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高效液相色谱法同时测定豆制品中的碱性橙和碱性嫩黄O染料 被引量:51
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作者 林钦 《色谱》 CAS CSCD 北大核心 2007年第5期776-777,共2页
采用高效液相色谱法(HPLC)同时测定了豆制品中的碱性橙和碱性嫩黄O染料的含量,样品用乙腈提取后用正己烷脱脂净化,然后在碱性条件下用乙醚萃取,最后溶于甲醇中进行HPLC分离、检测。所使用的色谱柱为GeminiC18(5μm,4.6mm×150mm),... 采用高效液相色谱法(HPLC)同时测定了豆制品中的碱性橙和碱性嫩黄O染料的含量,样品用乙腈提取后用正己烷脱脂净化,然后在碱性条件下用乙醚萃取,最后溶于甲醇中进行HPLC分离、检测。所使用的色谱柱为GeminiC18(5μm,4.6mm×150mm),紫外检测波长分别为458nm(碱性橙)和436nm(碱性嫩黄O),流动相为0.03%磷酸溶液/甲醇,梯度洗脱。碱性橙的检出限为0.01mg/kg,碱性嫩黄O的检出限为0.02mg/kg。回收率为71%~80%,相对标准偏差(RSD)为2.2%~5.0%(n=6),两种染料在0.2~4.0μg/mL内呈良好的线性关系。 展开更多
关键词 高效液相色谱法(HPLC) 豆制品(beanproducts) 碱性橙(chrysoidine) 碱性嫩黄O(auramine O)
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Rapid determination of illegal additives chrysoidin and malachite green by surface-enhanced Raman scattering with silanized support based substrate
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作者 Han Zhang Lin Sun +4 位作者 Yuan Zhang Yan Kang Huilian Hu Jibran Iqbal Yiping Du 《Chinese Chemical Letters》 SCIE CAS CSCD 2018年第6期981-984,共4页
This paper reports accurate use of silanized support based substrate for surface-enhanced Raman spectroscopy(SERS) determination of illegal additives chrysoidin and malachite green. Detection of these illegal additi... This paper reports accurate use of silanized support based substrate for surface-enhanced Raman spectroscopy(SERS) determination of illegal additives chrysoidin and malachite green. Detection of these illegal additives is very meaningful and the developed method meets this objective in a simple, rapid and cost effective way. Detection limits for chrysoidin and malachite green in aqueous solutions reached0.001 mg/L and 0.0001 mg/L respectively. Linear relationship between the Raman intensities and chrysoidin concentrations in range of 0.001-0.05 mg/L was found with regression coefficient(R) of 0.994.To investigate the practical application performance of the method, the substrate was applied to detect chrysoidin in Sprite at 0.01 mg/L and malachite green in fish pond water at 0.0001 mg/L. The SERS method could be an alternative approach for monitoring illegal additives and toxic harmful substances. 展开更多
关键词 Surface-enhanced Raman spectroscopy(SERS) Silanized support Chrysoidin Malachite green Illegal additives
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