Objective: To investigate the antioxidant and ɑ-glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum(Desf.)(C. trifu...Objective: To investigate the antioxidant and ɑ-glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum(Desf.)(C. trifurcatum). Methods: For extraction from roots, stems, leaves and flowers of C. trifurcatum, methanol, ethyl acetate and petroleum ether solvents were used. Phenols, flavonoids, flavonols and tannins contents were evaluated. More, C. trifurcatum aerial parts oil composition was determined using chromatography/mass spectrometry. The antioxidant effect was estimated by DPPH, ABTS and reducing power test systems. The ɑ-glucosidase inhibition was determined by colorimetric assay using the enzyme from Aspergillus niger and the p-nitrophenyl glucopyranoside(pNPG) as substrate. Results: The highest amounts of polyphenols, flavonoids, flavonols and tannins were shown by the methanolic extract of leaves. The main components of the aerial parts oil were limonene(29.21%), γ-terpinene(12.96%), 4-terpenyl acetate(12.18%) and ɑ-pinene(5.76%). The activity evaluated by DPPH, ABTS and reducing power tests was important for stems(IC_(50)=0.68 mg/mL) and flowers(IC_(50)=0.67 mg/mL) methanolic extracts and essential oil(IC_(50)=0.72 mg/mL). Findings of ɑ-glucosidase activity revealed that petroleum ether extracts of leaves and roots together with aerial parts oil showed a highest activity with IC_(50) of 0.044, 0.045 and 0.049 mg/m L, respectively. Conclusions: Observed antioxidant and ɑ-glucosidase activities of oil and extracts are attributed to the presence of the active phytochemicals in C. trifurcatum organs. Thus, the C. trifurcatum can be used as a source of antioxidant compounds and dietary supplement to treat patients with type 2 diabetes.展开更多
文摘Objective: To investigate the antioxidant and ɑ-glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum(Desf.)(C. trifurcatum). Methods: For extraction from roots, stems, leaves and flowers of C. trifurcatum, methanol, ethyl acetate and petroleum ether solvents were used. Phenols, flavonoids, flavonols and tannins contents were evaluated. More, C. trifurcatum aerial parts oil composition was determined using chromatography/mass spectrometry. The antioxidant effect was estimated by DPPH, ABTS and reducing power test systems. The ɑ-glucosidase inhibition was determined by colorimetric assay using the enzyme from Aspergillus niger and the p-nitrophenyl glucopyranoside(pNPG) as substrate. Results: The highest amounts of polyphenols, flavonoids, flavonols and tannins were shown by the methanolic extract of leaves. The main components of the aerial parts oil were limonene(29.21%), γ-terpinene(12.96%), 4-terpenyl acetate(12.18%) and ɑ-pinene(5.76%). The activity evaluated by DPPH, ABTS and reducing power tests was important for stems(IC_(50)=0.68 mg/mL) and flowers(IC_(50)=0.67 mg/mL) methanolic extracts and essential oil(IC_(50)=0.72 mg/mL). Findings of ɑ-glucosidase activity revealed that petroleum ether extracts of leaves and roots together with aerial parts oil showed a highest activity with IC_(50) of 0.044, 0.045 and 0.049 mg/m L, respectively. Conclusions: Observed antioxidant and ɑ-glucosidase activities of oil and extracts are attributed to the presence of the active phytochemicals in C. trifurcatum organs. Thus, the C. trifurcatum can be used as a source of antioxidant compounds and dietary supplement to treat patients with type 2 diabetes.
