Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and...Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and its effects on HBV intrauterine transmission. Methods We enrolled 290 newborns and their hepatitis B surface antigen(HBsAg) positive mothers. HBV cccDNA in PBMC and HBV DNA in serum were detected by a real‐time PCR‐TaqM an probe while HBV serological markers were detected with an electrochemiluminescence immunoassay. Results There was a positive correlation between the levels of PBMC HBV cccD NA and serum HBV DNA and HBeA g(r = 0.436 and 0.403, P < 0.001). The detection rate of pattern A [‘HBsA g(+), HBeA g(+), and anti‐HBc(+)’] was significantly higher in the PBMC HBV cccD NA positive group than in the control group(χ^2 = 48.48, P < 0.001). There was a significant association between HBV intrauterine transmission and PBMC HBV cccD NA(χ^2 = 9.28, P = 0.002). In the presence of serum HBV DNA, HBeA g, and PBMC HBV cccD NA, the risk of HBV intrauterine transmission was three times higher(OR = 3.69, 95% CI: 1.30‐10.42) than that observed in their absence. The risk of HBV intrauterine transmission was the greatest(OR = 5.89, 95% CI: 2.35‐14.72) when both PBMC HBV cccD NA and pattern A were present. A Bayesian network model showed that maternal PBMC HBV cccD NA was directly related to HBV intrauterine transmission. Conclusion PBMC HBV cccDNA may be a direct risk factor for HBV intrauterine transmission. Our study suggests that serological markers could be combined with PBMC‐related markers in prenatal testing.展开更多
针对现有的直升机桨叶欠曝光图像中圆形标记点检测方法存在自适应能力不强、速度慢、精度不高的问题,提出了基于YOLOv3(you only look once)与分水岭的直升机桨叶欠曝光图像圆形标记点检测方法.首先,将采集的真实桨叶欠曝光图像中的圆...针对现有的直升机桨叶欠曝光图像中圆形标记点检测方法存在自适应能力不强、速度慢、精度不高的问题,提出了基于YOLOv3(you only look once)与分水岭的直升机桨叶欠曝光图像圆形标记点检测方法.首先,将采集的真实桨叶欠曝光图像中的圆形标记点进行标注后,制作成数据集,并训练YOLOv3网络;其次,用训练好的YOLOv3网络检测出圆形标记点区域;再次,改进传统分水岭标记提取方式,采用多线程技术并行在各圆形标记点区域内进行分水岭变换,得到圆形标记点边缘检测结果;最后,采用最小二乘圆拟合和奇异点去除法实现圆形标记点的精确定位.研究者通过对多幅欠曝光桨叶图像中圆形标记点进行检测实验,验证了该方法具有自适应能力强、速度快、精度高的优点,并已将其用于直升机桨叶欠曝光图像圆形标记点的检测.展开更多
基金supported by grants from the National Natural Science Foundation of China [81573212,81872677]Open Project Support by the State Key Laboratory of Infectious Disease Prevention and Control [2017SKLID306,2018SKLID310]
文摘Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and its effects on HBV intrauterine transmission. Methods We enrolled 290 newborns and their hepatitis B surface antigen(HBsAg) positive mothers. HBV cccDNA in PBMC and HBV DNA in serum were detected by a real‐time PCR‐TaqM an probe while HBV serological markers were detected with an electrochemiluminescence immunoassay. Results There was a positive correlation between the levels of PBMC HBV cccD NA and serum HBV DNA and HBeA g(r = 0.436 and 0.403, P < 0.001). The detection rate of pattern A [‘HBsA g(+), HBeA g(+), and anti‐HBc(+)’] was significantly higher in the PBMC HBV cccD NA positive group than in the control group(χ^2 = 48.48, P < 0.001). There was a significant association between HBV intrauterine transmission and PBMC HBV cccD NA(χ^2 = 9.28, P = 0.002). In the presence of serum HBV DNA, HBeA g, and PBMC HBV cccD NA, the risk of HBV intrauterine transmission was three times higher(OR = 3.69, 95% CI: 1.30‐10.42) than that observed in their absence. The risk of HBV intrauterine transmission was the greatest(OR = 5.89, 95% CI: 2.35‐14.72) when both PBMC HBV cccD NA and pattern A were present. A Bayesian network model showed that maternal PBMC HBV cccD NA was directly related to HBV intrauterine transmission. Conclusion PBMC HBV cccDNA may be a direct risk factor for HBV intrauterine transmission. Our study suggests that serological markers could be combined with PBMC‐related markers in prenatal testing.
文摘针对现有的直升机桨叶欠曝光图像中圆形标记点检测方法存在自适应能力不强、速度慢、精度不高的问题,提出了基于YOLOv3(you only look once)与分水岭的直升机桨叶欠曝光图像圆形标记点检测方法.首先,将采集的真实桨叶欠曝光图像中的圆形标记点进行标注后,制作成数据集,并训练YOLOv3网络;其次,用训练好的YOLOv3网络检测出圆形标记点区域;再次,改进传统分水岭标记提取方式,采用多线程技术并行在各圆形标记点区域内进行分水岭变换,得到圆形标记点边缘检测结果;最后,采用最小二乘圆拟合和奇异点去除法实现圆形标记点的精确定位.研究者通过对多幅欠曝光桨叶图像中圆形标记点进行检测实验,验证了该方法具有自适应能力强、速度快、精度高的优点,并已将其用于直升机桨叶欠曝光图像圆形标记点的检测.
