[ Objective ] This study aimed to establish the infrared （IR） spectra fingerprints for peels of Citrus grandis from Guangxi Zhuang Autonomous Region. [ Method ] The fingerprint was established by fourier transform i...[ Objective ] This study aimed to establish the infrared （IR） spectra fingerprints for peels of Citrus grandis from Guangxi Zhuang Autonomous Region. [ Method ] The fingerprint was established by fourier transform infrared spectroscopy （ FT-IR）, and then were analyzed by Omic software. [ Result ] IR spectra fin- gerprint of C. grand/s were successfully established. There were six common peaks in the characteristic spectra of 12 samples. [ Conclusion] The specific, rapid and stable method can be applied to quality control of C. grandis.展开更多
[Objective] The aim was to identify genetic variation in Citrus sinensis （sweet orange） germplasm from Hunan Province according to the Start Codon Targeted （SCoT） Polymorphism. [Method] The reaction system for SCo...[Objective] The aim was to identify genetic variation in Citrus sinensis （sweet orange） germplasm from Hunan Province according to the Start Codon Targeted （SCoT） Polymorphism. [Method] The reaction system for SCoT amplification from sweet orange was first optimized, and then the SCoT fragments were amplified from 24 sweet orange cultivars collected in Hunan Province and sequenced for genetic variation analysis. [Result] The optimum reaction system for SCoT markers amplification was 2.0 μl containing 80 ng of template DNA, 0.3 mmol/L dNTPs, 0.2 μmol/L primer, 1.6 mmol/L Mg2＋, 1.6 U of Taq DNA polymerase and 10×PCR buffer. By using this reaction system, the PCR products from the sweet orange cultivars produced clear and reproducible bands at 100-2 000 bp through electrophoresis. The SCoT fragments of the 24 sweet orange cultivars were 1 090-1 091 bp, with the homology of 99.84% and nucleotide deletion and substitution. After being sequenced, the SCoT polymorphisms could distinguish 12 sweet orange cultivars. In addition, the BLAST result showed that part of the SCoT fragments coding region shared high homology with ribosomal protein S3 N superfamily. [Conclusion] This study will provide a theoretical basis for breeding sweet orange cultivars.展开更多
In-vitro callus induction and regeneration method was developed using different plant growth regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and Gamborg (B5) media) of Citrus sinensis (L.) Osbec...In-vitro callus induction and regeneration method was developed using different plant growth regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and Gamborg (B5) media) of Citrus sinensis (L.) Osbeck. Observations of the effect of PGRs were carried out using different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D),1-naphthalene acetic acid (NAA) and combinations of 2,4-D and NAA using different basal media. This study found Citrus sinensis (L.) Osbeck exhibited a high frequency of callus induction on MS medium supplemented with 3 mg/L 2,4-D and callus induction frequency was 86.7% ± 3.4% whereas N6 and B5 showed lower callus induction frequency of 83.3% ± 8.8% and 82.2% ± 1.9% respectively compared to that of MS media with supplementation of the same hormone. Among the induced calli, the morphological analysis showed only 40% - 50% was embryogenic calli. Regeneration of plantlets from calli was done using different concentrations and combinations of auxin and cytokinin. The study showed that 3 mg/L 6-benzylaminopurine (BAP) supplemented medium has the maximum potential to promote regeneration of Citrus sinensis (L.) Osbeck from embryogenic calli with the frequency of 89.3% ± 8.8% but no regeneration occurred from the non-embryogenic calli. The regenerated plantlets were rooted on MS medium with supplementation of 5 mg/l NAA. These observations in Citrus sinensis (L.) Osbeck regeneration will be helpful for genetic improvement with desired traits.展开更多
