Interaction between seed size and NaCl on germination and early seedling growth of some Turkish cultivars of chickpea(Cicer arietinum L.)

Chickpea is an important food legume crop of Turkey and is largely grown for human consumption on low moisture or salt-affected soils. The objective of the study was to find the effects of NaCl stress at electrical conductivities of 4.5,8.6,12.7 and 16.3 dS/m and seed sizes(7,8 and 9 mm) on germination and early seedling growth of three popular chickpea cultivars(AKN-97,Gokce and Uzunlu-99). Mean frequency of germination,germination time,germination index,root length,shoot length and seedling fresh weight showed seed size-dependent responses of cultivars to salt stress. In general,small seeds germinated and grew more rapidly compared to medium and large seeds of the same cultivars against all levels of salt stress,with the best results in cultivar Uzunlu-99. No effect of NaCl treatments was observed on frequency of germination;however,a drastic decrease in early seedling growth was recorded at increased NaCl concentrations. Regression analysis results showed a significantly positive rela-tionship(P<0.01) between seed size and mean germination time,whereas a significantly negative relationship was recorded between seed size and germination index,root length,shoot length. Moreover,linear regression values apparently confirmed that increased seed size in each cultivar affected decreased germination index,root and shoot lengths with enhanced mean germination time. Thus,it was concluded that the use of small seeds could considerably reduce the production costs of chickpea in salt-affected soils.

Effect of Cultivar, Irrigation and Nitrogen Fertilization on Chickpea (<i>Cicer arietinum</i>L.) Productivity

A 2-year field study was conducted in northern Greece to investigate the effect of nitrogen fertilization and irrigation on productivity of three Greek chickpea varieties (“Amorgos” “Serifos”, “Andros”). Chickpea, grown under irrigation regime (30 + 30 mm of water) and fertilized with 50 kg·N·ha-1 before planting and with 40 kg·N·ha-1 at blossom growth stage, produced more total dry biomass and seed yield as compared with that grown under non-irrigated conditions and fertilized with 50 kg·N·ha-1 before planting only. In particular, irrigation and nitrogen fertilization at blossom growth stage increased total dry weight of chickpea by 18.3% and 18.5%, respectively, as compared with that of non-irrigated and fertilized with N before planting. The corresponding increase of seed yield was 30.5% and 20%, respectively. The total dry biomass of “Amorgos” was 10% and 13% greater than that of “Serifos” and “Andros”, while its respective seed yield increase was 5% and 16%. Finally, the quantum yield of photosystem II of chickpea was not affected by irrigation or fertilization. These results indicated that nitrogen fertilization at blossom growth stage combined with irrigation increased seed yield of all chickpea varieties, whereas the same treatments did not have any effect on plant quantum yield of photosystem II.

Rhizogenesis of Two Species Fabaceae: <i>Cicer arietinum</i>L. and <i>Pisum sativum</i>L.

Our work of comparative study on the biomorphological, rhizogenic level of Chickpea (Cicer arietinum) and Pea (Pisum sativum), includes successively the following results: The in-vitro culture done, showed strains, which can reach 6.5 mm in the medium Nutrient agar (NA) for Cicer arietinum 6 mm of strain were recorded in the PDA culture medium for Pisum sativum. The best strain is obtained in temperature of 20&#8451;(ambient). According to the results of the rhizogenesis, we notice that the nutrient agar (NA) medium responds better that the PDA medium for Cicer arietinum. Meanwhile, the medium PDA brings a better reaction (response) compared with the nutrient agar medium concerning Pisum sativum.

Yield and Water Productivity of Chickpea (<i>Cicer arietinum</i>L.) as Influenced by Different Irrigation Regimes and Varieties under Semi Desert Climatic Conditions of Sudan

