[ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric ...[ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric acid, thus providing resistant materials for breeding Fusarium wilt-resistant red edible seed watermel- on. [ Method] Using Zhongxin No. 1 red edible seed watermelon advemitious buds as screening materials and fusaric acid （FA） as a stress agent, in vitro screen- ing of Fusarium wilt-resistant red edible seed watermelon clonal variants and identification of Fusarium wilt-resistance of the germplasm resources of red edible seed watermelon were performed. [ Result] The results showed that the appropriate FA for in vitro screening of Fusarium wilt-resistant red edible seed watermelon vari- ants was 15 mg/L. In vitro screening system for Fusarium wilt-resistant red edible seed watermelon variants was established preliminarily and FA-resistant regenera- ted plants were obtained. Among the 36 germplasm resources of red edible seed watermelon, there were 2 highly resistant materials, 6 moderately resistant materi- als, 11 slightly resistant materials and 17 highly susceptible materials. [ Conclusion] This study confirmed preliminarily that in vitro screening method is effective for obtaining resistant materials of red edible seed watermelon.展开更多
Silicon deposition in leaf trichome of six horticultural Cucurbitaceae species, cucumber (Cucumis sativus), pumpkin (Cucurbita maxima), melon (Cucumis melo), watermelon (Citrullus lanatus), sponge gourd (Luffa cylindr...Silicon deposition in leaf trichome of six horticultural Cucurbitaceae species, cucumber (Cucumis sativus), pumpkin (Cucurbita maxima), melon (Cucumis melo), watermelon (Citrullus lanatus), sponge gourd (Luffa cylindrica) and bottle gourd (Lagenaria siceraria var. hispida) was observed by an X-ray microanalyzer coupled with an environmental scanning electron microscope. The elements that presented in the surface of three or four leaves of the individual species were detected and mapped by the X-ray microanalyzer. In leaves of cucumber, pumpkin, and melon, high accumulation of silicon was detected in cells surrounding the bases of the trichome hair and the hair itself deposited calcium. On the other hand, in sponge gourd and bottle gourd, high accumulation of silicon was detected only in the hair. In watermelon leaves, silicon deposited both in the hair and in cells surrounding the bases of the hair. Thus, horticultural Cucurbitaceae plants have interspecific variation in the pattern of silicon deposition in leaf trichomes.展开更多
基金Supported by National Space Breeding Project of China(2006HT100113)China Agriculture Research System(CARS-26)Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(GNK2013YZ26)
文摘[ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric acid, thus providing resistant materials for breeding Fusarium wilt-resistant red edible seed watermel- on. [ Method] Using Zhongxin No. 1 red edible seed watermelon advemitious buds as screening materials and fusaric acid （FA） as a stress agent, in vitro screen- ing of Fusarium wilt-resistant red edible seed watermelon clonal variants and identification of Fusarium wilt-resistance of the germplasm resources of red edible seed watermelon were performed. [ Result] The results showed that the appropriate FA for in vitro screening of Fusarium wilt-resistant red edible seed watermelon vari- ants was 15 mg/L. In vitro screening system for Fusarium wilt-resistant red edible seed watermelon variants was established preliminarily and FA-resistant regenera- ted plants were obtained. Among the 36 germplasm resources of red edible seed watermelon, there were 2 highly resistant materials, 6 moderately resistant materi- als, 11 slightly resistant materials and 17 highly susceptible materials. [ Conclusion] This study confirmed preliminarily that in vitro screening method is effective for obtaining resistant materials of red edible seed watermelon.
文摘Silicon deposition in leaf trichome of six horticultural Cucurbitaceae species, cucumber (Cucumis sativus), pumpkin (Cucurbita maxima), melon (Cucumis melo), watermelon (Citrullus lanatus), sponge gourd (Luffa cylindrica) and bottle gourd (Lagenaria siceraria var. hispida) was observed by an X-ray microanalyzer coupled with an environmental scanning electron microscope. The elements that presented in the surface of three or four leaves of the individual species were detected and mapped by the X-ray microanalyzer. In leaves of cucumber, pumpkin, and melon, high accumulation of silicon was detected in cells surrounding the bases of the trichome hair and the hair itself deposited calcium. On the other hand, in sponge gourd and bottle gourd, high accumulation of silicon was detected only in the hair. In watermelon leaves, silicon deposited both in the hair and in cells surrounding the bases of the hair. Thus, horticultural Cucurbitaceae plants have interspecific variation in the pattern of silicon deposition in leaf trichomes.
