The number of the circulating angiogenic cells （CACs） and colony forming units （CFUs） derived from cultured circulating mononuclear cells （MNCs） represents a laboratory surrogate for endothelial cell repair abil...The number of the circulating angiogenic cells （CACs） and colony forming units （CFUs） derived from cultured circulating mononuclear cells （MNCs） represents a laboratory surrogate for endothelial cell repair ability. The serum of men with erectile dysfunction （ED） and vascular risk factors （VRFs） showed an increased level of endothelial cell damage/dysfunction markers and reduced the numbers of CACs and CFUs derived from the cells of healthy men. We analyzed whether treating men with ED and VRFs with the selective phosphodiesterase type 5 inhibitor tadalafil improved the endothelial cell repair ability and reduced the levels of the serum markers of endothelial cell damage/dysfunction. MNCs from healthy men were cultured with 20% serum from 36 ED patients to obtain CACs and CFUs. The ED patients were evaluated before and after 4weeks of treatment with tadalafil （20 mg every other day） or with a placebo. The tadalafil treatment improved erectile function （P = 0.0028）, but had no effect on the inhibitory effects of serum from ED patients on the CACs and CFUs derived from healthy men. The levels of endothelin-1 （P = 0.011） and tissue type plasminogen activator （P = 0.005） were reduced after treatment compared to baseline and those of the placebo group, whereas no changes were observed in the E-selectin levels. The tadalafil treatment in the ED patients with VRFs resulted in only a modest effect on the laboratory measures of the endothelial cell damage/dysfunction and repair ability. The proposed beneficial effect of phosphodiesterase type 5 inhibition on vascular homeostasis requires further analysis.展开更多
Burn wounds result in varying degrees of soft tissue damage that are typically graded clinically.Recently a key participant in neovascularization,the endothelial progenitor cel,has been the subject of intense cardiova...Burn wounds result in varying degrees of soft tissue damage that are typically graded clinically.Recently a key participant in neovascularization,the endothelial progenitor cel,has been the subject of intense cardiovascular research to explore whether it can serve as a biomarker for vascular injury.In this review,we examine the identity of the endothelial progenitor cell as well as the evidence that support its role as a key responder after burn insult.While there is conflicting evidence with regards to the delta of endothelial progenitor cell mobilization and burn severity,it is clear that they play an important role in wound healing.Systematic and controlled studies are needed to clarify this relationship,and whether this population can serve as a biomarker for burn severity.展开更多
文摘The number of the circulating angiogenic cells （CACs） and colony forming units （CFUs） derived from cultured circulating mononuclear cells （MNCs） represents a laboratory surrogate for endothelial cell repair ability. The serum of men with erectile dysfunction （ED） and vascular risk factors （VRFs） showed an increased level of endothelial cell damage/dysfunction markers and reduced the numbers of CACs and CFUs derived from the cells of healthy men. We analyzed whether treating men with ED and VRFs with the selective phosphodiesterase type 5 inhibitor tadalafil improved the endothelial cell repair ability and reduced the levels of the serum markers of endothelial cell damage/dysfunction. MNCs from healthy men were cultured with 20% serum from 36 ED patients to obtain CACs and CFUs. The ED patients were evaluated before and after 4weeks of treatment with tadalafil （20 mg every other day） or with a placebo. The tadalafil treatment improved erectile function （P = 0.0028）, but had no effect on the inhibitory effects of serum from ED patients on the CACs and CFUs derived from healthy men. The levels of endothelin-1 （P = 0.011） and tissue type plasminogen activator （P = 0.005） were reduced after treatment compared to baseline and those of the placebo group, whereas no changes were observed in the E-selectin levels. The tadalafil treatment in the ED patients with VRFs resulted in only a modest effect on the laboratory measures of the endothelial cell damage/dysfunction and repair ability. The proposed beneficial effect of phosphodiesterase type 5 inhibition on vascular homeostasis requires further analysis.
基金This work was supported in part by the California Institute for Regenerative Medicine training grant award TG2-012252 from the University of California,Irvine
文摘Burn wounds result in varying degrees of soft tissue damage that are typically graded clinically.Recently a key participant in neovascularization,the endothelial progenitor cel,has been the subject of intense cardiovascular research to explore whether it can serve as a biomarker for vascular injury.In this review,we examine the identity of the endothelial progenitor cell as well as the evidence that support its role as a key responder after burn insult.While there is conflicting evidence with regards to the delta of endothelial progenitor cell mobilization and burn severity,it is clear that they play an important role in wound healing.Systematic and controlled studies are needed to clarify this relationship,and whether this population can serve as a biomarker for burn severity.