To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis(L.)Osbeck and citrus grandis(L.)Osbeck by the way of HPLC.With shimadzu vp-ODS(4.6 mm×150 mm,5 μm) colum ...To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis(L.)Osbeck and citrus grandis(L.)Osbeck by the way of HPLC.With shimadzu vp-ODS(4.6 mm×150 mm,5 μm) colum and the mobile phase is CH3CN-(H3PO4.pH=3.5)(22︰78),the velocity of flow is 1.20mL.min-1and the detective wavelength is favourable 283 nm.The separate degree between the determination component and other components is favourable(R>1.5),the linearity rang of hesperidin and naringin are 0.022μg^1.80 μg and 0.023μg^1.90 μg respectively,The average recoveries of hesperidin and naringin are 99.3%,97.6% respectively and their RSD are 1.4%,2.3% respectively.Conclusion:This method is convenient and easy,its result can be used to afford the feasibility to confirm material of the two active components.展开更多
以易发生汁胞粒化的老龄树和不易发生汁胞粒化的适龄树的琯溪蜜柚(Citrus grand is(L.) Osbeck‘Guanxi-miyou’)果实为材料,研究了果实成熟过程中汁胞粒化发生与细胞壁代谢的关系。结果表明:老龄树果实的汁胞粒化指数随着果实成熟而上...以易发生汁胞粒化的老龄树和不易发生汁胞粒化的适龄树的琯溪蜜柚(Citrus grand is(L.) Osbeck‘Guanxi-miyou’)果实为材料,研究了果实成熟过程中汁胞粒化发生与细胞壁代谢的关系。结果表明:老龄树果实的汁胞粒化指数随着果实成熟而上升。在汁胞粒化发生过程中,汁胞维持较低的细胞壁降解酶[果胶甲酯酶(PE)、多聚半乳糖醛酸酶(PG)、纤维素酶(Cx)]活性,保持较高的细胞壁物质(原果胶、纤维素、半纤维素)含量;尤其在汁胞粒化的起动阶段和加快阶段,纤维素、半纤维素含量极显著增加。相反,适龄树果实的汁胞粒化指数在果实成熟过程中变化不大,汁胞中细胞壁降解酶活性较高,促进原果胶、纤维素、半纤维素等细胞壁物质的降解,保持较低的细胞壁物质含量,使汁胞发育正常、柔软多汁。这说明PE、PG、Cx活性和原果胶、纤维素、半纤维素含量与琯溪蜜柚汁胞粒化密切相关。展开更多
Pomelo,a species of Citrus,has been developed tremendously as a kind of fruit in China in recent decades.There is disagreement with the origin and evolution of pomelo .Here the author gave some comments on the natural...Pomelo,a species of Citrus,has been developed tremendously as a kind of fruit in China in recent decades.There is disagreement with the origin and evolution of pomelo .Here the author gave some comments on the natural differentiation of pomelo and the flux of the centre of genetic diversity, by summing up the studies on the genetic diversity of pomelo and the distribution of almost 70 cultivars in China.The author also pointed out some problems about the research into the biology of pomelo germplasm.展开更多
基金Supported by the Natural Science Foundation of Guangxi Zhuang Autonomous Region (2011GXNSFF018006)
文摘[ Objective ] This study aimed to establish the infrared （IR） spectra fingerprints for peels of Citrus grandis from Guangxi Zhuang Autonomous Region. [ Method ] The fingerprint was established by fourier transform infrared spectroscopy （ FT-IR）, and then were analyzed by Omic software. [ Result ] IR spectra fin- gerprint of C. grand/s were successfully established. There were six common peaks in the characteristic spectra of 12 samples. [ Conclusion] The specific, rapid and stable method can be applied to quality control of C. grandis.
基金Supported by National Key Technology Research and Development Program(2006BAD01A1702)~~
文摘[Objective] The aim was to identify genetic variation in Citrus sinensis （sweet orange） germplasm from Hunan Province according to the Start Codon Targeted （SCoT） Polymorphism. [Method] The reaction system for SCoT amplification from sweet orange was first optimized, and then the SCoT fragments were amplified from 24 sweet orange cultivars collected in Hunan Province and sequenced for genetic variation analysis. [Result] The optimum reaction system for SCoT markers amplification was 2.0 μl containing 80 ng of template DNA, 0.3 mmol/L dNTPs, 0.2 μmol/L primer, 1.6 mmol/L Mg2＋, 1.6 U of Taq DNA polymerase and 10×PCR buffer. By using this reaction system, the PCR products from the sweet orange cultivars produced clear and reproducible bands at 100-2 000 bp through electrophoresis. The SCoT fragments of the 24 sweet orange cultivars were 1 090-1 091 bp, with the homology of 99.84% and nucleotide deletion and substitution. After being sequenced, the SCoT polymorphisms could distinguish 12 sweet orange cultivars. In addition, the BLAST result showed that part of the SCoT fragments coding region shared high homology with ribosomal protein S3 N superfamily. [Conclusion] This study will provide a theoretical basis for breeding sweet orange cultivars.