A field experiment was conducted at Hudeiba Research Station Farm, located at Ed-Damer, Sudan during 2011/2012 and 2012/2013 winter seasons to investigate the effect of different irrigation regimes and varieties on chickpea (Cicer arietinum L.) yield, yield components and water productivity. The treatments include three irrigation regimes;irrigation every 10 days (I1 = full irrigation), irrigation every 15 days (I2 = moderate stress) and irrigation every 20 days (I3 = severe stress) and two varieties (Borgieg and Wad Hamid). The treatments were arranged in factorial randomized complete block design (RCBD) with 3 replications. Irrigation water being applied, grain yield, yield components (number of pods per plant, number of seeds per pod and the 100 seeds weight) and crop water productivity (CWP) and irrigation water productivity (IWP) were recorded. Results showed that the number of pods per plant, number of seeds per pod, 100-seeds weight, grain yield and irrigation water applied were significantly (p ≤ 0.001) affected by irrigation regimes. The highest values of these traits obtained with full irrigation, whereas the lowest values were recorded under severe water stress conditions. Results also indicated that, moderate and severe water stress regimes saved irrigation water by 24% and 32%, respectively compared with full irrigation. This study indicated that treatment I1 which was irrigated every 10-days did not produce the highest IWP, while treatment I2 which irrigated every 15-days gave the highest IWP. The lowest IWP occurred at severe water stress regime (I3). It could be concluded that moderate water stress might be adopted. Contrarily, the adoption of severe water stressed that produce high water savings would lead to yield losses that might be economically not acceptable. The late maturing chickpea variety of Borgieg significantly (p ≤ 0.05) out-yielded the early maturing variety Wad Hamid by 11%. Borgieg displayed the highest values of CWP and IWP.

A Kunitz trypsin inhibitor from chickpea (<i>Cicer arietinum</i>L.) that exerts an antimicrobial effect on Fusarium oxysporum f.sp. ciceris

Fusarium oxysporum f.sp. ciceris (Foc) is one of the most important fungal pathogens of chickpea and is regarded as a constant threat in tropical and subtropical countries. In order to correlate Fusarium wilt resistance/susceptibility in Cicer arietinum to the presence or absence of trypsin inhibitor (TI) in the crude extract, trypsin inhibitory assay (TIA) and in vitro activity of TI against Foc were studied. In the present study, a 20 kDa trypsin inhibitor was purified from Fusarium wilt resistant cultivar (viz. JG 2001-12) by ammonium sulfate precipitation, dialysis and chromatographies with Sephadex G-100 and Diethyl aminoethyl cellulose (DEAE-cellulose-52) ion-exchange column. Results of pathogenecity assay were found to be in correlation to the trypsin inhibitor assay where the Fusarium wilt resistant cultivar showed high trypsin inhibitory activity (99%) in the presence of trypsin enzyme using both natural and synthetic substrates. Preliminary studies using crude extracts of JG 2001-12 showed a decrease in radial growth of Foc. A 45%-82% reduction in conidium germination at 20 μg&middotmL-1?Cicer arietinum trypsin inhibitor (CaTI) concentration was observed, thereby, indicating the use of CaTI in suppression of pathogen and in its deployment through transgenic plants for the management of Fusarium wilt.

Variation in Thermal time model Parameters Between Two Contrasting Chickpea (<i>Cicer arietinum</i>) cultivars

A laboratory experiment was carried out to determine the effect of different constant temperatures on germination and early seedling establishment and to study the variation among parameters of thermal time model parameters for two contrasting chickpea cultivars . Seeds were subjected to six constant temperatures from 10 o C to 35 o C . A complete randomized design was used with four replication. Analysis of variance showed significant differences among treatments for all characters studied. The final germination percentage significantly increased with increasing temperature up to 25 ° C, and thereafter there was a sharp decrease in final germination at 30 ° and 35 ° C. Desi type cultivar (small seeded) “Jabel Marra” significantly exhibited higher final germination percentage and lower germination rate compared with the kabui type cultivar “Shendi” at all temperatures. The median (θ T(50) ) of the thermal time was significantly differ between the two chickpea cultivars. The large seeded cultivars (shendi) recorded significantly higher median thermal time than the small seeded cultivars (Jabel Marra). The results also revealed a significant differences between the two cultivars in all thermal time model parameters. The small seeded cultivar (Jabel Marra) scored lower total dry matter and temperature tolerance index (TTI) compared to the large seeded cultivar (Shendi) at all temperatures studied.

