Objective:In this study,black tea and Citrus maxima(BT-CM),yellow tea and C.maxima(YT-CM),green tea and C.maxima(GT-CM)as subjects,the active ingredient content and antioxidant activity of three tea and C.maxima(T-CM)...Objective:In this study,black tea and Citrus maxima(BT-CM),yellow tea and C.maxima(YT-CM),green tea and C.maxima(GT-CM)as subjects,the active ingredient content and antioxidant activity of three tea and C.maxima(T-CM)were analyzed.The effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further explored.Methods:National standard method and HPLC were used for active ingredient analysis.MTT,cell fow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation,apoptosis,and its underlying molecular mechanism.Results:The content of tea polyphenols,free amino acids,ratio of polyphenols and amino acids,ester catechins,non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of BT-CM.The in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of BT-CM.Three T-CMs had the effects of inhibiting proliferation,arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells,especially YT-CM and GT-CM.Western blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax,Bcl-2 and Caspase-3/9.YT-CM and GT-CM had better ability to change the signal pathway than BT-CM.Conclusion:In short,T-CMs,which combined different degrees of fermentation tea with C.maxima,were rich in nutrients and biologically active substances.T-CMs,especially YT-CM and GT-CM,are healthy drinks that help to prevent and treat liver cancer.展开更多
【目的】评价意大利蜜蜂 Apis mellifera ligustica (简称“意蜂”)及中华蜜蜂 Apis cerana cerana (简称“中蜂”)对黄沙白柚 Citrus maxima (Burm) Merr. cv. huangsha Yu的访花行为及授粉效果。【方法】于重庆市垫江县开展2种蜂对黄...【目的】评价意大利蜜蜂 Apis mellifera ligustica (简称“意蜂”)及中华蜜蜂 Apis cerana cerana (简称“中蜂”)对黄沙白柚 Citrus maxima (Burm) Merr. cv. huangsha Yu的访花行为及授粉效果。【方法】于重庆市垫江县开展2种蜂对黄沙白柚的访花行为及授粉效果试验,比较2种蜂访问黄沙白柚花朵的行为及其与环境因素和花朵泌蜜规律的相关性,并评价意蜂与中蜂授粉对黄沙白柚座果率、产量及果实品质的影响。【结果】意蜂与中蜂访问黄沙白柚花朵的数量呈先上升后下降的趋势,在13:00-17:00时间段中蜂访问黄沙白柚花朵的数量显著高于意蜂( P <0.05),但意蜂的单花访问时间显著高于中蜂( P <0.05);意蜂访问黄沙白柚花朵的数量受花朵泌蜜量和光照显著影响( P <0.05),中蜂的访花数量受花蜜糖浓度和光照显著影响( P <0.05)。经意蜂与中蜂授粉的黄沙白柚果实座果率、单果重、果型及果实品质显著高于自花授粉组( P <0.05),且意蜂授粉组的座果率及2种蜂授粉组的总糖含量和糖酸比均显著高于人工授粉组( P <0.05);意蜂授粉组和中蜂授粉组总果重无显著差异( P >0.05),但意蜂授粉组显著高于人工授粉组与自花授粉组( P <0.05);与中蜂授粉相比,意蜂授粉能够显著提高黄沙白柚的果实座果率。【结论】综合比较2种蜂对黄沙白柚的访花行为及授粉效果,黄沙白柚可采用蜜蜂授粉代替人工授粉,且意蜂授粉效果更好。展开更多
基金supported by the"14th Five-Year Plan"teambuilding projects of Guangdong Academy of Agricultural Sciences[202126TD]Guangdong Basic and Applied Basic Research Foundation[2020A1515011266,2021A1515010958]+3 种基金Guangzhou Science and Technology Plan Projects[202002030202,202102020047]Key-Area Research and Development Program of Guangdong Province[2020B0202080003]Innovation Fund projects of Guangdong Academy of Agricultural Sciences[202115,202035]Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science[R2019PY-JX004]
文摘Objective:In this study,black tea and Citrus maxima(BT-CM),yellow tea and C.maxima(YT-CM),green tea and C.maxima(GT-CM)as subjects,the active ingredient content and antioxidant activity of three tea and C.maxima(T-CM)were analyzed.The effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further explored.Methods:National standard method and HPLC were used for active ingredient analysis.MTT,cell fow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation,apoptosis,and its underlying molecular mechanism.Results:The content of tea polyphenols,free amino acids,ratio of polyphenols and amino acids,ester catechins,non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of BT-CM.The in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of BT-CM.Three T-CMs had the effects of inhibiting proliferation,arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells,especially YT-CM and GT-CM.Western blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax,Bcl-2 and Caspase-3/9.YT-CM and GT-CM had better ability to change the signal pathway than BT-CM.Conclusion:In short,T-CMs,which combined different degrees of fermentation tea with C.maxima,were rich in nutrients and biologically active substances.T-CMs,especially YT-CM and GT-CM,are healthy drinks that help to prevent and treat liver cancer.
文摘【目的】评价意大利蜜蜂 Apis mellifera ligustica (简称“意蜂”)及中华蜜蜂 Apis cerana cerana (简称“中蜂”)对黄沙白柚 Citrus maxima (Burm) Merr. cv. huangsha Yu的访花行为及授粉效果。【方法】于重庆市垫江县开展2种蜂对黄沙白柚的访花行为及授粉效果试验,比较2种蜂访问黄沙白柚花朵的行为及其与环境因素和花朵泌蜜规律的相关性,并评价意蜂与中蜂授粉对黄沙白柚座果率、产量及果实品质的影响。【结果】意蜂与中蜂访问黄沙白柚花朵的数量呈先上升后下降的趋势,在13:00-17:00时间段中蜂访问黄沙白柚花朵的数量显著高于意蜂( P <0.05),但意蜂的单花访问时间显著高于中蜂( P <0.05);意蜂访问黄沙白柚花朵的数量受花朵泌蜜量和光照显著影响( P <0.05),中蜂的访花数量受花蜜糖浓度和光照显著影响( P <0.05)。经意蜂与中蜂授粉的黄沙白柚果实座果率、单果重、果型及果实品质显著高于自花授粉组( P <0.05),且意蜂授粉组的座果率及2种蜂授粉组的总糖含量和糖酸比均显著高于人工授粉组( P <0.05);意蜂授粉组和中蜂授粉组总果重无显著差异( P >0.05),但意蜂授粉组显著高于人工授粉组与自花授粉组( P <0.05);与中蜂授粉相比,意蜂授粉能够显著提高黄沙白柚的果实座果率。【结论】综合比较2种蜂对黄沙白柚的访花行为及授粉效果,黄沙白柚可采用蜜蜂授粉代替人工授粉,且意蜂授粉效果更好。
