Objective: To investigate the correlation of annexin A2 expression with epithelial-mesenchymal transition (EMT) and extracellular matrix (ECM) degradation in bile duct carcinoma. Methods: A total of 60 patients with p...Objective: To investigate the correlation of annexin A2 expression with epithelial-mesenchymal transition (EMT) and extracellular matrix (ECM) degradation in bile duct carcinoma. Methods: A total of 60 patients with primary bile duct carcinoma who accepted bile duct carcinoma laparotomy in our hospital between April 2009 and May 2017 were selected. The bile duct carcinoma tissue and adjacent normal tissue were kept during operation and enrolled in bile duct cancer group (n=60) and normal control group (n=60). The protein expression of annexin A2, EMT marker molecules, MMPs subtypes and TIMPs subtypes in two groups of specimens were detected, and Pearson test was used to evaluate the correlation of annexin A2 expression with EMT and ECM degradation in bile duct carcinoma tissue. Results: Annexin A2 expression in bile duct cancer group was higher than that in normal control group;E-cadherin, β-catenin and syndecan-1 protein expression in bile duct cancer group were lower than those in control group whereas P-cadherin and Vimentin protein expression were higher than those in normal control group;MMP-2, MMP-7 and MMP-9 protein expression in bile duct cancer group were higher than those in normal control group whereas TIMP-1 and TIMP-2 protein expression were lower than those in normal control group. Pearson test showed that the annexin A2 expression in bile duct carcinoma was directly correlated with the protein expression of EMT marker molecules, MMPs subtypes and TIMPs subtypes. Conclusion: annexin A2 increases in bile duct carcinoma, and it can regulate EMT and ECM degradation process to affect the disease outcome.展开更多
Pancreatic cancer continues to be a leading cause of cancer-related death worldwide and there is an urgent need to develop novel diagnostic and therapeutic strategies to reduce the mortality of patients with this dise...Pancreatic cancer continues to be a leading cause of cancer-related death worldwide and there is an urgent need to develop novel diagnostic and therapeutic strategies to reduce the mortality of patients with this disease. In pancreatic cancer, some tight junction proteins, including claudins, are abnormally regulated and therefore are promising molecular targets for diagnosis, prognosis and therapy. Claudin-4 and-18 are overexpressed in human pancreatic cancer and its precursor lesions. Claudin-4 is a high affinity receptor of Clostridium perfringens enterotoxin(CPE). The cytotoxic effects of CPE and monoclonal antibodies against claudin-4 are useful as novel therapeutic tools for pancreatic cancer. Claudin-18 could be a putative marker and therapeutic target with prognostic implications for patients with pancreatic cancer. Claudin-1,-7, tricellulin and marvelD3 are involved in epithelial to mesenchymal transition(EMT) of pancreatic cancer cells and thus might be useful as biomarkers during disease. Protein kinase C is closely related to EMT of pancreatic cancer and regulates tight junctions of normal human pancreatic duct epithelial cells and the cancer cells. This review focuses on the regulation of tight junctions via protein kinase C during EMT in human pancreatic cancer for the purpose of developing new diagnostic and therapeutic modalities for pancreatic cancer.展开更多
To analyze the existing schemes of high-speed rotorcrafts and some new technologies, a new conceptual sketch of the high-speed rotor/wing transition helicopter RD15 is proposed. The overall layout of the RD15 is given...To analyze the existing schemes of high-speed rotorcrafts and some new technologies, a new conceptual sketch of the high-speed rotor/wing transition helicopter RD15 is proposed. The overall layout of the RD15 is given out and the transition process from the helicopter mode to the airplane mode is designed. The lift system consists of a circular disk-wing with four retractable blades. The technology of individual blade control is adopted for flight control in hover and low speed flight. The tail is a vectored thrust duct propeller. It can provide the anti-torque in hover, and offer the multi-directional controls and propulsion drive for the airplane mode flight. The aerodynamic characteristics and key technologies in the transition process for this layout, including the nose up angle of disk-wing, the length of the blade, rotation speed, pitch angle and other parameters, are theoretically ana lyzed and experimentally tested. Calculation and experiments show that the shift process of the lift, the power and controls are smooth, and the designed scheme is feasible.展开更多