鹰嘴豆(Cicer arietinum)TCP基因家族的生物信息学分析

TCP蛋白是植物特有转录因子家族之一,参与植物的生长发育调节过程。本研究利用生物信息学方法,通过PlantTFDB database、NCBI、CLUSTALX 2.0、GSDS 2.0、MEME和psRNATarget数据库以及MEGA 5.0等软件,对鹰嘴豆TCP基因家族成员进行分子量、等电点、染色体定位、基因保守域、保守基序、microRNA的靶位点和系统发育进化树的分析。研究表明:TCP基因可被分为ClassⅠ(PCF)和ClassⅡ(CYC/TB1和CIN)两大类,在染色体上的分布较为均匀,具有较保守的螺旋-环-螺旋结构域(Helix loop helix domain,bHLH),含有3个Motif序列,其中每个家族成员共有的是Motif2。microRNA靶位点预测发现,TCP基因的Ⅱ类CIN亚家族中的CarTCP2和CarTCP23受miR319调控。以上结果简要概述了鹰嘴豆TCP基因家族的进化、基因结构及蛋白的生化特性,旨在为今后更好地揭示该基因在鹰嘴豆的生长发育和生理特性中的功能提供理论基础。

Gene Expression Profiling during Wilting in Chickpea Caused by <i>Fusarium oxysporum</i>F. sp. <i>Ciceri</i>

Fusarium oxysporum f. sp. ciceri (Foc), one of the most important fungal pathogen of chickpea, is a constant threat to this crop plant. In the present study gene expression analysis of chickpea roots during Foc infection was performed using various approaches. cDNAs derived from total mRNA during infection process of susceptible (JG62)and resistant (Digvijay) cultivars, were amplified using random oligonucleotides. Sequence characterization of differentially expressed transcripts revealed their homology with many plant genes essential for various metabolic functions including defense. Further, expression patterns of specific candidate gene transcripts were analyzed in the Foc inoculated and uninoculated resistant and susceptible chickpea cultivars, on day 6 of infection. Semiquantitative RT-PCR analysis of defense related genes was performed using gene specific oligonucleotides in resistant and susceptible chickpea cultivars. The expression of fungal pathogenesis related genes and their race specific response was determined throughout the course of chickpea-Foc interaction. Temporal expression and race specific response of plant defense related and fungal virulence genes were studied in the resistant and susceptible cultivars of chickpea inoculated with three races of Foc highlighting the host-pathogen interactions. Few genes, involved in chickpea defense against Fusarium wilt which were not reported previously were unveiled in this study.

人工老化对鹰嘴豆种子发芽和膜透性的影响

以鹰嘴豆种子为研究材料,分别进行12 h、24 h、36 h、48 h、60 h、72 h、84 h及96 h 8个人工老化处理(40℃、相对湿度100%),研究老化过程中种子的发芽率、发芽指数、活力指数、苗长、根长、电导率及可溶性糖的变化趋势,分析人工老化处理对鹰嘴豆种子膜透性的影响。结果表明,鹰嘴豆种子在老化处理后,发芽率、发芽指数先急剧下降(12 h)而后缓慢下降,浸种液电导率先缓慢上升而后急剧上升(48 h),而可溶性糖含量先急剧上升(12 h)后缓慢上升再略有下降(96 h)。不同老化程度种子的电导率与发芽指数和发芽率均呈显著正相关(P<0.05),发芽率与发芽指数呈显著正相关(P<0.05),可溶性糖与活力指数呈极显著正相关(P<0.01),其他各指标间无显著相关性。

响应面结合遗传算法优化鹰嘴豆中抗炎活性成分的提取

以异黄酮提取率为评价指标,考察超声时间、乙醇浓度和料液比对鹰嘴豆异黄酮提取率的影响,在单因素实验基础上,利用响应面结合遗传算法优化鹰嘴豆异黄酮提取工艺,得到最佳提取工艺条件为:提取时间为1.73 h、乙醇浓度为78.64%,料液比1∶24.9 g/mL,鹰嘴豆中异黄酮提取率为0.76 mg/g。以5-脂氧合酶为作用靶点,利用超滤-质谱技术(UF-MS)筛选鹰嘴豆中具有抑制5-脂氧合酶作用的潜在抗炎活性成分。结果表明,利用响应面结合遗传算法可以进一步优化提高鹰嘴豆中活性成分的提取率;鹰嘴豆中具有潜在的抗炎活性成分为大豆苷、大豆苷元、毛蕊异黄酮、后莫紫檀素和鹰嘴豆芽素A,其结合强度分别为40.82%、36.93%、31.65%、28.75%和22.51%。

