This study was carried out to analyze the vertical transmission of Yq AZFc microdeletions from father to son in infertile Han Chinese families to investigate genetic factors and family background affecting fertility s...This study was carried out to analyze the vertical transmission of Yq AZFc microdeletions from father to son in infertile Han Chinese families to investigate genetic factors and family background affecting fertility status.The peripheral blood of infertile males in 19 Han families was extracted and screened with modified multiplex polymerase chain reaction (PCR). Family trees were drawn according to fertility status and clinical characteristics of the subjects. The vertical transmission of Yq AZFc microdeletions was detected in six cases of 19 investigated families (31.6%,6/19). Although both fathers and sons showed a similar type of Yq AZFc deletion,the fathers were fertile,whereas the sons were infertile and showed severe oligozoospermia. The vertical transmission of Yq AZFc microdeletion from fertile fathers to infertile sons over generations is not rare. This has different effects on fertility status in fathers and sons in Han Chinese families. Both genetic factors and family background affect spermatogenetic phenotypes.展开更多
[ Objective ] This study aimed to provide a scientific basis for breeding new sugarcane varieties with hybrid progenies of Erianthus fulvus and modifying existing sugarcane cultivars. [ Method] Chromosomal slides of t...[ Objective ] This study aimed to provide a scientific basis for breeding new sugarcane varieties with hybrid progenies of Erianthus fulvus and modifying existing sugarcane cultivars. [ Method] Chromosomal slides of the F2 and F2 BC1 hybrid progenies between sugarcane and E. fu/vus (2n = 20) and those of parental materials were prepared with the wall degradation hypotonic method, to further analyze the chromosomal transmission in the F2 and F2BC1 hybrid progenies and to investigate the chromosome transmission mechanism. [ Result ] The results showed that, somatic chromosome number varied in various hybrid progenies; chromo- somes from parents were transmitted into the somatic cells of F2 hybrids in "2n + n" pattern and transmitted into the somatic cells of F2BC1 hybrids in "n + n" pattern. [ Conclusion ] This study provided a preliminary cytological basis for further use of these materials in sugarcane breeding.展开更多
文摘This study was carried out to analyze the vertical transmission of Yq AZFc microdeletions from father to son in infertile Han Chinese families to investigate genetic factors and family background affecting fertility status.The peripheral blood of infertile males in 19 Han families was extracted and screened with modified multiplex polymerase chain reaction (PCR). Family trees were drawn according to fertility status and clinical characteristics of the subjects. The vertical transmission of Yq AZFc microdeletions was detected in six cases of 19 investigated families (31.6%,6/19). Although both fathers and sons showed a similar type of Yq AZFc deletion,the fathers were fertile,whereas the sons were infertile and showed severe oligozoospermia. The vertical transmission of Yq AZFc microdeletion from fertile fathers to infertile sons over generations is not rare. This has different effects on fertility status in fathers and sons in Han Chinese families. Both genetic factors and family background affect spermatogenetic phenotypes.
基金Supported by National Natural Science Foundation of China"Study on Chromosomal Transmission Pattern and Specific DNA Sequence Mapping in Erianthus fulvus Hybrids(30960045)Program for Construction of Modern Agricultural Sugarcane Industry Technology System of Yunnan Province"Breeding and Reproduction Laboratory Post"(YCJ[2013]No.160)+2 种基金Key Project of New Product Development of Yunnan Province"Breeding and Industrial Development of New Varieties of High-yield and High-sugar Ratoon Sugarcane"(Agriculture,2012BB014)Innovation Team Project for Sustainable Production System of Mountain Plateau Crops of Yunnan Agricultural University"Innovation and Utilization of Special Mountain Plateau Crop Germplasms"(YKRF[2012]No.18)Fund of Education Department of Yunnan Province"Genetic Diversity of Sugarcane Wild Species Erianthus fulvus"(2011C174)
文摘[ Objective ] This study aimed to provide a scientific basis for breeding new sugarcane varieties with hybrid progenies of Erianthus fulvus and modifying existing sugarcane cultivars. [ Method] Chromosomal slides of the F2 and F2 BC1 hybrid progenies between sugarcane and E. fu/vus (2n = 20) and those of parental materials were prepared with the wall degradation hypotonic method, to further analyze the chromosomal transmission in the F2 and F2BC1 hybrid progenies and to investigate the chromosome transmission mechanism. [ Result ] The results showed that, somatic chromosome number varied in various hybrid progenies; chromo- somes from parents were transmitted into the somatic cells of F2 hybrids in "2n + n" pattern and transmitted into the somatic cells of F2BC1 hybrids in "n + n" pattern. [ Conclusion ] This study provided a preliminary cytological basis for further use of these materials in sugarcane breeding.