文摘Objective: To investigate the correlation of annexin A2 expression with epithelial-mesenchymal transition (EMT) and extracellular matrix (ECM) degradation in bile duct carcinoma. Methods: A total of 60 patients with primary bile duct carcinoma who accepted bile duct carcinoma laparotomy in our hospital between April 2009 and May 2017 were selected. The bile duct carcinoma tissue and adjacent normal tissue were kept during operation and enrolled in bile duct cancer group (n=60) and normal control group (n=60). The protein expression of annexin A2, EMT marker molecules, MMPs subtypes and TIMPs subtypes in two groups of specimens were detected, and Pearson test was used to evaluate the correlation of annexin A2 expression with EMT and ECM degradation in bile duct carcinoma tissue. Results: Annexin A2 expression in bile duct cancer group was higher than that in normal control group;E-cadherin, β-catenin and syndecan-1 protein expression in bile duct cancer group were lower than those in control group whereas P-cadherin and Vimentin protein expression were higher than those in normal control group;MMP-2, MMP-7 and MMP-9 protein expression in bile duct cancer group were higher than those in normal control group whereas TIMP-1 and TIMP-2 protein expression were lower than those in normal control group. Pearson test showed that the annexin A2 expression in bile duct carcinoma was directly correlated with the protein expression of EMT marker molecules, MMPs subtypes and TIMPs subtypes. Conclusion: annexin A2 increases in bile duct carcinoma, and it can regulate EMT and ECM degradation process to affect the disease outcome.
基金Supported by Ministry of Education,Culture,Sports Science,and Technology,and the Ministry of Health,Labour and Welfare of Japan
文摘Pancreatic cancer continues to be a leading cause of cancer-related death worldwide and there is an urgent need to develop novel diagnostic and therapeutic strategies to reduce the mortality of patients with this disease. In pancreatic cancer, some tight junction proteins, including claudins, are abnormally regulated and therefore are promising molecular targets for diagnosis, prognosis and therapy. Claudin-4 and-18 are overexpressed in human pancreatic cancer and its precursor lesions. Claudin-4 is a high affinity receptor of Clostridium perfringens enterotoxin(CPE). The cytotoxic effects of CPE and monoclonal antibodies against claudin-4 are useful as novel therapeutic tools for pancreatic cancer. Claudin-18 could be a putative marker and therapeutic target with prognostic implications for patients with pancreatic cancer. Claudin-1,-7, tricellulin and marvelD3 are involved in epithelial to mesenchymal transition(EMT) of pancreatic cancer cells and thus might be useful as biomarkers during disease. Protein kinase C is closely related to EMT of pancreatic cancer and regulates tight junctions of normal human pancreatic duct epithelial cells and the cancer cells. This review focuses on the regulation of tight junctions via protein kinase C during EMT in human pancreatic cancer for the purpose of developing new diagnostic and therapeutic modalities for pancreatic cancer.
文摘To analyze the existing schemes of high-speed rotorcrafts and some new technologies, a new conceptual sketch of the high-speed rotor/wing transition helicopter RD15 is proposed. The overall layout of the RD15 is given out and the transition process from the helicopter mode to the airplane mode is designed. The lift system consists of a circular disk-wing with four retractable blades. The technology of individual blade control is adopted for flight control in hover and low speed flight. The tail is a vectored thrust duct propeller. It can provide the anti-torque in hover, and offer the multi-directional controls and propulsion drive for the airplane mode flight. The aerodynamic characteristics and key technologies in the transition process for this layout, including the nose up angle of disk-wing, the length of the blade, rotation speed, pitch angle and other parameters, are theoretically ana lyzed and experimentally tested. Calculation and experiments show that the shift process of the lift, the power and controls are smooth, and the designed scheme is feasible.