鹰嘴豆种质资源农艺性状遗传多样性分析

以100份鹰嘴豆种质资源为材料,应用聚类分析和主成分分析方法,对15个主要农艺性状的遗传多样性进行分析。结果表明,参试材料存在广泛的遗传多样性。其中,多样性指数最高的是株高,其次是百粒重;性状变异系数最大的是单株荚数,其次是单株粒重;基于各种质间形态标记的遗传差异,将100份鹰嘴豆种质聚类并划分为4大类群。第Ⅰ类群可作为选育丰产中粒型和株高适中的品种,第Ⅱ类群可作为选育矮秆耐密及特异粒色(型)品种,第Ⅲ类群丰产性较差可作为选育子粒球型、光滑的品种,第Ⅳ类群可作为选育大粒型、适宜机械化收获的品种。9个数量性状的主成分分析结果表明,前4个主成分累计贡献率达73.91%,各主成分性状载荷值反映了主要数量性状的育种选择潜力。综合分析种质资源农艺性状,为鹰嘴豆的有效利用提供一定的科学依据。

鹰咀豆根系分泌物的分离鉴定及典型分泌物苯甲醛的化感效应

运用CH2Cl2提取水培35d鹰咀豆的根系分泌物,并通过气相色谱和质谱联用技术(GC MS)进行分离鉴定,对典型分泌物苯甲醛的生物学效应通过发芽试验进行了测试。结果表明,鹰咀豆的根分泌物主要包括有机酸、酚、烃类、酯、酮类、醇、酰胺、苯、醛和噻唑等;苯甲醛是一种他感作用强于自毒作用的化感物质,对2种幼苗组成的复合系统的负效应小于对单作系统的负效应。

富钒鹰嘴豆芽对糖尿病大鼠血糖和血脂的影响

目的:研究富钒鹰嘴豆芽降糖作用及可能机制。方法:以链脲佐菌素诱导形成糖尿病大鼠模型,饲喂不同浓度的富钒鹰嘴豆芽4周,观察大鼠血糖和血脂等的变化。结果:与正常对照组比较,糖尿病模型大鼠的血糖升高了4倍,血浆胰岛素降低了57.63%、糖化血红蛋白(GHb)升高了42.28%,谷丙转氨酶、谷草转氨酶和血浆脂质水平明显升高。而分别饲喂不同浓度富钒鹰嘴豆芽4周后,糖尿病大鼠的上述指标都得到改善,其中以中等剂量(100μg/ml)富钒鹰嘴豆芽治疗组的效果最明显:血糖水平降低了58.60%(P<0.05);血浆胰岛素水平明显增高(P<0.05)且接近正常;GHb降低了32.81%,血浆低密度脂蛋白、甘油三酯和总胆固醇明显下降(P<0.05),谷丙转氨酶和谷草转氨酶分别降低了39.98%和41.37%。而葡萄糖耐量得到显著改善,2h的血糖值降低了38.46%。结论:富钒鹰嘴豆芽能阻止糖尿病大鼠高血糖的进展和血浆脂质水平的改变;这种钒的有机复合物形式达到了减毒增效的目的,具有较佳的应用前景。

鹰嘴豆种子胰蛋白酶抑制剂的分离纯化与鉴定

为了寻找具有药物作用的天然胰蛋白酶抑制物,采用硫酸铵分级沉淀、离子交换层析(DEAE-纤维素52)及Sephadex G-100凝胶层析等方法,从鹰嘴豆种子中分离出一种鹰嘴豆胰蛋白酶抑制剂(CPTI).研究表明:CPTI对胰蛋白酶有较强的抑制作用,抑制率达80%,而对胰凝乳蛋白酶抑制作用较弱,抑制率为32%,对胃蛋白酶、木瓜蛋白酶及枯草杆菌蛋白酶均无抑制作用;用SDS-PAGE测得CPTI近似分子质量为25.7 kD;CPTI具有较高的热稳定性,在100℃下加热60min,对胰蛋白酶活性仍保持78%抑制率;Lineveaer-Burk作图得知该抑制剂属竞争性抑制类型.动力学测定显示,来自鹰嘴豆中的CPTI对胰蛋白酶的抑制作用常数(Ki)为3.99×10-7 mol/L.

