO .1染色体为sm型 ,NO .2、Z染色体为m型 ,其... 分别采用外周血淋巴细胞培养法和胰酶处理法 ,对鹌鹑染色体的核型和G带进行了研究。结果表明 ,鹌鹑染色体数目为 2n =78,有 10对大染色体 (包括Z、W ) ,2 9对小染色体。染色体形态分别为 :NO .1染色体为sm型 ,NO .2、Z染色体为m型 ,其余染色体均为t型 ,这与前人研究结果存在一定差异。G带分析结果显示 ,鹌鹑的前 9对大染色体及Z、W染色体G带可分为 2 7个区 。 展开更多
关键词 鹌鹑 染色体 核型 G带
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应用G显带染色体荧光原位杂交(FISH)技术研究复杂的染色体易位 被引量:18
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作者 黄浩杰 张锡然 +1 位作者 陈宜峰 崔英霞 《Acta Genetica Sinica》 SCIE CAS CSCD 1996年第5期338-342,共5页
建立常规G显带染色体标本的荧光原位杂交(FISH)技术,用于分析患者复杂的染色体易位。原位杂交前,用甲醛固定G显带标本,是获得良好显带和荧光杂交效果的关键步骤。仅用常规细胞遗传学方法分析,显示一例习惯性流产患者的核型... 建立常规G显带染色体标本的荧光原位杂交(FISH)技术,用于分析患者复杂的染色体易位。原位杂交前,用甲醛固定G显带标本,是获得良好显带和荧光杂交效果的关键步骤。仅用常规细胞遗传学方法分析,显示一例习惯性流产患者的核型为46,XX,t(1;5;12)(1pter→1q25::12q24→12qter;5qter→5p11::1q25→1qter,12pter→12q24:.5p11→5pter),而采用本方法确定患者的核型实际为46,XX,t(1;5,12)(1pter→1q23::12q22→12qter,5qter→5p11::1q25→1qter;12pter→12q22::1q23→1q25:5p11→5pter)。结果表明,新建立的G显带染色体荧光原位杂交(FISH)技术能更有效地检测患者复杂的染色体易位。 展开更多
关键词 染色体 易位 荧光原位杂交 染色体G显带
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仙居鸡染色体G带和Ag-NORs的研究 被引量:12
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作者 陈国宏 李碧春 +4 位作者 徐琪 张学余 吴信生 刘莉 嵇宝华 《畜牧兽医学报》 CAS CSCD 北大核心 2004年第2期141-145,共5页
运用改进的鸡外周血淋巴细胞培养———空气干燥法,分析了仙居鸡染色体G带和Ag NORs。G带研究结果表明:前10对大型染色体可分为30个区,144条带,同时对G带带型特征进行了具体描述,并绘制了G带模式图。Ag NORs处理发现:仙居鸡的Ag NORs常... 运用改进的鸡外周血淋巴细胞培养———空气干燥法,分析了仙居鸡染色体G带和Ag NORs。G带研究结果表明:前10对大型染色体可分为30个区,144条带,同时对G带带型特征进行了具体描述,并绘制了G带模式图。Ag NORs处理发现:仙居鸡的Ag NORs常分布于1p、3p、4p、Zp、2q上,均数为3.26,众数为3。不同个体间以及同一个体的不同细胞间Ag NORs数目存在多态性;此外研究还发现Ag NORs联合现象。 展开更多
关键词 仙居鸡 染色体 G带 AG-NORS 血淋巴细胞 细胞培养 空气干燥法
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三种姬鼠的染色体比较研究 被引量:8
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作者 陈志平 刘瑞清 +1 位作者 李崇云 王应祥 《Zoological Research》 CAS CSCD 1996年第3期347-352,共6页
本文采用染色体分带技术(G-,C-带和银染色),对中华姬鼠(Apodemusdraco)、大林姬鼠(A.peninsulae)和大耳姬鼠(A.latronum)的核型进行了观察分析。结果表明:3种姬鼠的2n均为48。... 本文采用染色体分带技术(G-,C-带和银染色),对中华姬鼠(Apodemusdraco)、大林姬鼠(A.peninsulae)和大耳姬鼠(A.latronum)的核型进行了观察分析。结果表明:3种姬鼠的2n均为48。中华姬鼠的染色体均为端着丝点染色体。大林姬鼠的常规核型中,除1对中着丝点染色体(No.23)外,其余均为端着丝点染色体。大耳姬鼠的核型中,有13对端着丝点染色体,2对亚端着丝点染色体,1对亚中着丝点染色体和7对中着丝点染色体。中华姬鼠C-带核型中,所有染色体着丝点C-带都呈强阳性,异染色质非常丰富,Y染色体整条深染。在大林姬鼠C-带核型中,Nos.7,11,15,21,22着丝点C-带弱化甚至近阴性,其余染色体着丝点异染色质C-带都呈现程度不同的阳性。且Nos.2,4,7有强弱不同的端位异染色质带。X染色体着丝点区有大块的异染色质斑带出现,Y染色体整条深染。大耳姬鼠除Nos.3,4,10,12,13染色体着丝点C-带很弱外,其余染色体着丝点C-带均呈阳性,并有8对(Nos.16-23)染色体出现异染色质短臂。从总体上看,大林姬鼠和大耳姬鼠的着丝点异染色质明显比中华姬鼠的少。中华姬鼠的Ag-NOR? 展开更多
关键词 姬鼠 染色体 啮齿目
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