BACKGROUND Innate immunity was found to be associated with both persistence of Helicobacter pylori(H.pylori)infection and increased risk of gastric cancer.AIM To identify the risk factors associated with H.pylori infe...BACKGROUND Innate immunity was found to be associated with both persistence of Helicobacter pylori(H.pylori)infection and increased risk of gastric cancer.AIM To identify the risk factors associated with H.pylori infection and to establish the role of TLR9 rs352140 in suppressing or promoting inflammation related to this infection in children.METHODS We performed a study of 155 children with digestive symptoms,who were divided into two groups according to the histopathological exam:Group 1–48 children with H.pylori-induced chronic gastritis,and Group 2–control group.RESULTS Rural area and poor living conditions were significantly associated with H.pylori chronic gastritis(P=0.0042/P<0.0001).Both positive immunoglobulin A anti H.pylori and the rapid urease test were significantly associated with H.pylori infection(P<0.0001).Significantly higher values of leukocytes and neutrophils within the peripheral blood were found in children with H.pylori chronic gastritis(P=0.111/P=0.284).We found a significant positive correlation between the variant TT genotype of TLR9 rs352140 polymorphism and both leucocytes and neutrophils(P=0.0225/P=0.0292).CONCLUSION Variant TT genotype carriers of the TLR9 rs352140 gene polymorphism might have a more severe degree of inflammation.展开更多
Importance:Allogeneic hematopoietic stem cell transplantation(alloHSCT)is considered the only effective treatment for chronic active Epstein–Barr virus infection(CAEBV).The clinical efficacy and safety of allo-HSCT w...Importance:Allogeneic hematopoietic stem cell transplantation(alloHSCT)is considered the only effective treatment for chronic active Epstein–Barr virus infection(CAEBV).The clinical efficacy and safety of allo-HSCT with different conditioning regimens in children with CAEBV remain unclear.Objective:To evaluate the effectiveness and safety of allo-HSCT with the modified myeloablative conditioning(MAC)regimen for children with CAEBV and also the factors affecting the outcomes.Methods:We retrospectively analyzed children with CAEBV who underwent allo-HSCT with the modified MAC regimen at Beijing Children’s Hospital,Capital Medical University from October 2016 to June 2021.Data related to the clinical manifestations,engraftment,and outcome were extracted from the medical records.Results:The cohort comprised 41 patients(24 males,17 females)with a median transplantation age of 92.6(60.4,120.7)months and a median follow-up time of 28.2(15.3,40.2)months.Four patients(9.8%)died,among which three died from primary disease relapse,and one died from grade IV acute graft-versus-host diseases(aGVHD)after stopping treatment.The 3-year overall survival(OS)and 3-year event-free survival(EFS)rates were 88.8%±5.4%and 85.0%±5.7%,respectively.The 3-year OS and EFS did not significantly differ between the patients with hemophagocytic lymphohistiocytosis(HLH)and the patient without HLH(87.7%±6.8%vs.91.7%±8.0%,P=0.790;85.0%±6.9%vs.84.6%±10.0%,P=0.921),or among the patients with complete remission,partial remission,and activity disease before HSCT(all P>0.05).Multivariate analysis showed that grade III–IV aGVHD was a risk factor for mortality(Hazards ratio:11.65,95%confidence interval:1.00,136.06;P=0.050).Interpretation:Allo-HSCT with the modified MAC regimen is safe and effective for pediatric CAEBV.This treatment benefits patients with HLH or active disease.Patients with Grade III–IV aGVHD may be associated with worse outcomes.展开更多
Background: The common cause of dental infections is necrosis of the pulp of a tooth, which is followed by bacterial invasion through the pulp chamber into the deeper tissues. The bacterial infections are caused by ho...Background: The common cause of dental infections is necrosis of the pulp of a tooth, which is followed by bacterial invasion through the pulp chamber into the deeper tissues. The bacterial infections are caused by host indigenous bacteria primarily aerobic gram positive cocci, anaerobic gram negative and gram negative rods. The aim of this study is to identify the presence of different cultivating microorganisms which cause dental infections in primary and young permanent teeth manifested by as abscesses and apical parodontitis. Material and Methods: Seventy kids ages 5 - 15 years ( 36 females, 34 males) were studied prospectively (I assume this) at the University Dental Clincal Center of Kosovo, Department of Pediatric Dentistry in cooperation with Microbiological Department of Faculty of Medicine. All patients who had clinical, and radiological confirmation of thier dental infections, followed by extraction of their teeth. Once the tooth was extracted blood and pus sample from the empty alveoli were cultured. The analysis of the sample for bacteria identification was done using the automated system VITEK 2 cards (Biomeriux, France). Results: Of 70 children’s with deciduous 73.5% of them had acute infection and 26.5% chronic infection. On the other hand 75% of children with permanent dentitions had chronic and 25% acute infection. In acute infections the most common aerobic bacteria was Streptococcus group with 47% (Streptococcus mitis and oralis) followed by the Actonomyces group (Actinomyces mayeri and Actinomyces odontoliticus) and anaerobic bacteria in 34%. In chronic infections streptococcus group was the prevalent aerobic group and in anaerobic infection Actinomyces mayeri and Actinomyces naeslundi was the most representative aerobes in 21% of samples. Conclusion: The dental infections in pediatric population (5 - 15 years old) are polymicrobial predominantly anaerobic bacteria over aerobic. This study paves the way for preventives measures that need to me implemented in this group of children.展开更多
Background:Available pediatric treatments for acute cough are limited by lack of demonstrated efficacy.The objective of this trial is to compare the effects of a poIysaccharide—resin-honey based cough syrup,and carbo...Background:Available pediatric treatments for acute cough are limited by lack of demonstrated efficacy.The objective of this trial is to compare the effects of a poIysaccharide—resin-honey based cough syrup,and carbocysteine syrups on nocturnal and daytime cough associated with childhood upper respiratory tract infections(URIs).展开更多
基金Supported by University of Medicine,Pharmacy,Sciences and Technology“George Emil Palade”of Târgu Mureş,Romania,No.293/6/14.01.2020.