GC-MS应用于鹰嘴豆中弱极性化学成分的研究(英文)

应用气相色谱-质谱分析技术,首次对鹰嘴豆中弱极性成分进行研究。从鹰嘴豆弱极性萃取部位中鉴定了22个化合物,以脂肪酸为主要成分。脂肪酸含量(质量分数)高达81.25%,其中9-十八烯酸和8,11-十八碳二烯酸的含量分别占总量的28.00%和38.60%。

鹰嘴豆化学成分研究

目的:研究鹰嘴豆的化学成分,从中寻找具有生物活性的天然化合物。方法:采用不同体积分数的乙醇对鹰嘴豆干燥种子及鹰嘴豆发芽种子进行提取,经硅胶、大孔树脂D101、葡聚糖Sephadex LH-20色谱手段分离纯化,通过NMR,MS等波谱分析手段鉴定化合物结构。结果:分离到9个化合物,分别鉴定为3-羟基-齐墩果-12-烯(1),鹰嘴豆芽素A-7-O-β-D-葡萄糖苷(2),脑苷(3),1-乙基-α-L-半乳糖苷(4),尿苷(5),腺苷(6),色氨酸(7),鹰嘴豆芽素A(8),芒柄花素(9)。结论:1,3,4,6,7均为首次从鹰嘴豆属植物中分离到。

鹰嘴豆总皂苷对2型糖尿病大鼠抗氧化作用的影响

目的观察鹰嘴豆总皂苷(TSCA)对2型糖尿病大鼠抗氧化作用的影响。方法以高脂饲料喂养后的雄性Wistar大鼠腹腔注射链脲佐菌素(STZ)诱导2型糖尿病模型,其中两组造模动物分别灌胃给予鹰嘴豆总皂苷100 mg/kg(低剂量组),300 mg/kg(高剂量组)。4周后,测定各组大鼠肝脏、肾脏、肺、骨骼肌等组织中相关抗氧化指标。结果与模型组比较,鹰嘴豆总皂苷低剂量组能够显著升高肾脏中总超氧化物岐化酶(TSOD)水平(P<0.01)、肝脏、肾脏、骨骼肌中总一氧化氮合酶(TNOS)活力(P<0.01)和肺、骨骼肌中谷胱甘肽过氧化物酶(GSH-PX)活性(P<0.01),且能升高骨骼肌中总超氧化物岐化酶(TSOD)水平及过氧化氢酶(CAT)活力(P<0.05),显著降低肝脏、骨骼肌中丙二醛(MDA)的含量(P<0.01);鹰嘴豆总皂苷高剂量组能够显著升高肾脏总超氧化物岐化酶(TSOD)水平(P<0.01)和肝脏、肾脏、骨骼肌中TNOS活力(P<0.01),且能升高骨骼肌总超氧化物岐化酶(TSOD)水平、谷胱甘肽过氧化物酶(GSH-PX)活性、过氧化氢酶(CAT)活力、肾脏过氧化氢酶(CAT)活力(P<0.05),显著降低肝脏MDA的含量(P<0.01)。结论此研究提示鹰嘴豆总皂苷可以改善2型糖尿病大鼠的抗氧化能力。

高活性大豆异黄酮糖苷酶的分离原料筛选、纯化及性质研究

通过对32种植物β-葡萄糖苷酶水解染料木苷的活性比较,发现鹰嘴豆β-葡萄糖苷酶具有4.19U/mg的大豆异黄酮糖苷水解酶活性。该酶经硫酸铵分级沉淀、DEAE-Cellulose-52离子交换、Sephadex G-100凝胶层析纯化,纯化了10.1倍,收率为7.2%;SDS-PAGE和Sephadex G-100凝胶层析结果表明,该酶的分子量为73.2kD,含两个亚基;该酶的最适反应温度为45℃;最适pH6.0;当以染料木苷和大豆苷为底物时该酶的米氏常数分别为11.0和19.0μg/ml。温度在30～45℃、pH值在4.5～6.5范围内该酶较稳定:Ag^+、Hg^(2+)和D-葡萄糖酸内酯对该酶有强烈抑制作用。该酶能水解染料木苷和大豆苷,但不能水解纤维二糖和α-乳糖。