This research aimed to get the best composition ofcytokinin （BAP） and auxin （NAA） concentration on in vitro axillary bud multiplication of Tawangmangu orange （Citrus nobilis Lour.）. The research was conducted in...This research aimed to get the best composition ofcytokinin （BAP） and auxin （NAA） concentration on in vitro axillary bud multiplication of Tawangmangu orange （Citrus nobilis Lour.）. The research was conducted in Plant Physiology and Biotechnology Laboratory of Agriculture Faculty, Sebelas Maret University Surakarta from February 2008 to September 2008, used factorial design with two factors based on Completely Random Design. The first factor was BAP concentration consisting of five levels： 0, 0.5, 1, 1.5, 2 ppm and the second factor was NAA concentration consisting of five levels： 0, 0.5, 1, 1.5, 2 ppm. So there were 25 combination treatments, each of them was repeated for three times. The result observation data of shoot initiation time and number of shoot were analyzed with analysis of variance and if significant continued with Duncan＇s Multiple Range Test at 5% level. Data of shoot color and amount of leaves were analyzed descriptively. The result showed that there was interaction between BAP and NAA on shoot initiation time. Combination of 0.5 ppm BAP and 1.5 ppm NAA was able to accelerate the shoot initiation time and produced the largest number of leaves that was eight leaves. BAP was able to increase the number of shoots. Average largest amount of shoot on 0.5 ppm BAP treatment was 3.93 shoots/explant. The dominant color of leaves was light green.展开更多
文摘This research aimed to get the best composition ofcytokinin （BAP） and auxin （NAA） concentration on in vitro axillary bud multiplication of Tawangmangu orange （Citrus nobilis Lour.）. The research was conducted in Plant Physiology and Biotechnology Laboratory of Agriculture Faculty, Sebelas Maret University Surakarta from February 2008 to September 2008, used factorial design with two factors based on Completely Random Design. The first factor was BAP concentration consisting of five levels： 0, 0.5, 1, 1.5, 2 ppm and the second factor was NAA concentration consisting of five levels： 0, 0.5, 1, 1.5, 2 ppm. So there were 25 combination treatments, each of them was repeated for three times. The result observation data of shoot initiation time and number of shoot were analyzed with analysis of variance and if significant continued with Duncan＇s Multiple Range Test at 5% level. Data of shoot color and amount of leaves were analyzed descriptively. The result showed that there was interaction between BAP and NAA on shoot initiation time. Combination of 0.5 ppm BAP and 1.5 ppm NAA was able to accelerate the shoot initiation time and produced the largest number of leaves that was eight leaves. BAP was able to increase the number of shoots. Average largest amount of shoot on 0.5 ppm BAP treatment was 3.93 shoots/explant. The dominant color of leaves was light green.
