Molecular mechanism of Mimenghua Granules in treating dry eye

Objective:To explore the molecular mechanism of Mimenghua Granules in treating dry eye based on network pharmacology and bioinformatics methods.Methods:Screening and prediction of possible blood-inducing active ingredients and action target of Mimenghua Granules through Traditional Chinese Medicine Systems Pharmacology database and analysis platform;mining dry eye-related diseases through disease gene database,gene target;use the functional protein combined network database STRING to draw the component-target and disease-target PPI networks,and extract the intersection of these two networks;use DAVID database analysis to screen key targets and analyze the mechanism of action.Results:A total of 593 active ingredients related to Mimenghua Granules were retrieved from the Traditional Chinese Medicine Systems Pharmacology database,and 59 blood active ingredients were obtained by screening based on pharmacokinetic parameters,and 680 targets related to these ingredients were retrieved;from disease genes,the database searches for 47 genes directly related to dry eye;3 key genes(ICAM1,IFNG,and IL-6)were obtained after the intersection of the component target and disease target PPI network;these genes are mainly involved in natural killer cell-mediated cytotoxicity,Jak-STAT signaling pathway,and Cytokine-cytokine receptor interaction.Conclusion:The mechanism of Mimenghua Granules in treating dry eye is related to the interaction of cytotoxic pathway,Jak-STAT signal pathway,and cytokines.The key gene targets are ICAM1,IFNG,and IL-6.

基于系统药理学菊花清热解毒功效的分子机制研究

基于系统药理学方法探索菊花(Flos chrysanthemi)清热解毒功效的分子机制,利用TCMSP、Drugbank、STRING、DAVID、BIOGPS等在线数据库,进行化合物筛选、网络构建、核心靶点筛选、信号通路富集与归经分析。结果表明,菊花主要通过槲皮素、山奈酚、木犀草素、柚皮素等21种化合物,作用于255个靶点发挥药效作用。JUN、TP53、AKT1、MAPK3、MAPK1、RELA、TNF等为菊花的核心靶点,通过GO功能富集分析得到146个GO条目,应用KEGG通路注释分析,菊花靶点主要作用在炎症和免疫相关信号通路。通过靶点归经分析,菊花核心靶点主要分布在肺脏、平滑肌与肝脏。菊花可能是通过槲皮素、山奈酚、木犀草素等核心化合物作用在肺脏、肝脏等器官发挥清热解毒作用,其机制与调控炎症相关信号通路通过AKT1、TNF等靶点发挥抗炎作用有关。

Fingerprinting Analysis of Four Variants of Chrysanthemi Morifoli Flos by RP-HPLC

Objective To establish a RP-HPLC fingerprinting analysis method for quality evaluation and control of the four variants of Chrysanthemi Morifoli Flos.Methods RP-HPLC was used to establish the fingerprinting method.Results Despite of the similarity in terms of holistic HPLC chromatograms,the four variants of Chrysanthemi Morifoli Flos exhibit characteristic fingerprints and can be readily recognized by similarity clusters.Conclusion A simple and reliable HPLC fingerprinting method has been developed and validated to authenticate the four variants of Chrysanthemi Morifoli Flos,providing a scientific basis for quality control of Chrysanthemi Morifoli Flos.

Anti-diabetic effects of linarin from Chrysanthemi Indici Flos via AMPK activation

Objective:The purpose of this study is to investigate the anti-diabetic effects of linarin,a flavonoid extracted from Chrysanthemi Indici Flos(CIF),and its potential mechanisms.Methods:The effects of linarin on cell viability and glucose consumption in Hep G2 cells were measured.Meanwhile,monosodium glutamate(MSG) mouse model was constructed to monitor the changes of insulin tolerance,glucose tolerance,triglyceride and cholesterol.The protein expression levels of pAMPK,p-ACC,PEPCK and p-GS were detected by Western blot.Results:Linarin could increase the relative glucose consumption of Hep G2 cells,improve insulin tolerance and glucose tolerance,and decrease the levels of triglyceride and cholesterol of MSG mice.Simultaneously,the expression levels of p-AMPK and p-ACC in Hep G2 cells and the liver tissue of MSG mice were increased,while the expression levels of PEPCK and p-GS were decreased after treatment with linarin.Conclusion:Insulin resistance could be ameliorated by linarin in type 2 diabetes,and its mechanism may be related to AMPK signaling pathway.