文摘BACKGROUND Innate immunity was found to be associated with both persistence of Helicobacter pylori(H.pylori)infection and increased risk of gastric cancer.AIM To identify the risk factors associated with H.pylori infection and to establish the role of TLR9 rs352140 in suppressing or promoting inflammation related to this infection in children.METHODS We performed a study of 155 children with digestive symptoms,who were divided into two groups according to the histopathological exam:Group 1–48 children with H.pylori-induced chronic gastritis,and Group 2–control group.RESULTS Rural area and poor living conditions were significantly associated with H.pylori chronic gastritis(P=0.0042/P<0.0001).Both positive immunoglobulin A anti H.pylori and the rapid urease test were significantly associated with H.pylori infection(P<0.0001).Significantly higher values of leukocytes and neutrophils within the peripheral blood were found in children with H.pylori chronic gastritis(P=0.111/P=0.284).We found a significant positive correlation between the variant TT genotype of TLR9 rs352140 polymorphism and both leucocytes and neutrophils(P=0.0225/P=0.0292).CONCLUSION Variant TT genotype carriers of the TLR9 rs352140 gene polymorphism might have a more severe degree of inflammation.
基金Beijing Municipal Science&Technology Commission,Grant/Award Number:Z171100001017050National Science and Technology Key Projects,Grant/Award Number:2017ZX09304029。
文摘Importance:Allogeneic hematopoietic stem cell transplantation(alloHSCT)is considered the only effective treatment for chronic active Epstein–Barr virus infection(CAEBV).The clinical efficacy and safety of allo-HSCT with different conditioning regimens in children with CAEBV remain unclear.Objective:To evaluate the effectiveness and safety of allo-HSCT with the modified myeloablative conditioning(MAC)regimen for children with CAEBV and also the factors affecting the outcomes.Methods:We retrospectively analyzed children with CAEBV who underwent allo-HSCT with the modified MAC regimen at Beijing Children’s Hospital,Capital Medical University from October 2016 to June 2021.Data related to the clinical manifestations,engraftment,and outcome were extracted from the medical records.Results:The cohort comprised 41 patients(24 males,17 females)with a median transplantation age of 92.6(60.4,120.7)months and a median follow-up time of 28.2(15.3,40.2)months.Four patients(9.8%)died,among which three died from primary disease relapse,and one died from grade IV acute graft-versus-host diseases(aGVHD)after stopping treatment.The 3-year overall survival(OS)and 3-year event-free survival(EFS)rates were 88.8%±5.4%and 85.0%±5.7%,respectively.The 3-year OS and EFS did not significantly differ between the patients with hemophagocytic lymphohistiocytosis(HLH)and the patient without HLH(87.7%±6.8%vs.91.7%±8.0%,P=0.790;85.0%±6.9%vs.84.6%±10.0%,P=0.921),or among the patients with complete remission,partial remission,and activity disease before HSCT(all P>0.05).Multivariate analysis showed that grade III–IV aGVHD was a risk factor for mortality(Hazards ratio:11.65,95%confidence interval:1.00,136.06;P=0.050).Interpretation:Allo-HSCT with the modified MAC regimen is safe and effective for pediatric CAEBV.This treatment benefits patients with HLH or active disease.Patients with Grade III–IV aGVHD may be associated with worse outcomes.
文摘Background: The common cause of dental infections is necrosis of the pulp of a tooth, which is followed by bacterial invasion through the pulp chamber into the deeper tissues. The bacterial infections are caused by host indigenous bacteria primarily aerobic gram positive cocci, anaerobic gram negative and gram negative rods. The aim of this study is to identify the presence of different cultivating microorganisms which cause dental infections in primary and young permanent teeth manifested by as abscesses and apical parodontitis. Material and Methods: Seventy kids ages 5 - 15 years ( 36 females, 34 males) were studied prospectively (I assume this) at the University Dental Clincal Center of Kosovo, Department of Pediatric Dentistry in cooperation with Microbiological Department of Faculty of Medicine. All patients who had clinical, and radiological confirmation of thier dental infections, followed by extraction of their teeth. Once the tooth was extracted blood and pus sample from the empty alveoli were cultured. The analysis of the sample for bacteria identification was done using the automated system VITEK 2 cards (Biomeriux, France). Results: Of 70 children’s with deciduous 73.5% of them had acute infection and 26.5% chronic infection. On the other hand 75% of children with permanent dentitions had chronic and 25% acute infection. In acute infections the most common aerobic bacteria was Streptococcus group with 47% (Streptococcus mitis and oralis) followed by the Actonomyces group (Actinomyces mayeri and Actinomyces odontoliticus) and anaerobic bacteria in 34%. In chronic infections streptococcus group was the prevalent aerobic group and in anaerobic infection Actinomyces mayeri and Actinomyces naeslundi was the most representative aerobes in 21% of samples. Conclusion: The dental infections in pediatric population (5 - 15 years old) are polymicrobial predominantly anaerobic bacteria over aerobic. This study paves the way for preventives measures that need to me implemented in this group of children.
文摘Background:Available pediatric treatments for acute cough are limited by lack of demonstrated efficacy.The objective of this trial is to compare the effects of a poIysaccharide—resin-honey based cough syrup,and carbocysteine syrups on nocturnal and daytime cough associated with childhood upper respiratory tract infections(URIs).
