Biological pretreatment is a promising way to overcome the biorecalcitrance of cleaving the super- molecular structure of lignocellulose by lignin degrading enzymes from microorganisms. Solid state fermentation of cor...Biological pretreatment is a promising way to overcome the biorecalcitrance of cleaving the super- molecular structure of lignocellulose by lignin degrading enzymes from microorganisms. Solid state fermentation of corn stover with the white-rot fungus Phanerochaete chrysosporium was carried out and the efficiency of this pretreatment was evaluated. The enzymatic hydrolysis yield reached a maximum when the corn stover was biologically pretreated for nine days, and the hydrolysis yield decreased sharply if the solid state fermentation was carried out for more than nine days. A possible explanation for this sharp decrease is that not only the lignin degrading enzymes （LiP and MnP） were secreted, but also other metabolites, which were toxic or fatal to the hydrolysis enzymes resulting in the lower hydrolysis yield were generated during the prolonged period of biopretreatment. These results are usefuI to help determine the optimal timing and to understand the lignin structure and degradation mechanism in biological pretreatment processes.展开更多
基金Acknowledgements This research was supported by the State Key Development Program for Basic Research of China （Grant No. 2011CB707406）, the National Natural Science Foundation of China （Grant No. 20976051）, Ministry of Education of China （Grant No. 20090074110013）, the Shanghai Leading Academic Discipline Project （B505）, and the Fundamental Research Funds for the Central Universities of China （WF0913005）.
文摘Biological pretreatment is a promising way to overcome the biorecalcitrance of cleaving the super- molecular structure of lignocellulose by lignin degrading enzymes from microorganisms. Solid state fermentation of corn stover with the white-rot fungus Phanerochaete chrysosporium was carried out and the efficiency of this pretreatment was evaluated. The enzymatic hydrolysis yield reached a maximum when the corn stover was biologically pretreated for nine days, and the hydrolysis yield decreased sharply if the solid state fermentation was carried out for more than nine days. A possible explanation for this sharp decrease is that not only the lignin degrading enzymes （LiP and MnP） were secreted, but also other metabolites, which were toxic or fatal to the hydrolysis enzymes resulting in the lower hydrolysis yield were generated during the prolonged period of biopretreatment. These results are usefuI to help determine the optimal timing and to understand the lignin structure and degradation mechanism in biological pretreatment processes.