文摘In-vitro callus induction and regeneration method was developed using different plant growth regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and Gamborg (B5) media) of Citrus sinensis (L.) Osbeck. Observations of the effect of PGRs were carried out using different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D),1-naphthalene acetic acid (NAA) and combinations of 2,4-D and NAA using different basal media. This study found Citrus sinensis (L.) Osbeck exhibited a high frequency of callus induction on MS medium supplemented with 3 mg/L 2,4-D and callus induction frequency was 86.7% ± 3.4% whereas N6 and B5 showed lower callus induction frequency of 83.3% ± 8.8% and 82.2% ± 1.9% respectively compared to that of MS media with supplementation of the same hormone. Among the induced calli, the morphological analysis showed only 40% - 50% was embryogenic calli. Regeneration of plantlets from calli was done using different concentrations and combinations of auxin and cytokinin. The study showed that 3 mg/L 6-benzylaminopurine (BAP) supplemented medium has the maximum potential to promote regeneration of Citrus sinensis (L.) Osbeck from embryogenic calli with the frequency of 89.3% ± 8.8% but no regeneration occurred from the non-embryogenic calli. The regenerated plantlets were rooted on MS medium with supplementation of 5 mg/l NAA. These observations in Citrus sinensis (L.) Osbeck regeneration will be helpful for genetic improvement with desired traits.
文摘To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis(L.)Osbeck and citrus grandis(L.)Osbeck by the way of HPLC.With shimadzu vp-ODS(4.6 mm×150 mm,5 μm) colum and the mobile phase is CH3CN-(H3PO4.pH=3.5)(22︰78),the velocity of flow is 1.20mL.min-1and the detective wavelength is favourable 283 nm.The separate degree between the determination component and other components is favourable(R>1.5),the linearity rang of hesperidin and naringin are 0.022μg^1.80 μg and 0.023μg^1.90 μg respectively,The average recoveries of hesperidin and naringin are 99.3%,97.6% respectively and their RSD are 1.4%,2.3% respectively.Conclusion:This method is convenient and easy,its result can be used to afford the feasibility to confirm material of the two active components.
文摘以易发生汁胞粒化的老龄树和不易发生汁胞粒化的适龄树的琯溪蜜柚(Citrus grand is(L.) Osbeck‘Guanxi-miyou’)果实为材料,研究了果实成熟过程中汁胞粒化发生与细胞壁代谢的关系。结果表明:老龄树果实的汁胞粒化指数随着果实成熟而上升。在汁胞粒化发生过程中,汁胞维持较低的细胞壁降解酶[果胶甲酯酶(PE)、多聚半乳糖醛酸酶(PG)、纤维素酶(Cx)]活性,保持较高的细胞壁物质(原果胶、纤维素、半纤维素)含量;尤其在汁胞粒化的起动阶段和加快阶段,纤维素、半纤维素含量极显著增加。相反,适龄树果实的汁胞粒化指数在果实成熟过程中变化不大,汁胞中细胞壁降解酶活性较高,促进原果胶、纤维素、半纤维素等细胞壁物质的降解,保持较低的细胞壁物质含量,使汁胞发育正常、柔软多汁。这说明PE、PG、Cx活性和原果胶、纤维素、半纤维素含量与琯溪蜜柚汁胞粒化密切相关。
文摘Pomelo,a species of Citrus,has been developed tremendously as a kind of fruit in China in recent decades.There is disagreement with the origin and evolution of pomelo .Here the author gave some comments on the natural differentiation of pomelo and the flux of the centre of genetic diversity, by summing up the studies on the genetic diversity of pomelo and the distribution of almost 70 cultivars in China.The author also pointed out some problems about the research into the biology of pomelo germplasm.
