AIM: To compare the prevalence of Helicobacter pylori (Hpylori) IgG and IgA antibodies between adult subjects,with defined gastric diseases, nondefined gastric disorders and those representing the population.METHODS: ...AIM: To compare the prevalence of Helicobacter pylori (Hpylori) IgG and IgA antibodies between adult subjects,with defined gastric diseases, nondefined gastric disorders and those representing the population.METHODS: Data on H pylori IgG and IgA antibodies,determined by enzyme immunoassay, were analyzed in 3 252 subjects with DGD including 482 patients with gastric ulcer, 882 patients with duodenal ulcer, 1 525patients with chronic gastritis only and 363 subjects with subsequent gastric cancer, 19 145 patients with NoDg and4 854 POPUL subjects. The age-adjusted prevalences were calculated for 1- and 20-year age cohorts.RESULTS: The prevalences of IgG antibodies were equally high (89-96%) in all 20-year age cohorts of the DGD groups, whereas the prevalences of IgG antibodies were lower and increased by age in the POPUL and NoDg groups. The prevalences of IgA antibodies were also higher in the DGD groups; among them CA (84-89%) and GU groups (78-91%) showed significantly higher prevalences than DU (68-77%) and CG patients (59-74%) (OR 2.49, 95%CI 1.86-3.34 between the GU and DU groups). In the CA, GU, and DU groups, the IgA prevalences showed only minor variation according to age, while they increased by age in the CG, POPUL, and NoDg groups (P≤0.0001). The IgA response, but not the IgG response, was associated with an increased risk of CA (OR 2.41, 95%CI 1.79-3.53) and GU (OR 2.57,95%CI 1.95-3.39) in comparison with CG patients.CONCLUSION: An IgA antibody response during H pylori infection is significantly more common in CA and GU patients as compared with CG patients.展开更多
AIM： To investigate the role of gastric mucosa at the secretion of sTREM-1 in peptic ulcer.METHODS： Seventy two patients were enrolled; 35 with duodenal, 22 with gastric ulcer and 26 with chronic gastritis. Patients...AIM： To investigate the role of gastric mucosa at the secretion of sTREM-1 in peptic ulcer.METHODS： Seventy two patients were enrolled; 35 with duodenal, 22 with gastric ulcer and 26 with chronic gastritis. Patients were endoscoped and gastric juice was aspirated. Patients with duodenal and gastric ulcer underwent a second endoscopy post-treatment. Biopsies were incubated in the absence/presence of endotoxins or gastric juice. Supernatants were collected and sTREM-2 and TNF~ were measured by enzyme immunoabsorbent assays. Scoring of gastritis was performed before and after treatment according to updated Sydney score.RESULTS： Patients with duodenal and gastric ulcer and those with chronic gastritis had similar scores of gastritis, sTREM-1 was higher in supernatants of tissue samples of H pylori-positive than of H pylori-negative patients with gastric ulcer. Median （± SE） sTREM-1 was found increased in supernatants of patients with gastric ulcer before treatment （203.21 ± 88.91 pg/1000 cells） compared to supernatants either from the same patients post-treatment （8.23 ± 5.79 pg/1000 cells） or from patients with chronic gastritis （6.21 ± 0.71 pg/1000 cells） （P 〈 0.001 and 〈 0.001, respectively）. Similar differences for sTREM-1 were recorded among LPS-stimulated tissue samples of patients （P = 0.001）. Similar differences were not found for TNFα. Positive correlations were found between sTREM-1 of supernatants from patients with both duodenal and gastric ulcer before treatment and the degree of infiltration of neutrophils and monocytes.CONCLUSION： sTREM-1 secreted by the gastric mucosa is an independent mechanism connected to the pathogenesis of peptic ulcer, sTREM-1 was released at the presence of H pylori from the inflamed gastric mucosa in the field of gastric ulcer.展开更多
H. pylori infection is mainly spread in the kind of gastroduodenal diseases: chronic gastritis, peptic ulcer disease, MALT-lymphoma, gastric cancer. According to certain literature, the mentioned bacterium causes dise...H. pylori infection is mainly spread in the kind of gastroduodenal diseases: chronic gastritis, peptic ulcer disease, MALT-lymphoma, gastric cancer. According to certain literature, the mentioned bacterium causes diseases of other visceral organs of humans. Study of the aggravating impact of this infection is under the attention of the scientists. However, other infectious agents, including fungi, other bacteria, parasites, and viruses and their role in different gastroduodenal diseases are not studied enough. The aim of our study was to identify mucous (parietal) gastroduodenal microflora in patients with different diseases of this zone. 390 patients with chronic gastritis (CG), peptic ulcer diseases (PUD) and gastric cancer (GC) were included in the study. The resection materials and biopsy specimens were taken during the operation or endoscopy procedures. Identification of strains H. pylori, Candida spp and others was performed by established methods, on the basis of morphological, tinctorial, cultural and biochemical properties. Microflora of patients with different gastroduodenal diseases is diverse enough. It is represented by facultative, obligate anaerobes, microaeropilic bacteria. More frequently, there were H. pylori and Candida sp, as well as in associations and monocultures. The obtained results confirmed the wide distribution of H. pylori and Candida spp and their frequent coexistence in patients with gastric cancer, chronic gastritis and peptic ulcer disease. Microflora of patients with CG and GC was represented on 11 species. Microflora of patients with PUD-13 species was more diverse.展开更多
