Effects of Climatic Suitability on Clamworm Breeding along Jiangsu Coast of China

Data were collected by clamworm breeding and investigation and then analyzed to understand the changes in salinity and pH value caused by rainfall as well as climatic suitability along Jiangsu coast of China. The main climatic factors affecting clamworm breeding included water temperature, strong wind and heavy rainfall. The period with air temperature stable above 14 ℃ was suitable for clamworm breeding, and it was from eady Apr. to mid-Nov, along Jiangsu coast. The period with air temperature stable above 30 ℃ in summer was unsuitable for clamworm breeding, and it was from mid-Jul, to late Jul. along Jiangsu coast. During the suitable period for clamworm breeding, the annual days of strong wind and those of heavy rainfall were 4.3 - 39.2 and 2.9 - 4.0 d, respectively. The effects of rainfall （or water exchange） on pH values were determined by the proportion of rainfall （or water exchange） in total pond water and the difference between their pH values. The climate along Jiangsu coast was suitable for clamworm breeding, and the climatic suitability decreased successively in Qidong, Lusi, Ganyu, Rudong, Dafeng, Sheyang, Yanweigang, and Western Liandao. Therefore, it is important to evaluate the effects of severe weather, forecast disastrous weather and formulate rational measurements for flood control and disaster relief.

沙蚕丝氨酸蛋白酶家族相关基因的克隆和测序

①目的 从海洋生物双齿围沙蚕消化道上皮细胞中获得编码丝氨酸内肽酶某一功能基因的克隆。②方法 以丝氨酸内肽酶高度保守位点设计引物 ,利用 5′cDNA末端快速扩增 (5′RACE)和 3′RACE的方法 ,分别获得该保守位点前后的片段 ,克隆、测序。③结果 获得一条长度为 5 12bp编码丝氨酸内肽酶相关基因的序列。

对出口鲜活沙蚕重量检验工作的几点思考

鲜活沙蚕含水量高,其重量极易受盐度、温度等环境因素的影响,同时对包装状态,储运时间也十分敏感,做好鲜活沙蚕的重量检验工作,有效维护贸易双方的合法权益具有重要的现实意义。本文分析了影响出口鲜活沙蚕重量检验准确性的关键因素,并针对性的提出了注意事项及改进措施。

一种新的沙蚕纤溶酶的纯化、活性鉴定及部分分子特性

目的:从沙蚕Nereis virens的体腔液中纯化、鉴定出一种新的纤溶酶,并对其进行分子表征。方法:运用离子交换层析、凝胶过滤层析等常规色谱方法纯化蛋白酶,采用纤维蛋白板检测法鉴定纤溶活性及活性分布曲线,利用2D-电泳方法检测此纤溶酶的表观分子量和等电点,并检测多种蛋白酶抑制剂对其酶活性的影响,以确定此蛋白酶的类型。结果:获取一种新的具有极强纤维蛋白水解活性的单链蛋白酶,其相对分子质量为29000,等电点为4.5,其蛋白水解活性在pH7.8和45℃时表现为最高,并能被二异丙基氟磷酸(DFP)和甲苯磺酚氟(PMSF)完全性抑制,确证其为一种典型的丝氨酸内肽酶。结论:Nereis virens的体腔液中存在一种纤溶活性极强的单链丝氨酸内肽酶,该纤溶酶对预防和治疗血栓性疾病具有一定的药用价值。