In vitro Screening of Fusarium Wilt-resistant Germplasm Resources of Red Edible Seed Watermelon( Citrullus lanatus ssp. vulgaris var. megalaspermus Lin et Chao)

[ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric acid, thus providing resistant materials for breeding Fusarium wilt-resistant red edible seed watermel- on. [ Method] Using Zhongxin No. 1 red edible seed watermelon advemitious buds as screening materials and fusaric acid （FA） as a stress agent, in vitro screen- ing of Fusarium wilt-resistant red edible seed watermelon clonal variants and identification of Fusarium wilt-resistance of the germplasm resources of red edible seed watermelon were performed. [ Result] The results showed that the appropriate FA for in vitro screening of Fusarium wilt-resistant red edible seed watermelon vari- ants was 15 mg/L. In vitro screening system for Fusarium wilt-resistant red edible seed watermelon variants was established preliminarily and FA-resistant regenera- ted plants were obtained. Among the 36 germplasm resources of red edible seed watermelon, there were 2 highly resistant materials, 6 moderately resistant materi- als, 11 slightly resistant materials and 17 highly susceptible materials. [ Conclusion] This study confirmed preliminarily that in vitro screening method is effective for obtaining resistant materials of red edible seed watermelon.

籽用西瓜种质资源抗枯萎病离体筛选技术的研究

以籽用西瓜中信1号红籽瓜不定芽为筛选材料,利用镰刀菌酸(Fusariumacid,FA)为胁迫剂,进行红籽瓜抗枯萎病无性系变异体离体筛选技术和红籽瓜种质资源抗病性鉴定的研究。结果表明,红籽瓜体细胞无性系抗性变异体离体筛选的FA适宜浓度为15mg.L-1。初步建立了红籽瓜抗性变异体离体筛选体系,获得了抗FA变异体的再生植株。筛选出红籽瓜种质资源高抗材料2份,中抗材料6份,轻抗材料11份,高感材料17份。