基金Supported by the University of Helsinki, the Helsinki University Central Hospital and the Finnish Cancer Organisations, Helsinki, Finland
文摘AIM: To compare the prevalence of Helicobacter pylori (Hpylori) IgG and IgA antibodies between adult subjects,with defined gastric diseases, nondefined gastric disorders and those representing the population.METHODS: Data on H pylori IgG and IgA antibodies,determined by enzyme immunoassay, were analyzed in 3 252 subjects with DGD including 482 patients with gastric ulcer, 882 patients with duodenal ulcer, 1 525patients with chronic gastritis only and 363 subjects with subsequent gastric cancer, 19 145 patients with NoDg and4 854 POPUL subjects. The age-adjusted prevalences were calculated for 1- and 20-year age cohorts.RESULTS: The prevalences of IgG antibodies were equally high (89-96%) in all 20-year age cohorts of the DGD groups, whereas the prevalences of IgG antibodies were lower and increased by age in the POPUL and NoDg groups. The prevalences of IgA antibodies were also higher in the DGD groups; among them CA (84-89%) and GU groups (78-91%) showed significantly higher prevalences than DU (68-77%) and CG patients (59-74%) (OR 2.49, 95%CI 1.86-3.34 between the GU and DU groups). In the CA, GU, and DU groups, the IgA prevalences showed only minor variation according to age, while they increased by age in the CG, POPUL, and NoDg groups (P≤0.0001). The IgA response, but not the IgG response, was associated with an increased risk of CA (OR 2.41, 95%CI 1.79-3.53) and GU (OR 2.57,95%CI 1.95-3.39) in comparison with CG patients.CONCLUSION: An IgA antibody response during H pylori infection is significantly more common in CA and GU patients as compared with CG patients.
文摘AIM： To investigate the role of gastric mucosa at the secretion of sTREM-1 in peptic ulcer.METHODS： Seventy two patients were enrolled; 35 with duodenal, 22 with gastric ulcer and 26 with chronic gastritis. Patients were endoscoped and gastric juice was aspirated. Patients with duodenal and gastric ulcer underwent a second endoscopy post-treatment. Biopsies were incubated in the absence/presence of endotoxins or gastric juice. Supernatants were collected and sTREM-2 and TNF~ were measured by enzyme immunoabsorbent assays. Scoring of gastritis was performed before and after treatment according to updated Sydney score.RESULTS： Patients with duodenal and gastric ulcer and those with chronic gastritis had similar scores of gastritis, sTREM-1 was higher in supernatants of tissue samples of H pylori-positive than of H pylori-negative patients with gastric ulcer. Median （± SE） sTREM-1 was found increased in supernatants of patients with gastric ulcer before treatment （203.21 ± 88.91 pg/1000 cells） compared to supernatants either from the same patients post-treatment （8.23 ± 5.79 pg/1000 cells） or from patients with chronic gastritis （6.21 ± 0.71 pg/1000 cells） （P 〈 0.001 and 〈 0.001, respectively）. Similar differences for sTREM-1 were recorded among LPS-stimulated tissue samples of patients （P = 0.001）. Similar differences were not found for TNFα. Positive correlations were found between sTREM-1 of supernatants from patients with both duodenal and gastric ulcer before treatment and the degree of infiltration of neutrophils and monocytes.CONCLUSION： sTREM-1 secreted by the gastric mucosa is an independent mechanism connected to the pathogenesis of peptic ulcer, sTREM-1 was released at the presence of H pylori from the inflamed gastric mucosa in the field of gastric ulcer.
文摘H. pylori infection is mainly spread in the kind of gastroduodenal diseases: chronic gastritis, peptic ulcer disease, MALT-lymphoma, gastric cancer. According to certain literature, the mentioned bacterium causes diseases of other visceral organs of humans. Study of the aggravating impact of this infection is under the attention of the scientists. However, other infectious agents, including fungi, other bacteria, parasites, and viruses and their role in different gastroduodenal diseases are not studied enough. The aim of our study was to identify mucous (parietal) gastroduodenal microflora in patients with different diseases of this zone. 390 patients with chronic gastritis (CG), peptic ulcer diseases (PUD) and gastric cancer (GC) were included in the study. The resection materials and biopsy specimens were taken during the operation or endoscopy procedures. Identification of strains H. pylori, Candida spp and others was performed by established methods, on the basis of morphological, tinctorial, cultural and biochemical properties. Microflora of patients with different gastroduodenal diseases is diverse enough. It is represented by facultative, obligate anaerobes, microaeropilic bacteria. More frequently, there were H. pylori and Candida sp, as well as in associations and monocultures. The obtained results confirmed the wide distribution of H. pylori and Candida spp and their frequent coexistence in patients with gastric cancer, chronic gastritis and peptic ulcer disease. Microflora of patients with CG and GC was represented on 11 species. Microflora of patients with PUD-13 species was more diverse.
